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Protein

Na(+)/H(+) exchange regulatory cofactor NHE-RF2

Gene

SLC9A3R2

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus (By similarity).By similarity

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)/H(+) exchange regulatory cofactor NHE-RF2
Alternative name(s):
PDZ domain-containing protein NHERF-2
Sodium-hydrogen exchanger regulatory factor 2
Solute carrier family 9 isoform A3 regulatory factor 2
Gene namesi
Name:SLC9A3R2
Synonyms:NHERF2
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316Na(+)/H(+) exchange regulatory cofactor NHE-RF2PRO_0000096807Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei130 – 1301PhosphoserineBy similarity
Modified residuei183 – 1831PhosphoserineBy similarity
Modified residuei254 – 2541PhosphoserineBy similarity
Modified residuei282 – 2821PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Detected in kidney glomeruli.1 Publication

Interactioni

Subunit structurei

Homodimer, and heterodimer with SLC9A3R1. Binds ADRB2, SLC9A3, P2RY1, P2YR2, SRY, RDX, PDZK1 and LPAR2 (By similarity). Found in a complex with EZR, PODXL and SLC9A3R2 (By similarity). Interacts (via the PDZ domains) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is detected in glomerular epithelium cells (By similarity). Binds PODXL. Interacts with SGK1 and KCNJ1/ROMK1 (By similarity). Interacts (via the PDZ domains) with SLC26A6 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ATP2B2Q01814-13EBI-1174758,EBI-1174262From a different organism.
PODXLQ286457EBI-1174758,EBI-8375591

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ8SQG9. 2 interactions.
MINTiMINT-1773397.

Structurei

3D structure databases

ProteinModelPortaliQ8SQG9.
SMRiQ8SQG9. Positions 9-91, 147-232.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 9181PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini151 – 23181PDZ 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 PDZ (DHR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG052616.
InParanoidiQ8SQG9.
KOiK13358.

Family and domain databases

Gene3Di2.30.42.10. 2 hits.
InterProiIPR015098. EBP50_C-term.
IPR017300. NHERF-1/NHERF-2.
IPR031197. NHERF-2.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR14191:SF4. PTHR14191:SF4. 1 hit.
PfamiPF09007. EBP50_C. 2 hits.
PF00595. PDZ. 2 hits.
[Graphical view]
PIRSFiPIRSF037866. EBP50. 1 hit.
ProDomiPD283022. EBP50_C-term. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00228. PDZ. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiPS50106. PDZ. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8SQG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPEPLRPR LCRLVRGEQG YGFHLHGEKG RRGQFIRRVE PGSPAEAAAL
60 70 80 90 100
RAGDRLVEVN GVNVEGETHH QVVQRIKAVE GETRLLVVDK ETDEELRRRQ
110 120 130 140 150
LTCTEDMAQR GLPPAHDPWE PKPDWARAGS LSSDAGQKDV NGPPRELRPR
160 170 180 190 200
LCHLRKGPQG YGFNLHSDKS RPGQYIRSVD PGSPAAHSGL CAQDRLIEVN
210 220 230 240 250
GQNVEGLRHA EVVARIKAKE DEARLLLVDP ETDEYFKRLR VTPTEEHVEG
260 270 280 290 300
PLPSPITNGT SPAQDASAWK RDPFQESGLH LSPTAAEAKE KARATRVNKR
310
APQMDWNRKR EIFSNF
Length:316
Mass (Da):35,320
Last modified:June 1, 2002 - v1
Checksum:iF496FAD64156BCED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF358433 mRNA. Translation: AAL78310.1.
RefSeqiNP_001075576.1. NM_001082107.1.
UniGeneiOcu.2619.

Genome annotation databases

GeneIDi100008813.
KEGGiocu:100008813.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF358433 mRNA. Translation: AAL78310.1.
RefSeqiNP_001075576.1. NM_001082107.1.
UniGeneiOcu.2619.

3D structure databases

ProteinModelPortaliQ8SQG9.
SMRiQ8SQG9. Positions 9-91, 147-232.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8SQG9. 2 interactions.
MINTiMINT-1773397.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100008813.
KEGGiocu:100008813.

Organism-specific databases

CTDi9351.

Phylogenomic databases

HOVERGENiHBG052616.
InParanoidiQ8SQG9.
KOiK13358.

Family and domain databases

Gene3Di2.30.42.10. 2 hits.
InterProiIPR015098. EBP50_C-term.
IPR017300. NHERF-1/NHERF-2.
IPR031197. NHERF-2.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR14191:SF4. PTHR14191:SF4. 1 hit.
PfamiPF09007. EBP50_C. 2 hits.
PF00595. PDZ. 2 hits.
[Graphical view]
PIRSFiPIRSF037866. EBP50. 1 hit.
ProDomiPD283022. EBP50_C-term. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00228. PDZ. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiPS50106. PDZ. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "PDZ domain-mediated interaction of rabbit podocalyxin and Na(+)/H(+) exchange regulatory factor-2."
    Li Y., Li J., Straight S.W., Kershaw D.B.
    Am. J. Physiol. 282:F1129-F1139(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH PODXL.
    Tissue: Renal glomerulus.
  2. "Oligomerization of NHERF-1 and NHERF-2 PDZ domains: differential regulation by association with receptor carboxyl-termini and by phosphorylation."
    Lau A.G., Hall R.A.
    Biochemistry 40:8572-8580(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DIMERIZATION.

Entry informationi

Entry nameiNHRF2_RABIT
AccessioniPrimary (citable) accession number: Q8SQG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: June 1, 2002
Last modified: July 6, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.