Reviewed, UniProtKB/Swiss-Prot Q8SPK1 (CP4AO_PIG)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 4A24
Alternative name(s):
    CYPIVA24
    Fatty acid omega-hydroxylase
    EC=1.14.15.3

Gene names

Name:

CYP4A24

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the omega- and (omega-1)-hydroxylation of various fatty acids such as laurate and palmitate. Has no activity toward taurochenodeoxycholic acid.
Catalytic activity	Octane + reduced rubredoxin + O2 = 1-octanol + oxidized rubredoxin + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alkane 1-monooxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Cytochrome P450 4A24		PRO_0000280743
Regions
	Transmembrane	6 – 26	21	Potential
	Transmembrane	112 – 132	21	Potential
Sites
	Metal binding	451	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	202	1	A → V in CAE52533. Ref.2
	Sequence conflict	224	1	I → T in CAE52533. Ref.2
	Sequence conflict	246	1	N → S in CAE52533. Ref.2
	Sequence conflict	293	1	R → Q in CAE52533. Ref.2
	Sequence conflict	382 – 384	3	GVS → SVG in CAE52533. Ref.2
	Sequence conflict	404	1	T → I in CAE52533. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8SPK1-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: DB7F815966DA7D7B	FASTA	504	57,199
	10 20 30 40 50 60 MTVPALASAS GLLQVASLLG LLLLLLKAAQ LYLHRQWLLK ALQQFPSPPS HWLYGHSREF 70 80 90 100 110 120 QEESELPPLL KRVEKYPSAC ALWRWGTRAM VLVYDPDYMK VVLARSDPKN SVVYRLLIPW 130 140 150 160 170 180 IGCGLLLLNG QTWFQRRRML TPAFHYDILK PYVGLMAKSV QVMLDKWEQL VAQDPRLEIV 190 200 210 220 230 240 GPVSLMTLDT IMKCAFSHQG SAQTDGDSHS YIQAIWDLKN LFSIRTKSAF LQNDIIYRLS 250 260 270 280 290 300 PEGRKNHRAA RIAHQHTDRV IQLRKAQLQK QGEMENVRKK RHLDFLDILL LARMEKGNSL 310 320 330 340 350 360 SDTDLRAEVD TFMFEGHDTT ASGISWILYA LASHPEHQQR CREEIQGLLG DGTSITWDHL 370 380 390 400 410 420 DQMPYTTMCI KEALRLYPPV PGVSRELSKP ITFPDGRSLP AGITLSLSIY GLHHNPQVWP 430 440 450 460 470 480 NPEEFDPSRF APGSARHSHA FMPFSGGSRN CIGKQFAMNE MKVAVALTLL RFELAPDPSR 490 500 KPIATPEVVL NSKNGIHLKL RKLP

References

[1]	"Cloning and expression of two novel pig liver and kidney fatty acid hydroxylases [cytochrome P450 (CYP)4A24 and CYP4A25]." Lundell K. Biochem. J. 363:297-303(2002) [PubMed: 11931657] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The porcine taurochenodeoxycholic acid 6alpha-hydroxylase (CYP4A21) gene: evolution by gene duplication and gene conversion." Lundell K. Biochem. J. 378:1053-1058(2004) [PubMed: 14641109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42 AND 83-448.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ318096 mRNA. Translation: CAC85662.1. AJ586860 Genomic DNA. Translation: CAE52547.1. AJ586619 Genomic DNA. Translation: CAE52533.1.
UniGene	Ssc.49966
3D structure databases
HSSP	HSSP built from PDB template 1TQN based on UniProtKB P08684.
SMR	Q8SPK1. Positions 45-503.
ModBase	Search...
Genome annotation databases
Ensembl	ENSSSCT00000004307; ENSSSCP00000004207; ENSSSCG00000003893; Sus scrofa. [Genome view]
Phylogenomic databases
HOVERGEN	Q8SPK1.
Enzyme and pathway databases
BRENDA	1.14.15.3. 249.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP4AO_PIG

Accession

Primary (citable) accession number: Q8SPK1
Secondary accession number(s): Q70BW1, Q70BZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	June 1, 2002
Last modified:	January 19, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)