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Protein

Adenylosuccinate lyase

Gene

At4g18440

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
N6-(1,2-dicarboxyethyl)AMP = fumarate + AMP.UniRule annotation

Pathwayi: AMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes AMP from IMP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Adenylosuccinate synthetase, chloroplastic (PURA), Adenylosuccinate synthetase, chloroplastic (PURA), Adenylosuccinate synthetase, chloroplastic (PURA)
  2. Adenylosuccinate lyase (AXX17_At1g37010), Adenylosuccinate lyase (At1g36280), Adenylosuccinate lyase (At1g36280), Adenylosuccinate lyase (F15C21.8), Adenylosuccinate lyase (AXX17_At4g21680), Adenylosuccinate lyase (At4g18440)
This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylaminoimidazole-succinocarboxamide synthase, chloroplastic (PUR7)
  2. Adenylosuccinate lyase (AXX17_At1g37010), Adenylosuccinate lyase (At1g36280), Adenylosuccinate lyase (At1g36280), Adenylosuccinate lyase (F15C21.8), Adenylosuccinate lyase (AXX17_At4g21680), Adenylosuccinate lyase (At4g18440)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationImported
Biological processPurine biosynthesisUniRule annotation

Enzyme and pathway databases

BioCyciARA:AT4G18440-MONOMER.
ReactomeiR-ATH-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00132.
UPA00075; UER00336.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylosuccinate lyaseUniRule annotation (EC:4.3.2.2UniRule annotation)
Short name:
ASLUniRule annotation
Alternative name(s):
AdenylosuccinaseUniRule annotation
Gene namesi
Ordered Locus Names:At4g18440Imported
ORF Names:F28J12.100Imported, F28J12_100Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G18440.
TAIRilocus:2124494. AT4G18440.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

PTM databases

iPTMnetiQ8RY94.

Interactioni

Protein-protein interaction databases

IntActiQ8RY94. 1 interactor.
STRINGi3702.AT4G18440.1.

Structurei

3D structure databases

SMRiQ8RY94.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini126 – 388Lyase_1InterPro annotationAdd BLAST263
Domaini404 – 518ASL_CInterPro annotationAdd BLAST115

Sequence similaritiesi

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2700. Eukaryota.
COG0015. LUCA.
HOGENOMiHOG000252916.
KOiK01756.
OMAiCESQPEI.
OrthoDBiEOG093607L3.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
InterProiView protein in InterPro
IPR024083. Fumarase/histidase_N.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
IPR004769. Pur_lyase.
IPR013539. PurB_C.
PfamiView protein in Pfam
PF08328. ASL_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00928. purB. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8RY94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELNFSSMAI THPKIPSFGF TPTGIFLNPS KSVCLASHHR LPRVSCSVST
60 70 80 90 100
TTDSPKLVTS TKVTAMDGVS SRDLEMSNLT ALSPLDGRYW SKVKDLASSL
110 120 130 140 150
SEFGLIYFRV FVEIKWLLKL SNIPEVTEVP SFSKEAQSFL QGIIDGFSID
160 170 180 190 200
DALEIKKIER VTNHDVKAVE YFLKQKCESQ PEIAKVLEFF HFACTSEDIN
210 220 230 240 250
NLSHALMLQE ALSSVILPTM DELIKSISLI AKNFAYVPML SRTHGQPATP
260 270 280 290 300
TTLGKEMANF AVRLSEERRY LSETKIKGKF AGAVGNYNAH ISAYSNIDWP
310 320 330 340 350
HVSEEFVTSL GLTFNPYVTQ IEPHDYMARL FNNISQFNTI LIDFDRDIWS
360 370 380 390 400
YISLGYFKQT TKAGEIGSST MPHKVNPIDF ENSEGNLGKA NAELTFLSMK
410 420 430 440 450
LPISRMQRDL TDSTVLRNMG GALGHSLLAY KSAIQGIGKL QVNEARLKED
460 470 480 490 500
LDDNWEVLAE PIQTVMRRYG VPEPYEKLKE LTRGKAVNEE TIRTFIKGLE
510 520 530
LPSEAKDQLL ELTPHTYVGA AAALALAVDE ALHLGH
Length:536
Mass (Da):59,757
Last modified:June 1, 2002 - v1
Checksum:i0D2157431FF2D590
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY074500 mRNA. Translation: AAL69484.1.
AY150476 mRNA. Translation: AAN13001.1.
CP002687 Genomic DNA. Translation: AEE84046.1.
RefSeqiNP_193579.2. NM_117957.5.
UniGeneiAt.23844.

Genome annotation databases

EnsemblPlantsiAT4G18440.1; AT4G18440.1; AT4G18440.
GeneIDi827575.
GrameneiAT4G18440.1; AT4G18440.1; AT4G18440.
KEGGiath:AT4G18440.

Similar proteinsi

Entry informationi

Entry nameiQ8RY94_ARATH
AccessioniPrimary (citable) accession number: Q8RY94
Entry historyiIntegrated into UniProtKB/TrEMBL: June 1, 2002
Last sequence update: June 1, 2002
Last modified: October 25, 2017
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported