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Protein

Purple acid phosphatase 22

Gene

PAP22

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi148IronBy similarity1
Metal bindingi175IronBy similarity1
Metal bindingi175ZincBy similarity1
Metal bindingi178IronBy similarity1
Metal bindingi208ZincBy similarity1
Binding sitei208SubstrateBy similarity1
Metal bindingi292ZincBy similarity1
Active sitei302Proton donorBy similarity1
Metal bindingi329ZincBy similarity1
Metal bindingi331IronBy similarity1

GO - Molecular functioni

  • acid phosphatase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase
LigandIron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G52820-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 22 (EC:3.1.3.2)
Gene namesi
Name:PAP22
Synonyms:AT6
Ordered Locus Names:At3g52820
ORF Names:F3C22.220
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G52820
TAIRilocus:2083238 AT3G52820

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000037282523 – 434Purple acid phosphatase 22Add BLAST412

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi116N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi403N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8S340

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ8S340 baseline and differential
GenevisibleiQ8S340 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G52820.1

Structurei

3D structure databases

ProteinModelPortaliQ8S340
SMRiQ8S340
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni329 – 331Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1378 Eukaryota
COG1409 LUCA
HOGENOMiHOG000238330
InParanoidiQ8S340
KOiK22390
OMAiNGPEFSF
OrthoDBiEOG09360ARG
PhylomeDBiQ8S340

Family and domain databases

Gene3Di2.60.40.380, 1 hit
3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR008963 Purple_acid_Pase-like_N
IPR015914 Purple_acid_Pase_N
IPR025733 Purple_acid_PPase_C_dom
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PF14008 Metallophos_C, 1 hit
PF16656 Pur_ac_phosph_N, 1 hit
SUPFAMiSSF49363 SSF49363, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8S340-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLFGLFLSF TLLFLCPFIS QADVPELSRQ PPRPIVFVHN DRSKSDPQQV
60 70 80 90 100
HISLAGKDHM RVTFITEDNK VESVVEYGKQ PGKYDGKATG ECTSYKYFFY
110 120 130 140 150
KSGKIHHVKI GPLQANTTYY YRCGGNGPEF SFKTPPSTFP VEFAIVGDLG
160 170 180 190 200
QTEWTAATLS HINSQDYDVF LLPGDLSYAD THQPLWDSFG RLVEPLASKR
210 220 230 240 250
PWMVTEGNHE IEFFPIIEHT TFKSYNARWL MPHTESFSTS NLYYSFDVAG
260 270 280 290 300
VHTVMLGSYT DFDCESDQYQ WLQADLAKVD RKTTPWVVVL LHAPWYNTNE
310 320 330 340 350
AHEGEGESMR EAMESLLFNA RVDVVFSGHV HAYERFKRVY NNKADPCGPI
360 370 380 390 400
HITIGDGGNR EGLALSFKKP PSPLSEFRES SFGHGRLKVM DGKRAHWSWH
410 420 430
RNNDSNSLLA DEVWLDSLST SSSCWPSSRS NDEL
Length:434
Mass (Da):49,357
Last modified:June 1, 2002 - v1
Checksum:i103381F91CB4EB00
GO

Sequence cautioni

The sequence CAB89243 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492668 mRNA Translation: AAM15917.1
AL353912 Genomic DNA Translation: CAB89243.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE78997.1
AK118546 mRNA Translation: BAC43148.1
BT006218 mRNA Translation: AAP12867.1
PIRiT49035
RefSeqiNP_190850.2, NM_115142.5
UniGeneiAt.35258

Genome annotation databases

EnsemblPlantsiAT3G52820.1; AT3G52820.1; AT3G52820
GeneIDi824448
GrameneiAT3G52820.1; AT3G52820.1; AT3G52820
KEGGiath:AT3G52820

Similar proteinsi

Entry informationi

Entry nameiPPA22_ARATH
AccessioniPrimary (citable) accession number: Q8S340
Secondary accession number(s): Q8GWZ2, Q9LXI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: June 1, 2002
Last modified: April 25, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health