ID   PI5K8_ARATH             Reviewed;         769 AA.
AC   Q8RY89; Q9C962;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   27-MAR-2024, entry version 134.
DE   RecName: Full=Phosphatidylinositol 4-phosphate 5-kinase 8;
DE            Short=AtPIP5K8;
DE            EC=2.7.1.68;
DE   AltName: Full=1-phosphatidylinositol 4-phosphate kinase 8;
DE   AltName: Full=Diphosphoinositide kinase 8;
DE   AltName: Full=PtdIns(4)P-5-kinase 8;
GN   Name=PIP5K8; OrderedLocusNames=At1g60890; ORFNames=T7P1.4;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=12226484; DOI=10.1104/pp.004770;
RA   Mueller-Roeber B., Pical C.;
RT   "Inositol phospholipid metabolism in Arabidopsis. Characterized and
RT   putative isoforms of inositol phospholipid kinase and phosphoinositide-
RT   specific phospholipase C.";
RL   Plant Physiol. 130:22-46(2002).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19376835; DOI=10.1104/pp.109.138677;
RA   Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA   Grossmann J., Gruissem W., Baginsky S.;
RT   "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT   chloroplast kinase substrates and phosphorylation networks.";
RL   Plant Physiol. 150:889-903(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-
CC         phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
CC         inositol-4,5-bisphosphate) + ADP + H(+); Xref=Rhea:RHEA:14425,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:58178,
CC         ChEBI:CHEBI:58456, ChEBI:CHEBI:456216; EC=2.7.1.68;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q8RY89-1; Sequence=Displayed;
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG51639.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC018908; AAG51639.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE33744.1; -; Genomic_DNA.
DR   EMBL; AY074507; AAL69491.1; -; mRNA.
DR   EMBL; AY133824; AAM91758.1; -; mRNA.
DR   PIR; D96634; D96634.
DR   RefSeq; NP_176286.2; NM_104770.5. [Q8RY89-1]
DR   AlphaFoldDB; Q8RY89; -.
DR   SMR; Q8RY89; -.
DR   BioGRID; 27605; 1.
DR   STRING; 3702.Q8RY89; -.
DR   iPTMnet; Q8RY89; -.
DR   PaxDb; 3702-AT1G60890-2; -.
DR   ProteomicsDB; 234956; -. [Q8RY89-1]
DR   EnsemblPlants; AT1G60890.1; AT1G60890.1; AT1G60890. [Q8RY89-1]
DR   GeneID; 842381; -.
DR   Gramene; AT1G60890.1; AT1G60890.1; AT1G60890. [Q8RY89-1]
DR   KEGG; ath:AT1G60890; -.
DR   Araport; AT1G60890; -.
DR   TAIR; AT1G60890; -.
DR   eggNOG; KOG0229; Eukaryota.
DR   HOGENOM; CLU_004312_6_4_1; -.
DR   InParanoid; Q8RY89; -.
DR   OMA; CSINFYW; -.
DR   PhylomeDB; Q8RY89; -.
DR   BioCyc; ARA:AT1G60890-MONOMER; -.
DR   PRO; PR:Q8RY89; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q8RY89; baseline and differential.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0016308; F:1-phosphatidylinositol-4-phosphate 5-kinase activity; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046854; P:phosphatidylinositol phosphate biosynthetic process; IBA:GO_Central.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   CDD; cd17302; PIPKc_AtPIP5K_like; 1.
DR   Gene3D; 3.30.810.10; 2-Layer Sandwich; 1.
DR   Gene3D; 2.20.110.10; Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain; 3.
DR   Gene3D; 3.30.800.10; Phosphatidylinositol Phosphate Kinase II Beta; 1.
DR   InterPro; IPR003409; MORN.
DR   InterPro; IPR017163; PIno-4-P-5_kinase_pln.
DR   InterPro; IPR027483; PInositol-4-P-4/5-kinase_C_sf.
DR   InterPro; IPR002498; PInositol-4-P-4/5-kinase_core.
