UBC5A

Functionⁱ

E2 conjugating enzyme that associates with the E3 ubiquitin-protein ligase EL5 to mediates ubiquitination of target proteins.1 Publication

Catalytic activityⁱ

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	85	Glycyl thioester intermediatePROSITE-ProRule annotation		1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
ubiquitin-protein transferase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 12028574

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

protein ubiquitination
Source: UniProtKB
Inferred from direct assayⁱ
- 12028574

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Ubl conjugation pathway
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-OSA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A. R-OSA-5689896. Ovarian tumor domain proteases. R-OSA-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes. R-OSA-8866654. E3 ubiquitin ligases ubiquitinate target proteins. R-OSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayⁱ	UPA00143.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ubiquitin-conjugating enzyme E2 5A (EC:2.3.2.23) Alternative name(s): E2 ubiquitin-conjugating enzyme 5A Ubiquitin carrier protein 5a Short name: OsUBC5a Ubiquitin-protein ligase 5A
Gene namesⁱ	Name:UBC5A Ordered Locus Names:Os01g0658400, LOC_Os01g46926 ORF Names:OsJ_02884
Organismⁱ	Oryza sativa subsp. japonica (Rice)
Taxonomic identifierⁱ	39947 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › Liliopsida › Petrosaviidae › commelinids › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa
Proteomesⁱ	UP000059680 Componentⁱ: Chromosome 1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000397684	1 – 147	Ubiquitin-conjugating enzyme E2 5AAdd BLAST		147

Proteomic databases

PaxDbⁱ	Q8S920.
PRIDEⁱ	Q8S920.

Expressionⁱ

Gene expression databases

ExpressionAtlasⁱ	Q8S920. baseline and differential.
Genevisibleⁱ	Q8S920. OS.

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	39947.LOC_Os01g46926.1.

STRINGⁱ

39947.LOC_Os01g46926.1.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8S920.
SMRⁱ	Q8S920.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGⁱ	KOG0417. Eukaryota. COG5078. LUCA.
InParanoidⁱ	Q8S920.
KEGG Orthology (KO) More...KOⁱ	K06689.
OMAⁱ	TDRQKYN.
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360PU6.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00195. UBCc. 1 hit.
Gene3Dⁱ	3.10.110.10. 1 hit.
InterProⁱ	View protein in InterPro IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00179. UQ_con. 1 hit.
SUPFAMⁱ	SSF54495. SSF54495. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q8S920-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MASKRIQKEL KDLQKDPPTS CSAGPVGEDM FHWQATIMGP SDSPYAGGVF 
        60         70         80         90        100
LVTIHFPPDY PFKPPKVAFR TKVFHPNINS NGSICLDILK DQWSPALTIS 
       110        120        130        140 
KVLLSICSLL TDPNPDDPLV PEIAHMYKTD RHKYENTART WTQRYAM

Length:147

Mass (Da):16,573

Last modified:June 1, 2002 - v1

Checksum:ⁱ9FAA726F5C506F09

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB074411 mRNA. Translation: BAB89354.1. AP008207 Genomic DNA. Translation: BAF05677.1. AP014957 Genomic DNA. Translation: BAS73515.1. CM000138 Genomic DNA. Translation: EEE55114.1. AK063826 mRNA. Translation: BAG88879.1. AK099284 mRNA. Translation: BAG94041.1.
NCBI Reference Sequences More...RefSeqⁱ	XP_015650522.1. XM_015795036.1.
UniGeneⁱ	Os.8873.

