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Q8SQG7

- HYAL2_SHEEP

UniProt

Q8SQG7 - HYAL2_SHEEP

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Protein

Hyaluronidase-2

Gene

HYAL2

Organism
Ovis aries (Sheep)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R (By similarity).By similarity

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei138 – 1381Proton donorBy similarity

GO - Molecular functioni

  1. hyaluronic acid binding Source: UniProtKB
  2. hyaluronoglucuronidase activity Source: UniProtKB
  3. hyalurononglucosaminidase activity Source: UniProtKB
  4. receptor signaling protein tyrosine kinase inhibitor activity Source: UniProtKB
  5. virus receptor activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cartilage development Source: UniProtKB
  3. cellular response to fibroblast growth factor stimulus Source: UniProtKB
  4. cellular response to interleukin-1 Source: UniProtKB
  5. cellular response to transforming growth factor beta stimulus Source: UniProtKB
  6. cellular response to tumor necrosis factor Source: Ensembl
  7. cellular response to UV-B Source: UniProtKB
  8. defense response to virus Source: Ensembl
  9. fusion of virus membrane with host plasma membrane Source: UniProtKB
  10. glycosaminoglycan catabolic process Source: UniProtKB
  11. hematopoietic progenitor cell differentiation Source: Ensembl
  12. hyaluronan catabolic process Source: UniProtKB
  13. monocyte activation Source: UniProtKB
  14. multicellular organismal aging Source: Ensembl
  15. multicellular organismal iron ion homeostasis Source: Ensembl
  16. negative regulation of cell growth Source: UniProtKB
  17. negative regulation of fibroblast migration Source: UniProtKB
  18. negative regulation of MAP kinase activity Source: UniProtKB
  19. negative regulation of protein kinase B signaling Source: UniProtKB
  20. negative regulation of protein tyrosine kinase activity Source: UniProtKB
  21. positive regulation of extrinsic apoptotic signaling pathway Source: Ensembl
  22. positive regulation of inflammatory response Source: UniProtKB
  23. positive regulation of interleukin-6 secretion Source: UniProtKB
  24. positive regulation of interleukin-8 secretion Source: UniProtKB
  25. positive regulation of protein import into nucleus Source: Ensembl
  26. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  27. positive regulation of urine volume Source: UniProtKB
  28. renal water absorption Source: UniProtKB
  29. response to antibiotic Source: Ensembl
  30. response to reactive oxygen species Source: UniProtKB
  31. response to virus Source: UniProtKB
  32. skeletal system morphogenesis Source: Ensembl
  33. transformation of host cell by virus Source: UniProtKB
  34. viral entry into host cell Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Receptor

Keywords - Biological processi

Host-virus interaction

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase-2 (EC:3.2.1.35)
Short name:
Hyal-2
Alternative name(s):
Hyaluronoglucosaminidase-2
Gene namesi
Name:HYAL2
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
ProteomesiUP000002356: Chromosome 19

Subcellular locationi

Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

GO - Cellular componenti

  1. anchored component of external side of plasma membrane Source: Ensembl
  2. anchored component of plasma membrane Source: UniProtKB
  3. apical plasma membrane Source: UniProtKB
  4. cytoplasmic membrane-bounded vesicle Source: UniProtKB
  5. cytoplasmic vesicle Source: UniProtKB
  6. cytosol Source: UniProtKB
  7. endocytic vesicle Source: UniProtKB
  8. lysosome Source: UniProtKB
  9. membrane raft Source: UniProtKB
  10. microvillus Source: UniProtKB
  11. perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 451431Hyaluronidase-2PRO_0000239065Add
BLAST
Propeptidei452 – 47625Removed in mature formSequence AnalysisPRO_0000239066Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi47 ↔ 343By similarity
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi214 ↔ 230By similarity
Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi360 – 3601N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi368 ↔ 379By similarity
Disulfide bondi373 ↔ 430By similarity
Disulfide bondi432 ↔ 441By similarity
Lipidationi451 – 4511GPI-anchor amidated glycineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Interactioni

Subunit structurei

Interacts with MST1R (By similarity). Interacts with Jaagsiekte sheep retrovirus (JSRV) envelope proteins.By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8SQG7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini364 – 44279EGF-likeAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated
Contains 1 EGF-like domain.Curated

