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Protein

Ubiquitin-conjugating enzyme E2 5B

Gene

UBC5B

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

E2 conjugating enzyme that associates with the E3 ubiquitin-protein ligase EL5 to mediates ubiquitination of target proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_204392. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_207219. APC/C:Cdc20 mediated degradation of Securin.
REACT_212204. Separation of Sister Chromatids.
REACT_217276. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_221147. Senescence-Associated Secretory Phenotype (SASP).
REACT_225596. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_229639. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_240160. Regulation of APC/C activators between G1/S and early anaphase.
REACT_243301. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_255187. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 5B (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 5b
Short name:
OsUBC5b
Ubiquitin-protein ligase 5B
Gene namesi
Name:UBC5B
Ordered Locus Names:Os02g0261100, LOC_Os02g16040
ORF Names:OsJ_06152, OSJNBb0026F09.5
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 2

Organism-specific databases

GrameneiQ8S919.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 148148Ubiquitin-conjugating enzyme E2 5BPRO_0000397685Add
BLAST

Proteomic databases

PaxDbiQ8S919.
PRIDEiQ8S919.

Expressioni

Inductioni

By N-acetylchitooligosaccharide elicitor.1 Publication

Gene expression databases

ExpressionAtlasiQ8S919. baseline.

Structurei

3D structure databases

ProteinModelPortaliQ8S919.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
InParanoidiQ8S919.
KOiK06689.
OMAiINGSICL.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8S919-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATLMGP SDSPYAGGVF
60 70 80 90 100
LVTIHFPPDY PFKPPKVALK TKVFHPNINS NGSICLDILK EQWSPALTIS
110 120 130 140
KVLLSICSLL TDPNPDDPLV PEIAHMYKTD RAKYESTARS WTQKYAMG
Length:148
Mass (Da):16,446
Last modified:June 1, 2002 - v1
Checksum:i8BD25EF09F70D1DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB074412 mRNA. Translation: BAB89355.1.
AP005777 Genomic DNA. Translation: BAD20047.1.
AP008208 Genomic DNA. Translation: BAF08400.1.
CM000139 Genomic DNA. Translation: EAZ22486.1.
AK121248 mRNA. Translation: BAH00395.1.
RefSeqiNP_001046486.1. NM_001053021.1.
UniGeneiOs.7899.

Genome annotation databases

EnsemblPlantsiOS02T0261100-01; OS02T0261100-01; OS02G0261100.
GeneIDi4328940.
KEGGiosa:4328940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB074412 mRNA. Translation: BAB89355.1.
AP005777 Genomic DNA. Translation: BAD20047.1.
AP008208 Genomic DNA. Translation: BAF08400.1.
CM000139 Genomic DNA. Translation: EAZ22486.1.
AK121248 mRNA. Translation: BAH00395.1.
RefSeqiNP_001046486.1. NM_001053021.1.
UniGeneiOs.7899.

3D structure databases

ProteinModelPortaliQ8S919.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ8S919.
PRIDEiQ8S919.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS02T0261100-01; OS02T0261100-01; OS02G0261100.
GeneIDi4328940.
KEGGiosa:4328940.

Organism-specific databases

GrameneiQ8S919.

Phylogenomic databases

eggNOGiCOG5078.
InParanoidiQ8S919.
KOiK06689.
OMAiINGSICL.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiREACT_204392. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_207219. APC/C:Cdc20 mediated degradation of Securin.
REACT_212204. Separation of Sister Chromatids.
REACT_217276. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_221147. Senescence-Associated Secretory Phenotype (SASP).
REACT_225596. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_229639. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_240160. Regulation of APC/C activators between G1/S and early anaphase.
REACT_243301. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_255187. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.

Gene expression databases

ExpressionAtlasiQ8S919. baseline.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "EL5, a rice N-acetylchitooligosaccharide elicitor-responsive RING-H2 finger protein, is a ubiquitin ligase which functions in vitro in co-operation with an elicitor-responsive ubiquitin-conjugating enzyme, OsUBC5b."
    Takai R., Matsuda N., Nakano A., Hasegawa K., Akimoto C., Shibuya N., Minami E.
    Plant J. 30:447-455(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiUBC5B_ORYSJ
AccessioniPrimary (citable) accession number: Q8S919
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2002
Last modified: March 4, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.