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Q8SQV6 (SYIC_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable isoleucine--tRNA ligase, cytoplasmic

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Ordered Locus Names:ECU11_1100
OrganismEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]
Taxonomic identifier284813 [NCBI]
Taxonomic lineageEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon

Protein attributes

Sequence length1017 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10171017Probable isoleucine--tRNA ligase, cytoplasmic
PRO_0000388378

Regions

Motif45 – 5511"HIGH" region By similarity
Motif609 – 6135"KMSKS" region By similarity

Sites

Binding site6121ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8SQV6 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 21B076581AA563B4

FASTA1,017117,392
        10         20         30         40         50         60 
MYRKNANFVE CEEEVLEYWR RNKSFERSCE RSRGREKFTF YDGPPFATGL PHYGHLLSGT 

        70         80         90        100        110        120 
IKDTVTRFFY QQGYDVDRRF GWDCHGLPVE YEIDKKLGIS SRAEVLEMGI GKYNAECRGI 

       130        140        150        160        170        180 
VMKYSSEWEA VVERLGRWVS FRDGYRTMDM TFMESVWNIF KELFSRGLIY RGFRVMPFST 

       190        200        210        220        230        240 
ACSTPLSNFE SNQNYKDVSD PSVLIAFPLL KPLGGYMLSL VAWTTTPWTL PSNCGLAVNP 

       250        260        270        280        290        300 
GFLYGVFEHK EKFYLMHVDR IGEYFKDARI LQRVSGRELE GLEYEQPFDY FEEYRKKGFF 

       310        320        330        340        350        360 
RVLASGFVTD TDGTGVVHCA PGFGECDYNA FVEKGLIREN DLVPCPVDEN GRYTSEVRRY 

       370        380        390        400        410        420 
AGRYVKDCDK AILSDIRDKV LMNQRIVHKY PFCWRSDTPL LYKLVPNWFV KVKDHVDSLL 

       430        440        450        460        470        480 
RNNEKINWVP PDIKYKRFHN WLENARDWSI SRNRFWGTPI PLWVTEDYSD MICIGSVGEL 

       490        500        510        520        530        540 
EELSGRKIDD IHREFIDGIV IHRNGREYRR VEEVLDCWFE SGSMPYAQDH WPFCKESGVD 

       550        560        570        580        590        600 
LGSLSVSGGE ERNKKLVKEN FPAHFIGEGL DQTRGWFYTL HVISSLLFGQ PAFLNVVVNG 

       610        620        630        640        650        660 
IVLAEDGRKM SKRLRNYPDP SHIFNTYGAD SLRMYLISSP VVEAENLKFS EGGVKEVLKT 

       670        680        690        700        710        720 
LIIPWYNSLG FYLENRDVEP DGRSLPMDGW ITASFDNFAW SLTRKMRKYE LSSVLTLALR 

       730        740        750        760        770        780 
FIDDLSNWYI RMYRKEIRAG HHAVLGEILK KFSIVMGPFT PFFSEYSYQS LNPGESVHFQ 

       790        800        810        820        830        840 
EYPVCKNGTH PFEMAKSIIA AVRRLRETNS ISLKTPLKSA TLMSSSSLYE GIKDYIDAIK 

       850        860        870        880        890        900 
TECNVLELLY KEEDRSMFDI TVKPNFLSLK KDKATMKKKM KVIQGLTGDQ AYSLLSSPLV 

       910        920        930        940        950        960 
VDGLEILRDD VLIVKKIRCE GIAQEFGDFS IIIDNTLDEG MVKMKIAREF HSYIQKLRKS 

       970        980        990       1000       1010 
AGLRVGDDVV VDIQCPDLKG IVSKYFDISF GSLGALSGRG EYEFDGTLYP VSLYKKL 

« Hide

References

[1]"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi."
Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P.
Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GB-M1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL590450 Genomic DNA. Translation: CAD26020.1.
RefSeqNP_586416.1. NM_001042249.1.

3D structure databases

ProteinModelPortalQ8SQV6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6035.ECU11_1100.

Proteomic databases

PRIDEQ8SQV6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID860069.
KEGGecu:ECU11_1100.

Organism-specific databases

EuPathDBMicrosporidiaDB:ECU11_1100.

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246403.
KOK01870.
OMANESINWI.
OrthoDBEOG7FNCGS.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYIC_ENCCU
AccessionPrimary (citable) accession number: Q8SQV6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries