Q8S2E5 (KPRS3_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase 3, chloroplastic
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase 3

Gene names

Ordered Locus Names:	Os01g0723600, LOC_Os01g52530
ORF Names:	P0022F10.17

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 44	44	Chloroplast Potential
	Chain	45 – 409	365	Ribose-phosphate pyrophosphokinase 3, chloroplastic		PRO_0000141099
Regions
	Region	312 – 327	16	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	229	1	Magnesium By similarity
	Metal binding	231	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8S2E5 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 1C801F4D86AFF9A1	FASTA	409	43,909
	10 20 30 40 50 60 MATAASASAS ASPAAAFGAK TRRPGPSPSP SPSPASAFAR PSPRASAAGR LHASLHLGGA 70 80 90 100 110 120 SATGSSIVSN ASGIHLAAPV LAPLAVPKMT GAVGAHKNVL LFHCEEMREL AEQVVARNDD 130 140 150 160 170 180 IELRSISWRT FADGFPNLFI SNAHTIRGQH VAFLASFSSP SVIFEQLSII YALPKLFISS 190 200 210 220 230 240 FTLILPFFPT GTSERMEDEG DVATAFTLAR ILSNIPISRG GPSSLVIFDI HALQERFYFG 250 260 270 280 290 300 DSVLPCFESG IPLLKSRLQE LPDSDNITIA FPDDGAWKRF YKQLQHFPMV VCNKVREGEQ 310 320 330 340 350 360 RIVRIKEGDP RGRHVVIVDD LVQSGGTLIE CQKVLAEHGA AKVSAYVTHG IFPNKSWEKF 370 380 390 400 QPDNGEGPGH GLSHFWITDS CPLTVNAVKD RQPFEILSLA GPIASALQI

References

[1]

"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

[2]

"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003229 Genomic DNA. Translation: BAB89498.1. AK109735 mRNA. No translation available.
RefSeq	NP_001044108.1. NM_001050643.1.
UniGene	Os.29866.
3D structure databases
ProteinModelPortal	Q8S2E5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8S2E5.
Proteomic databases
PRIDE	Q8S2E5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os01g52530.1; LOC_Os01g52530.1; LOC_Os01g52530.
GeneID	4324718.
KEGG	osa:4324718.
NMPDR	fig|39947.1.peg.8781.
Organism-specific databases
Gramene	Q8S2E5.
Phylogenomic databases
GeneTree	EPGT00070000029448.
OMA	TSERMED.
PhylomeDB	Q8S2E5.
ProtClustDB	PLN02297.
Family and domain databases
InterPro	IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS3_ORYSJ

Accession

Primary (citable) accession number: Q8S2E5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program