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Protein

Pyruvate kinase

Gene

PYK1

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (ECU07_0800)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Pyruvate kinase (PYK1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34SubstrateBy similarity1
Metal bindingi36PotassiumBy similarity1
Metal bindingi38PotassiumBy similarity1
Metal bindingi68PotassiumBy similarity1
Metal bindingi69Potassium; via carbonyl oxygenBy similarity1
Sitei212Transition state stabilizerBy similarity1
Metal bindingi214MagnesiumSequence analysis1
Binding sitei237Substrate; via amide nitrogenBy similarity1
Metal bindingi238MagnesiumBy similarity1
Binding sitei238Substrate; via amide nitrogenBy similarity1
Binding sitei270SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:PYK1
Ordered Locus Names:ECU09_0640
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisMicrosporidiaUnikaryonidaeEncephalitozoon
Proteomesi

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU09_0640

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003794731 – 428Pyruvate kinaseAdd BLAST428

Proteomic databases

PRIDEiQ8SQP0

Expressioni

Developmental stagei

Expressed in late sporogonial stages.1 Publication

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi284813.XP_955618.1

Structurei

3D structure databases

SMRiQ8SQP0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiKOG2323 Eukaryota
COG0469 LUCA
HOGENOMiHOG000021559
InParanoidiQ8SQP0
KOiK00873
OrthoDBiEOG092C1RXZ

Family and domain databases

Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR015793 Pyrv_Knase_brl
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit

Sequencei

Sequence statusi: Complete.

Q8SQP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMVLTKIVC TIGPRTSSRE KIKELIDAGM SIARLNFSHG SREAHLEVIR
60 70 80 90 100
NIRDSRSGAG RHVSIALDTR GPEVRLRTPE MKDIKVEGGE VLRFSLLSSE
110 120 130 140 150
KDIWIPGVDL KSLGVDNRVF IDDGAIELRV VNVEEDGFEC EVLNSGMIKS
160 170 180 190 200
NKSMNFPGTD IGDRALGDED KNDIAFGLEN GIDMVFASFV SCRADVEEIR
210 220 230 240 250
RLVGSKVPVV SKIESCLGMR NLKEIALCSD GVMIARGDLG VEIGLENMFS
260 270 280 290 300
AQKRILYEVK REGRPVICAT QMMESMTLKN APNRSEISDV GNAVLDGCDC
310 320 330 340 350
VMLSAESAVG MFPVETVKFM RSICADAERY DMESRKGAGA CGVSSYVDGV
360 370 380 390 400
VICSGTESQI EKIYLSKPET PIIVISESLW ILRRFSIYRG IIPVYGKGSE
410 420
DAEATLRRLG LRGRFLAVGR EDVRMVSV
Length:428
Mass (Da):47,063
Last modified:May 29, 2013 - v2
Checksum:i7594602E9E920AC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590451 Genomic DNA Translation: CAD27037.2
RefSeqiXP_955618.1, XM_950525.1

Genome annotation databases

EnsemblFungiiCAD27037; CAD27037; CAD27037
GeneIDi860403
KEGGiecu:ECU09_0640

Similar proteinsi

Entry informationi

Entry nameiKPYK_ENCCU
AccessioniPrimary (citable) accession number: Q8SQP0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: May 29, 2013
Last modified: May 23, 2018
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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