Q8S4Y1 (THIC1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-CoA acetyltransferase, cytosolic 1
EC=2.3.1.9

Alternative name(s):
Cytosolic acetoacetyl-CoA thiolase 1
Short name=Thiolase 1
Protein EMBRYO DEFECTIVE 1276

Gene names

Name:	AAT1
Synonyms:	EMB1276
Ordered Locus Names:	At5g48230
ORF Names:	MIF21.12

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 1/3.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the thiolase family.
Sequence caution	The sequence BAA97003.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding Potassium
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: TAIR peroxisome Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8S4Y1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8S4Y1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: MAHTSESVNPR → MNVDES

Note: May be due to a competing donor splice site. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Acetyl-CoA acetyltransferase, cytosolic 1		PRO_0000206411
Regions
	Region	232 – 234	3	Coenzyme A binding By similarity
Sites
	Active site	97	1	Acyl-thioester intermediate By similarity
	Active site	359	1	Proton acceptor By similarity
	Active site	389	1	Proton acceptor By similarity
	Metal binding	254	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	255	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	257	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	355	1	Potassium; via carbonyl oxygen By similarity
	Binding site	237	1	Coenzyme A By similarity
	Binding site	258	1	Coenzyme A By similarity
Natural variations
	Alternative sequence	1 – 11	11	MAHTSESVNPR → MNVDES in isoform 2.		VSP_015461

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 113A349257F7FC98	FASTA	403	41,412
	10 20 30 40 50 60 MAHTSESVNP RDVCIVGVAR TPMGGFLGSL SSLPATKLGS LAIAAALKRA NVDPALVQEV 70 80 90 100 110 120 VFGNVLSANL GQAPARQAAL GAGIPNSVIC TTVNKVCASG MKAVMIAAQS IQLGINDVVV 130 140 150 160 170 180 AGGMESMSNT PKYLAEARKG SRFGHDSLVD GMLKDGLWDV YNDCGMGSCA ELCAEKFQIT 190 200 210 220 230 240 REQQDDYAVQ SFERGIAAQE AGAFTWEIVP VEVSGGRGRP STIVDKDEGL GKFDAAKLRK 250 260 270 280 290 300 LRPSFKENGG TVTAGNASSI SDGAAALVLV SGEKALQLGL LVLAKIKGYG DAAQEPEFFT 310 320 330 340 350 360 TAPALAIPKA IAHAGLESSQ VDYYEINEAF AVVALANQKL LGIAPEKVNV NGGAVSLGHP 370 380 390 400 LGCSGARILI TLLGILKKRN GKYGVGGVCN GGGGASALVL ELL
	Isoform 2 [UniParc]. Checksum: 0BE1C6E1AF249D5F	FASTA	398	40,877
	10 20 30 40 50 60 MNVDESDVCI VGVARTPMGG FLGSLSSLPA TKLGSLAIAA ALKRANVDPA LVQEVVFGNV 70 80 90 100 110 120 LSANLGQAPA RQAALGAGIP NSVICTTVNK VCASGMKAVM IAAQSIQLGI NDVVVAGGME 130 140 150 160 170 180 SMSNTPKYLA EARKGSRFGH DSLVDGMLKD GLWDVYNDCG MGSCAELCAE KFQITREQQD 190 200 210 220 230 240 DYAVQSFERG IAAQEAGAFT WEIVPVEVSG GRGRPSTIVD KDEGLGKFDA AKLRKLRPSF 250 260 270 280 290 300 KENGGTVTAG NASSISDGAA ALVLVSGEKA LQLGLLVLAK IKGYGDAAQE PEFFTTAPAL 310 320 330 340 350 360 AIPKAIAHAG LESSQVDYYE INEAFAVVAL ANQKLLGIAP EKVNVNGGAV SLGHPLGCSG 370 380 390 ARILITLLGI LKKRNGKYGV GGVCNGGGGA SALVLELL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis thaliana mRNA encoding cytosolic acetoacetyl-CoA thiolase, the first enzyme of the mevalonate pathway for isoprenoid biosynthesis." Ahumada I., Boronat A., Campos N. Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF364059 mRNA. Translation: AAM00280.1. AB023039 Genomic DNA. Translation: BAA97003.1. Sequence problems. CP002688 Genomic DNA. Translation: AED95638.1. CP002688 Genomic DNA. Translation: AED95639.1. AY059736 mRNA. Translation: AAL24148.1. AY091271 mRNA. Translation: AAM14210.1.
IPI	IPI00531992. IPI00543299.
RefSeq	NP_568694.2. NM_124198.3. NP_851154.1. NM_180823.3.
UniGene	At.24167. At.49143.
3D structure databases
ProteinModelPortal	Q8S4Y1.
SMR	Q8S4Y1. Positions 6-403.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8S4Y1. 2 interactions.
STRING	Q8S4Y1.
Proteomic databases
PRIDE	Q8S4Y1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G48230.2; AT5G48230.2; AT5G48230.
GeneID	834876.
GenomeReviews	Gene locus AT5G48230 in contig BA000015_GR.
KEGG	ath:AT5G48230.
NMPDR	fig|3702.1.peg.26646.
Organism-specific databases
TAIR	At5g48230.
Phylogenomic databases
eggNOG	KOG1390.
GeneTree	EPGT00050000003137.
HOGENOM	HBG370930.
InParanoid	Q8S4Y1.
OMA	AFTWEIV.
PhylomeDB	Q8S4Y1.
ProtClustDB	PLN02644.
Gene expression databases
Genevestigator	Q8S4Y1.
GermOnline	AT5G48230. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 4 hits.
KO	K00626.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THIC1_ARATH

Accession

Primary (citable) accession number: Q8S4Y1
Secondary accession number(s): Q93YW6, Q9LUB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program