Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8S8S2 (LPCT2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysophospholipid acyltransferase LPEAT2

EC=2.3.1.25
Alternative name(s):
Lysophosphatidylethanolamine acyltransferase 2
Short name=AtLPEAT2
Gene names
Name:LPEAT2
Ordered Locus Names:At2g45670
ORF Names:F17K2.20
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length539 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Displays acyl-CoA-dependent lysophospholipid acyltransferase activity with a subset of lysophospholipids as substrates. Exhibits strong acylation activity of lysophosphatidylethanolamine (LPE) and lower activity on lysophosphatidate (LPA). Has a preference for 18:1-LPE over 16:0-LPE as acceptor and palmitoyl-CoA (16:0-CoA) as acyl donor. Activity is calcium-independent. Ref.6

Catalytic activity

Acyl-CoA + 1-O-(alk-1-enyl)glycero-3-phosphocholine = CoA + plasmenylcholine. Ref.6

Pathway

Lipid metabolism; phospholipid metabolism.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Domain

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphocholine By similarity.

Miscellaneous

The ratio of isoform 2/isoform1 mRNA is increased about 15-fold in the nonsense mRNA reducing factor mutant upf3-1 (Ref.5).

Sequence similarities

Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Contains 3 EF-hand domains.

Biophysicochemical properties

pH dependence:

Optimum pH is 9.0. Ref.6

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8S8S2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8S8S2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     236-281: RKASCDRFPR...YPIQPVVVRY → GCKYREKLPA...LSSLVTLFNL
     282-539: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 539539Lysophospholipid acyltransferase LPEAT2
PRO_0000422379

Regions

Transmembrane93 – 11321Helical; Potential
Domain420 – 45536EF-hand 1
Domain457 – 49236EF-hand 2
Domain493 – 52836EF-hand 3
Calcium binding470 – 481121 Potential
Calcium binding506 – 517122 Potential
Motif178 – 1836HXXXXD motif
Compositional bias27 – 315Poly-Asp

Natural variations

Alternative sequence236 – 28146RKASC…VVVRY → GCKYREKLPAIDFLVCCYSP KEPRLMGKFLFPSNSVLSSL VTLFNL in isoform 2.
VSP_046521
Alternative sequence282 – 539258Missing in isoform 2.
VSP_046522

Experimental info

Sequence conflict2331E → G in BAC42554. Ref.3
Sequence conflict2331E → G in BAD43371. Ref.4
Sequence conflict2331E → G in BAD43419. Ref.4
Sequence conflict2331E → G in BAD43601. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 235A3D368725C5E8

FASTA53961,022
        10         20         30         40         50         60 
MADPDLSSPL IHHQSSDQPE VVISIADDDD DESGLNLLPA VVDPRVSRGF EFDHLNPYGF 

        70         80         90        100        110        120 
LSESEPPVLG PTTVDPFRNN TPGVSGLYEA IKLVICLPIA LIRLVLFAAS LAVGYLATKL 

       130        140        150        160        170        180 
ALAGWKDKEN PMPLWRCRIM WITRICTRCI LFSFGYQWIR RKGKPARREI APIVVSNHVS 

       190        200        210        220        230        240 
YIEPIFYFYE LSPTIVASES HDSLPFVGTI IRAMQVIYVN RFSQTSRKNA VHEIKRKASC 

       250        260        270        280        290        300 
DRFPRLLLFP EGTTTNGKVL ISFQLGAFIP GYPIQPVVVR YPHVHFDQSW GNISLLTLMF 

       310        320        330        340        350        360 
RMFTQFHNFM EVEYLPVIYP SEKQKQNAVR LSQKTSHAIA TSLNVVQTSH SFADLMLLNK 

       370        380        390        400        410        420 
ATELKLENPS NYMVEMARVE SLFHVSSLEA TRFLDTFVSM IPDSSGRVRL HDFLRGLKLK 

       430        440        450        460        470        480 
PCPLSKRIFE FIDVEKVGSI TFKQFLFASG HVLTQPLFKQ TCELAFSHCD ADGDGYITIQ 

       490        500        510        520        530 
ELGEALKNTI PNLNKDEIRG MYHLLDDDQD QRISQNDLLS CLRRNPLLIA IFAPDLAPT 

« Hide

Isoform 2 [UniParc].

Checksum: D469C75B9864D0F5
Show »

FASTA28131,634

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: cv. Columbia.
[5]"UPF3 suppresses aberrant spliced mRNA in Arabidopsis."
Hori K., Watanabe Y.
Plant J. 43:530-540(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING.
[6]"Characterization of two Arabidopsis thaliana acyltransferases with preference for lysophosphatidylethanolamine."
Staalberg K., Staahl U., Stymne S., Ohlrogge J.
BMC Plant Biol. 9:60-60(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC003680 Genomic DNA. Translation: AAC06169.1.
AC003680 Genomic DNA. Translation: AAM14898.1.
CP002685 Genomic DNA. Translation: AEC10584.1.
CP002685 Genomic DNA. Translation: AEC10585.1.
AK117916 mRNA. Translation: BAC42554.1.
AK175608 mRNA. Translation: BAD43371.1.
AK175656 mRNA. Translation: BAD43419.1.
AK175838 mRNA. Translation: BAD43601.1.
AK176251 mRNA. Translation: BAD44014.1.
AK176419 mRNA. Translation: BAD44182.1.
AK176460 mRNA. Translation: BAD44223.1.
PIRT00880.
RefSeqNP_566051.1. NM_130129.4.
NP_566052.1. NM_130130.3.
UniGeneAt.22680.

3D structure databases

ProteinModelPortalQ8S8S2.
SMRQ8S8S2. Positions 384-524.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G45670.1-P.

Proteomic databases

PRIDEQ8S8S2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G45670.1; AT2G45670.1; AT2G45670. [Q8S8S2-1]
GeneID819175.
KEGGath:AT2G45670.

Organism-specific databases

TAIRAT2G45670.

Phylogenomic databases

InParanoidQ8S8S2.
KOK13510.
OMAMVEMARV.
PhylomeDBQ8S8S2.
ProtClustDBCLSN2688948.

Enzyme and pathway databases

BioCycARA:AT2G45670-MONOMER.
UniPathwayUPA00085.

Gene expression databases

GenevestigatorQ8S8S2.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamPF01553. Acyltransferase. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTSM00054. EFh. 3 hits.
SM00563. PlsC. 1 hit.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

PROQ8S8S2.

Entry information

Entry nameLPCT2_ARATH
AccessionPrimary (citable) accession number: Q8S8S2
Secondary accession number(s): O64646, Q8GY20
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2013
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names