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Protein

Polygalacturonase ADPG2

Gene

ADPG2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Polygalacturonase involved in cell separation in the final stages of pod shatter, in anther dehiscence and in floral organ abscission.2 Publications

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei264Proton donorPROSITE-ProRule annotation1
Active sitei287PROSITE-ProRule annotation1

GO - Molecular functioni

  • polygalacturonase activity Source: TAIR

GO - Biological processi

  • anther dehiscence Source: TAIR
  • carbohydrate metabolic process Source: InterPro
  • cell wall modification involved in abscission Source: TAIR
  • floral organ abscission Source: TAIR
  • fruit dehiscence Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT2G41850-MONOMER.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Polygalacturonase ADPG2 (EC:3.2.1.15)
Short name:
AtADPG2
Short name:
PG ADPG2
Alternative name(s):
Pectinase ADPG2
Protein ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2
Gene namesi
Name:ADPG2
Synonyms:PGAZAT
Ordered Locus Names:At2g41850
ORF Names:T11A7.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G41850.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions. Small delay in floral organ shedding.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000036791425 – 433Polygalacturonase ADPG2Add BLAST409

Proteomic databases

PaxDbiQ8RY29.
PRIDEiQ8RY29.

Expressioni

Tissue specificityi

Expressed in roots and in the abscission zone of the sepals, petals and stamens of flowers, at the base of cauline leaves and in the basal cell of trichomes from senescing leaves. Found at the site of lateral root emergence, in the dehiscence zone of anthers and maturing siliques. Also expressed early in anther development, at the time of microspore separation. Expressed in germinating seeds, at the point at which the radicle broke through the seed coat. Not expressed at the junction between the seed and the funiculus or in the dehiscence zone of anthers or pods.4 Publications

Inductioni

Up-regulated by ethylene.2 Publications

Gene expression databases

GenevisibleiQ8RY29. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G41850.1.

Structurei

3D structure databases

ProteinModelPortaliQ8RY29.
SMRiQ8RY29.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati223 – 249PbH1 1Add BLAST27
Repeati250 – 271PbH1 2Add BLAST22
Repeati273 – 293PbH1 3Add BLAST21
Repeati303 – 324PbH1 4Add BLAST22
Repeati332 – 353PbH1 5Add BLAST22

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 5 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IF8F. Eukaryota.
COG5434. LUCA.
HOGENOMiHOG000237792.
InParanoidiQ8RY29.
OMAiIKLMIEG.
OrthoDBiEOG09360ADQ.
PhylomeDBiQ8RY29.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8RY29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARCTNLVTV FLLWALLMFS WCKASRISPN VYDHSYKRFK SDSLIKRRED
60 70 80 90 100
ITGLRSFVRA SLRTPTTVSV SDFGAKGDGK TDDTQAFVNA WKKACSSNGA
110 120 130 140 150
VNLLVPKGNT YLLKSIQLTG PCNSILTVQI FGTLSASQKR SDYKDISKWI
160 170 180 190 200
MFDGVNNLSV DGGDTGVVDG NGETWWQNSC KRNKAKPCTK APTALTFYNS
210 220 230 240 250
KSLIVKNLKV RNAQQIQISI EKCSNVQVSN VVVTAPADSP NTDGIHITNT
260 270 280 290 300
QNIRVSESII GTGDDCISIE SGSQNVQIND ITCGPGHGIS IGSLGDDNSK
310 320 330 340 350
AFVSGVTVDG AKLSGTDNGV RIKTYQGGSG TASNIIFQNI QMDNVKNPII
360 370 380 390 400
IDQDYCDKSK CTTEKSAVQV KNVVYRDISG TSASENAITF NCSKNYPCQG
410 420 430
IVLDRVNIKG GKATCTNANV VDKGAVLPQC NST
Length:433
Mass (Da):46,623
Last modified:April 14, 2009 - v2
Checksum:i11311B0DBE3FCD2D
GO

Sequence cautioni

The sequence AAC02763 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5T → S in AAN60351 (Ref. 4) Curated1
Sequence conflicti19F → L in AAL84942 (PubMed:14593172).Curated1
Sequence conflicti33D → G in AAN60351 (Ref. 4) Curated1
Sequence conflicti157N → S in AAN60351 (Ref. 4) Curated1
Sequence conflicti177Q → P in AAN60351 (Ref. 4) Curated1
Sequence conflicti181K → Q in AAN60351 (Ref. 4) Curated1
Sequence conflicti355Y → D in AAL84942 (PubMed:14593172).Curated1
Sequence conflicti359S → G in AAL84942 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002339 Genomic DNA. Translation: AAC02763.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10040.1.
AY078936 mRNA. Translation: AAL84942.1.
AF083793 mRNA. Translation: AAN60351.1.
PIRiH84846.
RefSeqiNP_850359.1. NM_180028.3.
UniGeneiAt.42781.

Genome annotation databases

EnsemblPlantsiAT2G41850.1; AT2G41850.1; AT2G41850.
GeneIDi818785.
GrameneiAT2G41850.1; AT2G41850.1; AT2G41850.
KEGGiath:AT2G41850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002339 Genomic DNA. Translation: AAC02763.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10040.1.
AY078936 mRNA. Translation: AAL84942.1.
AF083793 mRNA. Translation: AAN60351.1.
PIRiH84846.
RefSeqiNP_850359.1. NM_180028.3.
UniGeneiAt.42781.

3D structure databases

ProteinModelPortaliQ8RY29.
SMRiQ8RY29.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G41850.1.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Proteomic databases

PaxDbiQ8RY29.
PRIDEiQ8RY29.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G41850.1; AT2G41850.1; AT2G41850.
GeneIDi818785.
GrameneiAT2G41850.1; AT2G41850.1; AT2G41850.
KEGGiath:AT2G41850.

Organism-specific databases

TAIRiAT2G41850.

Phylogenomic databases

eggNOGiENOG410IF8F. Eukaryota.
COG5434. LUCA.
HOGENOMiHOG000237792.
InParanoidiQ8RY29.
OMAiIKLMIEG.
OrthoDBiEOG09360ADQ.
PhylomeDBiQ8RY29.

Enzyme and pathway databases

BioCyciARA:AT2G41850-MONOMER.

Miscellaneous databases

PROiQ8RY29.

Gene expression databases

GenevisibleiQ8RY29. AT.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADPG2_ARATH
AccessioniPrimary (citable) accession number: Q8RY29
Secondary accession number(s): O22935, Q8H782
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: November 30, 2016
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.