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Q8RY89

- PI5K8_ARATH

UniProt

Q8RY89 - PI5K8_ARATH

Protein

Phosphatidylinositol 4-phosphate 5-kinase 8

Gene

PIP5K8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

    GO - Molecular functioni

    1. 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G60890-MONOMER.
    ARA:GQT-2287-MONOMER.
    ReactomeiREACT_187642. Synthesis of PIPs at the plasma membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol 4-phosphate 5-kinase 8 (EC:2.7.1.68)
    Short name:
    AtPIP5K8
    Alternative name(s):
    1-phosphatidylinositol 4-phosphate kinase 8
    Diphosphoinositide kinase 8
    PtdIns(4)P-5-kinase 8
    Gene namesi
    Name:PIP5K8
    Ordered Locus Names:At1g60890
    ORF Names:T7P1.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G60890.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 769769Phosphatidylinositol 4-phosphate 5-kinase 8PRO_0000185480Add
    BLAST

    Proteomic databases

    PaxDbiQ8RY89.
    PRIDEiQ8RY89.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8RY89.
    GenevestigatoriQ8RY89.

    Interactioni

    Protein-protein interaction databases

    BioGridi27605. 1 interaction.
    STRINGi3702.AT1G60890.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8RY89.
    SMRiQ8RY89. Positions 352-592.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati16 – 3823MORN 1Add
    BLAST
    Repeati39 – 6123MORN 2Add
    BLAST
    Repeati62 – 8423MORN 3Add
    BLAST
    Repeati85 – 10723MORN 4Add
    BLAST
    Repeati108 – 13023MORN 5Add
    BLAST
    Repeati131 – 15323MORN 6Add
    BLAST
    Repeati154 – 17623MORN 7Add
    BLAST
    Repeati177 – 19822MORN 8Add
    BLAST
    Domaini344 – 765422PIPKPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni725 – 74622Activation loopBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 8 MORN repeats.Curated
    Contains 1 PIPK domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG4642.
    HOGENOMiHOG000193875.
    InParanoidiQ8RY89.
    KOiK00889.
    PhylomeDBiQ8RY89.

    Family and domain databases

    Gene3Di3.30.800.10. 1 hit.
    3.30.810.10. 2 hits.
    InterProiIPR003409. MORN.
    IPR017163. PIno-4-P-5_kinase_pln.
    IPR023610. PInositol-4-P-5-kinase.
    IPR027483. PInositol-4-P-5-kinase_C.
    IPR002498. PInositol-4-P-5-kinase_core.
    IPR027484. PInositol-4-P-5-kinase_N.
    IPR016034. PInositol-4P-5-kinase_core_sub.
    [Graphical view]
    PANTHERiPTHR23086. PTHR23086. 1 hit.
    PfamiPF02493. MORN. 8 hits.
    PF01504. PIP5K. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037274. PIP5K_plant_prd. 1 hit.
    SMARTiSM00698. MORN. 8 hits.
    SM00330. PIPKc. 1 hit.
    [Graphical view]
    PROSITEiPS51455. PIPK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q8RY89-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    METRPAERVF SNGDVYSGQL KGTLPHGKGK YAWPDGIIYE GDWEEGKISG    50
    RGKLMWSSGA KYEGDFSGGY LHGFGTLTSP DGSVYAGAWR MNVRHGLGRK 100
    EYCNSDVYDG SWREGLQDGS GSYSWYNGNR FIGNWKKGKM SGRGVMSWAN 150
    GDLFNGFWLN GLRHGSGVYK YADGGFYFGT WSRGLKDGSG VFYPAGSKHP 200
    SLKKWHRHFG YDDTGNFLLS HNSTINIDDL RTSKAVSRSL SELTTTSGLT 250
    RTSERYPDDY WSTSDPPRDF MHHGPSSKSA RSVDSGQSEI RDKNPIVFER 300
    EYMQGVLIKE RIMSSIDMSH RARPLNLTKE VTVSACVSFL GGKWNHYLML 350
    NLQLGIRYTV GKITPVPPRE VRASDFSERA RIMMFFPRNG SQYTPPHKSI 400
    DFDWKDYCPM VFRNLREMFK LDAADYMMSI CGDDGLREIS SPGKSGSIFY 450
    LSHDDRFVIK TLKRSELKVL LRMLPRYYEH VGDYENTLIT KFFGVHRIKL 500
    KWGKKVRFVV MGNMFCTELK IHRRYDLKGS TQGRYTEKNK IGEKTTLKDL 550
    DLAYEFHMDK LLREALFKQI ILDCSFLESL QILDYSLLLG LHFRAPDPLT 600
    DILEPPNEMS DQESDSVGSV DVNLPREPSI PPKGLLMVTH EPNSVNTAPG 650
    PHIRGSTLRA FSVGEKEVDL ILPGTARLRV QLGVNMPAQA HHKLGQDNEE 700
    SGTVELFEVY DVVVYMGIID ILQEYNMKKK VEHTCKSMKY DPMTISAIEP 750
    TLYSKRFIDF LLKVFPEKA 769
    Length:769
    Mass (Da):87,403
    Last modified:June 1, 2002 - v1
    Checksum:i2E8A045211FB39A3
    GO

