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Protein

Histone-lysine N-methyltransferase EZ2

Gene

EZ2

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Polycomb group (PcG) protein. Catalytic subunit of some PcG multiprotein complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. PcG proteins are not required to initiate repression, but to maintain it during later stages of development (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].PROSITE-ProRule annotation

GO - Molecular functioni

  1. chromatin binding Source: InterPro
  2. histone-lysine N-methyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Repressor, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase EZ2 (EC:2.1.1.43)
Alternative name(s):
Enhancer of zeste protein 2
Gene namesi
Name:EZ2
Synonyms:MEZ2
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea
ProteomesiUP000007305 Componenti: Chromosome 9

Organism-specific databases

GrameneiQ8S4P5.
MaizeGDBi754843.

Subcellular locationi

  1. Nucleus Curated

GO - Cellular componenti

  1. PcG protein complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 894894Histone-lysine N-methyltransferase EZ2PRO_0000213999Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ8S4P5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini527 – 57751SANTAdd
BLAST
Domaini627 – 731105CXCPROSITE-ProRule annotationAdd
BLAST
Domaini746 – 861116SETPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi25 – 284Poly-Ala
Compositional biasi74 – 774Poly-Ala
Compositional biasi642 – 71877Cys-richAdd
BLAST

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. EZ subfamily.PROSITE-ProRule annotation
Contains 1 CXC domain.PROSITE-ProRule annotation
Contains 1 SANT domain.Curated
Contains 1 SET domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000083511.
KOiK11430.

Family and domain databases

InterProiIPR026489. CXC_dom.
IPR025778. Hist-Lys_N-MeTrfase_EZ.
IPR001005. SANT/Myb.
IPR001214. SET_dom.
[Graphical view]
PfamiPF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
SM00317. SET. 1 hit.
[Graphical view]
PROSITEiPS51633. CXC. 1 hit.
PS51576. SAM_MT43_EZ. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8S4P5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSSKASDS SQRSKRSDQG MGKDAAAASV VPIHANLTQL IRQVQSGRLA
60 70 80 90 100
YIKEKLEVNR KTLQRHSCSL FDVAAAAEVA SRGTDGGNAL SQRAAERQCG
110 120 130 140 150
SDLANGIGER DVVSVQEENL ATGTLALSSS GATAQRTIVR FVKLPLVEKI
160 170 180 190 200
PPYTTWIFLD KNQRMADDQS VVGRRRIYYD TVGNEALICS DSDEEIPEPE
210 220 230 240 250
EEKHFFTKGE DHLIWRATQD HGLNQEVVNV LCQFIGATPS EIEERSEVLF
260 270 280 290 300
EKNEKHSGSS DKIESRLSLD KTMDAVLDSF DNLFCRRCLV FDCRLHGCSQ
310 320 330 340 350
NLVFPCEKQP YSFDPDENKK PCGHLCYLRF PQWREGFKEM HDDGLAGGAT
360 370 380 390 400
YTMESGTASQ RVDVNVMYES EDSNRQKGNI RSMTLVGTSG SKIISSVSAE
410 420 430 440 450
ESTTTPSADI SETENVSSDL PPSSLRKHKI SKHGPRYREH SPGKRQKVFT
460 470 480 490 500
SDISFEGNIM NKLSIPEIRD TRLESRESGG DKLRILDEST KKTSRKDMCG
510 520 530 540 550
ESPATTMENV GRQSNKVSST KNFLESTLSC WSALERDLYL KGIEIFGKNS
560 570 580 590 600
CLIARNLLSG LKTCIEVANY MYNNGAAMAK RPLLNKSISG DFAENEQDYM
610 620 630 640 650
EQDMAARTRI YRRRGRNRKL KYTWKSAGHP TVRKRTDDGK QCYTQYSPCA
660 670 680 690 700
CQQMCGKDCP CADKGTCCEK YCGCSKSCKN KFRGCHCAKS QCRSRQCPCF
710 720 730 740 750
AASRECDPDV CRNCWVSCGD GSLGEPLARG DGYQCGNMKL LLKQQQRILL
760 770 780 790 800
GRSDVAGWGA FIKNPVNKND YLGEYTGELI SHKEADKRGK IYDRANSSFL
810 820 830 840 850
FDLNDQYVLD AYRKGDKLKF ANHSSNPNCY AKVMLVAGDH RVGIYAKEHI
860 870 880 890
EASEELFYDY RYGPDQAPAW ARRPEGSKKD EASVSHRRAH KVAR
Length:894
Mass (Da):99,979
Last modified:June 1, 2002 - v1
Checksum:i9C2B04E6F80BA113
GO
Isoform 2 (identifier: Q8S4P5-2) [UniParc]FASTAAdd to basket

Also known as: as1

The sequence of this isoform differs from the canonical sequence as follows:
     342-894: Missing.

Show »
Length:341
Mass (Da):38,167
Checksum:i0CFBD999C5BD4604
GO
Isoform 3 (identifier: Q8S4P5-3) [UniParc]FASTAAdd to basket

Also known as: as2

The sequence of this isoform differs from the canonical sequence as follows:
     625-894: Missing.

Show »
Length:624
Mass (Da):69,732
Checksum:iD94D319FADF43A19
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei342 – 894553Missing in isoform 2. 1 PublicationVSP_007783Add
BLAST
Alternative sequencei625 – 894270Missing in isoform 3. 1 PublicationVSP_007784Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF443597 mRNA. Translation: AAM13421.1.
RefSeqiNP_001105650.1. NM_001112180.1. [Q8S4P5-1]
UniGeneiZm.10342.

Genome annotation databases

EnsemblPlantsiGRMZM5G875502_T02; GRMZM5G875502_P02; GRMZM5G875502. [Q8S4P5-1]
GeneIDi542659.
KEGGizma:542659.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF443597 mRNA. Translation: AAM13421.1.
RefSeqiNP_001105650.1. NM_001112180.1. [Q8S4P5-1]
UniGeneiZm.10342.

3D structure databases

ProteinModelPortaliQ8S4P5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiGRMZM5G875502_T02; GRMZM5G875502_P02; GRMZM5G875502. [Q8S4P5-1]
GeneIDi542659.
KEGGizma:542659.

Organism-specific databases

GrameneiQ8S4P5.
MaizeGDBi754843.

Phylogenomic databases

HOGENOMiHOG000083511.
KOiK11430.

Family and domain databases

InterProiIPR026489. CXC_dom.
IPR025778. Hist-Lys_N-MeTrfase_EZ.
IPR001005. SANT/Myb.
IPR001214. SET_dom.
[Graphical view]
PfamiPF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
SM00317. SET. 1 hit.
[Graphical view]
PROSITEiPS51633. CXC. 1 hit.
PS51576. SAM_MT43_EZ. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence relationships, conserved domains, and expression patterns for maize homologs of the Polycomb group genes E(z), esc, and E(Pc)."
    Springer N.M., Danilevskaya O.N., Hermon P., Helentjaris T.G., Phillips R.L., Kaeppler H.F., Kaeppler S.M.
    Plant Physiol. 128:1332-1345(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
    Tissue: Seed.

Entry informationi

Entry nameiEZ2_MAIZE
AccessioniPrimary (citable) accession number: Q8S4P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: June 1, 2002
Last modified: March 4, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.