Q8SQG8 (HYAL2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hyaluronidase-2
Short name=Hyal-2
EC=3.2.1.35

Alternative name(s):
Hyaluronoglucosaminidase-2

Gene names

Name:

HYAL2

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R By similarity.
Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.
Subunit structure	Interacts with MST1R By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	EGF-like domain Signal
Molecular function	Glycosidase Hydrolase Receptor
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cartilage development Inferred from sequence or structural similarity. Source: UniProtKB cellular response to UV-B Inferred from sequence or structural similarity. Source: UniProtKB cellular response to fibroblast growth factor stimulus Inferred from sequence or structural similarity. Source: UniProtKB cellular response to interleukin-1 Inferred from sequence or structural similarity. Source: UniProtKB cellular response to transforming growth factor beta stimulus Inferred from sequence or structural similarity. Source: UniProtKB cellular response to tumor necrosis factor Inferred from electronic annotation. Source: Compara defense response to virus Inferred from electronic annotation. Source: Compara hematopoietic progenitor cell differentiation Inferred from electronic annotation. Source: Compara hyaluronan catabolic process Inferred from sequence or structural similarity. Source: UniProtKB monocyte activation Inferred from sequence or structural similarity. Source: UniProtKB multicellular organismal aging Inferred from electronic annotation. Source: Compara multicellular organismal iron ion homeostasis Inferred from electronic annotation. Source: Compara negative regulation of MAP kinase activity Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell growth Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of fibroblast migration Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of protein kinase B signaling cascade Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of inflammatory response Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of interleukin-6 secretion Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of interleukin-8 secretion Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein import into nucleus Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara positive regulation of urine volume Inferred from sequence or structural similarity. Source: UniProtKB renal water absorption Inferred from sequence or structural similarity. Source: UniProtKB response to antibiotic Inferred from electronic annotation. Source: Compara response to reactive oxygen species Inferred from sequence or structural similarity. Source: UniProtKB skeletal system morphogenesis Inferred from electronic annotation. Source: Compara transformation of host cell by virus Inferred from sequence or structural similarity. Source: UniProtKB viral entry into host cell via membrane fusion with the plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	anchored to external side of plasma membrane Inferred from electronic annotation. Source: Compara anchored to plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB apical plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB cytosol Inferred from sequence or structural similarity. Source: UniProtKB endocytic vesicle Inferred from sequence or structural similarity. Source: UniProtKB lysosome Inferred from sequence or structural similarity. Source: UniProtKB membrane raft Inferred from sequence or structural similarity. Source: UniProtKB microvillus Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	hyaluronic acid binding Inferred from sequence or structural similarity. Source: UniProtKB hyaluronoglucuronidase activity Inferred from sequence or structural similarity. Source: UniProtKB hyalurononglucosaminidase activity Inferred from sequence or structural similarity. Source: UniProtKB receptor signaling protein tyrosine kinase inhibitor activity Inferred from sequence or structural similarity. Source: UniProtKB viral receptor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 451	431	Hyaluronidase-2		PRO_0000296276
	Propeptide	452 – 473	22	Removed in mature form Potential		PRO_0000296277
Regions
	Domain	364 – 442	79	EGF-like
Sites
	Active site	138	1	Proton donor By similarity
Amino acid modifications
	Lipidation	451	1	GPI-anchor amidated glycine Potential
	Glycosylation	77	1	N-linked (GlcNAc...) Potential
	Glycosylation	106	1	N-linked (GlcNAc...) Potential
	Glycosylation	340	1	N-linked (GlcNAc...) Potential
	Glycosylation	360	1	N-linked (GlcNAc...) Potential
	Disulfide bond	47 ↔ 343		By similarity
	Disulfide bond	214 ↔ 230		By similarity
	Disulfide bond	368 ↔ 379		By similarity
	Disulfide bond	373 ↔ 430		By similarity
	Disulfide bond	432 ↔ 441		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8SQG8 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: BC3D1E5DD120622E	FASTA	473	53,881
	10 20 30 40 50 60 MWTGLGPAVT LALVLVVAWA TELKPTAPPI FTGRPFVVAW DVPTQDCGPR HKMPLDPKDM 70 80 90 100 110 120 KAFDVQASPN EGFVNQNITI FYRDRLGMYP HFNSVGRSVH GGVPQNGSLW VHLEMLKGHV 130 140 150 160 170 180 EHYIRTQEPA GLAVIDWEDW RPVWVRNWQD KDVYRRLSRH LVAIRHPDWP PERVAKEAQY 190 200 210 220 230 240 EFEFAARQFM LETLRFVKAF RPRHLWGFYL FPDCYNHDYV QNWETYTGRC PDVEVSRNDQ 250 260 270 280 290 300 LAWLWAESTA LFPSVYLEET LASSTHGRNF VSFRVQEALR VADVHHANHA LPVYVFTRPT 310 320 330 340 350 360 YSRGLTGLSE MDLISTIGES AALGAAGVIL WGDAGFTTSN ETCRRLKDYL TRSLVPYVVN 370 380 390 400 410 420 VSWAAQYCSW AQCHGHGRCV RRDPNAHTFL HLSASSFRLV PSHAPDEPRL RPEGELSWAD 430 440 450 460 470 RNHLQMHFRC QCYLGWGGEQ CQWDRRRAAG GASGAWAGSH LTGLLAVAVL AFT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mechanism of cell entry and transformation by enzootic nasal tumor virus." Dirks C., Duh F.-M., Rai S.K., Lerman M.I., Miller A.D. J. Virol. 76:2141-2149(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF411973 mRNA. Translation: AAL78385.1. BT021810 mRNA. Translation: AAX46657.1. BT026155 mRNA. Translation: ABG66994.1. BC102042 mRNA. Translation: AAI02043.1.
IPI	IPI00712892.
RefSeq	NP_776772.1. NM_174347.2.
UniGene	Bt.29683.
3D structure databases
ProteinModelPortal	Q8SQG8.
ModBase	Search...
Protein family/group databases
CAZy	GH56. Glycoside Hydrolase Family 56.
Proteomic databases
PRIDE	Q8SQG8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000000612; ENSBTAP00000000612; ENSBTAG00000000484. ENSBTAT00000052079; ENSBTAP00000051198; ENSBTAG00000000484.
GeneID	281838.
KEGG	bta:281838.
Organism-specific databases
CTD	8692.
Phylogenomic databases
eggNOG	NOG77606.
GeneTree	ENSGT00550000074476.
HOGENOM	HOG000015133.
HOVERGEN	HBG052053.
InParanoid	Q8SQG8.
KO	K01197.
OMA	GWGGEQC.
OrthoDB	EOG4H463X.
Enzyme and pathway databases
BioCyc	CATTLE:281838-MONOMER.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR000742. EG-like_dom. IPR017853. Glycoside_hydrolase_SF. IPR018155. Hyaluronidase. [Graphical view]
PANTHER	PTHR11769. PTHR11769. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PIRSF	PIRSF038193. Hyaluronidase. 1 hit.
PRINTS	PR00846. GLHYDRLASE56.
SMART	SM00181. EGF. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20805744.

Entry information

Entry name

HYAL2_BOVIN

Accession

Primary (citable) accession number: Q8SQG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	June 1, 2002
Last modified:	May 29, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program