Q8S8S2 (LPCT2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysophospholipid acyltransferase LPEAT2
EC=2.3.1.25

Alternative name(s):
Lysophosphatidylethanolamine acyltransferase 2
Short name=AtLPEAT2

Gene names

Name:	LPEAT2
Ordered Locus Names:	At2g45670
ORF Names:	F17K2.20

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Displays acyl-CoA-dependent lysophospholipid acyltransferase activity with a subset of lysophospholipids as substrates. Exhibits strong acylation activity of lysophosphatidylethanolamine (LPE) and lower activity on lysophosphatidate (LPA). Has a preference for 18:1-LPE over 16:0-LPE as acceptor and palmitoyl-CoA (16:0-CoA) as acyl donor. Activity is calcium-independent. Ref.6
Catalytic activity	Acyl-CoA + 1-O-(alk-1-enyl)glycero-3-phosphocholine = CoA + plasmenylcholine. Ref.6
Pathway	Lipid metabolism; phospholipid metabolism.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphocholine By similarity.
Miscellaneous	The ratio of isoform 2/isoform 1 mRNA is increased about 15-fold in the nonsense mRNA reducing factor mutant upf3-1 (Ref.5).
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. Contains 3 EF-hand domains.
Biophysicochemical properties	pH dependence: Optimum pH is 9.0. Ref.6

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Transmembrane Transmembrane helix
Ligand	Calcium
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring acyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8S8S2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8S8S2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     236-281: RKASCDRFPR...YPIQPVVVRY → GCKYREKLPA...LSSLVTLFNL
     282-539: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 539	539	Lysophospholipid acyltransferase LPEAT2		PRO_0000422379
Regions
	Transmembrane	93 – 113	21	Helical; Potential
	Domain	420 – 455	36	EF-hand 1
	Domain	457 – 492	36	EF-hand 2
	Domain	493 – 528	36	EF-hand 3
	Calcium binding	470 – 481	12	1 Potential
	Calcium binding	506 – 517	12	2 Potential
	Motif	178 – 183	6	HXXXXD motif
	Compositional bias	27 – 31	5	Poly-Asp
Natural variations
	Alternative sequence	236 – 281	46	RKASC…VVVRY → GCKYREKLPAIDFLVCCYSP KEPRLMGKFLFPSNSVLSSL VTLFNL in isoform 2.		VSP_046521
	Alternative sequence	282 – 539	258	Missing in isoform 2.		VSP_046522
Experimental info
	Sequence conflict	233	1	E → G in BAC42554. Ref.3
	Sequence conflict	233	1	E → G in BAD43371. Ref.4
	Sequence conflict	233	1	E → G in BAD43419. Ref.4
	Sequence conflict	233	1	E → G in BAD43601. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 235A3D368725C5E8	FASTA	539	61,022
	10 20 30 40 50 60 MADPDLSSPL IHHQSSDQPE VVISIADDDD DESGLNLLPA VVDPRVSRGF EFDHLNPYGF 70 80 90 100 110 120 LSESEPPVLG PTTVDPFRNN TPGVSGLYEA IKLVICLPIA LIRLVLFAAS LAVGYLATKL 130 140 150 160 170 180 ALAGWKDKEN PMPLWRCRIM WITRICTRCI LFSFGYQWIR RKGKPARREI APIVVSNHVS 190 200 210 220 230 240 YIEPIFYFYE LSPTIVASES HDSLPFVGTI IRAMQVIYVN RFSQTSRKNA VHEIKRKASC 250 260 270 280 290 300 DRFPRLLLFP EGTTTNGKVL ISFQLGAFIP GYPIQPVVVR YPHVHFDQSW GNISLLTLMF 310 320 330 340 350 360 RMFTQFHNFM EVEYLPVIYP SEKQKQNAVR LSQKTSHAIA TSLNVVQTSH SFADLMLLNK 370 380 390 400 410 420 ATELKLENPS NYMVEMARVE SLFHVSSLEA TRFLDTFVSM IPDSSGRVRL HDFLRGLKLK 430 440 450 460 470 480 PCPLSKRIFE FIDVEKVGSI TFKQFLFASG HVLTQPLFKQ TCELAFSHCD ADGDGYITIQ 490 500 510 520 530 ELGEALKNTI PNLNKDEIRG MYHLLDDDQD QRISQNDLLS CLRRNPLLIA IFAPDLAPT
	Isoform 2 [UniParc]. Checksum: D469C75B9864D0F5	FASTA	281	31,634
	10 20 30 40 50 60 MADPDLSSPL IHHQSSDQPE VVISIADDDD DESGLNLLPA VVDPRVSRGF EFDHLNPYGF 70 80 90 100 110 120 LSESEPPVLG PTTVDPFRNN TPGVSGLYEA IKLVICLPIA LIRLVLFAAS LAVGYLATKL 130 140 150 160 170 180 ALAGWKDKEN PMPLWRCRIM WITRICTRCI LFSFGYQWIR RKGKPARREI APIVVSNHVS 190 200 210 220 230 240 YIEPIFYFYE LSPTIVASES HDSLPFVGTI IRAMQVIYVN RFSQTSRKNA VHEIKGCKYR 250 260 270 280 EKLPAIDFLV CCYSPKEPRL MGKFLFPSNS VLSSLVTLFN L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: cv. Columbia.
[5]	"UPF3 suppresses aberrant spliced mRNA in Arabidopsis." Hori K., Watanabe Y. Plant J. 43:530-540(2005) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING.
[6]	"Characterization of two Arabidopsis thaliana acyltransferases with preference for lysophosphatidylethanolamine." Staalberg K., Staahl U., Stymne S., Ohlrogge J. BMC Plant Biol. 9:60-60(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC003680 Genomic DNA. Translation: AAC06169.1. AC003680 Genomic DNA. Translation: AAM14898.1. CP002685 Genomic DNA. Translation: AEC10584.1. CP002685 Genomic DNA. Translation: AEC10585.1. AK117916 mRNA. Translation: BAC42554.1. AK175608 mRNA. Translation: BAD43371.1. AK175656 mRNA. Translation: BAD43419.1. AK175838 mRNA. Translation: BAD43601.1. AK176251 mRNA. Translation: BAD44014.1. AK176419 mRNA. Translation: BAD44182.1. AK176460 mRNA. Translation: BAD44223.1.
IPI	IPI00537175.
PIR	T00880.
RefSeq	NP_566051.1. NM_130129.4.
UniGene	At.22680.
3D structure databases
SMR	Q8S8S2. Positions 383-529.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G45670.1; AT2G45670.1; AT2G45670.
GeneID	819175.
KEGG	ath:AT2G45670.
Organism-specific databases
TAIR	At2g45670.
Phylogenomic databases
InParanoid	Q8S8S2.
KO	K13510.
OMA	MVEMARV.
PhylomeDB	Q8S8S2.
ProtClustDB	CLSN2688948.
Enzyme and pathway databases
UniPathway	UPA00085.
Gene expression databases
Genevestigator	Q8S8S2.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR002123. Plipid/glycerol_acylTrfase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. PF13499. EF_hand_5. 1 hit. [Graphical view]
SMART	SM00054. EFh. 3 hits. SM00563. PlsC. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPCT2_ARATH

Accession

Primary (citable) accession number: Q8S8S2
Secondary accession number(s): O64646, Q8GY20

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2013
Last sequence update:	June 1, 2002
Last modified:	May 29, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program