Reviewed, UniProtKB/Swiss-Prot Q8SPQ7 (FMO3_MACMU)

Names and origin

Protein names

Recommended name:
    Dimethylaniline monooxygenase [N-oxide-forming] 3
    EC=1.14.13.8
Alternative name(s):
    Hepatic flavin-containing monooxygenase 3
      Short name=FMO 3
    Dimethylaniline oxidase 3

Gene names

Name:

FMO3

Organism

Macaca mulatta (Rhesus macaque)

Taxonomic identifier

9544 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Acts on TMA to produce TMA-N-oxide.
Catalytic activity	N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
Cofactor	FAD By similarity.
Subcellular location	Microsome membrane. Endoplasmic reticulum membrane By similarity.
Tissue specificity	Liver.
Sequence similarities	Belongs to the FMO family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro flavin-containing monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 532	531	Dimethylaniline monooxygenase [N-oxide-forming] 3		PRO_0000147655
Regions
	Nucleotide binding	9 – 14	6	FAD Potential
	Nucleotide binding	191 – 196	6	NADP Potential
Amino acid modifications
	Modified residue	401	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8SPQ7-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 73A4D13EE2A24A72	FASTA	532	60,152
	10 20 30 40 50 60 MGKKVAIIGA GVSGLASIRS CLEEGLEPTC FEKSNDIGGL WKFSDHAEEG RASIYKSVFT 70 80 90 100 110 120 NSSKEMMCFP DFPYPDDFPN FMHNSKIQEY LTAFAKEKSL LKYIQFKTFV SSVNKRPDFA 130 140 150 160 170 180 TTGQWDVTTE RDGKRESAVF DAVMVCSGHH VYPNLPKESF PGLNHFKGKC FHSRDYKEPG 190 200 210 220 230 240 VFKGKRVLVV GLGNSGCDIA TELSHTAEQV VISSRSGSWV MSRVWDNGYP WDMVLITRFG 250 260 270 280 290 300 TFLKNNLPTA ISDWLYMKQM NARFKHENYG LMPLNGVLRK EPVFNDELPA CILCGIVSVK 310 320 330 340 350 360 PNVKKFTETS AIFEDGTTFE GIDCVIFATG YSYTYTFLDE SIIKNRNNEI ILFKGVFPPL 370 380 390 400 410 420 LEKSTIAVIG FVQSLGAAIP TVDLQSRWAA RVIKGTCTLP SMEDMMNDIN EKMERKHKWY 430 440 450 460 470 480 GKSETLQTDY IVYMDELSSF IGVKPNIPWL FLTDPKLAME VYFGPCSPYQ FRLVGPGKWP 490 500 510 520 530 GARNAILTQW DRSLKPLQTR VVGRLQKPCF FFHWLKPFAI PILLIAVFLG LT

References

[1]	"Population distribution of human flavin-containing monooxygenase form 3: gene polymorphisms." Cashman J.R., Zhang J., Leushner J., Braun A. Drug Metab. Dispos. 29:1629-1637(2001) [PubMed: 11717182] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY063498 mRNA. Translation: AAL86612.1.
RefSeq	NP_001028065.1.
UniGene	Mmu.3639
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMMUG00000007952. Macaca mulatta. [Contig view]
GeneID	574244.
KEGG	mcc:574244.
Phylogenomic databases
HOVERGEN	Q8SPQ7.
Enzyme and pathway databases
BRENDA	1.14.13.8. 1774.
Family and domain databases
InterPro	IPR012143. dManiline_mOase. IPR000960. Flavin_mOase. IPR002255. Flavin_mOase_3. [Graphical view]
Pfam	PF00743. FMO-like. 1 hit. [Graphical view]
PIRSF	PIRSF000332. FMO. 1 hit.
PRINTS	PR00370. FMOXYGENASE. PR01123. FMOXYGENASE3.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

FMO3_MACMU

Accession

Primary (citable) accession number: Q8SPQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 49 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)