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Q8SQP0

- KPYK_ENCCU

UniProt

Q8SQP0 - KPYK_ENCCU

Protein

Pyruvate kinase

Gene

PYK1

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 2 (29 May 2013)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.By similarity
    Potassium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei34 – 341SubstrateBy similarity
    Metal bindingi36 – 361PotassiumBy similarity
    Metal bindingi38 – 381PotassiumBy similarity
    Metal bindingi68 – 681PotassiumBy similarity
    Metal bindingi69 – 691Potassium; via carbonyl oxygenBy similarity
    Sitei212 – 2121Transition state stabilizerBy similarity
    Metal bindingi214 – 2141MagnesiumSequence Analysis
    Binding sitei237 – 2371Substrate; via amide nitrogenBy similarity
    Metal bindingi238 – 2381MagnesiumBy similarity
    Binding sitei238 – 2381Substrate; via amide nitrogenBy similarity
    Binding sitei270 – 2701SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:PYK1
    Ordered Locus Names:ECU09_0640
    OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
    Taxonomic identifieri284813 [NCBI]
    Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
    ProteomesiUP000000819: Chromosome IX

    Organism-specific databases

    EuPathDBiMicrosporidiaDB:ECU09_0640.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 428428Pyruvate kinasePRO_0000379473Add
    BLAST

    Expressioni

    Developmental stagei

    Expressed in late sporogonial stages.1 Publication

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi6035.ECU09_0640.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021559.
    KOiK00873.
    OrthoDBiEOG7M6DJC.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8SQP0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRMVLTKIVC TIGPRTSSRE KIKELIDAGM SIARLNFSHG SREAHLEVIR    50
    NIRDSRSGAG RHVSIALDTR GPEVRLRTPE MKDIKVEGGE VLRFSLLSSE 100
    KDIWIPGVDL KSLGVDNRVF IDDGAIELRV VNVEEDGFEC EVLNSGMIKS 150
    NKSMNFPGTD IGDRALGDED KNDIAFGLEN GIDMVFASFV SCRADVEEIR 200
    RLVGSKVPVV SKIESCLGMR NLKEIALCSD GVMIARGDLG VEIGLENMFS 250
    AQKRILYEVK REGRPVICAT QMMESMTLKN APNRSEISDV GNAVLDGCDC 300
    VMLSAESAVG MFPVETVKFM RSICADAERY DMESRKGAGA CGVSSYVDGV 350
    VICSGTESQI EKIYLSKPET PIIVISESLW ILRRFSIYRG IIPVYGKGSE 400
    DAEATLRRLG LRGRFLAVGR EDVRMVSV 428
    Length:428
    Mass (Da):47,063
    Last modified:May 29, 2013 - v2
    Checksum:i7594602E9E920AC5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590451 Genomic DNA. Translation: CAD27037.2.
    RefSeqiXP_955618.1. XM_950525.1.

    Genome annotation databases

    GeneIDi860403.
    KEGGiecu:ECU09_0640.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590451 Genomic DNA. Translation: CAD27037.2 .
    RefSeqi XP_955618.1. XM_950525.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6035.ECU09_0640.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 860403.
    KEGGi ecu:ECU09_0640.

    Organism-specific databases

    EuPathDBi MicrosporidiaDB:ECU09_0640.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021559.
    KOi K00873.
    OrthoDBi EOG7M6DJC.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GB-M1.
    2. "Identification of transcriptional signals in Encephalitozoon cuniculi widespread among Microsporidia phylum: support for accurate structural genome annotation."
      Peyretaillade E., Goncalves O., Terrat S., Dugat-Bony E., Wincker P., Cornman R.S., Evans J.D., Delbac F., Peyret P.
      BMC Genomics 10:607-607(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: GB-M1.
    3. "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages."
      Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.
      Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiKPYK_ENCCU
    AccessioniPrimary (citable) accession number: Q8SQP0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 7, 2009
    Last sequence update: May 29, 2013
    Last modified: October 1, 2014
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3