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Protein

DELLA protein GAI1

Gene

GAI1

Organism
Vitis vinifera (Grape)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor
Biological processGibberellin signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
DELLA protein GAI1
Alternative name(s):
Gibberellic acid-insensitive mutant protein 1
VvGAI1
Gene namesi
Name:GAI1
OrganismiVitis vinifera (Grape)
Taxonomic identifieri29760 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001322481 – 590DELLA protein GAI1Add BLAST590

Post-translational modificationi

Phosphorylated.By similarity
Ubiquitinated. Upon GA application it is ubiquitinated, leading to its subsequent degradation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Expressioni

Gene expression databases

ExpressionAtlasiQ8S4W7 baseline and differential

Structurei

3D structure databases

SMRiQ8S4W7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini205 – 575GRASPROSITE-ProRule annotationAdd BLAST371

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 266Leucine repeat I (LRI)PROSITE-ProRule annotationAdd BLAST55
Regioni284 – 349VHIIDPROSITE-ProRule annotationAdd BLAST66
Regioni363 – 395Leucine repeat II (LRII)PROSITE-ProRule annotationAdd BLAST33
Regioni405 – 496PFYREPROSITE-ProRule annotationAdd BLAST92
Regioni499 – 575SAWPROSITE-ProRule annotationAdd BLAST77

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi35 – 39DELLA motif5
Motifi315 – 319VHIIDPROSITE-ProRule annotation5
Motifi413 – 417LXXLL motifPROSITE-ProRule annotation5

Domaini

The DELLA motif is required for its GA-induced degradation.By similarity

Sequence similaritiesi

Belongs to the GRAS family. DELLA subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IQYH Eukaryota
ENOG410YBFY LUCA
KOiK14494
OrthoDBiEOG0936075R

Family and domain databases

Gene3Di1.10.10.1290, 1 hit
InterProiView protein in InterPro
IPR038088 DELLA_N_sf
IPR030006 TF_DELLA
IPR021914 TF_DELLA_N
IPR005202 TF_GRAS
PANTHERiPTHR31636:SF47 PTHR31636:SF47, 1 hit
PfamiView protein in Pfam
PF12041 DELLA, 1 hit
PF03514 GRAS, 1 hit
PROSITEiView protein in PROSITE
PS50985 GRAS, 1 hit

Sequencei

Sequence statusi: Complete.

Q8S4W7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKREYHHPHH PTCSTSPTGK GKMWDADPQQ DAGMDELLAV LGYNVKASDM
60 70 80 90 100
AEVAQKLEQL EEVIVNAQED GLSHLASETV HYNPSDLSNW LGSMLSEFNP
110 120 130 140 150
TPNCALDNPF LPPISPLDYT NCSTQPKQEP SIFDSPSLDY DLKAIPGKAL
160 170 180 190 200
YSHIEQPPQQ PPAPPLYQRD NKRLKPTTSA TANSVSSVIG GWGVPTESAR
210 220 230 240 250
PVVLVDSQET GIRLVHTLMA CAEAVQQENL KLAEALVKQI GFLAVSQAGA
260 270 280 290 300
MRKVATYFAE GLARRIYRLY PDKPLDSSFS DILQMHFYET CPYLKFAHFT
310 320 330 340 350
ANQAILEAFE GKKRVHVIDF SMKQGMQWPA LMQALALRPG GPPSFRLTGI
360 370 380 390 400
GPPSTDNTDH LHEVGWKLAQ LAETIHVEFE YRGFVANSLA DLDASMLELR
410 420 430 440 450
DGESVAVNSV FELHSLLARP GGIERVLSAV KDMKPDIVTI VEQEANHNGP
460 470 480 490 500
VFLDRFTESL HYYSTLFDSL EGCGVSPVNT QDKLMSEVYL GQQICNVVAC
510 520 530 540 550
EGPERVERHE TLAQWRARLG SAGFDPVNLG SNAFKQASML LALFAGGDGY
560 570 580 590
RVEENNGCLM LGWHTRPLIA TSAWQLANKP ALPSSTPASN
Length:590
Mass (Da):64,866
Last modified:June 1, 2002 - v1
Checksum:i0D9CF844C81C0001
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti387N → S in AAP20096 (Ref. 2) Curated1

Polymorphismi

In cv. Pinot Meunier, 1 of the 3 cultivars used for the sparkling wine Champagne, plants are genetically indistinguishable from Pinot noir in most cells, but their outer layer, the 'L1' epidermal cell layer, is different: cv. Pinot Meunier has a furry surface on its leaves whereas Pinot noir does not. This is due to the Ser-387 variant that is present only in cells of the L1 layer that causes this difference, demonstrating that GA inhibits flowering in grapewine.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti38L → H in Pinot Meunier L1 layer; induces the formation of influorescence instead of grapewine tendrils. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF378125 Genomic DNA Translation: AAM19210.1
AY256862 mRNA Translation: AAP20096.1
RefSeqiXP_002284648.1, XM_002284612.4
UniGeneiVvi.134

Genome annotation databases

EnsemblPlantsiVIT_01s0011g05260.t01; VIT_01s0011g05260.t01; VIT_01s0011g05260
GeneIDi100232937
GrameneiVIT_01s0011g05260.t01; VIT_01s0011g05260.t01; VIT_01s0011g05260
KEGGivvi:100232937

Similar proteinsi

Entry informationi

Entry nameiGAI1_VITVI
AccessioniPrimary (citable) accession number: Q8S4W7
Secondary accession number(s): Q84MI8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health