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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4

Gene

PLCD4

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca2+ from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca2+ mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation (By similarity).By similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. Two of the Ca2+ ions are bound to the C2 domain.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei312PROSITE-ProRule annotation1
Metal bindingi313Calcium 1; catalyticBy similarity1
Metal bindingi342Calcium 1; catalyticBy similarity1
Metal bindingi344Calcium 1; catalyticBy similarity1
Active sitei357PROSITE-ProRule annotation1
Metal bindingi391Calcium 1; catalyticBy similarity1
Binding sitei440SubstrateBy similarity1
Binding sitei442SubstrateBy similarity1
Binding sitei532SubstrateBy similarity1
Binding sitei559SubstrateBy similarity1
Metal bindingi660Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi662Calcium 2By similarity1
Metal bindingi686Calcium 2By similarity1
Metal bindingi715Calcium 3By similarity1
Metal bindingi716Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi717Calcium 3By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi154 – 1651PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi190 – 2012PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-SSC-1855204. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-delta-4
Phospholipase C-delta-4
Short name:
PLC-delta-4
Gene namesi
Name:PLCD4
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 15

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003068271 – 7721-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4Add BLAST772

Proteomic databases

PaxDbiQ8SPR7.
PeptideAtlasiQ8SPR7.
PRIDEiQ8SPR7.

Expressioni

Gene expression databases

GenevisibleiQ8SPR7. SS.

Interactioni

Subunit structurei

Interacts with GRIP1.By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000017154.

Structurei

3D structure databases

ProteinModelPortaliQ8SPR7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 131PHPROSITE-ProRule annotationAdd BLAST109
Domaini141 – 176EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini177 – 212EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini210 – 244EF-hand 3PROSITE-ProRule annotationAdd BLAST35
Domaini297 – 442PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST146
Domaini503 – 619PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini624 – 729C2PROSITE-ProRule annotationAdd BLAST106

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 60Substrate bindingBy similarityAdd BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi741 – 744PDZ-binding4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi448 – 483Glu-richAdd BLAST36
Compositional biasi489 – 496Poly-Lys8

Domaini

The PDZ-binding motif mediates the interaction with GRIP1.By similarity
The C2 domain mediates pre-localization to the membrane prior to Ca2+ import and non-selective Ca2+-mediated targeting to various cellular membranes.By similarity
The PH domain is not a critical determinant of the membrane localization.By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 3 EF-hand domains.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0169. Eukaryota.
ENOG410XPSW. LUCA.
GeneTreeiENSGT00760000118936.
HOGENOMiHOG000006871.
HOVERGENiHBG053610.
InParanoidiQ8SPR7.
KOiK05857.
OMAiKASEKYA.
OrthoDBiEOG091G02KH.
TreeFamiTF313216.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001192. PI-PLC_fam.
IPR028387. PLC-delta4.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLC_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF31. PTHR10336:SF31. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00169. PH. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00054. EFh. 3 hits.
SM00233. PH. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8SPR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLLQGLSA SPGSGLPINQ DLLLMQKGML MRKVRSKSWK KLRYYRLQDD
60 70 80 90 100
GMTVWHARQA GGRAKPSFSI SDVETVRGGH ESELLRNLAE EFPLEQGFTI
110 120 130 140 150
VFHGRRSNLD LVANSVEEAQ VWMQGLQLLV DFVTNMDQQE RLDQWLSDWF
160 170 180 190 200
QRGDKNQDGR MSFGEVQRLL HLMNVEMDQE HAFQLFQTAD TSQSGTLEGE
210 220 230 240 250
EFVEFYKSLT KRAEVQELFE NFSSDGQKLT LLEFVDFLQE EQKEGERASD
260 270 280 290 300
LALELIDRHE PSDSGKLRHV LSLDGFLSYL CSKDGDIFNP TCLPIYQDMT
310 320 330 340 350
QPLNHYFINS SHNTYLVGDQ LCGQSSVEGY IRALKRGCRC VEVDIWDGPN
360 370 380 390 400
GEPVVYHGHT LTSRIPFKDV VAAIAQYAFQ TSDYPVILSL ENHCSWEQQE
410 420 430 440 450
MIAHHLTEIL GEQLLSTTLD GQLPTQLPSP EELRRKILVK GKKLRTLEED
460 470 480 490 500
LEEEEEEPEE SELEGEQEAE LELEAQFESE PQELSPRSKD KKKKVKAILC
510 520 530 540 550
PALSALVVYL KAVSFYSFAH SREHYRFYEI SSFSEAKAKS LIKESGNEFV
560 570 580 590 600
QHNTWQLSRV YPGGLRTDSS NYNPQEFWNA GCQMVAMNMQ TAGLEMDLCD
610 620 630 640 650
GLFRQNAGCG YVLKPDFLRD AQSSFHPERP ISPSKAQTLL IQVISGQQLP
660 670 680 690 700
KVDDSKEGSI VDPLVRVEIF GVRPDTARQE TSYVENNGFN PYWGQTLCFR
710 720 730 740 750
VLVPELALLR FVVKDYDWKS RNDFVGQYTL PWNCMQQGYR HIHLLSKDGI
760 770
SLHPASIFVH ICIREGIEGV ES
Length:772
Mass (Da):88,075
Last modified:June 1, 2002 - v1
Checksum:i86E5706A74247548
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF498759 mRNA. Translation: AAM18122.1.
RefSeqiNP_999217.1. NM_214052.1.
UniGeneiSsc.191.

Genome annotation databases

EnsembliENSSSCT00000017632; ENSSSCP00000017154; ENSSSCG00000016192.
GeneIDi397119.
KEGGissc:397119.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF498759 mRNA. Translation: AAM18122.1.
RefSeqiNP_999217.1. NM_214052.1.
UniGeneiSsc.191.

3D structure databases

ProteinModelPortaliQ8SPR7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000017154.

Proteomic databases

PaxDbiQ8SPR7.
PeptideAtlasiQ8SPR7.
PRIDEiQ8SPR7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000017632; ENSSSCP00000017154; ENSSSCG00000016192.
GeneIDi397119.
KEGGissc:397119.

Organism-specific databases

CTDi84812.

Phylogenomic databases

eggNOGiKOG0169. Eukaryota.
ENOG410XPSW. LUCA.
GeneTreeiENSGT00760000118936.
HOGENOMiHOG000006871.
HOVERGENiHBG053610.
InParanoidiQ8SPR7.
KOiK05857.
OMAiKASEKYA.
OrthoDBiEOG091G02KH.
TreeFamiTF313216.

Enzyme and pathway databases

ReactomeiR-SSC-1855204. Synthesis of IP3 and IP4 in the cytosol.

Gene expression databases

GenevisibleiQ8SPR7. SS.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001192. PI-PLC_fam.
IPR028387. PLC-delta4.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLC_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF31. PTHR10336:SF31. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00169. PH. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00054. EFh. 3 hits.
SM00233. PH. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLCD4_PIG
AccessioniPrimary (citable) accession number: Q8SPR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.