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Q8S9J1 (PPOCM_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protoporphyrinogen oxidase 2, chloroplastic/mitochondrial

Short name=PPO2
EC=1.3.3.4
Alternative name(s):
Protein MATERNAL EFFECT EMBRYO ARREST 61
Gene names
Name:PPOX2
Synonyms:HEMG2, MEE61, PPO2
Ordered Locus Names:At5g14220
ORF Names:F18O22.10, MUA22.22
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX By similarity.

Catalytic activity

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactor

Binds 1 FAD per subunit By similarity.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.

Porphyrin-containing compound metabolism; chlorophyll biosynthesis.

Subcellular location

Plastidchloroplast. Mitochondrion Ref.6 Ref.7.

Disruption phenotype

Embryo development arrested at one-cell zygotic stage. Ref.5

Sequence similarities

Belongs to the protoporphyrinogen oxidase family.

Sequence caution

The sequence BAB08301.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAB87761.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8S9J1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8S9J1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     112-141: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2222Chloroplast and mitochondrion Potential
Chain23 – 508486Protoporphyrinogen oxidase 2, chloroplastic/mitochondrial
PRO_0000422669

Regions

Nucleotide binding23 – 286FAD By similarity
Nucleotide binding46 – 472FAD By similarity
Nucleotide binding68 – 714FAD By similarity
Nucleotide binding475 – 4773FAD By similarity

Sites

Binding site2681FAD; via amide nitrogen and carbonyl oxygen By similarity

Natural variations

Alternative sequence112 – 14130Missing in isoform 2.
VSP_046550

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: F092957F1F3DD268

FASTA50855,630
        10         20         30         40         50         60 
MASGAVADHQ IEAVSGKRVA VVGAGVSGLA AAYKLKSRGL NVTVFEADGR VGGKLRSVMQ 

        70         80         90        100        110        120 
NGLIWDEGAN TMTEAEPEVG SLLDDLGLRE KQQFPISQKK RYIVRNGVPV MLPTNPIELV 

       130        140        150        160        170        180 
TSSVLSTQSK FQILLEPFLW KKKSSKVSDA SAEESVSEFF QRHFGQEVVD YLIDPFVGGT 

       190        200        210        220        230        240 
SAADPDSLSM KHSFPDLWNV EKSFGSIIVG AIRTKFAAKG GKSRDTKSSP GTKKGSRGSF 

       250        260        270        280        290        300 
SFKGGMQILP DTLCKSLSHD EINLDSKVLS LSYNSGSRQE NWSLSCVSHN ETQRQNPHYD 

       310        320        330        340        350        360 
AVIMTAPLCN VKEMKVMKGG QPFQLNFLPE INYMPLSVLI TTFTKEKVKR PLEGFGVLIP 

       370        380        390        400        410        420 
SKEQKHGFKT LGTLFSSMMF PDRSPSDVHL YTTFIGGSRN QELAKASTDE LKQVVTSDLQ 

       430        440        450        460        470        480 
RLLGVEGEPV SVNHYYWRKA FPLYDSSYDS VMEAIDKMEN DLPGFFYAGN HRGGLSVGKS 

       490        500 
IASGCKAADL VISYLESCSN DKKPNDSL 

« Hide

Isoform 2 [UniParc].

Checksum: EEB49AD0E4D8F129
Show »

FASTA47852,218

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis."
Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S., Capron A., Xie L.-F., Ye D., Sundaresan V.
Development 132:603-614(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[6]"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[7]"Defining the protein complex proteome of plant mitochondria."
Klodmann J., Senkler M., Rode C., Braun H.-P.
Plant Physiol. 157:587-598(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[8]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB007650 Genomic DNA. Translation: BAB08301.1. Sequence problems.
AL163817 Genomic DNA. Translation: CAB87761.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92001.1.
CP002688 Genomic DNA. Translation: AED92002.1.
AY075665 mRNA. Translation: AAL77672.1.
AY101523 mRNA. Translation: AAM26644.1.
PIRT48595.
RefSeqNP_001190307.1. NM_001203378.1.
NP_196926.2. NM_121426.4.
UniGeneAt.20436.

3D structure databases

ProteinModelPortalQ8S9J1.
SMRQ8S9J1. Positions 16-499.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G14220.1-P.

Proteomic databases

PRIDEQ8S9J1.

Protocols and materials databases

DNASU831272.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G14220.1; AT5G14220.1; AT5G14220. [Q8S9J1-1]
GeneID831272.
KEGGath:AT5G14220.

Organism-specific databases

TAIRAT5G14220.

Phylogenomic databases

eggNOGCOG1232.
HOGENOMHOG000269479.
InParanoidQ8S9J1.
KOK00231.
OMAFFYAGNH.
PhylomeDBQ8S9J1.
ProtClustDBPLN02576.

Enzyme and pathway databases

BioCycARA:AT5G14220-MONOMER.
ARA:GQT-1544-MONOMER.
UniPathwayUPA00251; UER00324.
UPA00668.

Gene expression databases

GenevestigatorQ8S9J1.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.660.20. 1 hit.
InterProIPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
IPR004572. Protoporphyrinogen_oxidase.
IPR027418. Protoporphyrinogen_oxidase_C.
[Graphical view]
PfamPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00562. proto_IX_ox. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPOCM_ARATH
AccessionPrimary (citable) accession number: Q8S9J1
Secondary accession number(s): F4K5H8, Q9FMS9, Q9LYA7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names