Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8S920

- UBC5A_ORYSJ

UniProt

Q8S920 - UBC5A_ORYSJ

Protein

Ubiquitin-conjugating enzyme E2 5A

Gene

UBC5A

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    E2 conjugating enzyme that associates with the E3 ubiquitin-protein ligase EL5 to mediates ubiquitination of target proteins.1 Publication

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_204392. APC/C:Cdc20 mediated degradation of mitotic proteins.
    REACT_207219. APC/C:Cdc20 mediated degradation of Securin.
    REACT_212204. Separation of Sister Chromatids.
    REACT_217276. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_221147. Senescence-Associated Secretory Phenotype (SASP).
    REACT_225596. APC/C:Cdc20 mediated degradation of Cyclin B.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 5A (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 5a
    Short name:
    OsUBC5a
    Ubiquitin-protein ligase 5A
    Gene namesi
    Name:UBC5A
    Ordered Locus Names:Os01g0658400, LOC_Os01g46926
    ORF Names:OsJ_02884
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 1

    Organism-specific databases

    GrameneiQ8S920.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 147147Ubiquitin-conjugating enzyme E2 5APRO_0000397684Add
    BLAST

    Proteomic databases

    PaxDbiQ8S920.
    PRIDEiQ8S920.

    Interactioni

    Protein-protein interaction databases

    STRINGi39946.BGIOSIBCE003038.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8S920.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    KOiK06689.
    OMAiLAQEWTT.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8S920-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASKRIQKEL KDLQKDPPTS CSAGPVGEDM FHWQATIMGP SDSPYAGGVF    50
    LVTIHFPPDY PFKPPKVAFR TKVFHPNINS NGSICLDILK DQWSPALTIS 100
    KVLLSICSLL TDPNPDDPLV PEIAHMYKTD RHKYENTART WTQRYAM 147
    Length:147
    Mass (Da):16,573
    Last modified:June 1, 2002 - v1
    Checksum:i9FAA726F5C506F09
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB074411 mRNA. Translation: BAB89354.1.
    AP008207 Genomic DNA. Translation: BAF05677.1.
    CM000138 Genomic DNA. Translation: EEE55114.1.
    AK063826 mRNA. Translation: BAG88879.1.
    AK099284 mRNA. Translation: BAG94041.1.
    RefSeqiNP_001043763.1. NM_001050298.1.
    UniGeneiOs.8873.

    Genome annotation databases

    EnsemblPlantsiOS01T0658400-01; OS01T0658400-01; OS01G0658400.
    GeneIDi4327162.
    KEGGiosa:4327162.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB074411 mRNA. Translation: BAB89354.1 .
    AP008207 Genomic DNA. Translation: BAF05677.1 .
    CM000138 Genomic DNA. Translation: EEE55114.1 .
    AK063826 mRNA. Translation: BAG88879.1 .
    AK099284 mRNA. Translation: BAG94041.1 .
    RefSeqi NP_001043763.1. NM_001050298.1.
    UniGenei Os.8873.

    3D structure databases

    ProteinModelPortali Q8S920.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 39946.BGIOSIBCE003038.

    Proteomic databases

    PaxDbi Q8S920.
    PRIDEi Q8S920.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS01T0658400-01 ; OS01T0658400-01 ; OS01G0658400 .
    GeneIDi 4327162.
    KEGGi osa:4327162.

    Organism-specific databases

    Gramenei Q8S920.

    Phylogenomic databases

    eggNOGi COG5078.
    KOi K06689.
    OMAi LAQEWTT.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_204392. APC/C:Cdc20 mediated degradation of mitotic proteins.
    REACT_207219. APC/C:Cdc20 mediated degradation of Securin.
    REACT_212204. Separation of Sister Chromatids.
    REACT_217276. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_221147. Senescence-Associated Secretory Phenotype (SASP).
    REACT_225596. APC/C:Cdc20 mediated degradation of Cyclin B.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "EL5, a rice N-acetylchitooligosaccharide elicitor-responsive RING-H2 finger protein, is a ubiquitin ligase which functions in vitro in co-operation with an elicitor-responsive ubiquitin-conjugating enzyme, OsUBC5b."
      Takai R., Matsuda N., Nakano A., Hasegawa K., Akimoto C., Shibuya N., Minami E.
      Plant J. 30:447-455(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
      Strain: cv. Nipponbare.
    2. "The genome sequence and structure of rice chromosome 1."
      Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
      , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
      Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    5. "The genomes of Oryza sativa: a history of duplications."
      Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
      , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
      PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    6. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiUBC5A_ORYSJ
    AccessioniPrimary (citable) accession number: Q8S920
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3