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Protein

Acetyl-CoA acetyltransferase, cytosolic 1

Gene

AAT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2 acetyl-CoA = CoA + acetoacetyl-CoA.PROSITE-ProRule annotation

Pathwayi: (R)-mevalonate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-mevalonate from acetyl-CoA.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Acetyl-CoA acetyltransferase, cytosolic 1 (AAT1)
  2. Hydroxymethylglutaryl-CoA synthase (HMGS)
  3. 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 (HMG1), 3-hydroxy-3-methylglutaryl-coenzyme A reductase 2 (HMG2)
This subpathway is part of the pathway (R)-mevalonate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-mevalonate from acetyl-CoA, the pathway (R)-mevalonate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei97 – 971Acyl-thioester intermediateBy similarity
Binding sitei237 – 2371Coenzyme ABy similarity
Metal bindingi254 – 2541Potassium; via carbonyl oxygenBy similarity
Metal bindingi255 – 2551Potassium; via carbonyl oxygenBy similarity
Metal bindingi257 – 2571Potassium; via carbonyl oxygenBy similarity
Binding sitei258 – 2581Coenzyme ABy similarity
Metal bindingi355 – 3551Potassium; via carbonyl oxygenBy similarity
Active sitei359 – 3591Proton acceptorPROSITE-ProRule annotation
Active sitei389 – 3891Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  • acetyl-CoA C-acetyltransferase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • isoprenoid biosynthetic process Source: UniProtKB-KW
  • pollen germination Source: TAIR
  • pollen tube growth Source: TAIR
  • sterol metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Metal-binding, Potassium

Enzyme and pathway databases

BioCyciARA:GQT-183-MONOMER.
BRENDAi2.3.1.9. 399.
ReactomeiR-ATH-70895. Branched-chain amino acid catabolism.
R-ATH-77108. Utilization of Ketone Bodies.
R-ATH-77111. Synthesis of Ketone Bodies.
UniPathwayiUPA00058; UER00101.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-CoA acetyltransferase, cytosolic 1 (EC:2.3.1.9)
Alternative name(s):
Cytosolic acetoacetyl-CoA thiolase 1
Short name:
Thiolase 1
Protein EMBRYO DEFECTIVE 1276
Gene namesi
Name:AAT1
Synonyms:EMB1276
Ordered Locus Names:At5g48230
ORF Names:MIF21.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G48230.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • peroxisome Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Acetyl-CoA acetyltransferase, cytosolic 1PRO_0000206411Add
BLAST

Proteomic databases

PaxDbiQ8S4Y1.
PRIDEiQ8S4Y1.

Expressioni

Gene expression databases

ExpressionAtlasiQ8S4Y1. baseline and differential.
GenevisibleiQ8S4Y1. AT.

Interactioni

Protein-protein interaction databases

BioGridi20123. 4 interactions.
IntActiQ8S4Y1. 1 interaction.
MINTiMINT-8066749.
STRINGi3702.AT5G48230.2.

Structurei

3D structure databases

ProteinModelPortaliQ8S4Y1.
SMRiQ8S4Y1. Positions 11-403.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni232 – 2343Coenzyme A bindingBy similarity

Sequence similaritiesi

Belongs to the thiolase family.Curated

Phylogenomic databases

eggNOGiKOG1390. Eukaryota.
COG0183. LUCA.
HOGENOMiHOG000012238.
InParanoidiQ8S4Y1.
KOiK00626.
OMAiGPRNAKP.
PhylomeDBiQ8S4Y1.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
InterProiIPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8S4Y1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAHTSESVNP RDVCIVGVAR TPMGGFLGSL SSLPATKLGS LAIAAALKRA
60 70 80 90 100
NVDPALVQEV VFGNVLSANL GQAPARQAAL GAGIPNSVIC TTVNKVCASG
110 120 130 140 150
MKAVMIAAQS IQLGINDVVV AGGMESMSNT PKYLAEARKG SRFGHDSLVD
160 170 180 190 200
GMLKDGLWDV YNDCGMGSCA ELCAEKFQIT REQQDDYAVQ SFERGIAAQE
210 220 230 240 250
AGAFTWEIVP VEVSGGRGRP STIVDKDEGL GKFDAAKLRK LRPSFKENGG
260 270 280 290 300
TVTAGNASSI SDGAAALVLV SGEKALQLGL LVLAKIKGYG DAAQEPEFFT
310 320 330 340 350
TAPALAIPKA IAHAGLESSQ VDYYEINEAF AVVALANQKL LGIAPEKVNV
360 370 380 390 400
NGGAVSLGHP LGCSGARILI TLLGILKKRN GKYGVGGVCN GGGGASALVL

