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Q8S919

- UBC5B_ORYSJ

UniProt

Q8S919 - UBC5B_ORYSJ

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Protein

Ubiquitin-conjugating enzyme E2 5B

Gene
UBC5B, Os02g0261100, LOC_Os02g16040, OsJ_06152, OSJNBb0026F09.5
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

E2 conjugating enzyme that associates with the E3 ubiquitin-protein ligase EL5 to mediates ubiquitination of target proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. protein ubiquitination Source: UniProtKB
  2. ubiquitin-dependent protein catabolic process Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_204392. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_207219. APC/C:Cdc20 mediated degradation of Securin.
REACT_212204. Separation of Sister Chromatids.
REACT_217276. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_221147. Senescence-Associated Secretory Phenotype (SASP).
REACT_225596. APC/C:Cdc20 mediated degradation of Cyclin B.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 5B (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 5b
Short name:
OsUBC5b
Ubiquitin-protein ligase 5B
Gene namesi
Name:UBC5B
Ordered Locus Names:Os02g0261100, LOC_Os02g16040
ORF Names:OsJ_06152, OSJNBb0026F09.5
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 2

Organism-specific databases

GrameneiQ8S919.

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: EnsemblPlants/Gramene
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 148148Ubiquitin-conjugating enzyme E2 5BPRO_0000397685Add
BLAST

Proteomic databases

PaxDbiQ8S919.
PRIDEiQ8S919.

Expressioni

Inductioni

By N-acetylchitooligosaccharide elicitor.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8S919.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5078.
KOiK06689.
OMAiNTHAREW.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8S919-1 [UniParc]FASTAAdd to Basket

« Hide

MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATLMGP SDSPYAGGVF    50
LVTIHFPPDY PFKPPKVALK TKVFHPNINS NGSICLDILK EQWSPALTIS 100
KVLLSICSLL TDPNPDDPLV PEIAHMYKTD RAKYESTARS WTQKYAMG 148
Length:148
Mass (Da):16,446
Last modified:June 1, 2002 - v1
Checksum:i8BD25EF09F70D1DD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB074412 mRNA. Translation: BAB89355.1.
AP005777 Genomic DNA. Translation: BAD20047.1.
AP008208 Genomic DNA. Translation: BAF08400.1.
CM000139 Genomic DNA. Translation: EAZ22486.1.
AK121248 mRNA. Translation: BAH00395.1.
RefSeqiNP_001046486.1. NM_001053021.1.
UniGeneiOs.7899.

Genome annotation databases

EnsemblPlantsiOS02T0261100-01; OS02T0261100-01; OS02G0261100.
GeneIDi4328940.
KEGGiosa:4328940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB074412 mRNA. Translation: BAB89355.1 .
AP005777 Genomic DNA. Translation: BAD20047.1 .
AP008208 Genomic DNA. Translation: BAF08400.1 .
CM000139 Genomic DNA. Translation: EAZ22486.1 .
AK121248 mRNA. Translation: BAH00395.1 .
RefSeqi NP_001046486.1. NM_001053021.1.
UniGenei Os.7899.

3D structure databases

ProteinModelPortali Q8S919.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q8S919.
PRIDEi Q8S919.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS02T0261100-01 ; OS02T0261100-01 ; OS02G0261100 .
GeneIDi 4328940.
KEGGi osa:4328940.

Organism-specific databases

Gramenei Q8S919.

Phylogenomic databases

eggNOGi COG5078.
KOi K06689.
OMAi NTHAREW.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_204392. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_207219. APC/C:Cdc20 mediated degradation of Securin.
REACT_212204. Separation of Sister Chromatids.
REACT_217276. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_221147. Senescence-Associated Secretory Phenotype (SASP).
REACT_225596. APC/C:Cdc20 mediated degradation of Cyclin B.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "EL5, a rice N-acetylchitooligosaccharide elicitor-responsive RING-H2 finger protein, is a ubiquitin ligase which functions in vitro in co-operation with an elicitor-responsive ubiquitin-conjugating enzyme, OsUBC5b."
    Takai R., Matsuda N., Nakano A., Hasegawa K., Akimoto C., Shibuya N., Minami E.
    Plant J. 30:447-455(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiUBC5B_ORYSJ
AccessioniPrimary (citable) accession number: Q8S919
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2002
Last modified: September 3, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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