Q8S9F2 (PCYAA_EUGGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Photoactivated adenylate cyclase subunit alpha
EC=4.6.1.1

Alternative name(s):
Photoactivated adenylyl cyclase subunit alpha

Gene names

Name:

pacA

Organism

Euglena gracilis

Taxonomic identifier

3039 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Euglenida › Euglenales › Euglena

Protein attributes

Sequence length	1019 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a blue-light photoreceptor for the step-up photophobic response. Mediates photoavoidance. Ref.1
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate. Ref.1
Cofactor	FAD. Ref.1
Enzyme regulation	Activity increased by up to 80-fold under blue-light. Ref.1
Subunit structure	Heterotetramer of two alpha and two beta subunits. Ref.1
Subcellular location	Cell projection › cilium › flagellum. Note: Paraflagellar body, flagellum and paraxonemal bodies (PABs). Ref.1 Ref.2
Miscellaneous	The 1224-5/9F strain is deficient in phototaxis. It is not known if this is due to defective adenylate cyclase activity or defective BLUF domains in the beta subunit of this protein. Ref.2
Sequence similarities	Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 2 BLUF domains. Contains 2 guanylate cyclase domains.
Biophysicochemical properties	Kinetic parameters: KM=0.5 µM for ATP Ref.1 Vmax=3.5 nmol/min/mg enzyme Ref.1

Ontologies

Keywords
Biological process	Sensory transduction cAMP biosynthesis
Cellular component	Cell projection Cilium Flagellum
Domain	Repeat
Ligand	ATP-binding Chromophore FAD Flavoprotein Nucleotide-binding
Molecular function	Lyase Photoreceptor protein Receptor
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cilium Inferred from electronic annotation. Source: UniProtKB-KW flagellum Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Inferred from direct assay Ref.1. Source: UniProtKB photoreceptor activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1019	1019	Photoactivated adenylate cyclase subunit alpha		PRO_0000195717
Regions
	Domain	55 – 148	94	BLUF 1
	Domain	204 – 332	129	Guanylate cyclase 1
	Domain	467 – 559	93	BLUF 2
	Domain	615 – 744	130	Guanylate cyclase 2

Sequences

	Sequence		Length	Mass (Da)
	Q8S9F2 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: C28F8B3D1EAB1F34	FASTA	1,019	111,990
	10 20 30 40 50 60 MYILVWKEGQ QIRTFQDLEE CGQFQTASNI TDGQIFSINV TPTMSKGGET GETQLRRLMY 70 80 90 100 110 120 LSASTEPEKC NAEYLADMAH VATLRNKQIG VSGFLLYSSP FFFQVIEGTD EDLDFLFAKI 130 140 150 160 170 180 SADPRHERCI VLANGPCTGR MYGEWHMKDS HIDNITKHPA IKTILFQIAR SFSSMWSYLP 190 200 210 220 230 240 KNAANMLLLG KNPNKQAPEP MSVVVTFIYL VEFSSILAHP GLTEQCADIL AAFVDACVRN 250 260 270 280 290 300 VEGTGGQVAK FITGICMAYW PINRAEDALV GLQQLSEDLA ELRSQQPPGS ALSLIYSRCG 310 320 330 340 350 360 VHYGRALLCN AGFRKADFTL LGDCINTASR ITSLSVKLKV PLLLSFEVRC LLGDEMREEL 370 380 390 400 410 420 ESSGLHKVKG RDKPVQVYQF NAPELDSAMV RAKIEQFNPG RYRALCPVKP YESLHPAQRP 430 440 450 460 470 480 PIFDDTPREN QPKLSQVQRR DSLVDRLSLI AKLAFPSSMM AGGEGQLITL TYISQAAHPM 490 500 510 520 530 540 SRLDLASIQR IAFARNESSN ITGSLLYVSG LFVQTLEGPK GAVVSLYLKI RQDKRHKDVV 550 560 570 580 590 600 AVFMAPIDER VYGSPLDMTS ATEEMLATFP PLQDVLSQLA KSFISLETYV PSTVVRYLTA 610 620 630 640 650 660 GNNPRNLQPV SVEVVMLATD ICSFTPLSEK CSLTEVWTIC NTFIDACTSA ICNEGGEVIK 670 680 690 700 710 720 LIGDCVTAYF PPTGADNAVH ACQEIVSFCA QLRDAFHDVL DCRSVVACGV GLDFGQVIMA 730 740 750 760 770 780 QCGSLGMTEF VVAGEVSARV MEVEALTREA GRAIVITEPV ADRLSPKLRD TGIVPCQEGV 790 800 810 820 830 840 DGVPCYGILG PEWELDVATI KKNIYGFHDA RALAAMKKVD DGTNAPGRGA PAGGIPSSPK 850 860 870 880 890 900 VRPPGRTNSV SSYTPDPNEA LDPRMAESVF LDMCHQRGDT ANNSIAVKLR QAANDDRLDL 910 920 930 940 950 960 GRMLQGPHEL MPVMQAIKHL TNLRMLNMSD NFVDDNNVGE LVESCIPMRS LQVLDLSNNP 970 980 990 1000 1010 GLTKVIALKR LIKHNTQVRE ILLNGTRIAP TEQRKLQSSM NVNRLCASTD LKGSHKYEH

References

[1]

"A blue-light-activated adenylyl cyclase mediates photoavoidance in Euglena gracilis." Iseki M., Matsunaga S., Murakami A., Ohno K., Shiga K., Yoshida K., Sugai M., Takahashi T., Hori T., Watanabe M. Nature 415:1047-1051(2002) [PubMed: 11875575] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-31, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION. Strain: Z / UTEX 753.

[2]

"Photoactivated adenylyl cyclase (PAC) genes in the flagellate Euglena gracilis mutant strains." Ntefidou M., Haeder D.-P. Photochem. Photobiol. Sci. 4:732-739(2005) [PubMed: 16121285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION. Strain: SAG 1224-5/9F.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB031225 mRNA. Translation: BAB85619.1. AM181334 mRNA. Translation: CAJ57393.1.
3D structure databases
ProteinModelPortal	Q8S9F2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001054. A/G_cyclase. IPR001792. Acylphosphatase-like. IPR007024. BLUF_domain. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 2 hits.
Pfam	PF04940. BLUF. 2 hits. PF00211. Guanylate_cyc. 2 hits. [Graphical view]
SMART	SM01034. BLUF. 2 hits. SM00044. CYCc. 1 hit. [Graphical view]
SUPFAM	SSF55073. A/G_cyclase. 2 hits. SSF54975. Acylphosphatase. 2 hits.
PROSITE	PS50925. BLUF. 2 hits. PS00452. GUANYLATE_CYCLASE_1. False negative. PS50125. GUANYLATE_CYCLASE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCYAA_EUGGR

Accession

Primary (citable) accession number: Q8S9F2
Secondary accession number(s): Q2P9N1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)