Reviewed, UniProtKB/Swiss-Prot Q8SPY7 (RNAS2_PAPHA)

Names and origin

Protein names

Recommended name:
    Non-secretory ribonuclease
    EC=3.1.27.5
Alternative name(s):
    Ribonuclease US
    Eosinophil-derived neurotoxin
    RNase UpI-2
    Ribonuclease 2
      Short name=RNase 2

Gene names

Name:	RNASE2
Synonyms:	EDN, RNS2

Organism

Papio hamadryas (Hamadryas baboon)

Taxonomic identifier

9557 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Papio

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Possesses a wide variety of biological activities By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subcellular location	Lysosome Probable. Cytoplasmic granule By similarity. Note: Matrix of eosinophil's large specific granule By similarity.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein Nitration
Gene Ontology (GO)
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	By similarity
	Chain	28 – 160	133	Non-secretory ribonuclease		PRO_0000030879
Regions
	Region	65 – 69	5	Substrate binding By similarity
Sites
	Active site	42	1	Proton acceptor By similarity
	Active site	155	1	Proton donor By similarity
Amino acid modifications
	Modified residue	60	1	Nitrated tyrosine By similarity
	Glycosylation	34	1	C-linked (Man) By similarity
	Glycosylation	44	1	N-linked (GlcNAc...) Potential
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	111	1	N-linked (GlcNAc...) Potential
	Glycosylation	138	1	N-linked (GlcNAc...) Potential
	Disulfide bond	50 ↔ 110		By similarity
	Disulfide bond	64 ↔ 122		By similarity
	Disulfide bond	82 ↔ 137		By similarity
	Disulfide bond	89 ↔ 98		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8SPY7-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: D3BCD9F336697473	FASTA	160	17,940
	10 20 30 40 50 60 MVPKLFTSPI CLLLLLGLMG VEGSLHAKPG QFTWAQWFEI QHINMTSGQC TNAMLVINNY 70 80 90 100 110 120 QRRCKNQNTF LLTTFADVVH VCGNPSMPCP SNTSLNNCHH SGVQVPLIHC NLTTPSRRIS 130 140 150 160 NCRYTQTTAN KYYIVACNNS DPVRDPPQYP VVPVHLDRVI

References

[1]	"Complementary advantageous substitutions in the evolution of an antiviral RNase of higher primates." Zhang J., Rosenberg H.F. Proc. Natl. Acad. Sci. U.S.A. 99:5486-5491(2002) [PubMed: 11917138] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF479629 Genomic DNA. Translation: AAM14436.1.
3D structure databases
HSSP	HSSP built from PDB template 1GQV based on UniProtKB P10153.
SMR	Q8SPY7. Positions 26-160.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q8SPY7.
Enzyme and pathway databases
BRENDA	3.1.27.5. 39388.
Family and domain databases
InterPro	IPR001427. RNaseA. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS2_PAPHA

Accession

Primary (citable) accession number: Q8SPY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	June 1, 2002
Last modified:	October 13, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)