ID   GUN14_ARATH             Reviewed;         491 AA.
AC   Q8S8Q4; O64891;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 2.
DT   27-MAR-2024, entry version 123.
DE   RecName: Full=Endoglucanase 14;
DE            EC=3.2.1.4;
DE   AltName: Full=Endo-1,4-beta glucanase 14;
DE   Flags: Precursor;
GN   OrderedLocusNames=At2g44560; ORFNames=F16B22.5, F4I1.39;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   GENE FAMILY.
RX   PubMed=15170254; DOI=10.1007/s00239-003-2571-x;
RA   Libertini E., Li Y., McQueen-Mason S.J.;
RT   "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family.";
RL   J. Mol. Evol. 58:506-515(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM14961.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC003672; AAC27458.1; -; Genomic_DNA.
DR   EMBL; AC004521; AAM14961.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002685; AEC10438.1; -; Genomic_DNA.
DR   EMBL; AK175889; BAD43652.1; -; mRNA.
DR   PIR; T01583; T01583.
DR   RefSeq; NP_181984.1; NM_130020.3.
DR   AlphaFoldDB; Q8S8Q4; -.
DR   SMR; Q8S8Q4; -.
DR   STRING; 3702.Q8S8Q4; -.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   PaxDb; 3702-AT2G44560-1; -.
DR   ProteomicsDB; 247266; -.
DR   EnsemblPlants; AT2G44560.1; AT2G44560.1; AT2G44560.
DR   GeneID; 819064; -.
DR   Gramene; AT2G44560.1; AT2G44560.1; AT2G44560.
DR   KEGG; ath:AT2G44560; -.
DR   Araport; AT2G44560; -.
DR   TAIR; AT2G44560; GH9B11.
DR   eggNOG; ENOG502QRF6; Eukaryota.
DR   HOGENOM; CLU_008926_1_4_1; -.
DR   InParanoid; Q8S8Q4; -.
DR   OMA; EEYICSC; -.
DR   OrthoDB; 1347382at2759; -.
DR   PhylomeDB; Q8S8Q4; -.
DR   BioCyc; ARA:AT2G44560-MONOMER; -.
DR   PRO; PR:Q8S8Q4; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q8S8Q4; baseline and differential.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR   PANTHER; PTHR22298:SF125; ENDOGLUCANASE 12-RELATED; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   Genevisible; Q8S8Q4; AT.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW   Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..31
FT                   /evidence="ECO:0000255"
FT   CHAIN           32..491
FT                   /note="Endoglucanase 14"
FT                   /id="PRO_0000249266"
FT   ACT_SITE        86
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT   ACT_SITE        413
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        465
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        474
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   CARBOHYD        397
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   491 AA;  53198 MW;  7F9250C761AA8484 CRC64;
     MSQLKIGSSQ CLWTSICIVL FVLSMARGAV SRNYGDALTK SLLYFEAQRS GKLPSNQRVT
     WRGDSALRDG SDAHVDLTGG YYDAGDNMKF GFPLAFFTTM LAWSNIEMAT QLKAHQEQEN
     ALAALKWATD FLIKAHPEPN VLYGQVGDGN SDHECWMRPE DMTTPRPSFR IDAQHPGSDL
     AGETAAAMAA ASIAFAPSDE AYAQILIGHA KELFEFAKAY PGIYQNSITN AGGFYASSGY
     EDELLWAAAW LHRATNDQIY LDYLTQASGT GGPRTAFSWD DKFVGAQVLV AKLALEGKVE
     SNGKIAEYKS MAEQFICNCA QKGSNNVKKT PGGLLYFLPW NNLQYTTAAT FVLSAYSKYL
     TDAKASIQCP NGALQASDLL DLARSQVDYI LGSNPQNMSY MVGVGTNYPK KPHHRAASIV
     SITKDKTPVT CSEGFDAWFN NPAPNPNVLM GAVVGGPNDN DVYGDERTDY QHAEPAPATA
     APFVGVLAAV A
//