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Protein

Protoporphyrinogen oxidase 2, chloroplastic/mitochondrial

Gene

PPOX2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.By similarity

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.
Proteins known to be involved in this subpathway in this organism are:
  1. Protoporphyrinogen oxidase 2, chloroplastic/mitochondrial (PPOX2), Protoporphyrinogen oxidase (AXX17_At5g13680), Protoporphyrinogen oxidase (AXX17_At4g02130), Protoporphyrinogen oxidase 1, chloroplastic (PPOX1), Protoporphyrinogen oxidase (HEMG2), Protoporphyrinogen oxidase (HEMG2)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: chlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei268FAD; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi23 – 28FADBy similarity6
Nucleotide bindingi46 – 47FADBy similarity2
Nucleotide bindingi68 – 71FADBy similarity4
Nucleotide bindingi475 – 477FADBy similarity3

GO - Molecular functioni

GO - Biological processi

  • chlorophyll biosynthetic process Source: UniProtKB-UniPathway
  • embryo development ending in seed dormancy Source: TAIR
  • protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionOxidoreductase
Biological processChlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis
LigandFAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-ATH-189451 Heme biosynthesis
UniPathwayiUPA00251; UER00324
UPA00668

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase 2, chloroplastic/mitochondrial (EC:1.3.3.4)
Short name:
PPO2
Alternative name(s):
Protein MATERNAL EFFECT EMBRYO ARREST 61
Gene namesi
Name:PPOX2
Synonyms:HEMG2, MEE61, PPO2
Ordered Locus Names:At5g14220
ORF Names:F18O22.10, MUA22.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G14220
TAIRilocus:2145603 AT5G14220

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryo development arrested at one-cell zygotic stage.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 22Chloroplast and mitochondrionSequence analysisAdd BLAST22
ChainiPRO_000042266923 – 508Protoporphyrinogen oxidase 2, chloroplastic/mitochondrialAdd BLAST486

Proteomic databases

PaxDbiQ8S9J1
ProMEXiQ8S9J1

PTM databases

iPTMnetiQ8S9J1

Expressioni

Gene expression databases

ExpressionAtlasiQ8S9J1 baseline and differential
GenevisibleiQ8S9J1 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G14220.1

Structurei

3D structure databases

ProteinModelPortaliQ8S9J1
SMRiQ8S9J1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1276 Eukaryota
COG1232 LUCA
HOGENOMiHOG000269479
InParanoidiQ8S9J1
KOiK00231
OMAiEHNQAVQ
OrthoDBiEOG0936077I
PhylomeDBiQ8S9J1

Family and domain databases

Gene3Di3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR002937 Amino_oxidase
IPR036188 FAD/NAD-bd_sf
IPR004572 Protoporphyrinogen_oxidase
PfamiView protein in Pfam
PF01593 Amino_oxidase, 1 hit
SUPFAMiSSF51905 SSF51905, 2 hits
TIGRFAMsiTIGR00562 proto_IX_ox, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8S9J1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASGAVADHQ IEAVSGKRVA VVGAGVSGLA AAYKLKSRGL NVTVFEADGR
60 70 80 90 100
VGGKLRSVMQ NGLIWDEGAN TMTEAEPEVG SLLDDLGLRE KQQFPISQKK
110 120 130 140 150
RYIVRNGVPV MLPTNPIELV TSSVLSTQSK FQILLEPFLW KKKSSKVSDA
160 170 180 190 200
SAEESVSEFF QRHFGQEVVD YLIDPFVGGT SAADPDSLSM KHSFPDLWNV
210 220 230 240 250
EKSFGSIIVG AIRTKFAAKG GKSRDTKSSP GTKKGSRGSF SFKGGMQILP
260 270 280 290 300
DTLCKSLSHD EINLDSKVLS LSYNSGSRQE NWSLSCVSHN ETQRQNPHYD
310 320 330 340 350
AVIMTAPLCN VKEMKVMKGG QPFQLNFLPE INYMPLSVLI TTFTKEKVKR
360 370 380 390 400
PLEGFGVLIP SKEQKHGFKT LGTLFSSMMF PDRSPSDVHL YTTFIGGSRN
410 420 430 440 450
QELAKASTDE LKQVVTSDLQ RLLGVEGEPV SVNHYYWRKA FPLYDSSYDS
460 470 480 490 500
VMEAIDKMEN DLPGFFYAGN HRGGLSVGKS IASGCKAADL VISYLESCSN

DKKPNDSL
Length:508
Mass (Da):55,630
Last modified:June 1, 2002 - v1
Checksum:iF092957F1F3DD268
GO
Isoform 2 (identifier: Q8S9J1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     112-141: Missing.

Show »
Length:478
Mass (Da):52,218
Checksum:iEEB49AD0E4D8F129
GO

Sequence cautioni

The sequence BAB08301 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB87761 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_046550112 – 141Missing in isoform 2. CuratedAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007650 Genomic DNA Translation: BAB08301.1 Sequence problems.
AL163817 Genomic DNA Translation: CAB87761.1 Sequence problems.
CP002688 Genomic DNA Translation: AED92001.1
CP002688 Genomic DNA Translation: AED92002.1
AY075665 mRNA Translation: AAL77672.1
AY101523 mRNA Translation: AAM26644.1
PIRiT48595
RefSeqiNP_001190307.1, NM_001203378.1 [Q8S9J1-2]
NP_196926.2, NM_121426.5 [Q8S9J1-1]
UniGeneiAt.20436

Genome annotation databases

EnsemblPlantsiAT5G14220.1; AT5G14220.1; AT5G14220 [Q8S9J1-1]
AT5G14220.2; AT5G14220.2; AT5G14220 [Q8S9J1-2]
GeneIDi831272
GrameneiAT5G14220.1; AT5G14220.1; AT5G14220 [Q8S9J1-1]
AT5G14220.2; AT5G14220.2; AT5G14220 [Q8S9J1-2]
KEGGiath:AT5G14220

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPPOCM_ARATH
AccessioniPrimary (citable) accession number: Q8S9J1
Secondary accession number(s): F4K5H8, Q9FMS9, Q9LYA7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: June 1, 2002
Last modified: April 25, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health