Q8SR10 (SYQ_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Ordered Locus Names:

ECU10_1460

Organism

Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]

Taxonomic identifier

284813 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Unikaryonidae › Encephalitozoon › Encephalitozoon cuniculi

Protein attributes

Sequence length	697 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 697	697	Probable glutamine--tRNA ligase		PRO_0000388381
Regions
	Motif	204 – 214	11	"HIGH" region By similarity
	Motif	441 – 445	5	"KMSKS" region By similarity
Sites
	Binding site	444	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8SR10 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 045C9353A142AE04	FASTA	697	81,248
	10 20 30 40 50 60 MADLEKILER LGISPEKRCQ VIKKEQVVRN MEKIFLGRDL SNRLLYTLAC IAPKNADLGL 70 80 90 100 110 120 LADLVDARVI KHESMLKECL RYTEKKDVSM EEMTRFVKRN EVSSEDVRKF VAKMRSDRVA 130 140 150 160 170 180 KKDMVSKARK AMPCADFRVV VEEINKVPDD VDGGDEKRPL EGGWLEEGEI KKLPKPSEIP 190 200 210 220 230 240 QINEEIRQAH LRRTGGRVVT RFPPEPNGIL HIGHAKAINL NFEYAKKFGG YTYLRYDDTN 250 260 270 280 290 300 PKNEEAEYFD SIYEDVRWLG FEPYKVTASS DYFDKMTEFG FQLIRKGKAY VCHLSQDEIC 310 320 330 340 350 360 ERRRQYVSDG TNDRSHLSQY RDRPVSENLR LFQEMVDGKW EEGKACLRFK MDTDTKNPLM 370 380 390 400 410 420 LDLVGIRILD VVHPRKNVKY TVYPTYEFAL CVSDSLEDVT HSFCTREFYT RQESYNWLLV 430 440 450 460 470 480 QLEIYKPIQW EFSRLNISNT VLSKRKLLPL KKYGIELDDP RLFTIKGMRR RGFPPEAINQ 490 500 510 520 530 540 FCRSLGFTFA ETTVDVKKLE NFVRDNLNRT SRRIMCVKEP LKVTIMNSTP CSISIPDLPG 550 560 570 580 590 600 SSVVRDVPFT PVIYIEKSDF MEKGDKDFLR LTPEQPVGLY MLYPIRVVKV TPDGIVAERW 610 620 630 640 650 660 DGVPRKFIHW VSEDSVEVEM RMYSSLWTSF SPKDATYLEE MNKDSLKVFH GLCDKRISDA 670 680 690 RIEDRFQFQR IGYFCVDKDT TKENIVVNLT IPLKNIA

References

[1]

"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi." Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P. Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590449 Genomic DNA. Translation: CAD25865.1.
RefSeq	NP_586261.1. NM_001042094.1.
3D structure databases
ProteinModelPortal	Q8SR10.
ModBase	Search...
Protein-protein interaction databases
STRING	6035.ECU10_1460.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	859911.
KEGG	ecu:ECU10_1460.
Organism-specific databases
EuPathDB	MicrosporidiaDB:ECU10_1460.
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000259233.
KO	K01886.
OMA	KEVCRFQ.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_ENCCU

Accession

Primary (citable) accession number: Q8SR10

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	June 1, 2002
Last modified:	May 29, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program