Keywords - Domaini

EGF-like domain, Signal

Phylogenomic databases

GeneTreeiENSGT00550000074476.
HOVERGENiHBG052053.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000742. EG-like_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SMARTiSM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8SQG7 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWTGLGPAVT LALVLVVAWA TELKPTAPPI FTGRPFVVAW DVPTQDCGPR
60 70 80 90 100
HKMPLDPKDM KAFDVQASPN EGFVNQNITI FYRDRLGMYP HFNSVGRSVH
110 120 130 140 150
GGVPQNGSLW VHLEMLKGHV EHYIRTQEPA GLAVIDWEDW RPVWVRNWQD
160 170 180 190 200
KDVYRRLSRQ LVASHHPDWP PERIVKEAQY EFEFAARQFM LETLRFVKAF
210 220 230 240 250
RPRHLWGFYL FPDCYNHDYV QNWETYTGRC PDVEVSRNDQ LSWLWAESTA
260 270 280 290 300
LFPSVYLEET LASSTHGRNF VSFRVQEALR VADVHHANHA LPVYVFTRPT
310 320 330 340 350
YSRGLTGLSE MDLISTIGES AALGAAGVIL WGDAGFTTSN ETCRRLKDYL
360 370 380 390 400
TRSLVPYVVN VSWAAQYCSW AQCHGHGRCV RRDPNAHTFL HLSASSFRLV
410 420 430 440 450
PSHAPDEPRL RPEGELSWAD RNHLQTHFRC QCYLGWGGEQ CQWDRRRAAG
460 470
GASGAWAGSH LTGLLAVAVL AFTWTS
Length:476
Mass (Da):54,229
Last modified:June 1, 2002 - v1
Checksum:i427C9277ACF7AAAA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF411974 mRNA. Translation: AAL78386.1.
RefSeqiNP_001009754.1. NM_001009754.1.
UniGeneiOar.905.

Genome annotation databases

EnsembliENSOART00000010462; ENSOARP00000010309; ENSOARG00000009614.
GeneIDi443154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF411974 mRNA. Translation: AAL78386.1 .
RefSeqi NP_001009754.1. NM_001009754.1.
UniGenei Oar.905.

3D structure databases

ProteinModelPortali Q8SQG7.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH56. Glycoside Hydrolase Family 56.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSOART00000010462 ; ENSOARP00000010309 ; ENSOARG00000009614 .
GeneIDi 443154.

Organism-specific databases

CTDi 8692.

Phylogenomic databases

GeneTreei ENSGT00550000074476.
HOVERGENi HBG052053.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR000742. EG-like_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view ]
PANTHERi PTHR11769. PTHR11769. 1 hit.
Pfami PF01630. Glyco_hydro_56. 1 hit.
[Graphical view ]
PIRSFi PIRSF038193. Hyaluronidase. 1 hit.
PRINTSi PR00846. GLHYDRLASE56.
SMARTi SM00181. EGF. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Mechanism of cell entry and transformation by enzootic nasal tumor virus."
    Dirks C., Duh F.-M., Rai S.K., Lerman M.I., Miller A.D.
    J. Virol. 76:2141-2149(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Candidate tumor suppressor HYAL2 is a glycosylphosphatidylinositol (GPI)-anchored cell-surface receptor for jaagsiekte sheep retrovirus, the envelope protein of which mediates oncogenic transformation."
    Rai S.K., Duh F.-M., Vigdorovich V., Danilkovitch-Miagkova A., Lerman M.I., Miller A.D.
    Proc. Natl. Acad. Sci. U.S.A. 98:4443-4448(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH JSRV ENVELOPE PROTEINS.

Entry informationi

Entry nameiHYAL2_SHEEP
AccessioniPrimary (citable) accession number: Q8SQG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 1, 2002
Last modified: October 1, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Acts as a receptor for the Jaagsiekte sheep retrovirus (JSRV), which induces ovine pulmonary adenocarcinoma. A possible mechanism is that binding to JSRV envelope proteins may liberate the oncogenic factor MST1R that is normally negatively regulated by HYAL2, leading to oncogenic transformation.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3