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Q8SQP0 (KPYK_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:PYK1
Ordered Locus Names:ECU09_0640
OrganismEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]
Taxonomic identifier284813 [NCBI]
Taxonomic lineageEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Developmental stage

Expressed in late sporogonial stages.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 428428Pyruvate kinase
PRO_0000379473

Sites

Metal binding361Potassium By similarity
Metal binding381Potassium By similarity
Metal binding681Potassium By similarity
Metal binding691Potassium; via carbonyl oxygen By similarity
Metal binding2141Magnesium Potential
Metal binding2381Magnesium By similarity
Binding site341Substrate By similarity
Binding site2371Substrate; via amide nitrogen By similarity
Binding site2381Substrate; via amide nitrogen By similarity
Binding site2701Substrate By similarity
Site2121Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8SQP0 [UniParc].

Last modified May 29, 2013. Version 2.
Checksum: 7594602E9E920AC5

FASTA42847,063
        10         20         30         40         50         60 
MRMVLTKIVC TIGPRTSSRE KIKELIDAGM SIARLNFSHG SREAHLEVIR NIRDSRSGAG 

        70         80         90        100        110        120 
RHVSIALDTR GPEVRLRTPE MKDIKVEGGE VLRFSLLSSE KDIWIPGVDL KSLGVDNRVF 

       130        140        150        160        170        180 
IDDGAIELRV VNVEEDGFEC EVLNSGMIKS NKSMNFPGTD IGDRALGDED KNDIAFGLEN 

       190        200        210        220        230        240 
GIDMVFASFV SCRADVEEIR RLVGSKVPVV SKIESCLGMR NLKEIALCSD GVMIARGDLG 

       250        260        270        280        290        300 
VEIGLENMFS AQKRILYEVK REGRPVICAT QMMESMTLKN APNRSEISDV GNAVLDGCDC 

       310        320        330        340        350        360 
VMLSAESAVG MFPVETVKFM RSICADAERY DMESRKGAGA CGVSSYVDGV VICSGTESQI 

       370        380        390        400        410        420 
EKIYLSKPET PIIVISESLW ILRRFSIYRG IIPVYGKGSE DAEATLRRLG LRGRFLAVGR 


EDVRMVSV 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi."
Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P.
Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GB-M1.
[2]"Identification of transcriptional signals in Encephalitozoon cuniculi widespread among Microsporidia phylum: support for accurate structural genome annotation."
Peyretaillade E., Goncalves O., Terrat S., Dugat-Bony E., Wincker P., Cornman R.S., Evans J.D., Delbac F., Peyret P.
BMC Genomics 10:607-607(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: GB-M1.
[3]"Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages."
Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.
Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL590451 Genomic DNA. Translation: CAD27037.2.
RefSeqXP_955618.1. XM_950525.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6035.ECU09_0640.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID860403.
KEGGecu:ECU09_0640.

Organism-specific databases

EuPathDBMicrosporidiaDB:ECU09_0640.

Phylogenomic databases

eggNOGCOG0469.
HOGENOMHOG000021559.
KOK00873.
OrthoDBEOG7M6DJC.

Enzyme and pathway databases

UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
ProtoNetSearch...

Entry information

Entry nameKPYK_ENCCU
AccessionPrimary (citable) accession number: Q8SQP0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: May 29, 2013
Last modified: July 9, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways