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Q8S9J1

- PPOCM_ARATH

UniProt

Q8S9J1 - PPOCM_ARATH

Protein

Protoporphyrinogen oxidase 2, chloroplastic/mitochondrial

Gene

PPOX2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.By similarity

    Catalytic activityi

    Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

    Cofactori

    Binds 1 FAD per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei268 – 2681FAD; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi23 – 286FADBy similarity
    Nucleotide bindingi46 – 472FADBy similarity
    Nucleotide bindingi68 – 714FADBy similarity
    Nucleotide bindingi475 – 4773FADBy similarity

    GO - Molecular functioni

    1. oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. chlorophyll biosynthetic process Source: UniProtKB-UniPathway
    2. embryo development ending in seed dormancy Source: TAIR
    3. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciARA:AT5G14220-MONOMER.
    ARA:GQT-1544-MONOMER.
    UniPathwayiUPA00251; UER00324.
    UPA00668.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protoporphyrinogen oxidase 2, chloroplastic/mitochondrial (EC:1.3.3.4)
    Short name:
    PPO2
    Alternative name(s):
    Protein MATERNAL EFFECT EMBRYO ARREST 61
    Gene namesi
    Name:PPOX2
    Synonyms:HEMG2, MEE61, PPO2
    Ordered Locus Names:At5g14220
    ORF Names:F18O22.10, MUA22.22
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G14220.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast envelope Source: TAIR
    3. mitochondrion Source: TAIR
    4. plastid Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Mitochondrion, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Embryo development arrested at one-cell zygotic stage.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2222Chloroplast and mitochondrionSequence AnalysisAdd
    BLAST
    Chaini23 – 508486Protoporphyrinogen oxidase 2, chloroplastic/mitochondrialPRO_0000422669Add
    BLAST

    Proteomic databases

    PRIDEiQ8S9J1.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8S9J1.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G14220.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8S9J1.
    SMRiQ8S9J1. Positions 16-499.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the protoporphyrinogen oxidase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1232.
    HOGENOMiHOG000269479.
    InParanoidiQ8S9J1.
    KOiK00231.
    OMAiISIYLGF.
    PhylomeDBiQ8S9J1.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.660.20. 1 hit.
    InterProiIPR002937. Amino_oxidase.
    IPR016040. NAD(P)-bd_dom.
    IPR027418. PPOX_C.
    IPR004572. Protoporphyrinogen_oxidase.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8S9J1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASGAVADHQ IEAVSGKRVA VVGAGVSGLA AAYKLKSRGL NVTVFEADGR    50
    VGGKLRSVMQ NGLIWDEGAN TMTEAEPEVG SLLDDLGLRE KQQFPISQKK 100
    RYIVRNGVPV MLPTNPIELV TSSVLSTQSK FQILLEPFLW KKKSSKVSDA 150
    SAEESVSEFF QRHFGQEVVD YLIDPFVGGT SAADPDSLSM KHSFPDLWNV 200
    EKSFGSIIVG AIRTKFAAKG GKSRDTKSSP GTKKGSRGSF SFKGGMQILP 250
    DTLCKSLSHD EINLDSKVLS LSYNSGSRQE NWSLSCVSHN ETQRQNPHYD 300
    AVIMTAPLCN VKEMKVMKGG QPFQLNFLPE INYMPLSVLI TTFTKEKVKR 350
    PLEGFGVLIP SKEQKHGFKT LGTLFSSMMF PDRSPSDVHL YTTFIGGSRN 400
    QELAKASTDE LKQVVTSDLQ RLLGVEGEPV SVNHYYWRKA FPLYDSSYDS 450
    VMEAIDKMEN DLPGFFYAGN HRGGLSVGKS IASGCKAADL VISYLESCSN 500
    DKKPNDSL 508
    Length:508
    Mass (Da):55,630
    Last modified:June 1, 2002 - v1
    Checksum:iF092957F1F3DD268
    GO
    Isoform 2 (identifier: Q8S9J1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         112-141: Missing.

    Show »
    Length:478
    Mass (Da):52,218
    Checksum:iEEB49AD0E4D8F129
    GO

    Sequence cautioni

    The sequence BAB08301.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAB87761.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei112 – 14130Missing in isoform 2. CuratedVSP_046550Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB007650 Genomic DNA. Translation: BAB08301.1. Sequence problems.
    AL163817 Genomic DNA. Translation: CAB87761.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED92001.1.
    CP002688 Genomic DNA. Translation: AED92002.1.
    AY075665 mRNA. Translation: AAL77672.1.
    AY101523 mRNA. Translation: AAM26644.1.
    PIRiT48595.
    RefSeqiNP_001190307.1. NM_001203378.1. [Q8S9J1-2]
    NP_196926.2. NM_121426.4. [Q8S9J1-1]
    UniGeneiAt.20436.

    Genome annotation databases

    EnsemblPlantsiAT5G14220.1; AT5G14220.1; AT5G14220. [Q8S9J1-1]
    GeneIDi831272.
    KEGGiath:AT5G14220.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB007650 Genomic DNA. Translation: BAB08301.1 . Sequence problems.
    AL163817 Genomic DNA. Translation: CAB87761.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED92001.1 .
    CP002688 Genomic DNA. Translation: AED92002.1 .
    AY075665 mRNA. Translation: AAL77672.1 .
    AY101523 mRNA. Translation: AAM26644.1 .
    PIRi T48595.
    RefSeqi NP_001190307.1. NM_001203378.1. [Q8S9J1-2 ]
    NP_196926.2. NM_121426.4. [Q8S9J1-1 ]
    UniGenei At.20436.

    3D structure databases

    ProteinModelPortali Q8S9J1.
    SMRi Q8S9J1. Positions 16-499.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G14220.1-P.

    Proteomic databases

    PRIDEi Q8S9J1.

    Protocols and materials databases

    DNASUi 831272.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G14220.1 ; AT5G14220.1 ; AT5G14220 . [Q8S9J1-1 ]
    GeneIDi 831272.
    KEGGi ath:AT5G14220.

    Organism-specific databases

    TAIRi AT5G14220.

    Phylogenomic databases

    eggNOGi COG1232.
    HOGENOMi HOG000269479.
    InParanoidi Q8S9J1.
    KOi K00231.
    OMAi ISIYLGF.
    PhylomeDBi Q8S9J1.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00324 .
    UPA00668 .
    BioCyci ARA:AT5G14220-MONOMER.
    ARA:GQT-1544-MONOMER.

    Gene expression databases

    Genevestigatori Q8S9J1.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.660.20. 1 hit.
    InterProi IPR002937. Amino_oxidase.
    IPR016040. NAD(P)-bd_dom.
    IPR027418. PPOX_C.
    IPR004572. Protoporphyrinogen_oxidase.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00562. proto_IX_ox. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
      Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis."
      Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S., Capron A., Xie L.-F., Ye D., Sundaresan V.
      Development 132:603-614(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    6. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
      Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
      PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
    7. "Defining the protein complex proteome of plant mitochondria."
      Klodmann J., Senkler M., Rode C., Braun H.-P.
      Plant Physiol. 157:587-598(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
    8. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPPOCM_ARATH
    AccessioniPrimary (citable) accession number: Q8S9J1
    Secondary accession number(s): F4K5H8, Q9FMS9, Q9LYA7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 26, 2013
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3