DR   InterPro; IPR027484; PInositol-4-P-5-kinase_N.
DR   InterPro; IPR023610; PInositol-4/5-P-5/4-kinase.
DR   PANTHER; PTHR23086:SF25; PHOSPHATIDYLINOSITOL 4-PHOSPHATE 5-KINASE 8; 1.
DR   PANTHER; PTHR23086; PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE; 1.
DR   Pfam; PF02493; MORN; 8.
DR   Pfam; PF01504; PIP5K; 1.
DR   PIRSF; PIRSF037274; PIP5K_plant_prd; 1.
DR   SMART; SM00698; MORN; 8.
DR   SMART; SM00330; PIPKc; 1.
DR   SUPFAM; SSF82185; Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain; 1.
DR   SUPFAM; SSF56104; SAICAR synthase-like; 1.
DR   PROSITE; PS51455; PIPK; 1.
DR   Genevisible; Q8RY89; AT.
PE   1: Evidence at protein level;
KW   Alternative splicing; ATP-binding; Kinase; Nucleotide-binding;
KW   Reference proteome; Repeat; Transferase.
FT   CHAIN           1..769
FT                   /note="Phosphatidylinositol 4-phosphate 5-kinase 8"
FT                   /id="PRO_0000185480"
FT   REPEAT          16..38
FT                   /note="MORN 1"
FT   REPEAT          39..61
FT                   /note="MORN 2"
FT   REPEAT          62..84
FT                   /note="MORN 3"
FT   REPEAT          85..107
FT                   /note="MORN 4"
FT   REPEAT          108..130
FT                   /note="MORN 5"
FT   REPEAT          131..153
FT                   /note="MORN 6"
FT   REPEAT          154..176
FT                   /note="MORN 7"
FT   REPEAT          177..198
FT                   /note="MORN 8"
FT   DOMAIN          344..765
FT                   /note="PIPK"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00781"
FT   REGION          266..289
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          725..746
FT                   /note="Activation loop"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   769 AA;  87403 MW;  2E8A045211FB39A3 CRC64;
     METRPAERVF SNGDVYSGQL KGTLPHGKGK YAWPDGIIYE GDWEEGKISG RGKLMWSSGA
     KYEGDFSGGY LHGFGTLTSP DGSVYAGAWR MNVRHGLGRK EYCNSDVYDG SWREGLQDGS
     GSYSWYNGNR FIGNWKKGKM SGRGVMSWAN GDLFNGFWLN GLRHGSGVYK YADGGFYFGT
     WSRGLKDGSG VFYPAGSKHP SLKKWHRHFG YDDTGNFLLS HNSTINIDDL RTSKAVSRSL
     SELTTTSGLT RTSERYPDDY WSTSDPPRDF MHHGPSSKSA RSVDSGQSEI RDKNPIVFER
     EYMQGVLIKE RIMSSIDMSH RARPLNLTKE VTVSACVSFL GGKWNHYLML NLQLGIRYTV
     GKITPVPPRE VRASDFSERA RIMMFFPRNG SQYTPPHKSI DFDWKDYCPM VFRNLREMFK
     LDAADYMMSI CGDDGLREIS SPGKSGSIFY LSHDDRFVIK TLKRSELKVL LRMLPRYYEH
     VGDYENTLIT KFFGVHRIKL KWGKKVRFVV MGNMFCTELK IHRRYDLKGS TQGRYTEKNK
     IGEKTTLKDL DLAYEFHMDK LLREALFKQI ILDCSFLESL QILDYSLLLG LHFRAPDPLT
     DILEPPNEMS DQESDSVGSV DVNLPREPSI PPKGLLMVTH EPNSVNTAPG PHIRGSTLRA
     FSVGEKEVDL ILPGTARLRV QLGVNMPAQA HHKLGQDNEE SGTVELFEVY DVVVYMGIID
     ILQEYNMKKK VEHTCKSMKY DPMTISAIEP TLYSKRFIDF LLKVFPEKA
//