ELL
Length:403
Mass (Da):41,412
Last modified:June 1, 2002 - v1
Checksum:i113A349257F7FC98
GO
Isoform 2 (identifier: Q8S4Y1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: MAHTSESVNPR → MNVDES

Note: May be due to a competing donor splice site. No experimental confirmation available.
Show »
Length:398
Mass (Da):40,877
Checksum:i0BE1C6E1AF249D5F
GO

Sequence cautioni

The sequence BAA97003.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1111MAHTSESVNPR → MNVDES in isoform 2. 1 PublicationVSP_015461Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF364059 mRNA. Translation: AAM00280.1.
AB023039 Genomic DNA. Translation: BAA97003.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95638.1.
CP002688 Genomic DNA. Translation: AED95639.1.
AY059736 mRNA. Translation: AAL24148.1.
AY091271 mRNA. Translation: AAM14210.1.
RefSeqiNP_568694.2. NM_124198.3. [Q8S4Y1-1]
NP_851154.1. NM_180823.3. [Q8S4Y1-2]
UniGeneiAt.24167.
At.49143.

Genome annotation databases

EnsemblPlantsiAT5G48230.2; AT5G48230.2; AT5G48230. [Q8S4Y1-1]
GeneIDi834876.
KEGGiath:AT5G48230.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF364059 mRNA. Translation: AAM00280.1.
AB023039 Genomic DNA. Translation: BAA97003.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95638.1.
CP002688 Genomic DNA. Translation: AED95639.1.
AY059736 mRNA. Translation: AAL24148.1.
AY091271 mRNA. Translation: AAM14210.1.
RefSeqiNP_568694.2. NM_124198.3. [Q8S4Y1-1]
NP_851154.1. NM_180823.3. [Q8S4Y1-2]
UniGeneiAt.24167.
At.49143.

3D structure databases

ProteinModelPortaliQ8S4Y1.
SMRiQ8S4Y1. Positions 11-403.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20123. 4 interactions.
IntActiQ8S4Y1. 1 interaction.
MINTiMINT-8066749.
STRINGi3702.AT5G48230.2.

Proteomic databases

PaxDbiQ8S4Y1.
PRIDEiQ8S4Y1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G48230.2; AT5G48230.2; AT5G48230. [Q8S4Y1-1]
GeneIDi834876.
KEGGiath:AT5G48230.

Organism-specific databases

TAIRiAT5G48230.

Phylogenomic databases

eggNOGiKOG1390. Eukaryota.
COG0183. LUCA.
HOGENOMiHOG000012238.
InParanoidiQ8S4Y1.
KOiK00626.
OMAiGPRNAKP.
PhylomeDBiQ8S4Y1.

Enzyme and pathway databases

UniPathwayiUPA00058; UER00101.
BioCyciARA:GQT-183-MONOMER.
BRENDAi2.3.1.9. 399.
ReactomeiR-ATH-70895. Branched-chain amino acid catabolism.
R-ATH-77108. Utilization of Ketone Bodies.
R-ATH-77111. Synthesis of Ketone Bodies.

Miscellaneous databases

PROiQ8S4Y1.

Gene expression databases

ExpressionAtlasiQ8S4Y1. baseline and differential.
GenevisibleiQ8S4Y1. AT.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
InterProiIPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis thaliana mRNA encoding cytosolic acetoacetyl-CoA thiolase, the first enzyme of the mevalonate pathway for isoprenoid biosynthesis."
    Ahumada I., Boronat A., Campos N.
    Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.

Entry informationi

Entry nameiTHIC1_ARATH
AccessioniPrimary (citable) accession number: Q8S4Y1
Secondary accession number(s): Q93YW6, Q9LUB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2002
Last modified: May 11, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.