    Sequence cautioni

    The sequence AAG51639.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC018908 Genomic DNA. Translation: AAG51639.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE33744.1.
    AY074507 mRNA. Translation: AAL69491.1.
    AY133824 mRNA. Translation: AAM91758.1.
    PIRiD96634.
    RefSeqiNP_176286.2. NM_104770.4. [Q8RY89-1]
    UniGeneiAt.28792.

    Genome annotation databases

    EnsemblPlantsiAT1G60890.1; AT1G60890.1; AT1G60890. [Q8RY89-1]
    GeneIDi842381.
    KEGGiath:AT1G60890.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC018908 Genomic DNA. Translation: AAG51639.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE33744.1 .
    AY074507 mRNA. Translation: AAL69491.1 .
    AY133824 mRNA. Translation: AAM91758.1 .
    PIRi D96634.
    RefSeqi NP_176286.2. NM_104770.4. [Q8RY89-1 ]
    UniGenei At.28792.

    3D structure databases

    ProteinModelPortali Q8RY89.
    SMRi Q8RY89. Positions 352-592.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 27605. 1 interaction.
    STRINGi 3702.AT1G60890.1-P.

    Proteomic databases

    PaxDbi Q8RY89.
    PRIDEi Q8RY89.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G60890.1 ; AT1G60890.1 ; AT1G60890 . [Q8RY89-1 ]
    GeneIDi 842381.
    KEGGi ath:AT1G60890.

    Organism-specific databases

    TAIRi AT1G60890.

    Phylogenomic databases

    eggNOGi COG4642.
    HOGENOMi HOG000193875.
    InParanoidi Q8RY89.
    KOi K00889.
    PhylomeDBi Q8RY89.

    Enzyme and pathway databases

    BioCyci ARA:AT1G60890-MONOMER.
    ARA:GQT-2287-MONOMER.
    Reactomei REACT_187642. Synthesis of PIPs at the plasma membrane.

    Gene expression databases

    ArrayExpressi Q8RY89.
    Genevestigatori Q8RY89.

    Family and domain databases

    Gene3Di 3.30.800.10. 1 hit.
    3.30.810.10. 2 hits.
    InterProi IPR003409. MORN.
    IPR017163. PIno-4-P-5_kinase_pln.
    IPR023610. PInositol-4-P-5-kinase.
    IPR027483. PInositol-4-P-5-kinase_C.
    IPR002498. PInositol-4-P-5-kinase_core.
    IPR027484. PInositol-4-P-5-kinase_N.
    IPR016034. PInositol-4P-5-kinase_core_sub.
    [Graphical view ]
    PANTHERi PTHR23086. PTHR23086. 1 hit.
    Pfami PF02493. MORN. 8 hits.
    PF01504. PIP5K. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037274. PIP5K_plant_prd. 1 hit.
    SMARTi SM00698. MORN. 8 hits.
    SM00330. PIPKc. 1 hit.
    [Graphical view ]
    PROSITEi PS51455. PIPK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
      Mueller-Roeber B., Pical C.
      Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    5. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPI5K8_ARATH
    AccessioniPrimary (citable) accession number: Q8RY89
    Secondary accession number(s): Q9C962
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3