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Q8S919

- UBC5B_ORYSJ

UniProt

Q8S919 - UBC5B_ORYSJ

Protein

Ubiquitin-conjugating enzyme E2 5B

Gene

UBC5B

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    E2 conjugating enzyme that associates with the E3 ubiquitin-protein ligase EL5 to mediates ubiquitination of target proteins.1 Publication

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB
    2. ubiquitin-dependent protein catabolic process Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_204392. APC/C:Cdc20 mediated degradation of mitotic proteins.
    REACT_207219. APC/C:Cdc20 mediated degradation of Securin.
    REACT_212204. Separation of Sister Chromatids.
    REACT_217276. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_221147. Senescence-Associated Secretory Phenotype (SASP).
    REACT_225596. APC/C:Cdc20 mediated degradation of Cyclin B.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 5B (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 5b
    Short name:
    OsUBC5b
    Ubiquitin-protein ligase 5B
    Gene namesi
    Name:UBC5B
    Ordered Locus Names:Os02g0261100, LOC_Os02g16040
    ORF Names:OsJ_06152, OSJNBb0026F09.5
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 2

    Organism-specific databases

    GrameneiQ8S919.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: EnsemblPlants/Gramene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 148148Ubiquitin-conjugating enzyme E2 5BPRO_0000397685Add
    BLAST

    Proteomic databases

    PaxDbiQ8S919.
    PRIDEiQ8S919.

    Expressioni

    Inductioni

    By N-acetylchitooligosaccharide elicitor.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ8S919.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    KOiK06689.
    OMAiNTHAREW.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8S919-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATLMGP SDSPYAGGVF    50
    LVTIHFPPDY PFKPPKVALK TKVFHPNINS NGSICLDILK EQWSPALTIS 100
    KVLLSICSLL TDPNPDDPLV PEIAHMYKTD RAKYESTARS WTQKYAMG 148
    Length:148
    Mass (Da):16,446
    Last modified:June 1, 2002 - v1
    Checksum:i8BD25EF09F70D1DD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB074412 mRNA. Translation: BAB89355.1.
    AP005777 Genomic DNA. Translation: BAD20047.1.
    AP008208 Genomic DNA. Translation: BAF08400.1.
    CM000139 Genomic DNA. Translation: EAZ22486.1.
    AK121248 mRNA. Translation: BAH00395.1.
    RefSeqiNP_001046486.1. NM_001053021.1.
    UniGeneiOs.7899.

    Genome annotation databases

    EnsemblPlantsiOS02T0261100-01; OS02T0261100-01; OS02G0261100.
    GeneIDi4328940.
    KEGGiosa:4328940.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB074412 mRNA. Translation: BAB89355.1 .
    AP005777 Genomic DNA. Translation: BAD20047.1 .
    AP008208 Genomic DNA. Translation: BAF08400.1 .
    CM000139 Genomic DNA. Translation: EAZ22486.1 .
    AK121248 mRNA. Translation: BAH00395.1 .
    RefSeqi NP_001046486.1. NM_001053021.1.
    UniGenei Os.7899.

    3D structure databases

    ProteinModelPortali Q8S919.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q8S919.
    PRIDEi Q8S919.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS02T0261100-01 ; OS02T0261100-01 ; OS02G0261100 .
    GeneIDi 4328940.
    KEGGi osa:4328940.

    Organism-specific databases

    Gramenei Q8S919.

    Phylogenomic databases

    eggNOGi COG5078.
    KOi K06689.
    OMAi NTHAREW.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_204392. APC/C:Cdc20 mediated degradation of mitotic proteins.
    REACT_207219. APC/C:Cdc20 mediated degradation of Securin.
    REACT_212204. Separation of Sister Chromatids.
    REACT_217276. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_221147. Senescence-Associated Secretory Phenotype (SASP).
    REACT_225596. APC/C:Cdc20 mediated degradation of Cyclin B.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "EL5, a rice N-acetylchitooligosaccharide elicitor-responsive RING-H2 finger protein, is a ubiquitin ligase which functions in vitro in co-operation with an elicitor-responsive ubiquitin-conjugating enzyme, OsUBC5b."
      Takai R., Matsuda N., Nakano A., Hasegawa K., Akimoto C., Shibuya N., Minami E.
      Plant J. 30:447-455(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
      Strain: cv. Nipponbare.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    4. "The genomes of Oryza sativa: a history of duplications."
      Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
      , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
      PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiUBC5B_ORYSJ
    AccessioniPrimary (citable) accession number: Q8S919
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3