Q8S4W7 (GAI1_VITVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DELLA protein GAI1

Alternative name(s):
Gibberellic acid-insensitive mutant protein 1
VvGAI1

Gene names

Name:

GAI1

Organism

Vitis vinifera (Grape)

Taxonomic identifier

29760 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › rosids incertae sedis › Vitales › Vitaceae › Vitis

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway.
Subcellular location	Nucleus By similarity.
Domain	The DELLA motif is required for its GA-induced degradation By similarity.
Post-translational modification	Phosphorylated By similarity. Ubiquitinated. Upon GA application it is ubiquitinated, leading to its subsequent degradation By similarity.
Polymorphism	In cv. Pinot Meunier, 1 of the 3 cultivars used for the sparkling wine Champagne, plants are genetically indistinguishable from Pinot noir in most cells, but their outer layer, the 'L1' epidermal cell layer, is different: cv. Pinot Meunier has a furry surface on its leaves whereas Pinot noir does not. This is due to the Ser-387 variant that is present only in cells of the L1 layer that causes this difference, demonstrating that GA inhibits flowering in grapewine.
Sequence similarities	Belongs to the GRAS family. DELLA subfamily.

Ontologies

Keywords
Biological process	Gibberellin signaling pathway Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Repressor
PTM	Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological_process	gibberellic acid mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW hyperosmotic salinity response Inferred from electronic annotation. Source: EnsemblPlants/Gramene jasmonic acid mediated signaling pathway Inferred from electronic annotation. Source: EnsemblPlants/Gramene negative regulation of gibberellic acid mediated signaling pathway Inferred from electronic annotation. Source: EnsemblPlants/Gramene negative regulation of seed germination Inferred from electronic annotation. Source: EnsemblPlants/Gramene phloem transport Inferred from electronic annotation. Source: EnsemblPlants/Gramene regulation of gene expression, epigenetic Inferred from electronic annotation. Source: EnsemblPlants/Gramene regulation of nitrogen utilization Inferred from electronic annotation. Source: EnsemblPlants/Gramene regulation of protein catabolic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene regulation of seed dormancy process Inferred from electronic annotation. Source: EnsemblPlants/Gramene regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to abscisic acid stimulus Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to ethylene stimulus Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to far red light Inferred from electronic annotation. Source: EnsemblPlants/Gramene salicylic acid mediated signaling pathway Inferred from electronic annotation. Source: EnsemblPlants/Gramene transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	DELLA protein GAI1		PRO_0000132248
Regions
	Motif	35 – 39	5	DELLA motif
	Motif	315 – 319	5	VHIID
	Motif	413 – 417	5	LXXLL motif
Natural variations
	Natural variant	38	1	L → H in Pinot Meunier L1 layer; induces the formation of influorescence instead of grapewine tendrils. Ref.1
Experimental info
	Sequence conflict	387	1	N → S in AAP20096. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8S4W7 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 0D9CF844C81C0001	FASTA	590	64,866
	10 20 30 40 50 60 MKREYHHPHH PTCSTSPTGK GKMWDADPQQ DAGMDELLAV LGYNVKASDM AEVAQKLEQL 70 80 90 100 110 120 EEVIVNAQED GLSHLASETV HYNPSDLSNW LGSMLSEFNP TPNCALDNPF LPPISPLDYT 130 140 150 160 170 180 NCSTQPKQEP SIFDSPSLDY DLKAIPGKAL YSHIEQPPQQ PPAPPLYQRD NKRLKPTTSA 190 200 210 220 230 240 TANSVSSVIG GWGVPTESAR PVVLVDSQET GIRLVHTLMA CAEAVQQENL KLAEALVKQI 250 260 270 280 290 300 GFLAVSQAGA MRKVATYFAE GLARRIYRLY PDKPLDSSFS DILQMHFYET CPYLKFAHFT 310 320 330 340 350 360 ANQAILEAFE GKKRVHVIDF SMKQGMQWPA LMQALALRPG GPPSFRLTGI GPPSTDNTDH 370 380 390 400 410 420 LHEVGWKLAQ LAETIHVEFE YRGFVANSLA DLDASMLELR DGESVAVNSV FELHSLLARP 430 440 450 460 470 480 GGIERVLSAV KDMKPDIVTI VEQEANHNGP VFLDRFTESL HYYSTLFDSL EGCGVSPVNT 490 500 510 520 530 540 QDKLMSEVYL GQQICNVVAC EGPERVERHE TLAQWRARLG SAGFDPVNLG SNAFKQASML 550 560 570 580 590 LALFAGGDGY RVEENNGCLM LGWHTRPLIA TSAWQLANKP ALPSSTPASN

References

[1]	"Association of dwarfism and floral induction with a grape 'green revolution' mutation." Boss P.K., Thomas M.R. Nature 416:847-850(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT HIS-38. Strain: cv. Pinot Meunier.
[2]	Joly D., Perrin M., Gertz C., Masson J.E. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 294-440. Strain: cv. Riesling.

Web resources

Protein Spotlight

All things dwarfed and beautiful - Issue 70 of May 2006

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF378125 Genomic DNA. Translation: AAM19210.1. AY256862 mRNA. Translation: AAP20096.1.
RefSeq	XP_002284648.1. XM_002284612.2.
UniGene	Vvi.134.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100232937.
KEGG	vvi:100232937.
Phylogenomic databases
KO	K14494.
ProtClustDB	CLSN2721346.
Gene expression databases
ArrayExpress	Q8S4W7.
Family and domain databases
InterPro	IPR021914. TF_DELLA_N. IPR005202. TF_GRAS. [Graphical view]
Pfam	PF12041. DELLA. 1 hit. PF03514. GRAS. 1 hit. [Graphical view]
PROSITE	PS50985. GRAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAI1_VITVI

Accession

Primary (citable) accession number: Q8S4W7
Secondary accession number(s): Q84MI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	June 1, 2002
Last modified:	April 3, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program