Q8SPN5 (RNAS1_MACMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease pancreatic
EC=3.1.27.5

Alternative name(s):
RNase 1
RNase A

Gene names

Name:	RNASE1
Synonyms:	RNS1

Organism

Macaca mulatta (Rhesus macaque)

Taxonomic identifier

9544 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	By similarity
	Chain	25 – 152	128	Ribonuclease pancreatic		PRO_0000030926
Regions
	Region	65 – 69	5	Substrate binding By similarity
Sites
	Active site	36	1	Proton acceptor By similarity
	Active site	143	1	Proton donor By similarity
	Binding site	31	1	Substrate By similarity
	Binding site	34	1	Substrate By similarity
	Binding site	90	1	Substrate By similarity
	Binding site	109	1	Substrate By similarity
Amino acid modifications
	Glycosylation	46	1	N-linked (GlcNAc...) Potential
	Glycosylation	112	1	N-linked (GlcNAc...) Potential
	Disulfide bond	50 ↔ 108		By similarity
	Disulfide bond	64 ↔ 119		By similarity
	Disulfide bond	82 ↔ 134		By similarity
	Disulfide bond	89 ↔ 96		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8SPN5 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 0B7A5B6F35404FE4	FASTA	152	17,030
	10 20 30 40 50 60 MALDKSVILL PLLVLVLLVL GCLGRESRAK KFQRQHMDSG SSPSSNSTYC NQMMKRRSMT 70 80 90 100 110 120 HGRCKPVNTF VHEPLVDVQN VCFQEKVTCK NGQTNCFKSK SSMHITDCRL TNGSRYPNCA 130 140 150 YRTSPKERHI IVACEGSPHV PVHFDASVED ST

References

[1]	"Adaptive evolution of a duplicated pancreatic ribonuclease gene in a leaf-eating monkey." Zhang J., Zhang Y.-P., Rosenberg H.F. Nat. Genet. 30:411-415(2002) [PubMed: 11925567] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF449632 Genomic DNA. Translation: AAL87053.1.
RefSeq	NP_001038203.1. NM_001044738.1.
UniGene	Mmu.1417.
3D structure databases
ProteinModelPortal	Q8SPN5.
SMR	Q8SPN5. Positions 25-151.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8SPN5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMMUT00000024835; ENSMMUP00000023244; ENSMMUG00000017666. ENSMMUT00000024836; ENSMMUP00000023245; ENSMMUG00000017666. ENSMMUT00000040089; ENSMMUP00000033161; ENSMMUG00000017666.
GeneID	704676.
KEGG	mcc:704676.
Organism-specific databases
CTD	6035.
Phylogenomic databases
eggNOG	prNOG19911.
GeneTree	ENSGT00390000007840.
HOVERGEN	HBG008396.
InParanoid	Q8SPN5.
OMA	NCAYRTS.
OrthoDB	EOG4TMR3F.
PhylomeDB	Q8SPN5.
Family and domain databases
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
KO	K01168.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS1_MACMU

Accession

Primary (citable) accession number: Q8SPN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program