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Protein

Neuronal acetylcholine receptor subunit alpha-5

Gene

CHRNA5

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-BTA-629597. Highly calcium permeable nicotinic acetylcholine receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-5
Gene namesi
Name:CHRNA5
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 21

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 261232ExtracellularBy similarityAdd
BLAST
Transmembranei262 – 28221HelicalSequence analysisAdd
BLAST
Transmembranei289 – 30921HelicalSequence analysisAdd
BLAST
Transmembranei324 – 34421HelicalSequence analysisAdd
BLAST
Topological domaini345 – 43793CytoplasmicBy similarityAdd
BLAST
Transmembranei438 – 45821HelicalSequence analysisAdd
BLAST
Topological domaini459 – 47517ExtracellularBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence analysisAdd
BLAST
Chaini30 – 475446Neuronal acetylcholine receptor subunit alpha-5PRO_0000244646Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi177 ↔ 191By similarity
Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence analysis
Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence analysis
Disulfide bondi241 ↔ 242Associated with receptor activationBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8SPU7.

Expressioni

Gene expression databases

ExpressionAtlasiQ8SPU7. baseline.

Interactioni

Subunit structurei

Neuronal AChR seems to be composed of two different type of subunits: alpha and non-alpha (beta).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000055853.

Structurei

3D structure databases

ProteinModelPortaliQ8SPU7.
SMRiQ8SPU7. Positions 257-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiQ8SPU7.
KOiK04807.
OMAiSIAKHED.
OrthoDBiEOG72JWGV.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 2 hits.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8SPU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPGWGRWV LGLGPLLLQV FLPFQLVAGR WGPEGAGGGV RRGLAEPSVV
60 70 80 90 100
AKHEDSLFKD LFQDYERWVR PVEHLNDRIK IKFGLAISQL VDVDEKNQLM
110 120 130 140 150
TTNVWLKQEW IDVKLRWNPD DYGGIKLIRV PSDSLWTPDI VLFDNADGRF
160 170 180 190 200
EGASTKTVVR YDGTVTWTPP ANYKSSCTID VTFFPFDLQN CSMKFGSWTY
210 220 230 240 250
DGSQVDIILE DQDVDKRDFF DNGEWEIVSA TGSKGNRTDS CCWYPYITYS
260 270 280 290 300
FVIKRLPLFY TLFLIIPCIG LSFLTVLVFY LPSNEGEKIC LCTSVLVSLT
310 320 330 340 350
VFLLVIEEII PSSSKVIPLI GEYLVFTMIF VTLSIMVTVF AINIHHRSSS
360 370 380 390 400
THDAMAPWVR KIFLHKLPKL LCMRSHVDRY FSQKEEARSS RGPRSSRNAL
410 420 430 440 450
EAALDSVRYI TRHVMKETDV REVVEDWKFI AQVLDRMFLW TFLLVSVVGS
460 470
LGLFVPVIYK WANIIVPIHI GNENK
Length:475
Mass (Da):54,260
Last modified:June 1, 2002 - v1
Checksum:i24658023DF0E3D1A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF487464 mRNA. Translation: AAL88711.1.
RefSeqiNP_776939.1. NM_174514.2.
UniGeneiBt.9979.

Genome annotation databases

EnsembliENSBTAT00000018151; ENSBTAP00000018151; ENSBTAG00000013657.
GeneIDi282177.
KEGGibta:282177.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF487464 mRNA. Translation: AAL88711.1.
RefSeqiNP_776939.1. NM_174514.2.
UniGeneiBt.9979.

3D structure databases

ProteinModelPortaliQ8SPU7.
SMRiQ8SPU7. Positions 257-353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000055853.

Proteomic databases

PaxDbiQ8SPU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000018151; ENSBTAP00000018151; ENSBTAG00000013657.
GeneIDi282177.
KEGGibta:282177.

Organism-specific databases

CTDi1138.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiQ8SPU7.
KOiK04807.
OMAiSIAKHED.
OrthoDBiEOG72JWGV.

Enzyme and pathway databases

ReactomeiR-BTA-629597. Highly calcium permeable nicotinic acetylcholine receptors.

Gene expression databases

ExpressionAtlasiQ8SPU7. baseline.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 2 hits.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Neuronal nicotinic acetylcholine receptors on bovine chromaffin cells: cloning, expression, and genomic organization of receptor subunits."
    Campos-Caro A., Smillie F.I., Dominguez del Toro E., Rovira J.C., Vicente-Agullo F., Chapuli J., Juiz J.M., Sala S., Sala F., Ballesta J.J., Criado M.
    J. Neurochem. 68:488-497(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiACHA5_BOVIN
AccessioniPrimary (citable) accession number: Q8SPU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 1, 2002
Last modified: June 8, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.