Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8SR11 (PSB1_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable proteasome subunit beta type-1

EC=3.4.25.1
Alternative name(s):
26S proteasome beta-type subunit PRE3
Multicatalytic endopeptidase complex subunit PRE3
Gene names
Name:PRE3
Ordered Locus Names:ECU10_1450
OrganismEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]
Taxonomic identifier284813 [NCBI]
Taxonomic lineageEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon

Protein attributes

Sequence length203 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity By similarity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Developmental stage

Expressed in late sporogonial stages.

Sequence similarities

Belongs to the peptidase T1B family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 1010Removed in mature form By similarity
PRO_0000391408
Chain11 – 203193Probable proteasome subunit beta type-1
PRO_0000382760

Sites

Active site111Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8SR11 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: DC82D2EAD817DA3E

FASTA20322,189
        10         20         30         40         50         60 
MMSNEKEMTG TTIIAIKYDD GVLIGADSRT SMGAYVSSRV TDKLTQITDK IFVCRSGSSA 

        70         80         90        100        110        120 
DTQMISSYLR MYLSMYSQLE DSIPQVQRAA ALASKIIYEN PSLLAGLIVA GYDDKPRVFN 

       130        140        150        160        170        180 
ISLGGSLTER DWAIGGSGSA FIYGYCDVNW RSGMSLEEGI RFVRNAVSCA INRDNASGGC 

       190        200 
IRMSAISRTG VQRYFYPGDK VLQ 

« Hide

References

[1]"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi."
Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P.
Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GB-M1.
[2]"Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages."
Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.
Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL590449 Genomic DNA. Translation: CAD25864.1.
RefSeqNP_586260.1. NM_001042093.1.

3D structure databases

ProteinModelPortalQ8SR11.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6035.ECU10_1450.

Protein family/group databases

MEROPST01.010.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID859910.
KEGGecu:ECU10_1450.

Organism-specific databases

EuPathDBMicrosporidiaDB:ECU10_1450.

Phylogenomic databases

eggNOGCOG0638.
HOGENOMHOG000091079.
KOK02738.
OMACIRMCVI.
OrthoDBEOG7XDBSV.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSPR00141. PROTEASOME.
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSB1_ENCCU
AccessionPrimary (citable) accession number: Q8SR11
Entry history
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries