Reviewed, UniProtKB/Swiss-Prot Q8SPQ0 (CH3L1_CAPHI)

Names and origin

Protein names

Recommended name:
    Chitinase-3-like protein 1
Alternative name(s):
    Mammary gland protein 40
      Short name=MGP-40
    BP40

Gene names

Name:

CHI3L1

Organism

Capra hircus (Goat)

Taxonomic identifier

9925 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Capra

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment.
Subunit structure	Monomer.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Lectin
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Chain

22 – 383

362

Chitinase-3-like protein 1

PRO_0000011964

Regions

Region

70 – 71

2

Chitooligosaccharide binding

Region

97 – 100

4

Chitooligosaccharide binding

Region

204 – 207

4

Chitooligosaccharide

Sites

Binding site

141

1

Chitooligosaccharide

Binding site

263

1

Chitooligosaccharide

Binding site

352

1

Chitooligosaccharide

Amino acid modifications

Glycosylation

60

1

N-linked (GlcNAc...)

Glycosylation

367

1

N-linked (GlcNAc...) Potential

Disulfide bond

26 ↔ 51

Disulfide bond

300 ↔ 364

Secondary structure

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383

Helix Strand Turn

Details...

Beta strand

23 – 29

7

Helix

30 – 34

5

Helix

37 – 39

3

Helix

43 – 45

3

Turn

48 – 50

3

Beta strand

52 – 62

11

Beta strand

65 – 67

3

Helix

73 – 86

14

Beta strand

91 – 97

7

Helix

103 – 111

9

Helix

113 – 130

18

Beta strand

133 – 138

6

Turn

144 – 146

3

Helix

147 – 164

18

Helix

165 – 167

3

Beta strand

173 – 178

6

Helix

182 – 188

7

Helix

191 – 195

5

Beta strand

199 – 203

5

Beta strand

213 – 215

3

Helix

237 – 246

10

Helix

251 – 253

3

Beta strand

254 – 270

17

Beta strand

277 – 281

5

Turn

286 – 288

3

Beta strand

293 – 295

3

Helix

296 – 302

7

Turn

303 – 305

3

Beta strand

307 – 311

5

Turn

312 – 315

4

Beta strand

316 – 321

6

Beta strand

324 – 327

4

Helix

331 – 343

13

Beta strand

347 – 352

6

Helix

354 – 356

3

Beta strand

359 – 361

3

Beta strand

363 – 366

4

Helix

371 – 380

10

Sequences

	Sequence		Length	Mass (Da)
	Q8SPQ0-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 17655ED4BE4E9F5F	FASTA	383	42,894
	10 20 30 40 50 60 MGLRASGTGF VVLVLLQSCA AYKLICYYTS WSQYREGDGS CFPDAIDPFL CTHIIYSFAN 70 80 90 100 110 120 ISNNEIDTWE WNDVTLYDTL NTLKNRNPKL KTLLSVGGWN FGPERFSKIA SKTQSRRTFI 130 140 150 160 170 180 KSVPPFLRTH GFDGLDLAWL YPGRRDKRHL TGLVKEMKAE FAREAQAGTE RLLLSAAVSA 190 200 210 220 230 240 GKIAIDRGYD IAQISRHLDF ISLLTYDFHG AWRQTVGHHS PLFRGQEDAS SDRFSNADYA 250 260 270 280 290 300 VSYMLRLGAP ANKLVMGIPT FGRSFTLASS KTDVGAPISG PGIPGRFTKE KGILAYYEIC 310 320 330 340 350 360 DFLHGATTHR FRDQQVPYAT KGNQWVAYDD QESVKNKARY LKNRQLAGAM VWALDLDDFR 370 380 GTFCGQNLTF PLTSAVKDVL AEV

References

[1]

"Crystal structure of a novel regulatory 40-kDa mammary gland protein (MGP-40) secreted during involution." Mohanty A.K., Singh G., Paramasivam M., Saravanan K., Jabeen T., Sharma S., Yadav S., Kaur P., Kumar P., Srinivasan A., Singh T.P. J. Biol. Chem. 278:14451-14460(2003) [PubMed: 12529329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF N-TERMINUS, X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 22-383 IN COMPLEX WITH CHITIN OLIGOMERS. Tissue: Mammary gland.

[2]

"Carbohydrate-binding properties of goat secretory glycoprotein (SPG-40) and its functional implications: structures of the native glycoprotein and its four complexes with chitin-like oligosaccharides." Kumar J., Ethayathulla A.S., Srivastava D.B., Singh N., Sharma S., Kaur P., Srinivasan A., Singh T.P. Acta Crystallogr. D 63:437-446(2007) [PubMed: 17372347] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.09 ANGSTROMS) OF 22-381 IN COMPLEXES WITH OLIGOSACCHARIDES, GLYCOSYLATION AT ASN-60, DISULFIDE BONDS.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AY081150 mRNA. Translation: AAL87007.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LJY	X-ray	2.90	A	22-383	[»]
1SYT	X-ray	2.60	A	22-381	[»]
1ZBV	X-ray	3.21	A	22-381	[»]
1ZBW	X-ray	2.80	A	22-381	[»]
1ZU8	X-ray	3.05	A	22-381	[»]
2AOS	X-ray	2.90	A	22-381	[»]
2B31	X-ray	3.10	A	22-381	[»]
2DSZ	X-ray	2.35	A	22-381	[»]
2DT0	X-ray	2.45	A	22-381	[»]
2DT1	X-ray	2.09	A	22-381	[»]
2DT2	X-ray	2.90	A	22-381	[»]
2DT3	X-ray	2.28	A	22-381	[»]
2O92	X-ray	3.00	A	22-381	[»]
2OLH	X-ray	2.78	A	22-381	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH18. Glycoside Hydrolase Family 18.

Phylogenomic databases

HOVERGEN

Q8SPQ0.

Family and domain databases

InterPro

IPR011583. Chitinase_II.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.

Pfam

PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]

ProDom

PD000471. Chitinase_II. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00636. Glyco_18. 1 hit.
[Graphical view]

PROSITE

PS01095. CHITINASE_18. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CH3L1_CAPHI

Accession

Primary (citable) accession number: Q8SPQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)