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Q8SR37

- RIR1_ENCCU

UniProt

Q8SR37 - RIR1_ENCCU

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Protein
Ribonucleoside-diphosphate reductase large chain
Gene
ECU10_0920
Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding to separate specificity and activation sites on the large subunit. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction. Stimulated by ATP and inhibited by dATP binding to the activity site By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei196 – 1961Substrate By similarity
Sitei212 – 2121Important for hydrogen atom transfer By similarity
Sitei220 – 2201Allosteric effector binding, determines substrate specificity By similarity
Binding sitei241 – 2411Substrate; via amide nitrogen By similarity
Sitei250 – 2501Allosteric effector binding, determines substrate specificity By similarity
Active sitei420 – 4201Proton acceptor By similarity
Active sitei422 – 4221Cysteine radical intermediate By similarity
Active sitei424 – 4241Proton acceptor By similarity
Sitei437 – 4371Important for hydrogen atom transfer By similarity
Sitei729 – 7291Important for electron transfer By similarity
Sitei730 – 7301Important for electron transfer By similarity
Sitei763 – 7631Interacts with thioredoxin/glutaredoxin By similarity
Sitei766 – 7661Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
  2. deoxyribonucleotide biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large chain (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Ordered Locus Names:ECU10_0920
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
ProteomesiUP000000819: Chromosome X

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU10_0920.

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 768768Ribonucleoside-diphosphate reductase large chain
PRO_0000187200Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi212 ↔ 437Redox-active By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ8SR37.

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit By similarity.

Protein-protein interaction databases

STRINGi6035.ECU10_0920.

Structurei

3D structure databases

ProteinModelPortaliQ8SR37.
SMRiQ8SR37. Positions 79-734.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni211 – 2122Substrate binding By similarity
Regioni279 – 2824Allosteric effector binding, determines substrate specificity By similarity
Regioni420 – 4245Substrate binding By similarity
Regioni595 – 5995Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0209.
HOGENOMiHOG000057035.
KOiK10807.
OMAiDELIHQR.
OrthoDBiEOG7C5MHR.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8SR37-1 [UniParc]FASTAAdd to Basket

« Hide

MENFSNSKRI KSEKLGIDLI RSRIEGYVKD LKLRHVDVDE LVERIVSGWC    50
EDMTRKETID YCSETAASMI TKHPEYGQIS SRIIVSYIHE ITMDSFVEKI 100
KYIQKHRGMV SEEMYGIILE HGETIEGMIN YENDFLFSYF GILTLMKSYL 150
IKVGEEIIER PQDMFMRVAL QIHKTDFEKV REVYNLLSGH YFTHATPTLY 200
NSCLKNPQLA SCFLITPRED SIEGVYHMIN QAAIITKYSG GIGLNLHGIR 250
SKGSSLRSTG GRSNGIIPLI QVLNATKRYI NQGAERRPGS IAIFLEPWHM 300
EIFDFLELRK NTGPEEFRAR DIFTALWIND LFMERVKNNE EWSLFCPSQA 350
VGLSDVWGEE FNALYCKYEK TISRTVVPAQ KLWKAIIEAQ IETGTPYMCY 400
KDACNRLSNQ QHLGTIKSSN LCAEIVEYSS GEETSVCNLA SICLPMFVKD 450
GWFDFEAFRR VVKILTVNLN RVIDFNYYPV EEARRSNMRN RPIGIGVQGL 500
ADLFAILRLA FESDGARSLN QDIFEAMYYS AMEASCELAE KEGPFPSYEG 550
SPISKGIFHF ELAGRKASGN WDWEGLRERI RRHGVRNSLL IALMPTAGTS 600
QIFGNNEAFE PHASNIYTRR THAGEFQIVN QHLVNDLVRL GLWSYEMKNL 650
VIENEGSIQN ITSIPHEIRE IYKTAWEIKM KSVIDLAADR QVFVDQSQSL 700
NIFIAKPTYS QLTSMHFYGY HCGLKTGMYY LRTRPITSAI KFTVDKKLAE 750
KTLSSMNDTD DPCSMCSS 768
Length:768
Mass (Da):87,941
Last modified:June 1, 2002 - v1
Checksum:i3E6BF6826805E20D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590449 Genomic DNA. Translation: CAD25811.1.
RefSeqiNP_586207.1. NM_001042040.1.

Genome annotation databases

GeneIDi859856.
KEGGiecu:ECU10_0920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590449 Genomic DNA. Translation: CAD25811.1 .
RefSeqi NP_586207.1. NM_001042040.1.

3D structure databases

ProteinModelPortali Q8SR37.
SMRi Q8SR37. Positions 79-734.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 6035.ECU10_0920.

Proteomic databases

PRIDEi Q8SR37.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 859856.
KEGGi ecu:ECU10_0920.

Organism-specific databases

EuPathDBi MicrosporidiaDB:ECU10_0920.

Phylogenomic databases

eggNOGi COG0209.
HOGENOMi HOG000057035.
KOi K10807.
OMAi DELIHQR.
OrthoDBi EOG7C5MHR.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-M1.

Entry informationi

Entry nameiRIR1_ENCCU
AccessioniPrimary (citable) accession number: Q8SR37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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