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Q8S8S2

- LPCT2_ARATH

UniProt

Q8S8S2 - LPCT2_ARATH

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Protein

Lysophospholipid acyltransferase LPEAT2

Gene

LPEAT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Displays acyl-CoA-dependent lysophospholipid acyltransferase activity with a subset of lysophospholipids as substrates. Exhibits strong acylation activity of lysophosphatidylethanolamine (LPE) and lower activity on lysophosphatidate (LPA). Has a preference for 18:1-LPE over 16:0-LPE as acceptor and palmitoyl-CoA (16:0-CoA) as acyl donor. Activity is calcium-independent.1 Publication

Catalytic activityi

Acyl-CoA + 1-O-(alk-1-enyl)glycero-3-phosphocholine = CoA + plasmenylcholine.1 Publication

pH dependencei

Optimum pH is 9.0.1 Publication

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi470 – 481121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi506 – 517122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. plasmalogen synthase activity Source: UniProtKB

GO - Biological processi

  1. phospholipid biosynthetic process Source: UniProtKB-KW
  2. phospholipid metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BioCyciARA:AT2G45670-MONOMER.
ReactomeiREACT_185985. Synthesis of PA.
REACT_187670. Acyl chain remodelling of PG.
REACT_187673. Acyl chain remodelling of PC.
REACT_187682. Acyl chain remodelling of PE.
REACT_187684. Acyl chain remodelling of PS.
UniPathwayiUPA00085.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipid acyltransferase LPEAT2 (EC:2.3.1.25)
Alternative name(s):
Lysophosphatidylethanolamine acyltransferase 2
Short name:
AtLPEAT2
Gene namesi
Name:LPEAT2
Ordered Locus Names:At2g45670
ORF Names:F17K2.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G45670.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 539539Lysophospholipid acyltransferase LPEAT2PRO_0000422379Add
BLAST

Proteomic databases

PRIDEiQ8S8S2.

Expressioni

Gene expression databases

ExpressionAtlasiQ8S8S2. baseline and differential.
GenevestigatoriQ8S8S2.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G45670.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8S8S2.
SMRiQ8S8S2. Positions 384-524.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei93 – 11321HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini420 – 45536EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini457 – 49236EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini493 – 52836EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi178 – 1836HXXXXD motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi27 – 315Poly-Asp

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphocholine.By similarity

Sequence similaritiesi

Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiQ8S8S2.
KOiK13510.
OMAiIVASESH.
PhylomeDBiQ8S8S2.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
SM00563. PlsC. 1 hit.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8S8S2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADPDLSSPL IHHQSSDQPE VVISIADDDD DESGLNLLPA VVDPRVSRGF
60 70 80 90 100
EFDHLNPYGF LSESEPPVLG PTTVDPFRNN TPGVSGLYEA IKLVICLPIA
110 120 130 140 150
LIRLVLFAAS LAVGYLATKL ALAGWKDKEN PMPLWRCRIM WITRICTRCI
160 170 180 190 200
LFSFGYQWIR RKGKPARREI APIVVSNHVS YIEPIFYFYE LSPTIVASES
210 220 230 240 250
HDSLPFVGTI IRAMQVIYVN RFSQTSRKNA VHEIKRKASC DRFPRLLLFP
260 270 280 290 300
EGTTTNGKVL ISFQLGAFIP GYPIQPVVVR YPHVHFDQSW GNISLLTLMF
310 320 330 340 350
RMFTQFHNFM EVEYLPVIYP SEKQKQNAVR LSQKTSHAIA TSLNVVQTSH
360 370 380 390 400
SFADLMLLNK ATELKLENPS NYMVEMARVE SLFHVSSLEA TRFLDTFVSM
410 420 430 440 450
IPDSSGRVRL HDFLRGLKLK PCPLSKRIFE FIDVEKVGSI TFKQFLFASG
460 470 480 490 500
HVLTQPLFKQ TCELAFSHCD ADGDGYITIQ ELGEALKNTI PNLNKDEIRG
510 520 530
MYHLLDDDQD QRISQNDLLS CLRRNPLLIA IFAPDLAPT
Length:539
Mass (Da):61,022
Last modified:June 1, 2002 - v1
Checksum:i235A3D368725C5E8
GO
Isoform 2 (identifier: Q8S8S2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     236-281: RKASCDRFPR...YPIQPVVVRY → GCKYREKLPA...LSSLVTLFNL
     282-539: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Show »
Length:281
Mass (Da):31,634
Checksum:iD469C75B9864D0F5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti233 – 2331E → G in BAC42554. (PubMed:11910074)Curated
Sequence conflicti233 – 2331E → G in BAD43371. 1 PublicationCurated
Sequence conflicti233 – 2331E → G in BAD43419. 1 PublicationCurated
Sequence conflicti233 – 2331E → G in BAD43601. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei236 – 28146RKASC…VVVRY → GCKYREKLPAIDFLVCCYSP KEPRLMGKFLFPSNSVLSSL VTLFNL in isoform 2. 1 PublicationVSP_046521Add
BLAST
Alternative sequencei282 – 539258Missing in isoform 2. 1 PublicationVSP_046522Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC003680 Genomic DNA. Translation: AAC06169.1.
AC003680 Genomic DNA. Translation: AAM14898.1.
CP002685 Genomic DNA. Translation: AEC10584.1.
CP002685 Genomic DNA. Translation: AEC10585.1.
AK117916 mRNA. Translation: BAC42554.1.
AK175608 mRNA. Translation: BAD43371.1.
AK175656 mRNA. Translation: BAD43419.1.
AK175838 mRNA. Translation: BAD43601.1.
AK176251 mRNA. Translation: BAD44014.1.
AK176419 mRNA. Translation: BAD44182.1.
AK176460 mRNA. Translation: BAD44223.1.
PIRiT00880.
RefSeqiNP_566051.1. NM_130129.4. [Q8S8S2-1]
NP_566052.1. NM_130130.3. [Q8S8S2-2]
UniGeneiAt.22680.

