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Q8SQN7 (PSB2_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable proteasome subunit beta type-2

EC=3.4.25.1
Alternative name(s):
26S proteasome beta-type subunit PUP1
Multicatalytic endopeptidase complex subunit PUP1
Gene names
Name:PUP1
Ordered Locus Names:ECU09_0720
OrganismEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]
Taxonomic identifier284813 [NCBI]
Taxonomic lineageEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity By similarity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Developmental stage

Expressed in late sporogonial stages.

Sequence similarities

Belongs to the peptidase T1B family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 66Removed in mature form By similarity
PRO_0000391409
Chain7 – 227221Probable proteasome subunit beta type-2
PRO_0000382761

Sites

Active site71Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8SQN7 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: D7069F03AB8BAF53

FASTA22724,766
        10         20         30         40         50         60 
MITKTGTTIV GMKYKTGVIL AADTRSTQGP VVSDKNCVKI HQITDKIMCC GAGTAADASR 

        70         80         90        100        110        120 
VARMASRELR LFQNKYLRLP LVSHFRKVCT QHLHKYGGGI GAALIVGGID SEGCHLYEIH 

       130        140        150        160        170        180 
PHGSENSALF VSLGSGSLGA IATLESRYRA MDKDEAIDLA CDAVKAGILN DLYSGSNIDV 

       190        200        210        220 
CVIDYSGVEF LRNYRRIGVS ENTDTLVYPL DSVRIKREEV FDIVEEY 

« Hide

References

[1]"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi."
Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P.
Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GB-M1.
[2]"Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages."
Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.
Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL590451 Genomic DNA. Translation: CAD27045.1.
RefSeqXP_955626.1. XM_950533.1.

3D structure databases

ProteinModelPortalQ8SQN7.
SMRQ8SQN7. Positions 7-195.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6035.ECU09_0720.

Protein family/group databases

MEROPST01.011.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID860411.
KEGGecu:ECU09_0720.

Organism-specific databases

EuPathDBMicrosporidiaDB:ECU09_0720.

Phylogenomic databases

eggNOGCOG0638.
HOGENOMHOG000182856.
KOK02739.
OMAQIWCAGA.
OrthoDBEOG7TJ3V6.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSB2_ENCCU
AccessionPrimary (citable) accession number: Q8SQN7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries