Q8SR13 (PRS6A_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
26S protease regulatory subunit 6A

Gene names

Name:	RPT5
Ordered Locus Names:	ECU10_1420

Organism

Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]

Taxonomic identifier

284813 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Unikaryonidae › Encephalitozoon › Encephalitozoon cuniculi

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a regulatory subunit of the 26S proteasome which degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH By similarity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Developmental stage	Expressed in late sporogonial stages.
Sequence similarities	Belongs to the AAA ATPase family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	26S protease regulatory subunit 6A		PRO_0000382905
Regions
	Nucleotide binding	189 – 196	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8SR13 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 7A9ED4721D9775BC	FASTA	401	44,870
	10 20 30 40 50 60 MSLEELEAMD QSNPGEFLER VREKIRMLNS ETRILQSRVN TIKHDISTKN ASIAENMERI 70 80 90 100 110 120 RLNKQLPYLV GNVVEVLDEH SGVVNASTRM SSYLPITGLI PNSELRPGDL VALHKDTNIV 130 140 150 160 170 180 FEKLPPDYDM KVGGMVLKSD AKPDETYEDI GGLERQIEEL NEAIVLSLTH PERFEKLNIK 190 200 210 220 230 240 PPKGVLMYGP PGTGKTLMAR ACASKTNATF LKLAGPQLVQ MYIGDGARLV RDAFALAKER 250 260 270 280 290 300 KPTIIFIDEI DAIGAKRSDS DQTGDREVQR TMLELLNQLD GFSSSEEVKI IAATNRVDIL 310 320 330 340 350 360 DPALLRSGRL DRKIEFPLPN TLGRKRILQI HARKMSVRDD VNFDELARST EGFNGAQCKA 370 380 390 400 VCVEAGMAAL RKEKTEISQN DFMDGIQEVL SRKKSKLLYF T

References

[1]

"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi." Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P. Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.

[2]

"Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages." Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C. Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590449 Genomic DNA. Translation: CAD25861.1.
RefSeq	NP_586257.1. NM_001042090.1.
3D structure databases
HSSP	HSSP built from PDB template 1YPW based on UniProtKB Q01853.
ProteinModelPortal	Q8SR13.
ModBase	Search...
Protein-protein interaction databases
STRING	6035.ECU10_1420.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	859907.
KEGG	ecu:ECU10_1420.
Phylogenomic databases
eggNOG	COG1222.
HOGENOM	HOG000225143.
KO	K03065.
OMA	GALMYGP.
Family and domain databases
InterPro	IPR005937. 26S_Psome_P45. IPR003593. AAA+_ATPase. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. [Graphical view]
Pfam	PF00004. AAA. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01242. 26Sp45. 1 hit.
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRS6A_ENCCU

Accession

Primary (citable) accession number: Q8SR13

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program