Reviewed, UniProtKB/Swiss-Prot Q8S340 (PPA22_ARATH)

Names and origin

Protein names

Recommended name:
    Purple acid phosphatase 22
    EC=3.1.3.2

Gene names

Name:	PAP22
Synonyms:	AT6
Ordered Locus Names:	At3g52820
ORF Names:	F3C22.220

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed in roots, stems, leaves, flowers and siliques. Ref.5
Sequence similarities	Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.
Sequence caution	The sequence CAB89243.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 434	412	Purple acid phosphatase 22		PRO_0000372825
Regions
	Region	329 – 331	3	Substrate binding By similarity
Sites
	Active site	302	1	Proton donor By similarity
	Metal binding	148	1	Iron By similarity
	Metal binding	175	1	Iron By similarity
	Metal binding	175	1	Zinc By similarity
	Metal binding	178	1	Iron By similarity
	Metal binding	208	1	Zinc By similarity
	Metal binding	292	1	Zinc By similarity
	Metal binding	329	1	Zinc By similarity
	Metal binding	331	1	Iron By similarity
	Binding site	208	1	Substrate By similarity
Amino acid modifications
	Glycosylation	116	1	N-linked (GlcNAc...) Potential
	Glycosylation	403	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8S340-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 103381F91CB4EB00	FASTA	434	49,357
	10 20 30 40 50 60 MKLFGLFLSF TLLFLCPFIS QADVPELSRQ PPRPIVFVHN DRSKSDPQQV HISLAGKDHM 70 80 90 100 110 120 RVTFITEDNK VESVVEYGKQ PGKYDGKATG ECTSYKYFFY KSGKIHHVKI GPLQANTTYY 130 140 150 160 170 180 YRCGGNGPEF SFKTPPSTFP VEFAIVGDLG QTEWTAATLS HINSQDYDVF LLPGDLSYAD 190 200 210 220 230 240 THQPLWDSFG RLVEPLASKR PWMVTEGNHE IEFFPIIEHT TFKSYNARWL MPHTESFSTS 250 260 270 280 290 300 NLYYSFDVAG VHTVMLGSYT DFDCESDQYQ WLQADLAKVD RKTTPWVVVL LHAPWYNTNE 310 320 330 340 350 360 AHEGEGESMR EAMESLLFNA RVDVVFSGHV HAYERFKRVY NNKADPCGPI HITIGDGGNR 370 380 390 400 410 420 EGLALSFKKP PSPLSEFRES SFGHGRLKVM DGKRAHWSWH RNNDSNSLLA DEVWLDSLST 430 SSSCWPSSRS NDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation." Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D. J. Biol. Chem. 277:27772-27781(2002) [PubMed: 12021284] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE. Strain: cv. Col-1.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 172-434. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 203-434. Strain: cv. Columbia.
[5]	"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower." Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D. Plant Mol. Biol. 59:581-594(2005) [PubMed: 16244908] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF492668 mRNA. Translation: AAM15917.1. AL353912 Genomic DNA. Translation: CAB89243.1. Sequence problems. AK118546 mRNA. Translation: BAC43148.1. BT006218 mRNA. Translation: AAP12867.1.
IPI	IPI00541443.
PIR	T49035.
RefSeq	NP_190850.2.
UniGene	At.35258
3D structure databases
HSSP	HSSP built from PDB template 4KBP based on UniProtKB P80366.
SMR	Q8S340. Positions 45-416.
ModBase	Search...
Proteomic databases
PRIDE	Q8S340.
Genome annotation databases
GeneID	824448.
GenomeReviews	Gene locus AT3G52820 in contig BA000014_GR.
KEGG	ath:AT3G52820.
NMPDR	fig|3702.1.peg.16554.
Organism-specific databases
TAIR	At3g52820.
Phylogenomic databases
eggNOG	KOG1378.
InParanoid	Q8S340.
OMA	LTRSHDN.
PhylomeDB	Q8S340.
Gene expression databases
Genevestigator	Q8S340.
Family and domain databases
InterPro	IPR004843. M-pesterase. IPR008963. Purple_acid_Pase-like_N. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPA22_ARATH

Accession

Primary (citable) accession number: Q8S340
Secondary accession number(s): Q8GWZ2, Q9LXI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	June 1, 2002
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)