Q8RY29 (ADPG2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polygalacturonase ADPG2
Short name=AtADPG2
Short name=PG ADPG2
EC=3.2.1.15

Alternative name(s):
Pectinase ADPG2
Protein ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2

Gene names

Name:	ADPG2
Synonyms:	PGAZAT
Ordered Locus Names:	At2g41850
ORF Names:	T11A7.5

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Polygalacturonase involved in cell separation in the final stages of pod shatter, in anther dehiscence and in floral organ abscission. Ref.7 Ref.8
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted › cell wall By similarity.
Tissue specificity	Expressed in roots and in the abscission zone of the sepals, petals and stamens of flowers, at the base of cauline leaves and in the basal cell of trichomes from senescing leaves. Found at the site of lateral root emergence, in the dehiscence zone of anthers and maturing siliques. Also expressed early in anther development, at the time of microspore separation. Expressed in germinating seeds, at the point at which the radicle broke through the seed coat. Not expressed at the junction between the seed and the funiculus or in the dehiscence zone of anthers or pods. Ref.5 Ref.6 Ref.7 Ref.8
Induction	Up-regulated by ethylene. Ref.5 Ref.7
Disruption phenotype	No visible phenotype under normal growth conditions. Small delay in floral organ shedding. Ref.7 Ref.8
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 5 PbH1 repeats.
Sequence caution	The sequence AAC02763.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anther dehiscence Inferred from mutant phenotype Ref.8. Source: TAIR carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall modification involved in abscission Traceable author statement Ref.8. Source: TAIR floral organ abscission Inferred from mutant phenotype Ref.8. Source: TAIR fruit dehiscence Inferred from mutant phenotype Ref.8. Source: TAIR
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	polygalacturonase activity Inferred from direct assay Ref.8. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 433	409	Polygalacturonase ADPG2		PRO_0000367914
Regions
	Repeat	223 – 249	27	PbH1 1
	Repeat	250 – 271	22	PbH1 2
	Repeat	273 – 293	21	PbH1 3
	Repeat	303 – 324	22	PbH1 4
	Repeat	332 – 353	22	PbH1 5
Sites
	Active site	264	1	Proton donor By similarity
	Active site	287	1	By similarity
Experimental info
	Sequence conflict	5	1	T → S in AAN60351. Ref.4
	Sequence conflict	19	1	F → L in AAL84942. Ref.3
	Sequence conflict	33	1	D → G in AAN60351. Ref.4
	Sequence conflict	157	1	N → S in AAN60351. Ref.4
	Sequence conflict	177	1	Q → P in AAN60351. Ref.4
	Sequence conflict	181	1	K → Q in AAN60351. Ref.4
	Sequence conflict	355	1	Y → D in AAL84942. Ref.3
	Sequence conflict	359	1	S → G in AAL84942. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8RY29 [UniParc]. Last modified April 14, 2009. Version 2. Checksum: 11311B0DBE3FCD2D	FASTA	433	46,623
	10 20 30 40 50 60 MARCTNLVTV FLLWALLMFS WCKASRISPN VYDHSYKRFK SDSLIKRRED ITGLRSFVRA 70 80 90 100 110 120 SLRTPTTVSV SDFGAKGDGK TDDTQAFVNA WKKACSSNGA VNLLVPKGNT YLLKSIQLTG 130 140 150 160 170 180 PCNSILTVQI FGTLSASQKR SDYKDISKWI MFDGVNNLSV DGGDTGVVDG NGETWWQNSC 190 200 210 220 230 240 KRNKAKPCTK APTALTFYNS KSLIVKNLKV RNAQQIQISI EKCSNVQVSN VVVTAPADSP 250 260 270 280 290 300 NTDGIHITNT QNIRVSESII GTGDDCISIE SGSQNVQIND ITCGPGHGIS IGSLGDDNSK 310 320 330 340 350 360 AFVSGVTVDG AKLSGTDNGV RIKTYQGGSG TASNIIFQNI QMDNVKNPII IDQDYCDKSK 370 380 390 400 410 420 CTTEKSAVQV KNVVYRDISG TSASENAITF NCSKNYPCQG IVLDRVNIKG GKATCTNANV 430 VDKGAVLPQC NST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Signal peptide selection derived cDNAs from Arabidopsis thaliana leaves and guard cells." Stracke R., Palme K. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-185.
[5]	"Temporal and spatial expression of a polygalacturonase during leaf and flower abscission in oilseed rape and Arabidopsis." Gonzalez-Carranza Z.H., Whitelaw C.A., Swarup R., Roberts J.A. Plant Physiol. 128:534-543(2002) [PubMed: 11842157] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
[6]	"Patterns of expansion and expression divergence in the plant polygalacturonase gene family." Kim J., Shiu S.-H., Thoma S., Li W.-H., Patterson S.E. Genome Biol. 7:R87.1-R87.14(2006) [PubMed: 17010199] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Expression of polygalacturonases and evidence to support their role during cell separation processes in Arabidopsis thaliana." Gonzalez-Carranza Z.H., Elliott K.A., Roberts J.A. J. Exp. Bot. 58:3719-3730(2007) [PubMed: 17928369] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION.
[8]	"ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1 (ADPG1), ADPG2, and QUARTET2 are polygalacturonases required for cell separation during reproductive development in Arabidopsis." Ogawa M., Kay P., Wilson S., Swain S.M. Plant Cell 21:216-233(2009) [PubMed: 19168715] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002339 Genomic DNA. Translation: AAC02763.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC10040.1. AY078936 mRNA. Translation: AAL84942.1. AF083793 mRNA. Translation: AAN60351.1.
IPI	IPI00518873.
PIR	H84846.
RefSeq	NP_850359.1. NM_180028.2.
UniGene	At.42781.
3D structure databases
ProteinModelPortal	Q8RY29.
SMR	Q8RY29. Positions 64-421.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8RY29.
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Proteomic databases
PRIDE	Q8RY29.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G41850.1; AT2G41850.1; AT2G41850.
GeneID	818785.
GenomeReviews	Gene locus AT2G41850 in contig CT485783_GR.
KEGG	ath:AT2G41850.
Organism-specific databases
GeneFarm	30. 2.
TAIR	At2g41850.
Phylogenomic databases
eggNOG	euNOG04659.
GeneTree	EPGT00050000001253.
InParanoid	Q8RY29.
OMA	DGNGETW.
PhylomeDB	Q8RY29.
ProtClustDB	PLN02218.
Gene expression databases
Genevestigator	Q8RY29.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADPG2_ARATH

Accession

Primary (citable) accession number: Q8RY29
Secondary accession number(s): O22935, Q8H782

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 14, 2009
Last modified:	December 14, 2011
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program