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Protein

Probable low-specificity L-threonine aldolase 1

Gene

THA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Threonine aldolase involved in threonine degradation to glycine. May play a role in the removal of L-allo-threonine.2 Publications

Catalytic activityi

L-threonine = glycine + acetaldehyde.
L-allo-threonine = glycine + acetaldehyde.

Cofactori

Kineticsi

  1. KM=7.1 mM for L-threonine1 Publication

    Pathway: L-threonine degradation via aldolase pathway

    This protein is involved in step 1 of the subpathway that synthesizes acetaldehyde and glycine from L-threonine.
    Proteins known to be involved in this subpathway in this organism are:
    1. Probable low-specificity L-threonine aldolase 2 (THA2), Probable low-specificity L-threonine aldolase 1 (THA1)
    This subpathway is part of the pathway L-threonine degradation via aldolase pathway, which is itself part of Amino-acid degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetaldehyde and glycine from L-threonine, the pathway L-threonine degradation via aldolase pathway and in Amino-acid degradation.

    GO - Molecular functioni

    GO - Biological processi

    • glycine biosynthetic process Source: GO_Central
    • threonine catabolic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciARA:GQT-1115-MONOMER.
    ARA:GQT-1116-MONOMER.
    ARA:GQT-1117-MONOMER.
    ARA:GQT-1118-MONOMER.
    UniPathwayiUPA00044; UER00429.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable low-specificity L-threonine aldolase 1 (EC:4.1.2.48)
    Alternative name(s):
    Threonine aldolase 1
    Gene namesi
    Name:THA1
    Ordered Locus Names:At1g08630
    ORF Names:F22O13.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548 Componenti: Chromosome 1

    Organism-specific databases

    TAIRiAT1G08630.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth conditions, but mutant plants have 50-fold increase in seed Thr content and 2-fold decrease in seedling Gly content.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi114 – 1141G → R in tha1-1; loss of function; 50-fold increase in seed Thr content and 2-fold decrease in seedling Gly content. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 358358Probable low-specificity L-threonine aldolase 1PRO_0000428659Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei207 – 2071N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    PRIDEiQ8RXU4.

    Expressioni

    Tissue specificityi

    Expressed in root tips, seedlings, carpels and seeds.1 Publication

    Gene expression databases

    ExpressionAtlasiQ8RXU4. baseline and differential.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G08630.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8RXU4.
    SMRiQ8RXU4. Positions 6-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the threonine aldolase family.Curated

    Phylogenomic databases

    HOGENOMiHOG000219824.
    InParanoidiQ8RXU4.
    KOiK01620.
    OMAiISVMVHC.
    PhylomeDBiQ8RXU4.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR023603. Threonine_aldolase.
    [Graphical view]
    PfamiPF01212. Beta_elim_lyase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017617. Thr_aldolase. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q8RXU4-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MVMRSVDLRS DTVTRPTDAM REAMCNAEVD DDVLGYDPTA RRLEEEMAKM
    60 70 80 90 100
    MGKEAALFVP SGTMGNLISV MVHCDVRGSE VILGDNCHIH VYENGGISTI
    110 120 130 140 150
    GGVHPKTVKN EEDGTMDLEA IEAAIRDPKG STFYPSTRLI CLENTHANSG
    160 170 180 190 200
    GRCLSVEYTE KVGEIAKRHG VKLHIDGARL FNASIALGVP VHKLVKAADS
    210 220 230 240 250
    VQVCLSKGLG APVGSVIVGS QSFIEKAKTV RKTLGGGMRQ IGVLCAAALV
    260 270 280 290 300
    ALQENLPKLQ HDHKKAKLLA EGLNQMKGIR VNVAAVETNM IFMDMEDGSR
    310 320 330 340 350
    LTAEKLRKNL EENGILLIRG NSSRIRIVIH HQITTSDVHY TLSCFQQAML

    TMQEPSRT
    Length:358
    Mass (Da):38,942
    Last modified:June 1, 2002 - v1
    Checksum:iE38254B11A967514
    GO
    Isoform 2 (identifier: Q8RXU4-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         270-283: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:344
    Mass (Da):37,431
    Checksum:i3541C1DA6F33C8CA
    GO

    Sequence cautioni

    The sequence AAF99780.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti321 – 3211N → K in AAM63785 (Ref. 6) Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei270 – 28314Missing in isoform 2. 1 PublicationVSP_054082Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC003981 Genomic DNA. Translation: AAF99780.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28320.1.
    CP002684 Genomic DNA. Translation: AEE28321.1.
    CP002684 Genomic DNA. Translation: AEE28322.1.
    CP002684 Genomic DNA. Translation: AEE28323.1.
    CP002684 Genomic DNA. Translation: AEE28324.1.
    AY080670 mRNA. Translation: AAL86346.1.
    AY117227 mRNA. Translation: AAM51302.1.
    AK317034 mRNA. Translation: BAH19728.1.
    AK230078 mRNA. Translation: BAF01898.1.
    AY086734 mRNA. Translation: AAM63785.1.
    PIRiT00716.
    RefSeqiNP_001031001.2. NM_001035924.2. [Q8RXU4-1]
    NP_001077492.1. NM_001084023.1. [Q8RXU4-2]
    NP_563822.1. NM_100736.2. [Q8RXU4-1]
    NP_849614.1. NM_179283.2. [Q8RXU4-1]
    NP_849615.1. NM_179284.3. [Q8RXU4-1]
    UniGeneiAt.57057.

    Genome annotation databases

    EnsemblPlantsiAT1G08630.1; AT1G08630.1; AT1G08630. [Q8RXU4-1]
    AT1G08630.2; AT1G08630.2; AT1G08630. [Q8RXU4-1]
    AT1G08630.3; AT1G08630.3; AT1G08630. [Q8RXU4-1]
    AT1G08630.4; AT1G08630.4; AT1G08630. [Q8RXU4-1]
    AT1G08630.5; AT1G08630.5; AT1G08630. [Q8RXU4-2]
    GeneIDi837385.
    KEGGiath:AT1G08630.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC003981 Genomic DNA. Translation: AAF99780.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28320.1.
    CP002684 Genomic DNA. Translation: AEE28321.1.
    CP002684 Genomic DNA. Translation: AEE28322.1.
    CP002684 Genomic DNA. Translation: AEE28323.1.
    CP002684 Genomic DNA. Translation: AEE28324.1.
    AY080670 mRNA. Translation: AAL86346.1.
    AY117227 mRNA. Translation: AAM51302.1.
    AK317034 mRNA. Translation: BAH19728.1.
    AK230078 mRNA. Translation: BAF01898.1.
    AY086734 mRNA. Translation: AAM63785.1.
    PIRiT00716.
    RefSeqiNP_001031001.2. NM_001035924.2. [Q8RXU4-1]
    NP_001077492.1. NM_001084023.1. [Q8RXU4-2]
    NP_563822.1. NM_100736.2. [Q8RXU4-1]
    NP_849614.1. NM_179283.2. [Q8RXU4-1]
    NP_849615.1. NM_179284.3. [Q8RXU4-1]
    UniGeneiAt.57057.

    3D structure databases

    ProteinModelPortaliQ8RXU4.
    SMRiQ8RXU4. Positions 6-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT1G08630.1.

    Proteomic databases

    PRIDEiQ8RXU4.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT1G08630.1; AT1G08630.1; AT1G08630. [Q8RXU4-1]
    AT1G08630.2; AT1G08630.2; AT1G08630. [Q8RXU4-1]
    AT1G08630.3; AT1G08630.3; AT1G08630. [Q8RXU4-1]
    AT1G08630.4; AT1G08630.4; AT1G08630. [Q8RXU4-1]
    AT1G08630.5; AT1G08630.5; AT1G08630. [Q8RXU4-2]
    GeneIDi837385.
    KEGGiath:AT1G08630.

    Organism-specific databases

    TAIRiAT1G08630.

    Phylogenomic databases

    HOGENOMiHOG000219824.
    InParanoidiQ8RXU4.
    KOiK01620.
    OMAiISVMVHC.
    PhylomeDBiQ8RXU4.

    Enzyme and pathway databases

    UniPathwayiUPA00044; UER00429.
    BioCyciARA:GQT-1115-MONOMER.
    ARA:GQT-1116-MONOMER.
    ARA:GQT-1117-MONOMER.
    ARA:GQT-1118-MONOMER.

    Miscellaneous databases

    PROiQ8RXU4.

    Gene expression databases

    ExpressionAtlasiQ8RXU4. baseline and differential.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR023603. Threonine_aldolase.
    [Graphical view]
    PfamiPF01212. Beta_elim_lyase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017617. Thr_aldolase. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    6. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    7. "Application of a high-throughput HPLC-MS/MS assay to Arabidopsis mutant screening; evidence that threonine aldolase plays a role in seed nutritional quality."
      Jander G., Norris S.R., Joshi V., Fraga M., Rugg A., Yu S., Li L., Last R.L.
      Plant J. 39:465-475(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF GLY-114.
    8. "Two Arabidopsis threonine aldolases are nonredundant and compete with threonine deaminase for a common substrate pool."
      Joshi V., Laubengayer K.M., Schauer N., Fernie A.R., Jander G.
      Plant Cell 18:3564-3575(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-114.

    Entry informationi

    Entry nameiTHA1_ARATH
    AccessioniPrimary (citable) accession number: Q8RXU4
    Secondary accession number(s): B9DG61
    , Q2V4P5, Q8LC88, Q9FRS2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: June 1, 2002
    Last modified: June 24, 2015
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.