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Reviewed, UniProtKB/Swiss-Prot Q8SPN4 (RNAS1_PYGNE)

Last modified June 16, 2009. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Ribonuclease pancreatic
    EC=3.1.27.5
Alternative name(s):
    RNase 1
    RNase A
Gene names
Name: RNASE1
Synonyms: RNS1
OrganismPygathrix nemaeus (Dove langur) (Douc langur)
Taxonomic identifier54133 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Colobinae › Pygathrix

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Protein attributes

Sequence length156 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.

Catalytic activity

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Subunit structure

Monomer By similarity.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the pancreatic ribonuclease family.

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Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionEndonuclease
Hydrolase
Nuclease
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

pancreatic ribonuclease activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 By similarity
Chain29 – 156128Ribonuclease pancreatic
PRO_0000030938

Regions

Region69 – 735Substrate binding By similarity

Sites

Active site401Proton acceptor By similarity
Active site1471Proton donor By similarity
Binding site351Substrate By similarity
Binding site381Substrate By similarity
Binding site941Substrate By similarity
Binding site1131Substrate By similarity

Amino acid modifications

Glycosylation621N-linked (GlcNAc...) Potential
Glycosylation1161N-linked (GlcNAc...) Potential
Disulfide bond54 ↔ 112 By similarity
Disulfide bond68 ↔ 123 By similarity
Disulfide bond86 ↔ 138 By similarity
Disulfide bond93 ↔ 100 By similarity

Experimental info

Mutagenesis291R → G: Reduces activity towards double stranded RNA. Ref.1
Mutagenesis321R → Q: Slightly reduces activity towards double stranded RNA. Ref.1
Mutagenesis341K → E: Reduces activity towards double stranded RNA. Ref.1
Mutagenesis601R → L: Strongly reduces activity towards double stranded RNA. Ref.1
Mutagenesis671R → W: Reduces activity towards double stranded RNA. Ref.1
Mutagenesis701P → S: Reduces activity towards double stranded RNA. Ref.1
Mutagenesis1111D → E: Strongly reduces activity towards double stranded RNA. Ref.1
Mutagenesis1261R → Q: Reduces activity towards double stranded RNA. Ref.1
Mutagenesis1501A → D: Reduces activity towards double stranded RNA. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q8SPN4-1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 8168684878BE9436

FASTA15617,498
        10         20         30         40         50         60 
MALDKSVILL PLLVVVLLVL GWAQPSLGRE SRAKKFQRQH MDSGSSPSSS STYCNQMMKR 

        70         80         90        100        110        120 
RNMTQGRCKP VNTFVHEPLV DVQNVCFQEK VTCKNGQTNC FKSNSKMHIT DCRLTNGSKY 

       130        140        150 
PNCAYRTSPK ERHIIVACEG SPYVPVHFDA SVEDST

« Hide

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References

[1]"Adaptive evolution of a duplicated pancreatic ribonuclease gene in a leaf-eating monkey."
Zhang J., Zhang Y.-P., Rosenberg H.F.
Nat. Genet. 30:411-415(2002) [PubMed: 11925567] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF ARG-29; ARG-32; LYS-34; ARG-60; ARG-67; PRO-70; ASP-111; ARG-126 AND ALA-150.

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Cross-references

Sequence databases

AF449642 Genomic DNA. Translation: AAL87063.1.

3D structure databases

HSSPHSSP built from PDB template 1SRN based on UniProtKB P00656.
SMRQ8SPN4. Positions 30-155.
ModBaseSearch...

Phylogenomic databases

HOVERGENQ8SPN4.

Enzyme and pathway databases

BRENDA3.1.27.5. 306279.

Family and domain databases

InterProIPR001427. RNaseA.
[Graphical view]
Gene3DG3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHERPTHR11437. RNaseA. 1 hit.
PfamPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSPR00794. RIBONUCLEASE.
ProDomPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00092. RNAse_Pc. 1 hit.
[Graphical view]
PROSITEPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameRNAS1_PYGNE
AccessionPrimary (citable) accession number: Q8SPN4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 1, 2002
Last modified: June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents