Q8RYC8 (ARFS_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Auxin response factor 19

Alternative name(s):
Auxin-responsive protein IAA22

Gene names

Name:	ARF19
Synonyms:	IAA22
Ordered Locus Names:	At1g19220
ORF Names:	T29M8.9

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1086 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Auxin response factors (ARFs) are transcriptional factors that binds specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Could act as transcriptional activator or repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Involved in ethylene responses. Regulates lateral root formation through direct regulation of LBD16 and/or LBD29. Functionally redundant with ARF7. Ref.8 Ref.9 Ref.10
Subunit structure	Homo- and heterodimers. Interacts with the auxin-responsive protein IAA1. Ref.1
Subcellular location	Nucleus Ref.1.
Induction	By auxin and ethylene. Ref.9
Domain	Interactions between auxin response factors (ARFs) and Aux/IAA proteins occur through their C-terminal dimerization domains III and IV. Ref.1
Sequence similarities	Belongs to the ARF family. Contains 1 Aux/IAA-ARF domain. Contains 1 TF-B3 DNA-binding domain.
Sequence caution	The sequence AAF82232.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Auxin signaling pathway Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	auxin mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW lateral root primordium development Inferred from genetic interaction Ref.10. Source: TAIR leaf development Inferred from genetic interaction. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to ethylene stimulus Inferred from mutant phenotype Ref.9. Source: TAIR transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Traceable author statement Ref.1. Source: UniProtKB
Molecular function	DNA binding Inferred from direct assay Ref.10. Source: TAIR protein dimerization activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1086	1086	Auxin response factor 19		PRO_0000111523
Regions
	Domain	959 – 1041	83	Aux/IAA-ARF
	DNA binding	126 – 228	103	TF-B3
	Compositional bias	483 – 724	242	Gln-rich

