Q8S341 (PPA7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Purple acid phosphatase 7
EC=3.1.3.2

Gene names

Name:	PAP7
Ordered Locus Names:	At2g01880
ORF Names:	T23K3.7

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed in roots, stems, leaves, flowers and siliques. Ref.6
Sequence similarities	Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.
Sequence caution	The sequence AAD21780.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 328	304	Purple acid phosphatase 7		PRO_0000372812
Regions
	Region	248 – 250	3	Substrate binding By similarity
Sites
	Active site	222	1	Proton donor By similarity
	Metal binding	48	1	Iron By similarity
	Metal binding	81	1	Iron By similarity
	Metal binding	81	1	Zinc By similarity
	Metal binding	84	1	Iron By similarity
	Metal binding	119	1	Zinc By similarity
	Metal binding	213	1	Zinc By similarity
	Metal binding	248	1	Zinc By similarity
	Metal binding	250	1	Iron By similarity
Amino acid modifications
	Glycosylation	56	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	317	1	H → L in BAC43423. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8S341 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: F897E0A8D39CA052	FASTA	328	37,549
	10 20 30 40 50 60 MKMHVCFSVI LMFLSIFFIN GALSKLERLK HPVKKKSDGS LSFLVIGDWG RKGGFNQSLV 70 80 90 100 110 120 AHQMGVVGEK LDIDFVISVG DNFYDDGLKG VNDPSFEASF SHIYTHPSLQ KQWYSVLGNH 130 140 150 160 170 180 DYRGNVEAQL SKVLTQKDWR WFCRRSFVLS SGMVDFFFAD TNPFVEKYFT EPEDHTYDWR 190 200 210 220 230 240 NVLPRNKYIS NLLHDLDLEI KKSRATWKFV VGHHGIKTAG NHGVTQELVD QLLPILEENK 250 260 270 280 290 300 VDLYINGHDH CLQHIGSHGK TQFLTSGGGS KAWRGHVQPW DPKELKLYYD GQGFMSLHIT 310 320 HSKAKFIYYD VSGNVLHRSS LSKRSAHL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation." Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D. J. Biol. Chem. 277:27772-27781(2002) [PubMed: 12021284] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE. Strain: cv. Col-1.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Arabidopsis ORF Clones." Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower." Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D. Plant Mol. Biol. 59:581-594(2005) [PubMed: 16244908] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF492659 mRNA. Translation: AAM15908.1. AC007069 Genomic DNA. Translation: AAD21780.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC05511.1. AK118834 mRNA. Translation: BAC43423.1. BT026449 mRNA. Translation: ABH04556.1.
IPI	IPI00530571.
PIR	C84430.
RefSeq	NP_178297.2. NM_126249.4.
UniGene	At.42460.
3D structure databases
HSSP	HSSP built from PDB template 1QFC based on UniProtKB P29288.
ProteinModelPortal	Q8S341.
SMR	Q8S341. Positions 38-326.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8S341.
Proteomic databases
PRIDE	Q8S341.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G01880.1; AT2G01880.1; AT2G01880.
GeneID	814719.
GenomeReviews	Gene locus AT2G01880 in contig CT485783_GR.
KEGG	ath:AT2G01880.
NMPDR	fig|3702.1.peg.7792.
Organism-specific databases
TAIR	At2g01880.
Phylogenomic databases
eggNOG	KOG2679.
GeneTree	EPGT00050000012616.
InParanoid	Q8S341.
OMA	WHGNAHA.
PhylomeDB	Q8S341.
ProtClustDB	CLSN2688499.
Gene expression databases
Genevestigator	Q8S341.
Family and domain databases
InterPro	IPR024927. Acid_Pase_5. IPR004843. Metallo_PEstase_dom. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF000898. Acid_Ptase_5. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPA7_ARATH

Accession

Primary (citable) accession number: Q8S341
Secondary accession number(s): Q8GWH8, Q9SIS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program