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Q8S9J1

- PPOCM_ARATH

UniProt

Q8S9J1 - PPOCM_ARATH

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Protein

Protoporphyrinogen oxidase 2, chloroplastic/mitochondrial

Gene
PPOX2, HEMG2, MEE61, PPO2, At5g14220, F18O22.10, MUA22.22
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX By similarity.

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactori

Binds 1 FAD per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei268 – 2681FAD; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi23 – 286FAD By similarity
Nucleotide bindingi46 – 472FAD By similarity
Nucleotide bindingi68 – 714FAD By similarity
Nucleotide bindingi475 – 4773FAD By similarity

GO - Molecular functioni

  1. oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. chlorophyll biosynthetic process Source: UniProtKB-UniPathway
  2. embryo development ending in seed dormancy Source: TAIR
  3. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciARA:AT5G14220-MONOMER.
ARA:GQT-1544-MONOMER.
UniPathwayiUPA00251; UER00324.
UPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase 2, chloroplastic/mitochondrial (EC:1.3.3.4)
Short name:
PPO2
Alternative name(s):
Protein MATERNAL EFFECT EMBRYO ARREST 61
Gene namesi
Name:PPOX2
Synonyms:HEMG2, MEE61, PPO2
Ordered Locus Names:At5g14220
ORF Names:F18O22.10, MUA22.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G14220.

Subcellular locationi

Plastidchloroplast. Mitochondrion 2 Publications

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast envelope Source: TAIR
  3. mitochondrion Source: TAIR
  4. plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryo development arrested at one-cell zygotic stage.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2222Chloroplast and mitochondrion Reviewed predictionAdd
BLAST
Chaini23 – 508486Protoporphyrinogen oxidase 2, chloroplastic/mitochondrialPRO_0000422669Add
BLAST

Proteomic databases

PRIDEiQ8S9J1.

Expressioni

Gene expression databases

GenevestigatoriQ8S9J1.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G14220.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8S9J1.
SMRiQ8S9J1. Positions 16-499.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1232.
HOGENOMiHOG000269479.
InParanoidiQ8S9J1.
KOiK00231.
OMAiISIYLGF.
PhylomeDBiQ8S9J1.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8S9J1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MASGAVADHQ IEAVSGKRVA VVGAGVSGLA AAYKLKSRGL NVTVFEADGR    50
VGGKLRSVMQ NGLIWDEGAN TMTEAEPEVG SLLDDLGLRE KQQFPISQKK 100
RYIVRNGVPV MLPTNPIELV TSSVLSTQSK FQILLEPFLW KKKSSKVSDA 150
SAEESVSEFF QRHFGQEVVD YLIDPFVGGT SAADPDSLSM KHSFPDLWNV 200
EKSFGSIIVG AIRTKFAAKG GKSRDTKSSP GTKKGSRGSF SFKGGMQILP 250
DTLCKSLSHD EINLDSKVLS LSYNSGSRQE NWSLSCVSHN ETQRQNPHYD 300
AVIMTAPLCN VKEMKVMKGG QPFQLNFLPE INYMPLSVLI TTFTKEKVKR 350
PLEGFGVLIP SKEQKHGFKT LGTLFSSMMF PDRSPSDVHL YTTFIGGSRN 400
QELAKASTDE LKQVVTSDLQ RLLGVEGEPV SVNHYYWRKA FPLYDSSYDS 450
VMEAIDKMEN DLPGFFYAGN HRGGLSVGKS IASGCKAADL VISYLESCSN 500
DKKPNDSL 508
Length:508
Mass (Da):55,630
Last modified:June 1, 2002 - v1
Checksum:iF092957F1F3DD268
GO
Isoform 2 (identifier: Q8S9J1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     112-141: Missing.

Show »
Length:478
Mass (Da):52,218
Checksum:iEEB49AD0E4D8F129
GO

Sequence cautioni

The sequence BAB08301.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAB87761.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei112 – 14130Missing in isoform 2. VSP_046550Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB007650 Genomic DNA. Translation: BAB08301.1. Sequence problems.
AL163817 Genomic DNA. Translation: CAB87761.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92001.1.
CP002688 Genomic DNA. Translation: AED92002.1.
AY075665 mRNA. Translation: AAL77672.1.
AY101523 mRNA. Translation: AAM26644.1.
PIRiT48595.
RefSeqiNP_001190307.1. NM_001203378.1. [Q8S9J1-2]
NP_196926.2. NM_121426.4. [Q8S9J1-1]
UniGeneiAt.20436.

Genome annotation databases

EnsemblPlantsiAT5G14220.1; AT5G14220.1; AT5G14220. [Q8S9J1-1]
GeneIDi831272.
KEGGiath:AT5G14220.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB007650 Genomic DNA. Translation: BAB08301.1 . Sequence problems.
AL163817 Genomic DNA. Translation: CAB87761.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED92001.1 .
CP002688 Genomic DNA. Translation: AED92002.1 .
AY075665 mRNA. Translation: AAL77672.1 .
AY101523 mRNA. Translation: AAM26644.1 .
PIRi T48595.
RefSeqi NP_001190307.1. NM_001203378.1. [Q8S9J1-2 ]
NP_196926.2. NM_121426.4. [Q8S9J1-1 ]
UniGenei At.20436.

3D structure databases

ProteinModelPortali Q8S9J1.
SMRi Q8S9J1. Positions 16-499.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G14220.1-P.

Proteomic databases

PRIDEi Q8S9J1.

Protocols and materials databases

DNASUi 831272.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G14220.1 ; AT5G14220.1 ; AT5G14220 . [Q8S9J1-1 ]
GeneIDi 831272.
KEGGi ath:AT5G14220.

Organism-specific databases

TAIRi AT5G14220.

Phylogenomic databases

eggNOGi COG1232.
HOGENOMi HOG000269479.
InParanoidi Q8S9J1.
KOi K00231.
OMAi ISIYLGF.
PhylomeDBi Q8S9J1.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00324 .
UPA00668 .
BioCyci ARA:AT5G14220-MONOMER.
ARA:GQT-1544-MONOMER.

Gene expression databases

Genevestigatori Q8S9J1.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.660.20. 1 hit.
InterProi IPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00562. proto_IX_ox. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
    Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis."
    Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S., Capron A., Xie L.-F., Ye D., Sundaresan V.
    Development 132:603-614(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  6. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
  7. "Defining the protein complex proteome of plant mitochondria."
    Klodmann J., Senkler M., Rode C., Braun H.-P.
    Plant Physiol. 157:587-598(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
  8. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPPOCM_ARATH
AccessioniPrimary (citable) accession number: Q8S9J1
Secondary accession number(s): F4K5H8, Q9FMS9, Q9LYA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: June 1, 2002
Last modified: September 3, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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