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Protein

Ribonucleoside-diphosphate reductase large chain

Gene

ECU10_0920

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding to separate specificity and activation sites on the large subunit. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction. Stimulated by ATP and inhibited by dATP binding to the activity site (By similarity).By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei196SubstrateBy similarity1
Sitei212Important for hydrogen atom transferBy similarity1
Sitei220Allosteric effector binding, determines substrate specificityBy similarity1
Binding sitei241Substrate; via amide nitrogenBy similarity1
Sitei250Allosteric effector binding, determines substrate specificityBy similarity1
Active sitei420Proton acceptorBy similarity1
Active sitei422Cysteine radical intermediateBy similarity1
Active sitei424Proton acceptorBy similarity1
Sitei437Important for hydrogen atom transferBy similarity1
Sitei729Important for electron transferBy similarity1
Sitei730Important for electron transferBy similarity1
Sitei763Interacts with thioredoxin/glutaredoxinBy similarity1
Sitei766Interacts with thioredoxin/glutaredoxinBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large chain (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Ordered Locus Names:ECU10_0920
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
Proteomesi
  • UP000000819 Componenti: Chromosome X

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU10_0920.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872001 – 768Ribonucleoside-diphosphate reductase large chainAdd BLAST768

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi212 ↔ 437Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ8SR37.

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.By similarity

Protein-protein interaction databases

STRINGi284813.NP_586207.1.

Structurei

3D structure databases

ProteinModelPortaliQ8SR37.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni211 – 212Substrate bindingBy similarity2
Regioni279 – 282Allosteric effector binding, determines substrate specificityBy similarity4
Regioni420 – 424Substrate bindingBy similarity5
Regioni595 – 599Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1112. Eukaryota.
COG0209. LUCA.
HOGENOMiHOG000057035.
InParanoidiQ8SR37.
KOiK10807.
OMAiYELLWQM.
OrthoDBiEOG092C0QYB.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8SR37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENFSNSKRI KSEKLGIDLI RSRIEGYVKD LKLRHVDVDE LVERIVSGWC
60 70 80 90 100
EDMTRKETID YCSETAASMI TKHPEYGQIS SRIIVSYIHE ITMDSFVEKI
110 120 130 140 150
KYIQKHRGMV SEEMYGIILE HGETIEGMIN YENDFLFSYF GILTLMKSYL
160 170 180 190 200
IKVGEEIIER PQDMFMRVAL QIHKTDFEKV REVYNLLSGH YFTHATPTLY
210 220 230 240 250
NSCLKNPQLA SCFLITPRED SIEGVYHMIN QAAIITKYSG GIGLNLHGIR
260 270 280 290 300
SKGSSLRSTG GRSNGIIPLI QVLNATKRYI NQGAERRPGS IAIFLEPWHM
310 320 330 340 350
EIFDFLELRK NTGPEEFRAR DIFTALWIND LFMERVKNNE EWSLFCPSQA
360 370 380 390 400
VGLSDVWGEE FNALYCKYEK TISRTVVPAQ KLWKAIIEAQ IETGTPYMCY
410 420 430 440 450
KDACNRLSNQ QHLGTIKSSN LCAEIVEYSS GEETSVCNLA SICLPMFVKD
460 470 480 490 500
GWFDFEAFRR VVKILTVNLN RVIDFNYYPV EEARRSNMRN RPIGIGVQGL
510 520 530 540 550
ADLFAILRLA FESDGARSLN QDIFEAMYYS AMEASCELAE KEGPFPSYEG
560 570 580 590 600
SPISKGIFHF ELAGRKASGN WDWEGLRERI RRHGVRNSLL IALMPTAGTS
610 620 630 640 650
QIFGNNEAFE PHASNIYTRR THAGEFQIVN QHLVNDLVRL GLWSYEMKNL
660 670 680 690 700
VIENEGSIQN ITSIPHEIRE IYKTAWEIKM KSVIDLAADR QVFVDQSQSL
710 720 730 740 750
NIFIAKPTYS QLTSMHFYGY HCGLKTGMYY LRTRPITSAI KFTVDKKLAE
760
KTLSSMNDTD DPCSMCSS
Length:768
Mass (Da):87,941
Last modified:June 1, 2002 - v1
Checksum:i3E6BF6826805E20D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590449 Genomic DNA. Translation: CAD25811.1.
RefSeqiNP_586207.1. NM_001042040.1.

Genome annotation databases

EnsemblFungiiCAD25811; CAD25811; CAD25811.
GeneIDi859856.
KEGGiecu:ECU10_0920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590449 Genomic DNA. Translation: CAD25811.1.
RefSeqiNP_586207.1. NM_001042040.1.

3D structure databases

ProteinModelPortaliQ8SR37.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284813.NP_586207.1.

Proteomic databases

PRIDEiQ8SR37.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAD25811; CAD25811; CAD25811.
GeneIDi859856.
KEGGiecu:ECU10_0920.

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU10_0920.

Phylogenomic databases

eggNOGiKOG1112. Eukaryota.
COG0209. LUCA.
HOGENOMiHOG000057035.
InParanoidiQ8SR37.
KOiK10807.
OMAiYELLWQM.
OrthoDBiEOG092C0QYB.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_ENCCU
AccessioniPrimary (citable) accession number: Q8SR37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: June 1, 2002
Last modified: October 5, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.