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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4

Gene

PLCD4

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca2+ from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca2+ mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation (By similarity).By similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. Two of the Ca2+ ions are bound to the C2 domain.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei312PROSITE-ProRule annotation1
Metal bindingi313Calcium 1; catalyticBy similarity1
Metal bindingi342Calcium 1; catalyticBy similarity1
Metal bindingi344Calcium 1; catalyticBy similarity1
Active sitei357PROSITE-ProRule annotation1
Metal bindingi391Calcium 1; catalyticBy similarity1
Binding sitei440SubstrateBy similarity1
Binding sitei442SubstrateBy similarity1
Binding sitei532SubstrateBy similarity1
Binding sitei559SubstrateBy similarity1
Metal bindingi660Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi662Calcium 2By similarity1
Metal bindingi686Calcium 2By similarity1
Metal bindingi715Calcium 3By similarity1
Metal bindingi716Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi717Calcium 3By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi154 – 1651PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi190 – 2012PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-SSC-1855204 Synthesis of IP3 and IP4 in the cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-delta-4
Phospholipase C-delta-4
Short name:
PLC-delta-4
Gene namesi
Name:PLCD4
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003068271 – 7721-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4Add BLAST772

Proteomic databases

PaxDbiQ8SPR7
PeptideAtlasiQ8SPR7
PRIDEiQ8SPR7

Expressioni

Gene expression databases

BgeeiENSSSCG00000016192
ExpressionAtlasiQ8SPR7 differential
GenevisibleiQ8SPR7 SS

Interactioni

Subunit structurei

Interacts with GRIP1.By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000017154

Structurei

3D structure databases

ProteinModelPortaliQ8SPR7
SMRiQ8SPR7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 131PHPROSITE-ProRule annotationAdd BLAST109
Domaini141 – 176EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini177 – 212EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini210 – 244EF-hand 3PROSITE-ProRule annotationAdd BLAST35
Domaini297 – 442PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST146
Domaini503 – 619PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini624 – 729C2PROSITE-ProRule annotationAdd BLAST106

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 60Substrate bindingBy similarityAdd BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi741 – 744PDZ-binding4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi448 – 483Glu-richAdd BLAST36
Compositional biasi489 – 496Poly-Lys8

Domaini

The PDZ-binding motif mediates the interaction with GRIP1.By similarity
The C2 domain mediates pre-localization to the membrane prior to Ca2+ import and non-selective Ca2+-mediated targeting to various cellular membranes.By similarity
The PH domain is not a critical determinant of the membrane localization.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0169 Eukaryota
ENOG410XPSW LUCA
HOGENOMiHOG000006871
HOVERGENiHBG053610
InParanoidiQ8SPR7
KOiK05857
OMAiQLCGQSS
OrthoDBiEOG091G02KH
TreeFamiTF313216

Family and domain databases

Gene3Di2.30.29.301 hit
2.60.40.1501 hit
3.20.20.1901 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR001192 PI-PLC_fam
IPR028387 PLC-delta4
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR015359 PLC_EF-hand-like
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 1 hit
PTHR10336:SF31 PTHR10336:SF31, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF09279 EF-hand_like, 1 hit
PF00169 PH, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00054 EFh, 3 hits
SM00233 PH, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF51695 SSF51695, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 3 hits
PS50003 PH_DOMAIN, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q8SPR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLLQGLSA SPGSGLPINQ DLLLMQKGML MRKVRSKSWK KLRYYRLQDD
60 70 80 90 100
GMTVWHARQA GGRAKPSFSI SDVETVRGGH ESELLRNLAE EFPLEQGFTI
110 120 130 140 150
VFHGRRSNLD LVANSVEEAQ VWMQGLQLLV DFVTNMDQQE RLDQWLSDWF
160 170 180 190 200
QRGDKNQDGR MSFGEVQRLL HLMNVEMDQE HAFQLFQTAD TSQSGTLEGE
210 220 230 240 250
EFVEFYKSLT KRAEVQELFE NFSSDGQKLT LLEFVDFLQE EQKEGERASD
260 270 280 290 300
LALELIDRHE PSDSGKLRHV LSLDGFLSYL CSKDGDIFNP TCLPIYQDMT
310 320 330 340 350
QPLNHYFINS SHNTYLVGDQ LCGQSSVEGY IRALKRGCRC VEVDIWDGPN
360 370 380 390 400
GEPVVYHGHT LTSRIPFKDV VAAIAQYAFQ TSDYPVILSL ENHCSWEQQE
410 420 430 440 450
MIAHHLTEIL GEQLLSTTLD GQLPTQLPSP EELRRKILVK GKKLRTLEED
460 470 480 490 500
LEEEEEEPEE SELEGEQEAE LELEAQFESE PQELSPRSKD KKKKVKAILC
510 520 530 540 550
PALSALVVYL KAVSFYSFAH SREHYRFYEI SSFSEAKAKS LIKESGNEFV
560 570 580 590 600
QHNTWQLSRV YPGGLRTDSS NYNPQEFWNA GCQMVAMNMQ TAGLEMDLCD
610 620 630 640 650
GLFRQNAGCG YVLKPDFLRD AQSSFHPERP ISPSKAQTLL IQVISGQQLP
660 670 680 690 700
KVDDSKEGSI VDPLVRVEIF GVRPDTARQE TSYVENNGFN PYWGQTLCFR
710 720 730 740 750
VLVPELALLR FVVKDYDWKS RNDFVGQYTL PWNCMQQGYR HIHLLSKDGI
760 770
SLHPASIFVH ICIREGIEGV ES
Length:772
Mass (Da):88,075
Last modified:June 1, 2002 - v1
Checksum:i86E5706A74247548
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF498759 mRNA Translation: AAM18122.1
RefSeqiNP_999217.1, NM_214052.1
UniGeneiSsc.191

Genome annotation databases

GeneIDi397119
KEGGissc:397119

Similar proteinsi

Entry informationi

Entry nameiPLCD4_PIG
AccessioniPrimary (citable) accession number: Q8SPR7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: June 1, 2002
Last modified: April 25, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome