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Protein

Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 2, cytosolic

Gene

Os01g0801600

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May be involved in cell redox homeostasis.By similarity

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei195NAD; via amide nitrogenBy similarity1
Binding sitei195SubstrateBy similarity1
Binding sitei234NAD; via amide nitrogenBy similarity1
Active sitei284Proton acceptorBy similarity1
Binding sitei346NADBy similarity1
Binding sitei374NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi47 – 52NADBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 2, cytosolic (EC:1.1.1.8)
Gene namesi
Ordered Locus Names:Os01g0801600, LOC_Os01g58740
ORF Names:OsJ_03778, P0003D09.23
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004201781 – 467Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 2, cytosolicAdd BLAST467

Proteomic databases

PaxDbiQ8S2G5
PRIDEiQ8S2G5

Expressioni

Gene expression databases

ExpressionAtlasiQ8S2G5 baseline and differential
GenevisibleiQ8S2G5 OS

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os01g58740.1

Structurei

3D structure databases

ProteinModelPortaliQ8S2G5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni346 – 347Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2711 Eukaryota
COG0240 LUCA
InParanoidiQ8S2G5
KOiK00006
OMAiHPRIVTP
OrthoDBiEOG093609YK

Family and domain databases

Gene3Di1.10.1040.10, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR013328 6PGD_dom2
IPR006168 G3P_DH_NAD-dep
IPR006109 G3P_DH_NAD-dep_C
IPR011128 G3P_DH_NAD-dep_N
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF07479 NAD_Gly3P_dh_C, 1 hit
PF01210 NAD_Gly3P_dh_N, 1 hit
PRINTSiPR00077 GPDHDRGNASE
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 2 hits

Sequencei

Sequence statusi: Complete.

Q8S2G5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGAEDAPRA AAANGHGNGA TVEEKLDELR RLLGKADGDP LRIVGVGAGA
60 70 80 90 100
WGSVFCALMQ DAYGHLRDKV QVRIWRRPGR AVDRATAEHL FEVINAREDV
110 120 130 140 150
LRRLIRRCAY LKYVEGRLGD RTLYADEILR DGFCLNMIDT PLCPLKVVTN
160 170 180 190 200
LQEAVWDADI VINGLPSTDT REVFGEIGRY WKERITAPII LSLAKGIEAS
210 220 230 240 250
LDPLPRIITP TQMISNATGV PLENILYLGG PNIASEIYNK EYANARICGA
260 270 280 290 300
DKWRKPLAKF LRQPHFIVWD NSDLITHEVM GGLKNVYAIG AGMVAALTNE
310 320 330 340 350
SATSKSVYFA LCTSEMIYIT HLLEEEPEKL AGPLLADTYV TLLKGRNAWY
360 370 380 390 400
GQKLAKGELT LEMGDSIKGK GTIQGVSAVN AFYELLSQDS LSVMHPEANR
410 420 430 440 450
SVAPVEMCPI LKALYKILIK RELPPDSILQ AIRDETMYDP RERIEMAQGH
460
SLYRPSLLGQ PKGDAKA
Length:467
Mass (Da):51,583
Last modified:June 1, 2002 - v1
Checksum:i7857AB03C7E6B051
GO

Sequence cautioni

The sequence AAO72676 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti163 – 165NGL → KRV in AAO72676 (PubMed:14750518).Curated3
Sequence conflicti394M → V in AK073318 (PubMed:12869764).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003221 Genomic DNA Translation: BAB86423.1
AP008207 Genomic DNA Translation: BAF06455.1
AP014957 Genomic DNA Translation: BAS74801.1
CM000138 Genomic DNA Translation: EAZ13855.1
AK073318 mRNA No translation available.
AY224556 mRNA Translation: AAO72676.1 Different initiation.
RefSeqiXP_015617993.1, XM_015762507.1
UniGeneiOs.88558

Genome annotation databases

EnsemblPlantsiOs01t0801600-01; Os01t0801600-01; Os01g0801600
GeneIDi4327964
GrameneiOs01t0801600-01; Os01t0801600-01; Os01g0801600
KEGGiosa:4327964

Similar proteinsi

Entry informationi

Entry nameiGPDH2_ORYSJ
AccessioniPrimary (citable) accession number: Q8S2G5
Secondary accession number(s): A0A0N7KDX1, Q84VD0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health