Q8RY89 (PI5K8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 4-phosphate 5-kinase 8
Short name=AtPIP5K8
EC=2.7.1.68

Alternative name(s):
1-phosphatidylinositol 4-phosphate kinase 8
Diphosphoinositide kinase 8
PtdIns(4)P-5-kinase 8

Gene names

Name:	PIP5K8
Ordered Locus Names:	At1g60890
ORF Names:	T7P1.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	769 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
Sequence similarities	Contains 8 MORN repeats. Contains 1 PIPK domain.
Sequence caution	The sequence AAG51639.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphatidylinositol phosphorylation Inferred from electronic annotation. Source: GOC
Molecular_function	1-phosphatidylinositol-4-phosphate 5-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8RY89-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 769	769	Phosphatidylinositol 4-phosphate 5-kinase 8		PRO_0000185480
Regions
	Repeat	16 – 38	23	MORN 1
	Repeat	39 – 61	23	MORN 2
	Repeat	62 – 84	23	MORN 3
	Repeat	85 – 107	23	MORN 4
	Repeat	108 – 130	23	MORN 5
	Repeat	131 – 153	23	MORN 6
	Repeat	154 – 176	23	MORN 7
	Repeat	177 – 198	22	MORN 8
	Domain	344 – 765	422	PIPK
	Region	725 – 746	22	Activation loop By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 2E8A045211FB39A3	FASTA	769	87,403
	10 20 30 40 50 60 METRPAERVF SNGDVYSGQL KGTLPHGKGK YAWPDGIIYE GDWEEGKISG RGKLMWSSGA 70 80 90 100 110 120 KYEGDFSGGY LHGFGTLTSP DGSVYAGAWR MNVRHGLGRK EYCNSDVYDG SWREGLQDGS 130 140 150 160 170 180 GSYSWYNGNR FIGNWKKGKM SGRGVMSWAN GDLFNGFWLN GLRHGSGVYK YADGGFYFGT 190 200 210 220 230 240 WSRGLKDGSG VFYPAGSKHP SLKKWHRHFG YDDTGNFLLS HNSTINIDDL RTSKAVSRSL 250 260 270 280 290 300 SELTTTSGLT RTSERYPDDY WSTSDPPRDF MHHGPSSKSA RSVDSGQSEI RDKNPIVFER 310 320 330 340 350 360 EYMQGVLIKE RIMSSIDMSH RARPLNLTKE VTVSACVSFL GGKWNHYLML NLQLGIRYTV 370 380 390 400 410 420 GKITPVPPRE VRASDFSERA RIMMFFPRNG SQYTPPHKSI DFDWKDYCPM VFRNLREMFK 430 440 450 460 470 480 LDAADYMMSI CGDDGLREIS SPGKSGSIFY LSHDDRFVIK TLKRSELKVL LRMLPRYYEH 490 500 510 520 530 540 VGDYENTLIT KFFGVHRIKL KWGKKVRFVV MGNMFCTELK IHRRYDLKGS TQGRYTEKNK 550 560 570 580 590 600 IGEKTTLKDL DLAYEFHMDK LLREALFKQI ILDCSFLESL QILDYSLLLG LHFRAPDPLT 610 620 630 640 650 660 DILEPPNEMS DQESDSVGSV DVNLPREPSI PPKGLLMVTH EPNSVNTAPG PHIRGSTLRA 670 680 690 700 710 720 FSVGEKEVDL ILPGTARLRV QLGVNMPAQA HHKLGQDNEE SGTVELFEVY DVVVYMGIID 730 740 750 760 ILQEYNMKKK VEHTCKSMKY DPMTISAIEP TLYSKRFIDF LLKVFPEKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C." Mueller-Roeber B., Pical C. Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC018908 Genomic DNA. Translation: AAG51639.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE33744.1. AY074507 mRNA. Translation: AAL69491.1. AY133824 mRNA. Translation: AAM91758.1.
IPI	IPI00547011.
PIR	D96634.
RefSeq	NP_176286.2. NM_104770.4.
UniGene	At.28792.
3D structure databases
ProteinModelPortal	Q8RY89.
SMR	Q8RY89. Positions 352-592.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G60890.1-P.
Proteomic databases
PaxDb	Q8RY89.
PRIDE	Q8RY89.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G60890.1; AT1G60890.1; AT1G60890.
GeneID	842381.
KEGG	ath:AT1G60890.
Organism-specific databases
TAIR	At1g60890.
Phylogenomic databases
eggNOG	COG4642.
HOGENOM	HOG000193875.
InParanoid	Q8RY89.
KO	K00889.
PhylomeDB	Q8RY89.
ProtClustDB	CLSN2690395.
Gene expression databases
ArrayExpress	Q8RY89.
Genevestigator	Q8RY89.
GermOnline	AT1G60890. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.30.800.10. 1 hit. 3.30.810.10. 2 hits.
InterPro	IPR003409. MORN. IPR017163. PIno-4-P-5_kinase_pln. IPR023610. PInositol-4-P-5-kinase. IPR027483. PInositol-4-P-5-kinase_C. IPR002498. PInositol-4-P-5-kinase_core. IPR027484. PInositol-4-P-5-kinase_N. IPR016034. PInositol-4P-5-kinase_core_sub. [Graphical view]
PANTHER	PTHR23086. PTHR23086. 1 hit.
Pfam	PF02493. MORN. 8 hits. PF01504. PIP5K. 1 hit. [Graphical view]
PIRSF	PIRSF037274. PIP5K_plant_prd. 1 hit.
SMART	SM00698. MORN. 8 hits. SM00330. PIPKc. 1 hit. [Graphical view]
PROSITE	PS51455. PIPK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PI5K8_ARATH

Accession

Primary (citable) accession number: Q8RY89
Secondary accession number(s): Q9C962

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	June 1, 2002
Last modified:	May 29, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program