Q8SQ12 (RNAS1_PONPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease pancreatic
EC=3.1.27.5

Alternative name(s):
RNase 1
RNase A

Gene names

Name:	RNASE1
Synonyms:	RNS1

Organism

Pongo pygmaeus (Bornean orangutan)

Taxonomic identifier

9600 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas and other tissues and body fluids (indicating it may have other physiological functions besides its role in digestion).
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	By similarity
	Chain	29 – 156	128	Ribonuclease pancreatic		PRO_0000030937
Regions
	Region	69 – 73	5	Substrate binding By similarity
Sites
	Active site	40	1	Proton acceptor By similarity
	Active site	147	1	Proton donor By similarity
	Binding site	35	1	Substrate By similarity
	Binding site	38	1	Substrate By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	113	1	Substrate By similarity
Amino acid modifications
	Glycosylation	50	1	N-linked (GlcNAc...) Potential
	Glycosylation	62	1	N-linked (GlcNAc...) Potential
	Glycosylation	104	1	N-linked (GlcNAc...) Potential
	Disulfide bond	54 ↔ 112		By similarity
	Disulfide bond	68 ↔ 123		By similarity
	Disulfide bond	86 ↔ 138		By similarity
	Disulfide bond	93 ↔ 100		By similarity
Experimental info
	Sequence conflict	129	1	T → P in CAH93057. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8SQ12 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: C87B07BF46B1F141	FASTA	156	17,608
	10 20 30 40 50 60 MALEKSLVLL PLLVLILLVL GWVQPSLGKE SRAKKFQRQH MDSGSSPNSN STYCNQMMRR 70 80 90 100 110 120 RNMTQGRCKP VNTFVHEPLV DVQNVCFQEK VTCKNGQGNC YKSNSSMHIT DCRLTHGSRY 130 140 150 PNCAYRTSTK ERHIIVACEG SPYVPVHFDA SVEDST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Adaptive evolution of a duplicated pancreatic ribonuclease gene in a leaf-eating monkey." Zhang J., Zhang Y.-P., Rosenberg H.F. Nat. Genet. 30:411-415(2002) [PubMed: 11925567] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Duplication and divergence of two distinct pancreatic ribnuclease genes in leaf-eating African and Asian colobine monkeys." Schienman J.E., Holt R.A., Auerbach M.R., Stewart C.B. Mol. Biol. Evol. 23:1465-1479(2006) [PubMed: 16751256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF449630 Genomic DNA. Translation: AAL87051.1. DQ494868 Genomic DNA. Translation: ABF00145.1. CR860954 mRNA. Translation: CAH93057.1.
UniGene	Pab.6304.
3D structure databases
ProteinModelPortal	Q8SQ12.
SMR	Q8SQ12. Positions 29-155.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8SQ12.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
GeneTree	ENSGT00390000007840.
HOVERGEN	HBG008396.
InParanoid	Q8SQ12.
Family and domain databases
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS1_PONPY

Accession

Primary (citable) accession number: Q8SQ12
Secondary accession number(s): Q1KHR1, Q5R5A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 1, 2002
Last modified:	July 27, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program