Genome annotation databases

EnsemblPlantsiAT2G45670.1; AT2G45670.1; AT2G45670. [Q8S8S2-1]
GeneIDi819175.
KEGGiath:AT2G45670.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC003680 Genomic DNA. Translation: AAC06169.1 .
AC003680 Genomic DNA. Translation: AAM14898.1 .
CP002685 Genomic DNA. Translation: AEC10584.1 .
CP002685 Genomic DNA. Translation: AEC10585.1 .
AK117916 mRNA. Translation: BAC42554.1 .
AK175608 mRNA. Translation: BAD43371.1 .
AK175656 mRNA. Translation: BAD43419.1 .
AK175838 mRNA. Translation: BAD43601.1 .
AK176251 mRNA. Translation: BAD44014.1 .
AK176419 mRNA. Translation: BAD44182.1 .
AK176460 mRNA. Translation: BAD44223.1 .
PIRi T00880.
RefSeqi NP_566051.1. NM_130129.4. [Q8S8S2-1 ]
NP_566052.1. NM_130130.3. [Q8S8S2-2 ]
UniGenei At.22680.

3D structure databases

ProteinModelPortali Q8S8S2.
SMRi Q8S8S2. Positions 384-524.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G45670.1-P.

Proteomic databases

PRIDEi Q8S8S2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G45670.1 ; AT2G45670.1 ; AT2G45670 . [Q8S8S2-1 ]
GeneIDi 819175.
KEGGi ath:AT2G45670.

Organism-specific databases

TAIRi AT2G45670.

Phylogenomic databases

InParanoidi Q8S8S2.
KOi K13510.
OMAi IVASESH.
PhylomeDBi Q8S8S2.

Enzyme and pathway databases

UniPathwayi UPA00085 .
BioCyci ARA:AT2G45670-MONOMER.
Reactomei REACT_185985. Synthesis of PA.
REACT_187670. Acyl chain remodelling of PG.
REACT_187673. Acyl chain remodelling of PC.
REACT_187682. Acyl chain remodelling of PE.
REACT_187684. Acyl chain remodelling of PS.

Miscellaneous databases

PROi Q8S8S2.

Gene expression databases

ExpressionAtlasi Q8S8S2. baseline and differential.
Genevestigatori Q8S8S2.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view ]
Pfami PF01553. Acyltransferase. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view ]
SMARTi SM00054. EFh. 3 hits.
SM00563. PlsC. 1 hit.
[Graphical view ]
PROSITEi PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: cv. Columbia.
  5. "UPF3 suppresses aberrant spliced mRNA in Arabidopsis."
    Hori K., Watanabe Y.
    Plant J. 43:530-540(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING.
  6. "Characterization of two Arabidopsis thaliana acyltransferases with preference for lysophosphatidylethanolamine."
    Staalberg K., Staahl U., Stymne S., Ohlrogge J.
    BMC Plant Biol. 9:60-60(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.

Entry informationi

Entry nameiLPCT2_ARATH
AccessioniPrimary (citable) accession number: Q8S8S2
Secondary accession number(s): O64646, Q8GY20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2013
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The ratio of isoform 2/isoform 1 mRNA is increased about 15-fold in the nonsense mRNA reducing factor mutant upf3-1.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3