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Q8S8S2

- LPCT2_ARATH

UniProt

Q8S8S2 - LPCT2_ARATH

Protein

Lysophospholipid acyltransferase LPEAT2

Gene

LPEAT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Displays acyl-CoA-dependent lysophospholipid acyltransferase activity with a subset of lysophospholipids as substrates. Exhibits strong acylation activity of lysophosphatidylethanolamine (LPE) and lower activity on lysophosphatidate (LPA). Has a preference for 18:1-LPE over 16:0-LPE as acceptor and palmitoyl-CoA (16:0-CoA) as acyl donor. Activity is calcium-independent.1 Publication

    Catalytic activityi

    Acyl-CoA + 1-O-(alk-1-enyl)glycero-3-phosphocholine = CoA + plasmenylcholine.1 Publication

    pH dependencei

    Optimum pH is 9.0.1 Publication

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi470 – 481121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi506 – 517122PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. plasmalogen synthase activity Source: UniProtKB

    GO - Biological processi

    1. phospholipid biosynthetic process Source: UniProtKB-KW
    2. phospholipid metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    BioCyciARA:AT2G45670-MONOMER.
    ReactomeiREACT_185985. Synthesis of PA.
    REACT_187670. Acyl chain remodelling of PG.
    REACT_187673. Acyl chain remodelling of PC.
    REACT_187682. Acyl chain remodelling of PE.
    REACT_187684. Acyl chain remodelling of PS.
    UniPathwayiUPA00085.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysophospholipid acyltransferase LPEAT2 (EC:2.3.1.25)
    Alternative name(s):
    Lysophosphatidylethanolamine acyltransferase 2
    Short name:
    AtLPEAT2
    Gene namesi
    Name:LPEAT2
    Ordered Locus Names:At2g45670
    ORF Names:F17K2.20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G45670.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 539539Lysophospholipid acyltransferase LPEAT2PRO_0000422379Add
    BLAST

    Proteomic databases

    PRIDEiQ8S8S2.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8S8S2.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G45670.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8S8S2.
    SMRiQ8S8S2. Positions 384-524.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei93 – 11321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini420 – 45536EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini457 – 49236EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini493 – 52836EF-hand 3PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi178 – 1836HXXXXD motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi27 – 315Poly-Asp

    Domaini

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphocholine.By similarity

    Sequence similaritiesi

    Contains 3 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    InParanoidiQ8S8S2.
    KOiK13510.
    OMAiIVASESH.
    PhylomeDBiQ8S8S2.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002123. Plipid/glycerol_acylTrfase.
    [Graphical view]
    PfamiPF01553. Acyltransferase. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 3 hits.
    SM00563. PlsC. 1 hit.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 3 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8S8S2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MADPDLSSPL IHHQSSDQPE VVISIADDDD DESGLNLLPA VVDPRVSRGF    50
    EFDHLNPYGF LSESEPPVLG PTTVDPFRNN TPGVSGLYEA IKLVICLPIA 100
    LIRLVLFAAS LAVGYLATKL ALAGWKDKEN PMPLWRCRIM WITRICTRCI 150
    LFSFGYQWIR RKGKPARREI APIVVSNHVS YIEPIFYFYE LSPTIVASES 200
    HDSLPFVGTI IRAMQVIYVN RFSQTSRKNA VHEIKRKASC DRFPRLLLFP 250
    EGTTTNGKVL ISFQLGAFIP GYPIQPVVVR YPHVHFDQSW GNISLLTLMF 300
    RMFTQFHNFM EVEYLPVIYP SEKQKQNAVR LSQKTSHAIA TSLNVVQTSH 350
    SFADLMLLNK ATELKLENPS NYMVEMARVE SLFHVSSLEA TRFLDTFVSM 400
    IPDSSGRVRL HDFLRGLKLK PCPLSKRIFE FIDVEKVGSI TFKQFLFASG 450
    HVLTQPLFKQ TCELAFSHCD ADGDGYITIQ ELGEALKNTI PNLNKDEIRG 500
    MYHLLDDDQD QRISQNDLLS CLRRNPLLIA IFAPDLAPT 539
    Length:539
    Mass (Da):61,022
    Last modified:June 1, 2002 - v1
    Checksum:i235A3D368725C5E8
    GO
    Isoform 2 (identifier: Q8S8S2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         236-281: RKASCDRFPR...YPIQPVVVRY → GCKYREKLPA...LSSLVTLFNL
         282-539: Missing.

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

    Show »
    Length:281
    Mass (Da):31,634
    Checksum:iD469C75B9864D0F5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti233 – 2331E → G in BAC42554. (PubMed:11910074)Curated
    Sequence conflicti233 – 2331E → G in BAD43371. 1 PublicationCurated
    Sequence conflicti233 – 2331E → G in BAD43419. 1 PublicationCurated
    Sequence conflicti233 – 2331E → G in BAD43601. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei236 – 28146RKASC…VVVRY → GCKYREKLPAIDFLVCCYSP KEPRLMGKFLFPSNSVLSSL VTLFNL in isoform 2. 1 PublicationVSP_046521Add
    BLAST
    Alternative sequencei282 – 539258Missing in isoform 2. 1 PublicationVSP_046522Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC003680 Genomic DNA. Translation: AAC06169.1.
    AC003680 Genomic DNA. Translation: AAM14898.1.
    CP002685 Genomic DNA. Translation: AEC10584.1.
    CP002685 Genomic DNA. Translation: AEC10585.1.
    AK117916 mRNA. Translation: BAC42554.1.
    AK175608 mRNA. Translation: BAD43371.1.
    AK175656 mRNA. Translation: BAD43419.1.
    AK175838 mRNA. Translation: BAD43601.1.
    AK176251 mRNA. Translation: BAD44014.1.
    AK176419 mRNA. Translation: BAD44182.1.
    AK176460 mRNA. Translation: BAD44223.1.
    PIRiT00880.
    RefSeqiNP_566051.1. NM_130129.4. [Q8S8S2-1]
    NP_566052.1. NM_130130.3. [Q8S8S2-2]
    UniGeneiAt.22680.

    Genome annotation databases

    EnsemblPlantsiAT2G45670.1; AT2G45670.1; AT2G45670. [Q8S8S2-1]
    GeneIDi819175.
    KEGGiath:AT2G45670.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC003680 Genomic DNA. Translation: AAC06169.1 .
    AC003680 Genomic DNA. Translation: AAM14898.1 .
    CP002685 Genomic DNA. Translation: AEC10584.1 .
    CP002685 Genomic DNA. Translation: AEC10585.1 .
    AK117916 mRNA. Translation: BAC42554.1 .
    AK175608 mRNA. Translation: BAD43371.1 .
    AK175656 mRNA. Translation: BAD43419.1 .
    AK175838 mRNA. Translation: BAD43601.1 .
    AK176251 mRNA. Translation: BAD44014.1 .
    AK176419 mRNA. Translation: BAD44182.1 .
    AK176460 mRNA. Translation: BAD44223.1 .
    PIRi T00880.
    RefSeqi NP_566051.1. NM_130129.4. [Q8S8S2-1 ]
    NP_566052.1. NM_130130.3. [Q8S8S2-2 ]
    UniGenei At.22680.

    3D structure databases

    ProteinModelPortali Q8S8S2.
    SMRi Q8S8S2. Positions 384-524.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G45670.1-P.

    Proteomic databases

    PRIDEi Q8S8S2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G45670.1 ; AT2G45670.1 ; AT2G45670 . [Q8S8S2-1 ]
    GeneIDi 819175.
    KEGGi ath:AT2G45670.

    Organism-specific databases

    TAIRi AT2G45670.

    Phylogenomic databases

    InParanoidi Q8S8S2.
    KOi K13510.
    OMAi IVASESH.
    PhylomeDBi Q8S8S2.

    Enzyme and pathway databases

    UniPathwayi UPA00085 .
    BioCyci ARA:AT2G45670-MONOMER.
    Reactomei REACT_185985. Synthesis of PA.
    REACT_187670. Acyl chain remodelling of PG.
    REACT_187673. Acyl chain remodelling of PC.
    REACT_187682. Acyl chain remodelling of PE.
    REACT_187684. Acyl chain remodelling of PS.

    Miscellaneous databases

    PROi Q8S8S2.

    Gene expression databases

    Genevestigatori Q8S8S2.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002123. Plipid/glycerol_acylTrfase.
    [Graphical view ]
    Pfami PF01553. Acyltransferase. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 3 hits.
    SM00563. PlsC. 1 hit.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: cv. Columbia.
    5. "UPF3 suppresses aberrant spliced mRNA in Arabidopsis."
      Hori K., Watanabe Y.
      Plant J. 43:530-540(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING.
    6. "Characterization of two Arabidopsis thaliana acyltransferases with preference for lysophosphatidylethanolamine."
      Staalberg K., Staahl U., Stymne S., Ohlrogge J.
      BMC Plant Biol. 9:60-60(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.

    Entry informationi

    Entry nameiLPCT2_ARATH
    AccessioniPrimary (citable) accession number: Q8S8S2
    Secondary accession number(s): O64646, Q8GY20
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2013
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The ratio of isoform 2/isoform 1 mRNA is increased about 15-fold in the nonsense mRNA reducing factor mutant upf3-1.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3