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Q8S4W7 (GAI1_VITVI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DELLA protein GAI1
Alternative name(s):
Gibberellic acid-insensitive mutant protein 1
VvGAI1
Gene names
Name:GAI1
OrganismVitis vinifera (Grape)
Taxonomic identifier29760 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis

Protein attributes

Sequence length590 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway.

Subcellular location

Nucleus By similarity.

Domain

The DELLA motif is required for its GA-induced degradation By similarity.

Post-translational modification

Phosphorylated By similarity.

Ubiquitinated. Upon GA application it is ubiquitinated, leading to its subsequent degradation By similarity.

Polymorphism

In cv. Pinot Meunier, 1 of the 3 cultivars used for the sparkling wine Champagne, plants are genetically indistinguishable from Pinot noir in most cells, but their outer layer, the 'L1' epidermal cell layer, is different: cv. Pinot Meunier has a furry surface on its leaves whereas Pinot noir does not. This is due to the Ser-387 variant that is present only in cells of the L1 layer that causes this difference, demonstrating that GA inhibits flowering in grapewine.

Sequence similarities

Belongs to the GRAS family. DELLA subfamily.

Ontologies

Keywords
   Biological processGibberellin signaling pathway
Transcription
Transcription regulation
   Cellular componentNucleus
   Molecular functionRepressor
   PTMPhosphoprotein
Ubl conjugation
Gene Ontology (GO)
   Biological_processgibberellic acid mediated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

hyperosmotic salinity response

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

jasmonic acid mediated signaling pathway

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

negative regulation of gibberellic acid mediated signaling pathway

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

negative regulation of seed germination

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

phloem transport

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

regulation of nitrogen utilization

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

regulation of protein catabolic process

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

regulation of reactive oxygen species metabolic process

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

regulation of seed dormancy process

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

response to abscisic acid

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to ethylene

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to far red light

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

salicylic acid mediated signaling pathway

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 590590DELLA protein GAI1
PRO_0000132248

Regions

Motif35 – 395DELLA motif
Motif315 – 3195VHIID
Motif413 – 4175LXXLL motif

Natural variations

Natural variant381L → H in Pinot Meunier L1 layer; induces the formation of influorescence instead of grapewine tendrils. Ref.1

Experimental info

Sequence conflict3871N → S in AAP20096. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8S4W7 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 0D9CF844C81C0001

FASTA59064,866
        10         20         30         40         50         60 
MKREYHHPHH PTCSTSPTGK GKMWDADPQQ DAGMDELLAV LGYNVKASDM AEVAQKLEQL 

        70         80         90        100        110        120 
EEVIVNAQED GLSHLASETV HYNPSDLSNW LGSMLSEFNP TPNCALDNPF LPPISPLDYT 

       130        140        150        160        170        180 
NCSTQPKQEP SIFDSPSLDY DLKAIPGKAL YSHIEQPPQQ PPAPPLYQRD NKRLKPTTSA 

       190        200        210        220        230        240 
TANSVSSVIG GWGVPTESAR PVVLVDSQET GIRLVHTLMA CAEAVQQENL KLAEALVKQI 

       250        260        270        280        290        300 
GFLAVSQAGA MRKVATYFAE GLARRIYRLY PDKPLDSSFS DILQMHFYET CPYLKFAHFT 

       310        320        330        340        350        360 
ANQAILEAFE GKKRVHVIDF SMKQGMQWPA LMQALALRPG GPPSFRLTGI GPPSTDNTDH 

       370        380        390        400        410        420 
LHEVGWKLAQ LAETIHVEFE YRGFVANSLA DLDASMLELR DGESVAVNSV FELHSLLARP 

       430        440        450        460        470        480 
GGIERVLSAV KDMKPDIVTI VEQEANHNGP VFLDRFTESL HYYSTLFDSL EGCGVSPVNT 

       490        500        510        520        530        540 
QDKLMSEVYL GQQICNVVAC EGPERVERHE TLAQWRARLG SAGFDPVNLG SNAFKQASML 

       550        560        570        580        590 
LALFAGGDGY RVEENNGCLM LGWHTRPLIA TSAWQLANKP ALPSSTPASN 

« Hide

References

[1]"Association of dwarfism and floral induction with a grape 'green revolution' mutation."
Boss P.K., Thomas M.R.
Nature 416:847-850(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT HIS-38.
Strain: cv. Pinot Meunier.
[2]Joly D., Perrin M., Gertz C., Masson J.E.
Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 294-440.
Strain: cv. Riesling.

Web resources

Protein Spotlight

All things dwarfed and beautiful - Issue 70 of May 2006

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF378125 Genomic DNA. Translation: AAM19210.1.
AY256862 mRNA. Translation: AAP20096.1.
RefSeqXP_002284648.1. XM_002284612.2.
UniGeneVvi.134.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100232937.
KEGGvvi:100232937.

Phylogenomic databases

KOK14494.

Family and domain databases

InterProIPR021914. TF_DELLA_N.
IPR005202. TF_GRAS.
[Graphical view]
PfamPF12041. DELLA. 1 hit.
PF03514. GRAS. 1 hit.
[Graphical view]
PROSITEPS50985. GRAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGAI1_VITVI
AccessionPrimary (citable) accession number: Q8S4W7
Secondary accession number(s): Q84MI8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries