Reviewed, UniProtKB/Swiss-Prot Q8SQI1 (3MG_ENCCU)

Names and origin

Protein names

Recommended name:
    Probable DNA-3-methyladenine glycosylase
    EC=3.2.2.21
Alternative name(s):
    3-methyladenine DNA glycosidase

Gene names

Ordered Locus Names:	ECU05_1590
AND
Ordered Locus Names:	ECU11_0140

Organism

Encephalitozoon cuniculi (Microsporidian parasite) [Complete proteome]

Taxonomic identifier

6035 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Microsporidia › Unikaryonidae › Encephalitozoon

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions By similarity.
Catalytic activity	Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.
Subcellular location	Nucleus Potential.
Developmental stage	Expressed in late sporogonial stages. Ref.2
Sequence similarities	Belongs to the DNA glycosylase MPG family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	base-excision repair Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro alkylbase DNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Probable DNA-3-methyladenine glycosylase		PRO_0000100069

Sequences

	Sequence		Length	Mass (Da)
	Q8SQI1-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: A4A22F1DAD5B443F	FASTA	208	23,773
	10 20 30 40 50 60 MDMGIPCSQL ARRLLGKMLC RRIEGRTTKG MIVETEAYLG KEDKACHSYG GRRTERNSAM 70 80 90 100 110 120 YMKAGTCYVY RIYGRYECFN ISSVEAGAGV LVRALEPLCG VSEMRERRGG RVKDRDIANG 130 140 150 160 170 180 PSKLCIAMGI TRREIDKEWI AGSEKIWLEE GREVADPEIV AGRRIGIRNC GEWEEKKLRF 190 200 YIRDNEFVSC IRRRELGNRK HGSVQQLP

References

[1]

"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi." Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P. Nature 414:450-453(2001) [PubMed: 11719806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.

[2]

"Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages." Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C. Proteomics 6:3625-3635(2006) [PubMed: 16691553] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL590445 Genomic DNA. Translation: CAD26679.1. AL590450 Genomic DNA. Translation: CAD25924.1.
RefSeq	NP_586320.1. NP_597502.1.
3D structure databases
HSSP	HSSP built from PDB template 1EWN based on UniProtKB P29372.
ModBase	Search...
Genome annotation databases
GeneID	859169. 859971.
GenomeReviews	Gene locus ECU05_1590 in contig AL590445_GR. Gene locus ECU11_0140 in contig AL590450_GR.
KEGG	ecu:ECU05_1590. ecu:ECU11_0140.
Phylogenomic databases
HOGENOM	Q8SQI1.
OMA	YHCFNVI.
Enzyme and pathway databases
BRENDA	3.2.2.21. 276319.
Family and domain databases
InterPro	IPR003180. PurDNA_glycsylse. [Graphical view]
Gene3D	G3DSA:3.10.300.10. PurDNA_glycsylse. 1 hit.
PANTHER	PTHR10429. PurDNA_glycsylse. 1 hit.
Pfam	PF02245. Pur_DNA_glyco. 1 hit. [Graphical view]
ProDom	PD009649. PurDNA_glycsylse. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00567. 3mg. 1 hit.
ProtoNet	Search...

Entry information

Entry name

3MG_ENCCU

Accession

Primary (citable) accession number: Q8SQI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)