Genome annotation databases

EnsemblPlantsⁱ	Os01t0658400-01; Os01t0658400-01; Os01g0658400.
GeneIDⁱ	4327162.
Grameneⁱ	Os01t0658400-01; Os01t0658400-01; Os01g0658400.
KEGGⁱ	osa:4327162.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q8S920	I1NQB6 B8A6V8 A0A0E0N078	Oryza glaberrima (African rice) Oryza sativa subsp. indica (Rice) Oryza rufipogon (Brownbeard rice) (Asian wild rice)	147	UniRef100_Q8S920	Cluster: Ubiquitin-conjugating enzyme E2 5A	4

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q8S920	I1NQB6 B8A6V8 A0A0E0N078 A0A0E0FQG9	Oryza glaberrima (African rice) Oryza sativa subsp. indica (Rice) Oryza rufipogon (Brownbeard rice) (Asian wild rice) Oryza nivara (Indian wild rice)	147	UniRef90_Q8S920	Cluster: Ubiquitin-conjugating enzyme E2 5A	5

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q8S920	I1NQB6 B8A6V8 A0A0E0N078 A0A0E0FQG9 A0A0E0N079 A0A2C9VSR8 UPI000B5D2FF2 UPI000B5D6F48 UPI000B5D59CC A0A0E0JQC2 +14	Oryza glaberrima (African rice) Oryza sativa subsp. indica (Rice) Oryza rufipogon (Brownbeard rice) (Asian wild rice) Oryza nivara (Indian wild rice) Manihot esculenta (Cassava) (Jatropha manihot) Oryza punctata (Red rice) Oryza sativa subsp. japonica (Rice) Oryza brachyantha Leersia perrieri Oryza meridionalis And more	147	UniRef50_Q8S920	Cluster: Ubiquitin-conjugating enzyme E2 5A	25

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB074411 mRNA. Translation: BAB89354.1. AP008207 Genomic DNA. Translation: BAF05677.1. AP014957 Genomic DNA. Translation: BAS73515.1. CM000138 Genomic DNA. Translation: EEE55114.1. AK063826 mRNA. Translation: BAG88879.1. AK099284 mRNA. Translation: BAG94041.1.
NCBI Reference Sequences More...RefSeqⁱ	XP_015650522.1. XM_015795036.1.
UniGeneⁱ	Os.8873.

3D structure databases

ProteinModelPortalⁱ	Q8S920.
SMRⁱ	Q8S920.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	39947.LOC_Os01g46926.1.

STRINGⁱ

39947.LOC_Os01g46926.1.

Proteomic databases

PaxDbⁱ	Q8S920.
PRIDEⁱ	Q8S920.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	Os01t0658400-01; Os01t0658400-01; Os01g0658400.
GeneIDⁱ	4327162.
Grameneⁱ	Os01t0658400-01; Os01t0658400-01; Os01g0658400.
KEGGⁱ	osa:4327162.

Phylogenomic databases

eggNOGⁱ	KOG0417. Eukaryota. COG5078. LUCA.
InParanoidⁱ	Q8S920.
KEGG Orthology (KO) More...KOⁱ	K06689.
OMAⁱ	TDRQKYN.
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360PU6.

Enzyme and pathway databases

UniPathwayⁱ	UPA00143.
Reactomeⁱ	R-OSA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A. R-OSA-5689896. Ovarian tumor domain proteases. R-OSA-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes. R-OSA-8866654. E3 ubiquitin ligases ubiquitinate target proteins. R-OSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Gene expression databases

ExpressionAtlasⁱ	Q8S920. baseline and differential.
Genevisibleⁱ	Q8S920. OS.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00195. UBCc. 1 hit.
Gene3Dⁱ	3.10.110.10. 1 hit.
InterProⁱ	View protein in InterPro IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00179. UQ_con. 1 hit.
SUPFAMⁱ	SSF54495. SSF54495. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	UBC5A_ORYSJ
Accessionⁱ	Q8S920Primary (citable) accession number: Q8S920 Secondary accession number(s): A0A0P0V661
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
	Last sequence update:	June 1, 2002
	Last modified:	February 28, 2018
	This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q8S920 (UBC5A_ORYSJ)

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Ubiquitin-conjugating enzyme E2 5A

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