Reviewed, UniProtKB/Swiss-Prot Q8RYM9 (LAC2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Laccase-2
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 2
    Urishiol oxidase 2
    Diphenol oxidase 2

Gene names

Name:	LAC2
Ordered Locus Names:	Os01g0634500, LOC_Os01g44330
ORF Names:	P0663E10.27, OsJ_002637

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Apoplast Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 562	536	Laccase-2		PRO_0000291887
Regions
	Domain	34 – 150	117	Plastocyanin-like 1
	Domain	160 – 312	153	Plastocyanin-like 2
	Domain	411 – 546	136	Plastocyanin-like 3
Sites
	Metal binding	84	1	Copper 1 By similarity
	Metal binding	86	1	Copper 2 By similarity
	Metal binding	129	1	Copper 2 By similarity
	Metal binding	131	1	Copper 3 By similarity
	Metal binding	463	1	Copper 4 By similarity
	Metal binding	466	1	Copper 1 By similarity
	Metal binding	468	1	Copper 3 By similarity
	Metal binding	525	1	Copper 3 By similarity
	Metal binding	526	1	Copper 4 By similarity
	Metal binding	527	1	Copper 2 By similarity
	Metal binding	531	1	Copper 4 By similarity
Amino acid modifications
	Glycosylation	39	1	N-linked (GlcNAc...) Potential
	Glycosylation	53	1	N-linked (GlcNAc...) Potential
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
	Glycosylation	80	1	N-linked (GlcNAc...) Potential
	Glycosylation	118	1	N-linked (GlcNAc...) Potential
	Glycosylation	189	1	N-linked (GlcNAc...) Potential
	Glycosylation	244	1	N-linked (GlcNAc...) Potential
	Glycosylation	300	1	N-linked (GlcNAc...) Potential
	Glycosylation	328	1	N-linked (GlcNAc...) Potential
	Glycosylation	376	1	N-linked (GlcNAc...) Potential
	Glycosylation	386	1	N-linked (GlcNAc...) Potential
	Glycosylation	421	1	N-linked (GlcNAc...) Potential
	Glycosylation	445	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	168	1	E → D in EAZ12812. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8RYM9-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 0BF4F84B9E7996A9	FASTA	562	61,296
	10 20 30 40 50 60 MASAASSLPL LVSSLLLALF ALGAHADVKR YQFDIVMSNV SRLCHEKAMV TVNGSYPGPT 70 80 90 100 110 120 IYAREGDRVI VNVTNHVKHN MTIHWHGLKQ RRNGWADGPA YVTQCPIGSG GSYVYDFNVT 130 140 150 160 170 180 RQRGTLWWHA HIAWMRATVH GAIVILPAAG VPYPFPKPDD EAEIVLGEWW HADVETVERQ 190 200 210 220 230 240 GSMLGMAPNM SDAHTINGKP GPLVPFCSEK HTYALQVQSG KTYLLRIINA AVNDELFFSI 250 260 270 280 290 300 AGHNMTVVEI DATYTKPFAA STVQLSPGQT MNVLVSADQS PGRYFMVAKP FNDVPIPADN 310 320 330 340 350 360 KTATAILQYA GVPTSVVPAL PQTMPATNST GSVAAFHDKL RSLNSPRYPA DVPLAVDRHL 370 380 390 400 410 420 LYTIGLNIDP CETCLNRSRL AASLNNITFV MPRTALLQAH YYGQKGVFAA DFPDRPPARF 430 440 450 460 470 480 NYTGVPLTAG LGTSLGTRLS KIAYNATVEL VLQDTNLLSV ESHPFHLHGY NFFVVGRGVG 490 500 510 520 530 540 NFDPAKDPAK YNLVDPPERN TVGVPAGGWT AIRFRADNPG VWFLHCHLEV HTSWGLKMAF 550 560 LVEDGSGPDE SVLPPPKDLP KC

References

[1]

"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

[2]

"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004317 Genomic DNA. Translation: BAB90733.1. CM000138 Genomic DNA. Translation: EAZ12812.1.
3D structure databases
HSSP	HSSP built from PDB template 1GYC based on UniProtKB Q12718.
ModBase	Search...
Genome annotation databases
NMPDR	fig|39947.1.peg.9914.
Organism-specific databases
Gramene	Q8RYM9.
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. IPR017761. Laccase. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR03389. laccase. 1 hit.
PROSITE	PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC2_ORYSJ

Accession

Primary (citable) accession number: Q8RYM9
Secondary accession number(s): A2ZVR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	June 1, 2002
Last modified:	February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)