Q8S4P5 (EZ2_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone-lysine N-methyltransferase EZ2
EC=2.1.1.43

Alternative name(s):
Enhancer of zeste protein 2

Gene names

Name:	EZ2
Synonyms:	MEZ2

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	894 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Polycomb group (PcG) protein. Catalytic subunit of some PcG multiprotein complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. PcG proteins are not required to initiate repression, but to maintain it during later stages of development By similarity.
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the histone-lysine methyltransferase family. EZ subfamily. Contains 1 CXC domain. Contains 1 SANT domain. Contains 1 SET domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Repressor Transferase
Gene Ontology (GO)
Biological_process	endosperm development Inferred from electronic annotation. Source: EnsemblPlants/Gramene histone lysine methylation Inferred from electronic annotation. Source: GOC regulation of gene expression by genetic imprinting Inferred from electronic annotation. Source: EnsemblPlants/Gramene regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	PcG protein complex Inferred from electronic annotation. Source: InterPro
Molecular_function	chromatin binding Inferred from electronic annotation. Source: InterPro histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC single-stranded RNA binding Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8S4P5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8S4P5-2)

Also known as: as1;

The sequence of this isoform differs from the canonical sequence as follows:
     342-894: Missing.

Isoform 3 (identifier: Q8S4P5-3)

Also known as: as2;

The sequence of this isoform differs from the canonical sequence as follows:
     625-894: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 894	894	Histone-lysine N-methyltransferase EZ2		PRO_0000213999
Regions
	Domain	527 – 577	51	SANT
	Domain	627 – 731	105	CXC
	Domain	745 – 865	121	SET
	Compositional bias	25 – 28	4	Poly-Ala
	Compositional bias	74 – 77	4	Poly-Ala
	Compositional bias	642 – 718	77	Cys-rich
Natural variations
	Alternative sequence	342 – 894	553	Missing in isoform 2.		VSP_007783
	Alternative sequence	625 – 894	270	Missing in isoform 3.		VSP_007784

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 9C2B04E6F80BA113	FASTA	894	99,979
	10 20 30 40 50 60 MASSSKASDS SQRSKRSDQG MGKDAAAASV VPIHANLTQL IRQVQSGRLA YIKEKLEVNR 70 80 90 100 110 120 KTLQRHSCSL FDVAAAAEVA SRGTDGGNAL SQRAAERQCG SDLANGIGER DVVSVQEENL 130 140 150 160 170 180 ATGTLALSSS GATAQRTIVR FVKLPLVEKI PPYTTWIFLD KNQRMADDQS VVGRRRIYYD 190 200 210 220 230 240 TVGNEALICS DSDEEIPEPE EEKHFFTKGE DHLIWRATQD HGLNQEVVNV LCQFIGATPS 250 260 270 280 290 300 EIEERSEVLF EKNEKHSGSS DKIESRLSLD KTMDAVLDSF DNLFCRRCLV FDCRLHGCSQ 310 320 330 340 350 360 NLVFPCEKQP YSFDPDENKK PCGHLCYLRF PQWREGFKEM HDDGLAGGAT YTMESGTASQ 370 380 390 400 410 420 RVDVNVMYES EDSNRQKGNI RSMTLVGTSG SKIISSVSAE ESTTTPSADI SETENVSSDL 430 440 450 460 470 480 PPSSLRKHKI SKHGPRYREH SPGKRQKVFT SDISFEGNIM NKLSIPEIRD TRLESRESGG 490 500 510 520 530 540 DKLRILDEST KKTSRKDMCG ESPATTMENV GRQSNKVSST KNFLESTLSC WSALERDLYL 550 560 570 580 590 600 KGIEIFGKNS CLIARNLLSG LKTCIEVANY MYNNGAAMAK RPLLNKSISG DFAENEQDYM 610 620 630 640 650 660 EQDMAARTRI YRRRGRNRKL KYTWKSAGHP TVRKRTDDGK QCYTQYSPCA CQQMCGKDCP 670 680 690 700 710 720 CADKGTCCEK YCGCSKSCKN KFRGCHCAKS QCRSRQCPCF AASRECDPDV CRNCWVSCGD 730 740 750 760 770 780 GSLGEPLARG DGYQCGNMKL LLKQQQRILL GRSDVAGWGA FIKNPVNKND YLGEYTGELI 790 800 810 820 830 840 SHKEADKRGK IYDRANSSFL FDLNDQYVLD AYRKGDKLKF ANHSSNPNCY AKVMLVAGDH 850 860 870 880 890 RVGIYAKEHI EASEELFYDY RYGPDQAPAW ARRPEGSKKD EASVSHRRAH KVAR
	Isoform 2 (as1) [UniParc]. Checksum: 0CFBD999C5BD4604	FASTA	341	38,167
	10 20 30 40 50 60 MASSSKASDS SQRSKRSDQG MGKDAAAASV VPIHANLTQL IRQVQSGRLA YIKEKLEVNR 70 80 90 100 110 120 KTLQRHSCSL FDVAAAAEVA SRGTDGGNAL SQRAAERQCG SDLANGIGER DVVSVQEENL 130 140 150 160 170 180 ATGTLALSSS GATAQRTIVR FVKLPLVEKI PPYTTWIFLD KNQRMADDQS VVGRRRIYYD 190 200 210 220 230 240 TVGNEALICS DSDEEIPEPE EEKHFFTKGE DHLIWRATQD HGLNQEVVNV LCQFIGATPS 250 260 270 280 290 300 EIEERSEVLF EKNEKHSGSS DKIESRLSLD KTMDAVLDSF DNLFCRRCLV FDCRLHGCSQ 310 320 330 340 NLVFPCEKQP YSFDPDENKK PCGHLCYLRF PQWREGFKEM H
	Isoform 3 (as2) [UniParc]. Checksum: D94D319FADF43A19	FASTA	624	69,732
	10 20 30 40 50 60 MASSSKASDS SQRSKRSDQG MGKDAAAASV VPIHANLTQL IRQVQSGRLA YIKEKLEVNR 70 80 90 100 110 120 KTLQRHSCSL FDVAAAAEVA SRGTDGGNAL SQRAAERQCG SDLANGIGER DVVSVQEENL 130 140 150 160 170 180 ATGTLALSSS GATAQRTIVR FVKLPLVEKI PPYTTWIFLD KNQRMADDQS VVGRRRIYYD 190 200 210 220 230 240 TVGNEALICS DSDEEIPEPE EEKHFFTKGE DHLIWRATQD HGLNQEVVNV LCQFIGATPS 250 260 270 280 290 300 EIEERSEVLF EKNEKHSGSS DKIESRLSLD KTMDAVLDSF DNLFCRRCLV FDCRLHGCSQ 310 320 330 340 350 360 NLVFPCEKQP YSFDPDENKK PCGHLCYLRF PQWREGFKEM HDDGLAGGAT YTMESGTASQ 370 380 390 400 410 420 RVDVNVMYES EDSNRQKGNI RSMTLVGTSG SKIISSVSAE ESTTTPSADI SETENVSSDL 430 440 450 460 470 480 PPSSLRKHKI SKHGPRYREH SPGKRQKVFT SDISFEGNIM NKLSIPEIRD TRLESRESGG 490 500 510 520 530 540 DKLRILDEST KKTSRKDMCG ESPATTMENV GRQSNKVSST KNFLESTLSC WSALERDLYL 550 560 570 580 590 600 KGIEIFGKNS CLIARNLLSG LKTCIEVANY MYNNGAAMAK RPLLNKSISG DFAENEQDYM 610 620 EQDMAARTRI YRRRGRNRKL KYTW

References

[1]

"Sequence relationships, conserved domains, and expression patterns for maize homologs of the Polycomb group genes E(z), esc, and E(Pc)." Springer N.M., Danilevskaya O.N., Hermon P., Helentjaris T.G., Phillips R.L., Kaeppler H.F., Kaeppler S.M. Plant Physiol. 128:1332-1345(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY, ALTERNATIVE SPLICING. Tissue: Seed.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF443597 mRNA. Translation: AAM13421.1.
RefSeq	NP_001105650.1. NM_001112180.1.
UniGene	Zm.10342.
3D structure databases
ProteinModelPortal	Q8S4P5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	542659.
KEGG	zma:542659.
Organism-specific databases
Gramene	Q8S4P5.
MaizeGDB	754843.
Phylogenomic databases
HOGENOM	HOG000083511.
KO	K11430.
Family and domain databases
InterPro	IPR026489. CXC_dom. IPR025778. Hist-Lys_N-MeTrfase_EZ. IPR001005. SANT/Myb. IPR001214. SET_dom. [Graphical view]
Pfam	PF00856. SET. 1 hit. [Graphical view]
SMART	SM00717. SANT. 1 hit. SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS51633. CXC. 1 hit. PS51293. SANT. False negative. PS50280. SET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EZ2_MAIZE

Accession

Primary (citable) accession number: Q8S4P5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	June 1, 2002
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program