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Protein

Junction plakoglobin

Gene

JUP

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity).By similarity

GO - Molecular functioni

  1. alpha-catenin binding Source: GO_Central
  2. cadherin binding Source: GO_Central
  3. protein homodimerization activity Source: BHF-UCL
  4. protein phosphatase binding Source: GO_Central
  5. signal transducer activity Source: InterPro
  6. transcription coactivator activity Source: Ensembl

GO - Biological processi

  1. adherens junction assembly Source: InterPro
  2. bundle of His cell to Purkinje myocyte communication Source: Ensembl
  3. cell migration Source: Ensembl
  4. cellular response to indole-3-methanol Source: Ensembl
  5. desmosome assembly Source: Ensembl
  6. detection of mechanical stimulus Source: Ensembl
  7. endothelial cell-cell adhesion Source: BHF-UCL
  8. establishment of protein localization to plasma membrane Source: Ensembl
  9. positive regulation of protein import into nucleus Source: Ensembl
  10. positive regulation of sequence-specific DNA binding transcription factor activity Source: Ensembl
  11. regulation of cell fate specification Source: GO_Central
  12. regulation of cell proliferation Source: Ensembl
  13. regulation of heart rate by cardiac conduction Source: Ensembl
  14. skin development Source: Ensembl
  15. ventricular cardiac muscle cell action potential Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiREACT_321030. Adherens junctions interactions.
REACT_324959. VEGFR2 mediated vascular permeability.

Names & Taxonomyi

Protein namesi
Recommended name:
Junction plakoglobin
Alternative name(s):
Desmoplakin III
Desmoplakin-3
Gene namesi
Name:JUPImported
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 19

Subcellular locationi

  1. Cell junctionadherens junction By similarity
  2. Cell junctiondesmosome By similarity
  3. Cytoplasmcytoskeleton By similarity
  4. Membrane By similarity; Peripheral membrane protein By similarity

  5. Note: Cytoplasmic in a soluble and membrane-associated form.By similarity

GO - Cellular componenti

  1. catenin complex Source: GO_Central
  2. cell-cell adherens junction Source: AgBase
  3. cytoplasmic side of plasma membrane Source: BHF-UCL
  4. cytoskeleton Source: BHF-UCL
  5. cytosol Source: BHF-UCL
  6. desmosome Source: BHF-UCL
  7. extracellular vesicular exosome Source: Ensembl
  8. focal adhesion Source: Ensembl
  9. gamma-catenin-TCF7L2 complex Source: Ensembl
  10. intercalated disc Source: Ensembl
  11. intermediate filament Source: Ensembl
  12. nucleus Source: GO_Central
  13. protein-DNA complex Source: Ensembl
  14. Z disc Source: Ensembl
  15. zonula adherens Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 745745Junction plakoglobinPRO_0000064277Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Glycosylationi14 – 141O-linked (GlcNAc)By similarity
Modified residuei99 – 991PhosphoserineBy similarity
Modified residuei125 – 1251PhosphoserineBy similarity
Modified residuei182 – 1821PhosphoserineBy similarity
Modified residuei665 – 6651PhosphoserineBy similarity
Modified residuei730 – 7301PhosphoserineBy similarity

Post-translational modificationi

May be phosphorylated by FER.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8SPJ1.
PRIDEiQ8SPJ1.

Interactioni

Subunit structurei

Homodimer. Component of an E-cadherin/catenin adhesion complex composed of at least E-cadherin/CDH1 and gamma-catenin/JUP, and possibly alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Interacts with MUC1. Interacts with CAV1. Interacts with PTPRJ. Interacts with DSG1. Interacts with DSC1 and DSC2. Interacts with PKP2 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8SPJ1.
SMRiQ8SPJ1. Positions 118-672.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati132 – 17140ARM 1Sequence AnalysisAdd
BLAST
Repeati172 – 21544ARM 2Add
BLAST
Repeati216 – 25540ARM 3Add
BLAST
Repeati258 – 29740ARM 4Add
BLAST
Repeati298 – 34144ARM 5Add
BLAST
Repeati342 – 38140ARM 6Add
BLAST
Repeati383 – 42038ARM 7Add
BLAST
Repeati423 – 46442ARM 8Add
BLAST
Repeati470 – 51041ARM 9Add
BLAST
Repeati512 – 55140ARM 10Add
BLAST
Repeati574 – 61340ARM 11Add
BLAST
Repeati615 – 66147ARM 12Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni132 – 297166Interaction with DSC1 and DSG1By similarityAdd
BLAST
Regioni574 – 66188Interaction with DSC1By similarityAdd
BLAST

Domaini

The entire ARM repeats region mediates binding to CDH1/E-cadherin. The N-terminus and first three ARM repeats are sufficient for binding to DSG1. The N-terminus and first ARM repeat are sufficient for association with CTNNA1. DSC1 association requires both ends of the ARM repeat region (By similarity).By similarity

Sequence similaritiesi

Belongs to the beta-catenin family.Curated
Contains 12 ARM repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG297695.
GeneTreeiENSGT00730000110821.
HOGENOMiHOG000230958.
HOVERGENiHBG000919.
InParanoidiQ8SPJ1.
KOiK10056.
OMAiMNLIEQP.
OrthoDBiEOG7X9G6B.
TreeFamiTF317997.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR013284. Beta-catenin.
IPR030461. Plakoglobin.
[Graphical view]
PANTHERiPTHR23315:SF12. PTHR23315:SF12. 1 hit.
PfamiPF00514. Arm. 3 hits.
[Graphical view]
PRINTSiPR01869. BCATNINFAMLY.
SMARTiSM00185. ARM. 12 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 9 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8SPJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVMNLIEQP IKVTEWQQTY TYDSGIHSGA NTCVPSLSSK GLIEEDEACG
60 70 80 90 100
RQYTLKKTTT YTQSVPPGQG DLEYQMSTTA RAKRVREAMC PGVTGEDSSL
110 120 130 140 150
LLTTQVEGQT TNLQRLAEPS QLLKSAIVHL INYQDDAELA TRALPELTKL
160 170 180 190 200
LNDEDPVVVT KAAMIVNQLS KKEASRRALM GSPQLVAAVV RTMQNTSDLD
210 220 230 240 250
TARCTTSILH NLSHHREGLL AIFKSGGIPA LVRMLSSPVE SVLFYAITTL
260 270 280 290 300
HNLLLYQEGA KMAVRLADGL QKMVPLLNKN NPKFLAITTD CLQLLAYGNQ
310 320 330 340 350
ESKLIILANG GPQALVQIMR NYSYEKLLWT TSRVLKVLSV CPSNKPAIVE
360 370 380 390 400
AGGMQALGKH LTSNSPRLVQ NCLWTLRNLS DVATKQEGLE SVLKILVNQL
410 420 430 440 450
SVDDVNVLTC ATGTLSNLTC NNSKNKTLVT QNSGVEALIH AILRAGDKDD
460 470 480 490 500
ITEPAVCALR HLTSRHPEAE MAQNSVRLNY GIPAIVKLLN QPNQWPLVKA
510 520 530 540 550
TIGLIRNLAL CPANHAPLQE AAVIPRLVQL LVKAHQDAQR HVAAGTQQPY
560 570 580 590 600
TDGVRMEEIV EGCTGALHIL ARDPMNRMEI FRLNTIPLFV QLLYSSVENI
610 620 630 640 650
QRVAAGVLCE LAQDKEAADA IDAEGASAPL MELLHSRNEG TATYAAAVLF
660 670 680 690 700
RISEDKNPDY RKRVSVELTN SLFKHDPAAW EAAQSMIPMN EPYADDMDAT
710 720 730 740
YRPMYSSDVP MDPLEMHMDM DGDYPIDTYS DGLRPPYATA DHMLA
Length:745
Mass (Da):81,821
Last modified:June 1, 2002 - v1
Checksum:iCCF44BAC1FEA469C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti92 – 921G → S in AAI18115 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF497524
, AF497518, AF497519, AF497520, AF497521, AF497522, AF497523 Genomic DNA. Translation: AAM19329.1.
BC118114 mRNA. Translation: AAI18115.1.
PIRiB32905.
RefSeqiNP_001004024.1. NM_001004024.2.
XP_005220741.1. XM_005220684.1.
XP_010814523.1. XM_010816221.1.
XP_010814524.1. XM_010816222.1.
UniGeneiBt.20230.

Genome annotation databases

EnsembliENSBTAT00000023522; ENSBTAP00000023522; ENSBTAG00000017685.
GeneIDi445543.
KEGGibta:445543.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF497524
, AF497518, AF497519, AF497520, AF497521, AF497522, AF497523 Genomic DNA. Translation: AAM19329.1.
BC118114 mRNA. Translation: AAI18115.1.
PIRiB32905.
RefSeqiNP_001004024.1. NM_001004024.2.
XP_005220741.1. XM_005220684.1.
XP_010814523.1. XM_010816221.1.
XP_010814524.1. XM_010816222.1.
UniGeneiBt.20230.

3D structure databases

ProteinModelPortaliQ8SPJ1.
SMRiQ8SPJ1. Positions 118-672.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ8SPJ1.
PRIDEiQ8SPJ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000023522; ENSBTAP00000023522; ENSBTAG00000017685.
GeneIDi445543.
KEGGibta:445543.

Organism-specific databases

CTDi3728.

Phylogenomic databases

eggNOGiNOG297695.
GeneTreeiENSGT00730000110821.
HOGENOMiHOG000230958.
HOVERGENiHBG000919.
InParanoidiQ8SPJ1.
KOiK10056.
OMAiMNLIEQP.
OrthoDBiEOG7X9G6B.
TreeFamiTF317997.

Enzyme and pathway databases

ReactomeiREACT_321030. Adherens junctions interactions.
REACT_324959. VEGFR2 mediated vascular permeability.

Miscellaneous databases

NextBioi20832184.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR013284. Beta-catenin.
IPR030461. Plakoglobin.
[Graphical view]
PANTHERiPTHR23315:SF12. PTHR23315:SF12. 1 hit.
PfamiPF00514. Arm. 3 hits.
[Graphical view]
PRINTSiPR01869. BCATNINFAMLY.
SMARTiSM00185. ARM. 12 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 9 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Role of plakoglobin in bovine cardiomyopathy with woolly haircoat syndrome."
    Simpson M.A., Crosby A.H.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiPLAK_BOVIN
AccessioniPrimary (citable) accession number: Q8SPJ1
Secondary accession number(s): Q17QY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: June 1, 2002
Last modified: April 29, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.