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Q8RY89 (PI5K8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylinositol 4-phosphate 5-kinase 8

Short name=AtPIP5K8
EC=2.7.1.68
Alternative name(s):
1-phosphatidylinositol 4-phosphate kinase 8
Diphosphoinositide kinase 8
PtdIns(4)P-5-kinase 8
Gene names
Name:PIP5K8
Ordered Locus Names:At1g60890
ORF Names:T7P1.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length769 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

Sequence similarities

Contains 8 MORN repeats.

Contains 1 PIPK domain.

Sequence caution

The sequence AAG51639.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainRepeat
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_function1-phosphatidylinositol-4-phosphate 5-kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q8RY89-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 769769Phosphatidylinositol 4-phosphate 5-kinase 8
PRO_0000185480

Regions

Repeat16 – 3823MORN 1
Repeat39 – 6123MORN 2
Repeat62 – 8423MORN 3
Repeat85 – 10723MORN 4
Repeat108 – 13023MORN 5
Repeat131 – 15323MORN 6
Repeat154 – 17623MORN 7
Repeat177 – 19822MORN 8
Domain344 – 765422PIPK
Region725 – 74622Activation loop By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 2E8A045211FB39A3

FASTA76987,403
        10         20         30         40         50         60 
METRPAERVF SNGDVYSGQL KGTLPHGKGK YAWPDGIIYE GDWEEGKISG RGKLMWSSGA 

        70         80         90        100        110        120 
KYEGDFSGGY LHGFGTLTSP DGSVYAGAWR MNVRHGLGRK EYCNSDVYDG SWREGLQDGS 

       130        140        150        160        170        180 
GSYSWYNGNR FIGNWKKGKM SGRGVMSWAN GDLFNGFWLN GLRHGSGVYK YADGGFYFGT 

       190        200        210        220        230        240 
WSRGLKDGSG VFYPAGSKHP SLKKWHRHFG YDDTGNFLLS HNSTINIDDL RTSKAVSRSL 

       250        260        270        280        290        300 
SELTTTSGLT RTSERYPDDY WSTSDPPRDF MHHGPSSKSA RSVDSGQSEI RDKNPIVFER 

       310        320        330        340        350        360 
EYMQGVLIKE RIMSSIDMSH RARPLNLTKE VTVSACVSFL GGKWNHYLML NLQLGIRYTV 

       370        380        390        400        410        420 
GKITPVPPRE VRASDFSERA RIMMFFPRNG SQYTPPHKSI DFDWKDYCPM VFRNLREMFK 

       430        440        450        460        470        480 
LDAADYMMSI CGDDGLREIS SPGKSGSIFY LSHDDRFVIK TLKRSELKVL LRMLPRYYEH 

       490        500        510        520        530        540 
VGDYENTLIT KFFGVHRIKL KWGKKVRFVV MGNMFCTELK IHRRYDLKGS TQGRYTEKNK 

       550        560        570        580        590        600 
IGEKTTLKDL DLAYEFHMDK LLREALFKQI ILDCSFLESL QILDYSLLLG LHFRAPDPLT 

       610        620        630        640        650        660 
DILEPPNEMS DQESDSVGSV DVNLPREPSI PPKGLLMVTH EPNSVNTAPG PHIRGSTLRA 

       670        680        690        700        710        720 
FSVGEKEVDL ILPGTARLRV QLGVNMPAQA HHKLGQDNEE SGTVELFEVY DVVVYMGIID 

       730        740        750        760 
ILQEYNMKKK VEHTCKSMKY DPMTISAIEP TLYSKRFIDF LLKVFPEKA 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
Mueller-Roeber B., Pical C.
Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[5]"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC018908 Genomic DNA. Translation: AAG51639.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33744.1.
AY074507 mRNA. Translation: AAL69491.1.
AY133824 mRNA. Translation: AAM91758.1.
PIRD96634.
RefSeqNP_176286.2. NM_104770.4. [Q8RY89-1]
UniGeneAt.28792.

3D structure databases

ProteinModelPortalQ8RY89.
SMRQ8RY89. Positions 352-592.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid27605. 1 interaction.
STRING3702.AT1G60890.1-P.

Proteomic databases

PaxDbQ8RY89.
PRIDEQ8RY89.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G60890.1; AT1G60890.1; AT1G60890. [Q8RY89-1]
GeneID842381.
KEGGath:AT1G60890.

Organism-specific databases

TAIRAT1G60890.

Phylogenomic databases

eggNOGCOG4642.
HOGENOMHOG000193875.
InParanoidQ8RY89.
KOK00889.
PhylomeDBQ8RY89.

Enzyme and pathway databases

BioCycARA:AT1G60890-MONOMER.
ARA:GQT-2287-MONOMER.

Gene expression databases

ArrayExpressQ8RY89.
GenevestigatorQ8RY89.

Family and domain databases

Gene3D3.30.800.10. 1 hit.
3.30.810.10. 2 hits.
InterProIPR003409. MORN.
IPR017163. PIno-4-P-5_kinase_pln.
IPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
IPR016034. PInositol-4P-5-kinase_core_sub.
[Graphical view]
PANTHERPTHR23086. PTHR23086. 1 hit.
PfamPF02493. MORN. 8 hits.
PF01504. PIP5K. 1 hit.
[Graphical view]
PIRSFPIRSF037274. PIP5K_plant_prd. 1 hit.
SMARTSM00698. MORN. 8 hits.
SM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEPS51455. PIPK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePI5K8_ARATH
AccessionPrimary (citable) accession number: Q8RY89
Secondary accession number(s): Q9C962
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names