Sequences

	Sequence		Length	Mass (Da)
	Q8RYC8 [UniParc]. Last modified April 26, 2004. Version 2. Checksum: 202C0944581E0545	FASTA	1,086	120,576
	10 20 30 40 50 60 MKAPSNGFLP SSNEGEKKPI NSQLWHACAG PLVSLPPVGS LVVYFPQGHS EQVAASMQKQ 70 80 90 100 110 120 TDFIPNYPNL PSKLICLLHS VTLHADTETD EVYAQMTLQP VNKYDREALL ASDMGLKLNR 130 140 150 160 170 180 QPTEFFCKTL TASDTSTHGG FSVPRRAAEK IFPPLDFSMQ PPAQEIVAKD LHDTTWTFRH 190 200 210 220 230 240 IYRGQPKRHL LTTGWSVFVS TKRLFAGDSV LFVRDEKSQL MLGIRRANRQ TPTLSSSVIS 250 260 270 280 290 300 SDSMHIGILA AAAHANANSS PFTIFFNPRA SPSEFVVPLA KYNKALYAQV SLGMRFRMMF 310 320 330 340 350 360 ETEDCGVRRY MGTVTGISDL DPVRWKGSQW RNLQVGWDES TAGDRPSRVS IWEIEPVITP 370 380 390 400 410 420 FYICPPPFFR PKYPRQPGMP DDELDMENAF KRAMPWMGED FGMKDAQSSM FPGLSLVQWM 430 440 450 460 470 480 SMQQNNPLSG SATPQLPSAL SSFNLPNNFA SNDPSKLLNF QSPNLSSANS QFNKPNTVNH 490 500 510 520 530 540 ISQQMQAQPA MVKSQQQQQQ QQQQHQHQQQ QLQQQQQLQM SQQQVQQQGI YNNGTIAVAN 550 560 570 580 590 600 QVSCQSPNQP TGFSQSQLQQ QSMLPTGAKM THQNINSMGN KGLSQMTSFA QEMQFQQQLE 610 620 630 640 650 660 MHNSSQLLRN QQEQSSLHSL QQNLSQNPQQ LQMQQQSSKP SPSQQLQLQL LQKLQQQQQQ 670 680 690 700 710 720 QSIPPVSSSL QPQLSALQQT QSHQLQQLLS SQNQQPLAHG NNSFPASTFM QPPQIQVSPQ 730 740 750 760 770 780 QQGQMSNKNL VAAGRSHSGH TDGEAPSCST SPSANNTGHD NVSPTNFLSR NQQQGQAASV 790 800 810 820 830 840 SASDSVFERA SNPVQELYTK TESRISQGMM NMKSAGEHFR FKSAVTDQID VSTAGTTYCP 850 860 870 880 890 900 DVVGPVQQQQ TFPLPSFGFD GDCQSHHPRN NLAFPGNLEA VTSDPLYSQK DFQNLVPNYG 910 920 930 940 950 960 NTPRDIETEL SSAAISSQSF GIPSIPFKPG CSNEVGGIND SGIMNGGGLW PNQTQRMRTY 970 980 990 1000 1010 1020 TKVQKRGSVG RSIDVTRYSG YDELRHDLAR MFGIEGQLED PLTSDWKLVY TDHENDILLV 1030 1040 1050 1060 1070 1080 GDDPWEEFVN CVQNIKILSS VEVQQMSLDG DLAAIPTTNQ ACSETDSGNA WKVHYEDTSA AASFNR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Protein-protein interactions among the Aux/IAA proteins." Kim J., Harter K., Theologis A. Proc. Natl. Acad. Sci. U.S.A. 94:11786-11791(1997) [PubMed: 9342315] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, SUBCELLULAR LOCATION, DOMAIN. Strain: cv. Columbia. Tissue: Etiolated seedling.
[2]	Arima K., Overvoorde P.J., Theologis A. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana: unique and overlapping functions of ARF7 and ARF19." Okushima Y., Overvoorde P.J., Arima K., Alonso J.M., Chan A., Chang C., Ecker J.R., Hughes B., Lui A., Nguyen D., Onodera C., Quach H., Smith A., Yu G., Theologis A. Plant Cell 17:444-463(2005) [PubMed: 15659631] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Columbia.
[4]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[6]	"ORF cloning and analysis of Arabidopsis transcription factor genes." Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y., Takagi M. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Nucleotide sequence of the Arabidopsis IAA22." Ciarbelli A.R., Carabelli M., Ruzza V., Sessa G., Steindler C., Ruberti I. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 809-1086. Strain: cv. Columbia.
[8]	"Auxin-responsive gene expression: genes, promoters and regulatory factors." Hagen G., Guilfoyle T.J. Plant Mol. Biol. 49:373-385(2002) [PubMed: 12036261] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
[9]	"A role for auxin response factor 19 in auxin and ethylene signaling in Arabidopsis." Li J., Dai X., Zhao Y. Plant Physiol. 140:899-908(2006) [PubMed: 16461383] [Abstract] Cited for: FUNCTION, INDUCTION.
[10]	"ARF7 and ARF19 regulate lateral root formation via direct activation of LBD/ASL genes in Arabidopsis." Okushima Y., Fukaki H., Onoda M., Theologis A., Tasaka M. Plant Cell 19:118-130(2007) [PubMed: 17259263] [Abstract] Cited for: FUNCTION.
[11]	"The plant B3 superfamily." Swaminathan K., Peterson K., Jack T. Trends Plant Sci. 13:647-655(2008) [PubMed: 18986826] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U53672 mRNA. Translation: AAB91321.2. AY008390 Genomic DNA. Translation: AAG35176.1. AY669794 mRNA. Translation: AAT67078.1. AC069143 Genomic DNA. Translation: AAF82232.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE29819.1. AB493467 mRNA. Translation: BAH30305.1. AJ441312 mRNA. Translation: CAD29695.1.
IPI	IPI00534870.
PIR	G86325.
RefSeq	NP_173356.1. NM_101780.3.
UniGene	At.10488.
3D structure databases
ProteinModelPortal	Q8RYC8.
SMR	Q8RYC8. Positions 120-230.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8RYC8. 25 interactions.
STRING	Q8RYC8.
Proteomic databases
PRIDE	Q8RYC8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G19220.1; AT1G19220.1; AT1G19220.
GeneID	838505.
GenomeReviews	Gene locus AT1G19220 in contig CT485782_GR.
KEGG	ath:AT1G19220.
NMPDR	fig|3702.1.peg.2265.
Organism-specific databases
GeneFarm	3421. 345.
TAIR	At1g19220.
Phylogenomic databases
GeneTree	EPGT00070000028440.
HOGENOM	HBG746399.
InParanoid	Q8RYC8.
OMA	WEIEPVI.
PhylomeDB	Q8RYC8.
ProtClustDB	CLSN2914206.
Gene expression databases
ArrayExpress	Q8RYC8.
Genevestigator	Q8RYC8.
GermOnline	AT1G19220. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003311. AUX_IAA. IPR011525. AuxIAA_ARF_dimer. IPR010525. Auxin_resp. IPR003340. B3_DNA-bd. IPR015300. DNA-bd_pseudobarrel. [Graphical view]
Gene3D	G3DSA:2.40.330.10. G3DSA:2.40.330.10. 1 hit.
Pfam	PF02309. AUX_IAA. 1 hit. PF06507. Auxin_resp. 1 hit. PF02362. B3. 1 hit. [Graphical view]
SMART	SM01019. B3. 1 hit. [Graphical view]
SUPFAM	SSF101936. Restrict_endonuc_II_EcoRII_N. 1 hit.
PROSITE	PS50863. B3. 1 hit. PS50962. IAA_ARF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARFS_ARATH

Accession

Primary (citable) accession number: Q8RYC8
Secondary accession number(s): O49961, Q5IRX3, Q9LMA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	April 26, 2004
Last modified:	January 25, 2012
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program