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Q8S4W7

- GAI1_VITVI

UniProt

Q8S4W7 - GAI1_VITVI

Protein

DELLA protein GAI1

Gene

GAI1

Organism
Vitis vinifera (Grape)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway.

    GO - Biological processi

    1. gibberellic acid mediated signaling pathway Source: UniProtKB-KW
    2. hyperosmotic salinity response Source: EnsemblPlants/Gramene
    3. jasmonic acid mediated signaling pathway Source: EnsemblPlants/Gramene
    4. negative regulation of gibberellic acid mediated signaling pathway Source: EnsemblPlants/Gramene
    5. negative regulation of seed germination Source: EnsemblPlants/Gramene
    6. phloem transport Source: EnsemblPlants/Gramene
    7. regulation of nitrogen utilization Source: EnsemblPlants/Gramene
    8. regulation of protein catabolic process Source: EnsemblPlants/Gramene
    9. regulation of reactive oxygen species metabolic process Source: EnsemblPlants/Gramene
    10. regulation of seed dormancy process Source: EnsemblPlants/Gramene
    11. regulation of transcription, DNA-templated Source: UniProtKB-KW
    12. response to abscisic acid Source: EnsemblPlants/Gramene
    13. response to ethylene Source: EnsemblPlants/Gramene
    14. response to far red light Source: EnsemblPlants/Gramene
    15. salicylic acid mediated signaling pathway Source: EnsemblPlants/Gramene
    16. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Gibberellin signaling pathway, Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DELLA protein GAI1
    Alternative name(s):
    Gibberellic acid-insensitive mutant protein 1
    VvGAI1
    Gene namesi
    Name:GAI1
    OrganismiVitis vinifera (Grape)
    Taxonomic identifieri29760 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 590590DELLA protein GAI1PRO_0000132248Add
    BLAST

    Post-translational modificationi

    Phosphorylated.By similarity
    Ubiquitinated. Upon GA application it is ubiquitinated, leading to its subsequent degradation By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi35 – 395DELLA motif
    Motifi315 – 3195VHIID
    Motifi413 – 4175LXXLL motif

    Domaini

    The DELLA motif is required for its GA-induced degradation.By similarity

    Sequence similaritiesi

    Belongs to the GRAS family. DELLA subfamily.Curated

    Phylogenomic databases

    KOiK14494.

    Family and domain databases

    InterProiIPR021914. TF_DELLA_N.
    IPR005202. TF_GRAS.
    [Graphical view]
    PfamiPF12041. DELLA. 1 hit.
    PF03514. GRAS. 1 hit.
    [Graphical view]
    PROSITEiPS50985. GRAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8S4W7-1 [UniParc]FASTAAdd to Basket

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    MKREYHHPHH PTCSTSPTGK GKMWDADPQQ DAGMDELLAV LGYNVKASDM    50
    AEVAQKLEQL EEVIVNAQED GLSHLASETV HYNPSDLSNW LGSMLSEFNP 100
    TPNCALDNPF LPPISPLDYT NCSTQPKQEP SIFDSPSLDY DLKAIPGKAL 150
    YSHIEQPPQQ PPAPPLYQRD NKRLKPTTSA TANSVSSVIG GWGVPTESAR 200
    PVVLVDSQET GIRLVHTLMA CAEAVQQENL KLAEALVKQI GFLAVSQAGA 250
    MRKVATYFAE GLARRIYRLY PDKPLDSSFS DILQMHFYET CPYLKFAHFT 300
    ANQAILEAFE GKKRVHVIDF SMKQGMQWPA LMQALALRPG GPPSFRLTGI 350
    GPPSTDNTDH LHEVGWKLAQ LAETIHVEFE YRGFVANSLA DLDASMLELR 400
    DGESVAVNSV FELHSLLARP GGIERVLSAV KDMKPDIVTI VEQEANHNGP 450
    VFLDRFTESL HYYSTLFDSL EGCGVSPVNT QDKLMSEVYL GQQICNVVAC 500
    EGPERVERHE TLAQWRARLG SAGFDPVNLG SNAFKQASML LALFAGGDGY 550
    RVEENNGCLM LGWHTRPLIA TSAWQLANKP ALPSSTPASN 590
    Length:590
    Mass (Da):64,866
    Last modified:June 1, 2002 - v1
    Checksum:i0D9CF844C81C0001
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti387 – 3871N → S in AAP20096. 1 PublicationCurated

    Polymorphismi

    In cv. Pinot Meunier, 1 of the 3 cultivars used for the sparkling wine Champagne, plants are genetically indistinguishable from Pinot noir in most cells, but their outer layer, the 'L1' epidermal cell layer, is different: cv. Pinot Meunier has a furry surface on its leaves whereas Pinot noir does not. This is due to the Ser-387 variant that is present only in cells of the L1 layer that causes this difference, demonstrating that GA inhibits flowering in grapewine.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti38 – 381L → H in Pinot Meunier L1 layer; induces the formation of influorescence instead of grapewine tendrils. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF378125 Genomic DNA. Translation: AAM19210.1.
    AY256862 mRNA. Translation: AAP20096.1.
    RefSeqiXP_002284648.1. XM_002284612.2.
    UniGeneiVvi.134.

    Genome annotation databases

    GeneIDi100232937.
    KEGGivvi:100232937.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    All things dwarfed and beautiful - Issue 70 of May 2006

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF378125 Genomic DNA. Translation: AAM19210.1 .
    AY256862 mRNA. Translation: AAP20096.1 .
    RefSeqi XP_002284648.1. XM_002284612.2.
    UniGenei Vvi.134.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100232937.
    KEGGi vvi:100232937.

    Phylogenomic databases

    KOi K14494.

    Family and domain databases

    InterProi IPR021914. TF_DELLA_N.
    IPR005202. TF_GRAS.
    [Graphical view ]
    Pfami PF12041. DELLA. 1 hit.
    PF03514. GRAS. 1 hit.
    [Graphical view ]
    PROSITEi PS50985. GRAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Association of dwarfism and floral induction with a grape 'green revolution' mutation."
      Boss P.K., Thomas M.R.
      Nature 416:847-850(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT HIS-38.
      Strain: cv. Pinot Meunier.
    2. Joly D., Perrin M., Gertz C., Masson J.E.
      Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 294-440.
      Strain: cv. Riesling.

    Entry informationi

    Entry nameiGAI1_VITVI
    AccessioniPrimary (citable) accession number: Q8S4W7
    Secondary accession number(s): Q84MI8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3