Reviewed, UniProtKB/Swiss-Prot Q8S895 (SAT2_ARATH)

Names and origin

Protein names

Recommended name:
    Serine acetyltransferase 2
      Short name=AtSAT-2
      Short name=AtSERAT3;1
    EC=2.3.1.30

Gene names

Name:	SAT2
Synonyms:	SAT106
Ordered Locus Names:	At2g17640
ORF Names:	T17A5.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2.
Subunit structure	Homomultimer By similarity.
Subcellular location	Cytoplasm. Ref.6
Tissue specificity	Ubiquitously expressed at low levels. Localized in vascular tissues, particularly in phloem. Ref.6 Ref.1
Induction	By cadmium (Cd). Induced in roots under sulfur-deficient conditions. Ref.6 Ref.1
Sequence similarities	Belongs to the transferase hexapeptide repeat family.
Biophysicochemical properties	Kinetic parameters: KM=121.4 mM for L-Ser (at pH 8 and 30 degrees Celsius) KM=24.5 mM for acetyl-CoA (at pH 8 and 30 degrees Celsius)
Sequence caution	The sequence AAD45614.2 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM15485.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro sulfate assimilation Ref.6 Traceable author statement. Source: TAIR
Cellular component	cytosol Ref.6 Inferred from direct assay. Source: TAIR
Molecular function	serine O-acetyltransferase activity Ref.6 Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Serine acetyltransferase 2		PRO_0000068691
Regions
	Compositional bias	178 – 244	67	Gly-rich

Sequences

	Sequence		Length	Mass (Da)
	Q8S895-1 [UniParc]. Last modified January 24, 2006. Version 2. Checksum: 8CE538962B44E610	FASTA	323	34,534
	10 20 30 40 50 60 MNGDELPFES GFEVYAKGTH KSEFDSNLLD PRSDPIWDAI REEAKLEAEK EPILSSFLYA 70 80 90 100 110 120 GILAHDCLEQ ALGFVLANRL QNPTLLATQL LDIFYGVMMH DKGIQSSIRH DLQAFKDRDP 130 140 150 160 170 180 ACLSYSSAIL HLKGYHALQA YRVAHKLWNE GRKLLALALQ SRISEVFGID IHPAARIGEG 190 200 210 220 230 240 ILLDHGTGVV IGETAVIGNG VSILHGVTLG GTGKETGDRH PKIGEGALLG ACVTILGNIS 250 260 270 280 290 300 IGAGAMVAAG SLVLKDVPSH SVVAGNPAKL IRVMEEQDPS LAMKHDATKE FFRHVADGYK 310 320 GAQSNGPSLS AGDTEKGHTN STS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The serine acetyltransferase gene family in Arabidopsis thaliana and the regulation of its expression by cadmium." Howarth J.R., Dominguez-Solis J.R., Gutierrez-Alcala G., Wray J.L., Romero L.C., Gotor C. Plant Mol. Biol. 51:589-598(2003) [PubMed: 12650624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Gene sequence of serine acetyltranferase 2 from A. thaliana." Ruffet M.-L., Lebrun M., Droux M., Douce R. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Arabidopsis ORF clones." Shinn P., Chen H., Kim C.J., Ecker J.R. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Characterization and expression analysis of a serine acetyltransferase gene family involved in a key step of the sulfur assimilation pathway in Arabidopsis." Kawashima C.G., Berkowitz O., Hell R., Noji M., Saito K. Plant Physiol. 137:220-230(2005) [PubMed: 15579666] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF112303 mRNA. Translation: AAD19963.1. L78444 Genomic DNA. Translation: AAD45614.2. Sequence problems. AC007509 Genomic DNA. Translation: AAM15485.1. Sequence problems. AK176573 mRNA. Translation: BAD44336.1. BT024717 mRNA. Translation: ABD59055.1.
IPI	IPI00518737.
PIR	T08867.
RefSeq	NP_565421.1.
UniGene	At.11051
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q8S895.
Genome annotation databases
GeneID	816271.
GenomeReviews	Gene locus AT2G17640 in contig CT485783_GR.
KEGG	ath:AT2G17640.
NMPDR	fig|3702.1.peg.8754.
Organism-specific databases
GeneFarm	5178. 495.
TAIR	At2g17640.
Phylogenomic databases
OMA	FHALESH.
Enzyme and pathway databases
BRENDA	2.3.1.30. 302.
Gene expression databases
Genevestigator	Q8S895.
GermOnline	AT2G17640. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR010493. Ser_AcTrfase_N. IPR005881. Ser_O-AcTrfase. [Graphical view]
Pfam	PF00132. Hexapep. 3 hits. PF06426. SATase_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01172. cysE. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAT2_ARATH

Accession

Primary (citable) accession number: Q8S895
Secondary accession number(s): Q29Q39, Q9STB0, Q9ZPJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	January 24, 2006
Last modified:	November 3, 2009
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)