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Reviewed, UniProtKB/Swiss-Prot Q8S3J3 (HIUH_SOYBN)

Last modified November 24, 2009. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Hydroxyisourate hydrolase
      Short name=HIU hydrolase
      Short name=HIUHase
    EC=3.5.2.17
Gene names
Name: HIUH
OrganismGlycine max (Soybean)
Taxonomic identifier3847 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

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Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Involved in the conversion of hydroxyisourate to ureides such as allantoin, the major form of nitrogen transport in legumes. Ref.1

Catalytic activity

5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate.

Pathway

Purine metabolism; urate degradation; (S)-allantoin from urate: step 2/3.

Subunit structure

Monomer. Ref.2

Subcellular location

Peroxisome membrane; Single-pass type I membrane protein.

Tissue specificity

Highly expressed in uninfected root nodules. Detected in leaves, stems and roots. Ref.1

Developmental stage

Expression in nodules increased with increasing plant age up to 21 days, then decreased.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Biophysicochemical properties

Kinetic parameters:

KM=15 µM for hydroxyisourate

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 560529Hydroxyisourate hydrolase
PRO_0000380689

Regions

Topological domain32 – 517486Peroxisomal Potential
Transmembrane518 – 53821 Potential
Topological domain539 – 56022Cytoplasmic Potential

Sites

Active site1991Proton donor Probable
Active site4081Nucleophile Probable

Amino acid modifications

Glycosylation341N-linked (GlcNAc...) Potential
Glycosylation2261N-linked (GlcNAc...) Potential
Glycosylation2311N-linked (GlcNAc...) Potential
Glycosylation3471N-linked (GlcNAc...) Potential
Glycosylation4161N-linked (GlcNAc...) Potential
Glycosylation4891N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis1991E → A: Loss of function. Ref.1
Mutagenesis4081E → A: Loss of function. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q8S3J3-1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: CFCD946245D9E528

FASTA56063,767
        10         20         30         40         50         60 
MMEPPQTRLM INVFIVSFLA LLVNLVVGVL GADNYSRDDF PLDFVFGSGT SAYQVEGAAN 

        70         80         90        100        110        120 
KDGRTPSIWD TFAYAGYAHG ENGDVACDGY HKYKEDVQLM LETGLDAYRF SISWSRLLPN 

       130        140        150        160        170        180 
GRGPVNPKGL QYSNNLINEL ISNGIQPHAT LYNFDLPQVL EDEYGGWISR DIIRDFTYYA 

       190        200        210        220        230        240 
EVEFREFGDR VLYWTTVNEP NVFALGGYDQ GNSPPRRCSP PFCATNDTMG NSTYEPYLAV 

       250        260        270        280        290        300 
HHILLSHSSA ARLYWRKYRD KQHGFVGISI YTFGIFPQTN TEKDRVASQR ARDFFVGWIM 

       310        320        330        340        350        360 
EPLQYGDYPI SMKTNAGERI PAFTNHESKQ VKGSFDFIGV IHYTNLNVSD NSDALKNQLR 

       370        380        390        400        410        420 
DFTADMAANI FGEDLFSNEE YLITPWGLRQ ELNKFKLLYG NPPIFIHENG QRTASNSSLQ 

       430        440        450        460        470        480 
DVDKGEILHG YIGSVLDALR DASNIKGYFR MAFPGFVRVA RWIQVSFGLY YVDRDDPQLK 

       490        500        510        520        530        540 
KIPKLFCKNG TTGFLKGRRT SILDLFELEQ DPITCSKSPI IFSKISKWVL ASLLFLIQHK 

       550        560 
IKFMWREPLP GQIPLKLVMF

« Hide

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References

[1]"Cloning and expression of the gene for soybean hydroxyisourate hydrolase. Localization and implications for function and mechanism."
Raychaudhuri A., Tipton P.A.
Plant Physiol. 130:2061-2068(2002) [PubMed: 12481089] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF GLU-199 AND GLU-408, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"Identification and purification of hydroxyisourate hydrolase, a novel ureide-metabolizing enzyme."
Sarma A.D., Serfozo P., Kahn K., Tipton P.A.
J. Biol. Chem. 274:33863-33865(1999) [PubMed: 10567345] [Abstract]
Cited for: PROTEIN SEQUENCE OF 32-47, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.

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Cross-references

Sequence databases

AF486839 mRNA. Translation: AAL92115.1.
UniGeneGma.20047

3D structure databases

HSSPHSSP built from PDB template 2CBU based on UniProtKB Q08638.
ModBaseSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13506.
BRENDA3.5.2.17. 299.

Gene expression databases

GenevestigatorQ8S3J3.

Family and domain databases

InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR017853. Glyco_hydro_catalytic_core.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHERPTHR10353. Glyco_hydro_1. 1 hit.
PfamPF00232. Glyco_hydro_1. 2 hits.
[Graphical view]
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. False negative.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameHIUH_SOYBN
AccessionPrimary (citable) accession number: Q8S3J3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 1, 2002
Last modified: November 24, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents