Reviewed, UniProtKB/Swiss-Prot Q8S4P4 (EZ3_MAIZE)

Names and origin

Protein names

Recommended name:
    Histone-lysine N-methyltransferase EZ3
    EC=2.1.1.43
Alternative name(s):
    Enhancer of zeste protein 3

Gene names

Name:	EZ3
Synonyms:	MEZ3

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	895 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Polycomb group (PcG) protein. Catalytic subunit of some PcG multiprotein complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. PcG proteins are not required to initiate repression, but to maintain it during later stages of development By similarity.
Catalytic activity	S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
Subcellular location	Nucleus Probable.
Tissue specificity	Widely expressed. Ref.1
Sequence similarities	Belongs to the histone-lysine methyltransferase family. EZ subfamily. Contains 1 SANT domain. Contains 1 SET domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Repressor Transferase
Gene Ontology (GO)
Biological process	regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 895	895	Histone-lysine N-methyltransferase EZ3		PRO_0000214000
Regions
	Domain	528 – 578	51	SANT
	Domain	746 – 866	121	SET
	Compositional bias	75 – 78	4	Poly-Ala
	Compositional bias	650 – 719	70	Cys-rich

Sequences

	Sequence		Length	Mass (Da)
	Q8S4P4-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 2659DCF992A08919	FASTA	895	100,393
	10 20 30 40 50 60 MASSSKASDS SSQRSKRSDQ GTGREAAPAS VVPIHGNLTQ LIRQIKSRRL LYIKEKLEAN 70 80 90 100 110 120 RKTLQRHSCS LFDVAAAAEV ASRGSDGGNA LSQRAAEGQF RLAGSDLAHG IGERDVVYMQ 130 140 150 160 170 180 EENLASGTLV LSSSGAAAQR TVVRFVKLPL VERIPPYTTW IFLDKNQRMA DDQSVVGRRR 190 200 210 220 230 240 IYYDPVGNEA LICSDSDEEI PEPEEEKHFF TEGEDQLIWR ATQEHGLNRE VVNVLCQFID 250 260 270 280 290 300 STPSEIEERS EVLFEKNEKN SGSSDKIERQ LSLDKTMDAV LDSFDNLFCR RCLVFDCRLH 310 320 330 340 350 360 GCSQNLVFPT EKQPYSFEPD ENKKPCGRQC YLRWRGGFQE IHDVGLSGCA TYNMESGTVS 370 380 390 400 410 420 HKVDVSIMSE SEDSNREKGN IRSMTLVGTS GSKIISSVSA EESTTPPSAD TSETENASSD 430 440 450 460 470 480 MPPSSLRKYK ISKRGPRYRE RSPGKRQKVF TSDISFASNI LNKLSIPEIR DTRLESREPG 490 500 510 520 530 540 GDKLQILDES TKKTSSKDIC GESPITTTEN MGIESKKVSS TKNFLEHTLS CWSALERDLY 550 560 570 580 590 600 LKGIEIFGKN SCLIARNLLS GMKTCMEVAN YMYNNGAAMA KRPLLNKSIS GDFAETEQDY 610 620 630 640 650 660 MEQDMVARTR IYRRRGRNRK LKYTWKSAGH PTVRKRIGDG KQWYTQYNPC VCQQMCGKDC 670 680 690 700 710 720 PCVENGTCCE KYCGCSKSCK NKFRGCHCAK SQCRSRQCPC FAASRECDPD VCRNCWVSCG 730 740 750 760 770 780 DGSLGEPPAR GDGYQCGNMK LLLKQQQRIL LGRSDVAGWG AFIKNPVNKN DYLGEYTGEL 790 800 810 820 830 840 ISHKEADKRG KIYDRANSSF LFDLNDQYVL DAYRKGDKLK FANHSSNPNC YAKVMLVAGD 850 860 870 880 890 HRVGIYAKEH IEASEELFYD YRYGPDQAPA WARRPEGSKK DEASVSHHRA HKVAR

References

[1]

"Sequence relationships, conserved domains, and expression patterns for maize homologs of the Polycomb group genes E(z), esc, and E(Pc)." Springer N.M., Danilevskaya O.N., Hermon P., Helentjaris T.G., Phillips R.L., Kaeppler H.F., Kaeppler S.M. Plant Physiol. 128:1332-1345(2002) [PubMed: 11950982] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Seed.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF443598 mRNA. Translation: AAM13422.1.
RefSeq	NP_001105079.1.
UniGene	Zm.10341
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	541955.
Organism-specific databases
Gramene	Q8S4P4.
MaizeGDB	754846.
Family and domain databases
InterPro	IPR001005. SANT_DNA-bd. IPR001214. SET. [Graphical view]
Pfam	PF00856. SET. 1 hit. [Graphical view]
SMART	SM00717. SANT. 1 hit. SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS51293. SANT. False negative. PS50280. SET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EZ3_MAIZE

Accession

Primary (citable) accession number: Q8S4P4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	June 1, 2002
Last modified:	September 22, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)