Q8SPY3 (RNAS2_SAGLB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-secretory ribonuclease
EC=3.1.27.5

Alternative name(s):
Eosinophil-derived neurotoxin
RNase UpI-2
Ribonuclease 2
Short name=RNase 2
Ribonuclease US

Gene names

Name:	RNASE2
Synonyms:	EDN, RNS2

Organism

Saguinus labiatus (Red-chested mustached tamarin)

Taxonomic identifier

78454 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Platyrrhini › Cebidae › Callitrichinae › Saguinus

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Possesses a wide variety of biological activities By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Interacts with and forms a tight 1:1 complex with RNH1. Dimerization of two such complexes may occur By similarity.
Subcellular location	Lysosome Probable. Cytoplasmic granule By similarity. Note: Matrix of eosinophil's large specific granule By similarity.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Glycoprotein Nitration
Gene Ontology (GO)
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	By similarity
	Chain	28 – 158	131	Non-secretory ribonuclease		PRO_0000251796
Regions
	Region	65 – 69	5	Substrate binding By similarity
Sites
	Active site	42	1	Proton acceptor By similarity
	Active site	153	1	Proton donor By similarity
Amino acid modifications
	Modified residue	60	1	Nitrated tyrosine By similarity
	Glycosylation	34	1	C-linked (Man) By similarity
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	111	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8SPY3 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 7A0663BD45399E47	FASTA	158	17,853
	10 20 30 40 50 60 MVPKLFTSQI CLLLLLGLSS LEVSLHAKPQ QFSWAQWFRI QHIQTTPLHC TYAMRAINRY 70 80 90 100 110 120 QPRCKNRNTF LHTTFADVVN VCGNTNMTCP GNASLNNCHH SGVRVPLTYC NLTGPQTISN 130 140 150 CVYSSTQANM FYVVACDNRD PRDPPQYPVV PVHLDTII

References

[1]	"Complementary advantageous substitutions in the evolution of an antiviral RNase of higher primates." Zhang J., Rosenberg H.F. Proc. Natl. Acad. Sci. U.S.A. 99:5486-5491(2002) [PubMed: 11917138] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF479633 Genomic DNA. Translation: AAM14440.1.
3D structure databases
HSSP	HSSP built from PDB template 2BEX based on UniProtKB P10153.
ProteinModelPortal	Q8SPY3.
SMR	Q8SPY3. Positions 26-158.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008396.
Family and domain databases
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS2_SAGLB

Accession

Primary (citable) accession number: Q8SPY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	June 1, 2002
Last modified:	July 27, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program