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Q8S4W7

- GAI1_VITVI

UniProt

Q8S4W7 - GAI1_VITVI

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Protein

DELLA protein GAI1

Gene

GAI1

Organism
Vitis vinifera (Grape)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway.

GO - Biological processi

  1. gibberellic acid mediated signaling pathway Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Gibberellin signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
DELLA protein GAI1
Alternative name(s):
Gibberellic acid-insensitive mutant protein 1
VvGAI1
Gene namesi
Name:GAI1
OrganismiVitis vinifera (Grape)
Taxonomic identifieri29760 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 590590DELLA protein GAI1PRO_0000132248Add
BLAST

Post-translational modificationi

Phosphorylated.By similarity
Ubiquitinated. Upon GA application it is ubiquitinated, leading to its subsequent degradation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi35 – 395DELLA motif
Motifi315 – 3195VHIID
Motifi413 – 4175LXXLL motif

Domaini

The DELLA motif is required for its GA-induced degradation.By similarity

Sequence similaritiesi

Belongs to the GRAS family. DELLA subfamily.Curated

Phylogenomic databases

KOiK14494.

Family and domain databases

InterProiIPR021914. TF_DELLA_N.
IPR005202. TF_GRAS.
[Graphical view]
PfamiPF12041. DELLA. 1 hit.
PF03514. GRAS. 1 hit.
[Graphical view]
PROSITEiPS50985. GRAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8S4W7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKREYHHPHH PTCSTSPTGK GKMWDADPQQ DAGMDELLAV LGYNVKASDM
60 70 80 90 100
AEVAQKLEQL EEVIVNAQED GLSHLASETV HYNPSDLSNW LGSMLSEFNP
110 120 130 140 150
TPNCALDNPF LPPISPLDYT NCSTQPKQEP SIFDSPSLDY DLKAIPGKAL
160 170 180 190 200
YSHIEQPPQQ PPAPPLYQRD NKRLKPTTSA TANSVSSVIG GWGVPTESAR
210 220 230 240 250
PVVLVDSQET GIRLVHTLMA CAEAVQQENL KLAEALVKQI GFLAVSQAGA
260 270 280 290 300
MRKVATYFAE GLARRIYRLY PDKPLDSSFS DILQMHFYET CPYLKFAHFT
310 320 330 340 350
ANQAILEAFE GKKRVHVIDF SMKQGMQWPA LMQALALRPG GPPSFRLTGI
360 370 380 390 400
GPPSTDNTDH LHEVGWKLAQ LAETIHVEFE YRGFVANSLA DLDASMLELR
410 420 430 440 450
DGESVAVNSV FELHSLLARP GGIERVLSAV KDMKPDIVTI VEQEANHNGP
460 470 480 490 500
VFLDRFTESL HYYSTLFDSL EGCGVSPVNT QDKLMSEVYL GQQICNVVAC
510 520 530 540 550
EGPERVERHE TLAQWRARLG SAGFDPVNLG SNAFKQASML LALFAGGDGY
560 570 580 590
RVEENNGCLM LGWHTRPLIA TSAWQLANKP ALPSSTPASN
Length:590
Mass (Da):64,866
Last modified:June 1, 2002 - v1
Checksum:i0D9CF844C81C0001
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti387 – 3871N → S in AAP20096. 1 PublicationCurated

Polymorphismi

In cv. Pinot Meunier, 1 of the 3 cultivars used for the sparkling wine Champagne, plants are genetically indistinguishable from Pinot noir in most cells, but their outer layer, the 'L1' epidermal cell layer, is different: cv. Pinot Meunier has a furry surface on its leaves whereas Pinot noir does not. This is due to the Ser-387 variant that is present only in cells of the L1 layer that causes this difference, demonstrating that GA inhibits flowering in grapewine.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti38 – 381L → H in Pinot Meunier L1 layer; induces the formation of influorescence instead of grapewine tendrils. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF378125 Genomic DNA. Translation: AAM19210.1.
AY256862 mRNA. Translation: AAP20096.1.
RefSeqiXP_002284648.1. XM_002284612.2.
UniGeneiVvi.134.

Genome annotation databases

GeneIDi100232937.
KEGGivvi:100232937.

Cross-referencesi

Web resourcesi

Protein Spotlight

All things dwarfed and beautiful - Issue 70 of May 2006

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF378125 Genomic DNA. Translation: AAM19210.1 .
AY256862 mRNA. Translation: AAP20096.1 .
RefSeqi XP_002284648.1. XM_002284612.2.
UniGenei Vvi.134.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100232937.
KEGGi vvi:100232937.

Phylogenomic databases

KOi K14494.

Family and domain databases

InterProi IPR021914. TF_DELLA_N.
IPR005202. TF_GRAS.
[Graphical view ]
Pfami PF12041. DELLA. 1 hit.
PF03514. GRAS. 1 hit.
[Graphical view ]
PROSITEi PS50985. GRAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Association of dwarfism and floral induction with a grape 'green revolution' mutation."
    Boss P.K., Thomas M.R.
    Nature 416:847-850(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT HIS-38.
    Strain: cv. Pinot Meunier.
  2. Joly D., Perrin M., Gertz C., Masson J.E.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 294-440.
    Strain: cv. Riesling.

Entry informationi

Entry nameiGAI1_VITVI
AccessioniPrimary (citable) accession number: Q8S4W7
Secondary accession number(s): Q84MI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3