Reviewed, UniProtKB/Swiss-Prot Q8S9F1 (PCYAB_EUGGR)

Names and origin

Protein names

Recommended name:
    Photoactivated adenylate cyclase subunit beta
    EC=4.6.1.1
Alternative name(s):
    Photoactivated adenylyl cyclase subunit beta

Gene names

Name:

pacB

Organism

Euglena gracilis

Taxonomic identifier

3039 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Euglenida › Euglenales › Euglena

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a blue-light photoreceptor for the step-up photophobic response. Mediates photoavoidance. Ref.1
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate. Ref.1
Cofactor	FAD. Ref.1
Enzyme regulation	Activity increased by up to 80-fold under blue-light. Ref.1
Subunit structure	Heterotetramer of two alpha and two beta subunits. Ref.1
Subcellular location	Cell projection › cilium › flagellum. Note: Paraflagellar body, flagellum and paraxonemal bodies (PABs). Ref.1 Ref.2
Sequence similarities	Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 2 BLUF domains. Contains 2 guanylate cyclase domains.
Biophysicochemical properties	Kinetic parameters: KM=0.5 µM for ATP Ref.1 Vmax=3.5 nmol/min/mg enzyme Ref.1

Ontologies

Keywords
Biological process	Sensory transduction cAMP biosynthesis
Cellular component	Cell projection Cilium Flagellum
Domain	Repeat
Ligand	ATP-binding Chromophore FAD Flavoprotein Nucleotide-binding
Molecular function	Lyase Photoreceptor protein Receptor
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cAMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW intracellular signaling cascade Inferred from electronic annotation. Source: InterPro protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cilium Inferred from electronic annotation. Source: UniProtKB-KW flagellum Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Ref.1 Inferred from direct assay. Source: UniProtKB photoreceptor activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 859	859	Photoactivated adenylate cyclase subunit beta		PRO_0000195718
Regions
	Domain	56 – 149	94	BLUF 1
	Domain	205 – 333	129	Guanylate cyclase 1
	Domain	471 – 563	93	BLUF 2
	Domain	619 – 748	130	Guanylate cyclase 2

Sequences

	Sequence		Length	Mass (Da)
	Q8S9F1-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: C0EF4406D4A8F61E	FASTA	859	94,221
	10 20 30 40 50 60 MYILVWKKGQ QIKTFHTLDE AAQFKAASNI DEAQMFSVTV APAISASGGS NEATNLRRLM 70 80 90 100 110 120 YLSKSTNPEE CNPQFLAEMA RVATIRNREI GVSGFLMYSS PFFFQVIEGT DEDLDFLFAK 130 140 150 160 170 180 ISADPRHERC IVLANGPCTG RMYGDWHMKD SHMDSITTHP AMKTILYQIA RSFSSMWSYL 190 200 210 220 230 240 PKSAGNMLLL GKDPAAQPPE PMSVVVTFIY LVEFGSILSN PNLTEQAAEV LSTFVDVCVK 250 260 270 280 290 300 NVEGSGGNIA KFITGICMAY WPINRTEEAL TAIQQISEDL AQLRSQQAPG SAVSLMYSQA 310 320 330 340 350 360 GVHYGRAMLC NAGSRKSDFT LLGDCINTTS RIATLAKKLK TPLLFSFEVR CLLGDEMREE 370 380 390 400 410 420 IEGAGMHQVK GRDKPVVVYQ FPGPELDVEM VRQKIEQFTP GRFRCQMPVV EYEALPISQR 430 440 450 460 470 480 PPIFDDTPKG NPRPRTPGYG GRQRSDSLVD RLIMIAKLAG PSVSATGDTT LTTLTYISQA 490 500 510 520 530 540 TRPMSRLDLS AIMRTATRRN AQQSITGTLL HVNGLFVQTL EGPKDAVVNL YLRIRQDPRH 550 560 570 580 590 600 TDVTTVHMAP LQERVYPSEW TLTSATEEML ATFPPLQDVL SQLAKSFTSL ETYVPSTVVR 610 620 630 640 650 660 YLTAGNNPRN LMPVSCGVVM LATDICSFTS LTEKSSLTEV WMICNTFIDA CTSAICQEGG 670 680 690 700 710 720 EVIKLIGDCV TAYFPGNNAD SAVAAAQELF TFCRQLREAF VDVLDVRGCV SCGVGLDYGQ 730 740 750 760 770 780 VVMAQCGSLG LTEYVVAGAV SARVMEVEAI TREVGYAIVV TEPVADRLSP QLRDHGIVPT 790 800 810 820 830 840 PQAIEGLPCY GIAGEEFELD VDSIKRGIKA LHAARSGEKP LTEPEEAKPD FRVSPGRVRH 850 GDSGRRSNSA QGKRSIQVR

References

[1]

"A blue-light-activated adenylyl cyclase mediates photoavoidance in Euglena gracilis." Iseki M., Matsunaga S., Murakami A., Ohno K., Shiga K., Yoshida K., Sugai M., Takahashi T., Hori T., Watanabe M. Nature 415:1047-1051(2002) [PubMed: 11875575] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-32, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION. Strain: Z / UTEX 753.

[2]

"Photoactivated adenylyl cyclase (PAC) genes in the flagellate Euglena gracilis mutant strains." Ntefidou M., Haeder D.-P. Photochem. Photobiol. Sci. 4:732-739(2005) [PubMed: 16121285] [Abstract] Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB031226 mRNA. Translation: BAB85620.1.
3D structure databases
SMR	Q8S9F1. Positions 53-150, 56-180, 203-381, 470-599, 618-772.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.6.1.1. 260.
Family and domain databases
InterPro	IPR001054. A/G_cyclase. IPR007024. BLUF. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 2 hits.
Pfam	PF04940. BLUF. 2 hits. PF00211. Guanylate_cyc. 2 hits. [Graphical view]
SMART	SM00044. CYCc. 1 hit. [Graphical view]
PROSITE	PS50925. BLUF. 2 hits. PS00452. GUANYLATE_CYCLASE_1. False negative. PS50125. GUANYLATE_CYCLASE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCYAB_EUGGR

Accession

Primary (citable) accession number: Q8S9F1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	June 1, 2002
Last modified:	January 19, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)