Q8S8Q4 (GUN14_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 14
EC=3.2.1.4

Alternative name(s):
Endo-1,4-beta glucanase 14

Gene names

Ordered Locus Names:	At2g44560
ORF Names:	F16B22.5, F4I1.39

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.
Sequence caution	The sequence AAM14961.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 491	460	Endoglucanase 14		PRO_0000249266
Sites
	Active site	413	1	By similarity
	Active site	465	1	By similarity
	Active site	474	1	By similarity
Amino acid modifications
	Glycosylation	397	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8S8Q4 [UniParc]. Last modified September 5, 2006. Version 2. Checksum: 7F9250C761AA8484	FASTA	491	53,198
	10 20 30 40 50 60 MSQLKIGSSQ CLWTSICIVL FVLSMARGAV SRNYGDALTK SLLYFEAQRS GKLPSNQRVT 70 80 90 100 110 120 WRGDSALRDG SDAHVDLTGG YYDAGDNMKF GFPLAFFTTM LAWSNIEMAT QLKAHQEQEN 130 140 150 160 170 180 ALAALKWATD FLIKAHPEPN VLYGQVGDGN SDHECWMRPE DMTTPRPSFR IDAQHPGSDL 190 200 210 220 230 240 AGETAAAMAA ASIAFAPSDE AYAQILIGHA KELFEFAKAY PGIYQNSITN AGGFYASSGY 250 260 270 280 290 300 EDELLWAAAW LHRATNDQIY LDYLTQASGT GGPRTAFSWD DKFVGAQVLV AKLALEGKVE 310 320 330 340 350 360 SNGKIAEYKS MAEQFICNCA QKGSNNVKKT PGGLLYFLPW NNLQYTTAAT FVLSAYSKYL 370 380 390 400 410 420 TDAKASIQCP NGALQASDLL DLARSQVDYI LGSNPQNMSY MVGVGTNYPK KPHHRAASIV 430 440 450 460 470 480 SITKDKTPVT CSEGFDAWFN NPAPNPNVLM GAVVGGPNDN DVYGDERTDY QHAEPAPATA 490 APFVGVLAAV A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family." Libertini E., Li Y., McQueen-Mason S.J. J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC003672 Genomic DNA. Translation: AAC27458.1. AC004521 Genomic DNA. Translation: AAM14961.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC10438.1. AK175889 mRNA. Translation: BAD43652.1.
IPI	IPI00534045.
PIR	T01583.
RefSeq	NP_181984.1. NM_130020.2.
UniGene	At.36743.
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ProteinModelPortal	Q8S8Q4.
SMR	Q8S8Q4. Positions 33-488.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Proteomic databases
PaxDb	Q8S8Q4.
PRIDE	Q8S8Q4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G44560.1; AT2G44560.1; AT2G44560.
GeneID	819064.
KEGG	ath:AT2G44560.
Organism-specific databases
TAIR	At2g44560.
Phylogenomic databases
eggNOG	NOG05134.
HOGENOM	HOG000021033.
InParanoid	Q8S8Q4.
OMA	DRYDDSR.
PhylomeDB	Q8S8Q4.
ProtClustDB	PLN00119.
Gene expression databases
Genevestigator	Q8S8Q4.
GermOnline	AT2G44560. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN14_ARATH

Accession

Primary (citable) accession number: Q8S8Q4
Secondary accession number(s): O64891

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	September 5, 2006
Last modified:	May 1, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program