Reviewed, UniProtKB/Swiss-Prot Q8SQ20 (GGTA1_CALJA)

Names and origin

Protein names

Recommended name:
    N-acetyllactosaminide alpha-1,3-galactosyltransferase
    EC=2.4.1.87
Alternative name(s):
    UDP-galactose:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase
      Short name=Galactosyltransferase

Gene names

Name:

GGTA1

Organism

Callithrix jacchus (Common marmoset)

Taxonomic identifier

9483 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Platyrrhini › Cebidae › Callitrichinae › Callithrix

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transfer of galactose from UDP-galactose to an acceptor molecule (R).
Catalytic activity	UDP-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Domain	The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis By similarity.
Sequence similarities	Belongs to the glycosyltransferase 6 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N-acetyllactosaminide 3-alpha-galactosyltransferase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	N-acetyllactosaminide alpha-1,3-galactosyltransferase		PRO_0000333698
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Signal-anchor for type II membrane protein Potential
	Topological domain	28 – 376	349	Lumenal Potential
Sites
	Metal binding	233	1	Manganese By similarity
	Metal binding	235	1	Manganese By similarity
Amino acid modifications
	Glycosylation	301	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8SQ20-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: D1A8645E8E647D75	FASTA	376	44,439
	10 20 30 40 50 60 MNVKGKVILS MLVVSTVIVV FWEYINSPEG SFLWIYHSKN PEVDDSSAQK GWWFPGWFNN 70 80 90 100 110 120 GIHNYQQEEE DTDKEKGREE EQRKEDDTTE LRLWDWFNPK KRPEVVTVTK WKAPVVWEGT 130 140 150 160 170 180 YNKAILENYY AKQKITVGLT VFAIGRYIEH YLEEFVTSAN RYFMVGHKVI FYVMVDDVSK 190 200 210 220 230 240 VPFIELGPLR SFKVFEVKPE KRWQDISMMR MKTIGEHILA HIQHEVDFLF CMDVDQVFQD 250 260 270 280 290 300 HFGVETLGQS VAQLQAWWYK ADPDDFTYER RKESAAYIPF GQGDFYYHAA IFGGTPIQVL 310 320 330 340 350 360 NITQECFKGI LLDKKNDIEA EWHDESHLNE YFLLNKPSKI LSPEYCWDYH IGLPSDIKTV 370 KLSWQTKEYN LVRNKV

References

[1]

"Molecular basis of evolutionary loss of the alpha 1,3-galactosyltransferase gene in higher primates." Koike C., Fung J.J., Geller D.A., Kannagi R., Libert T., Luppi P., Nakashima I., Profozich J., Rudert W., Sharma S.B., Starzl T.E., Trucco M. J. Biol. Chem. 277:10114-10120(2002) [PubMed: 11773054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF384428 mRNA. Translation: AAL93214.1.
3D structure databases
HSSP	HSSP built from PDB template 1O7Q based on UniProtKB P14769.
SMR	Q8SQ20. Positions 90-376.
ModBase	Search...
Protein family/group databases
CAZy	GT6. Glycosyltransferase Family 6.
Phylogenomic databases
HOVERGEN	Q8SQ20.
Family and domain databases
InterPro	IPR005076. Glyco_trans_6. [Graphical view]
PANTHER	PTHR10462. Glyco_trans_6. 1 hit.
Pfam	PF03414. Glyco_transf_6. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GGTA1_CALJA

Accession

Primary (citable) accession number: Q8SQ20

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)