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Q8RXU4

- THA1_ARATH

UniProt

Q8RXU4 - THA1_ARATH

Protein

Probable low-specificity L-threonine aldolase 1

Gene

THA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Threonine aldolase involved in threonine degradation to glycine. May play a role in the removal of L-allo-threonine.2 Publications

    Catalytic activityi

    L-threonine = glycine + acetaldehyde.
    L-allo-threonine = glycine + acetaldehyde.

    Cofactori

    Pyridoxal phosphate.By similarity

    Kineticsi

    1. KM=7.1 mM for L-threonine1 Publication

    Pathwayi

    GO - Molecular functioni

    1. pyridoxal phosphate binding Source: InterPro
    2. threonine aldolase activity Source: TAIR

    GO - Biological processi

    1. threonine catabolic process Source: TAIR

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciARA:GQT-1115-MONOMER.
    ARA:GQT-1116-MONOMER.
    ARA:GQT-1117-MONOMER.
    ARA:GQT-1118-MONOMER.
    BRENDAi2.1.2.1. 302.
    UniPathwayiUPA00044; UER00429.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable low-specificity L-threonine aldolase 1 (EC:4.1.2.48)
    Alternative name(s):
    Threonine aldolase 1
    Gene namesi
    Name:THA1
    Ordered Locus Names:At1g08630
    ORF Names:F22O13.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G08630.

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth conditions, but mutant plants have 50-fold increase in seed Thr content and 2-fold decrease in seedling Gly content.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi114 – 1141G → R in tha1-1; loss of function; 50-fold increase in seed Thr content and 2-fold decrease in seedling Gly content. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 358358Probable low-specificity L-threonine aldolase 1PRO_0000428659Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei207 – 2071N6-(pyridoxal phosphate)lysineBy similarity

    Expressioni

    Tissue specificityi

    Expressed in root tips, seedlings, carpels and seeds.1 Publication

    Gene expression databases

    ArrayExpressiQ8RXU4.
    GenevestigatoriQ8RXU4.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8RXU4.
    SMRiQ8RXU4. Positions 6-347.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the threonine aldolase family.Curated

    Phylogenomic databases

    InParanoidiQ8RXU4.
    KOiK01620.
    OMAiEVGDEQI.
    PhylomeDBiQ8RXU4.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR023603. Threonine_aldolase.
    [Graphical view]
    PfamiPF01212. Beta_elim_lyase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017617. Thr_aldolase. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8RXU4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVMRSVDLRS DTVTRPTDAM REAMCNAEVD DDVLGYDPTA RRLEEEMAKM    50
    MGKEAALFVP SGTMGNLISV MVHCDVRGSE VILGDNCHIH VYENGGISTI 100
    GGVHPKTVKN EEDGTMDLEA IEAAIRDPKG STFYPSTRLI CLENTHANSG 150
    GRCLSVEYTE KVGEIAKRHG VKLHIDGARL FNASIALGVP VHKLVKAADS 200
    VQVCLSKGLG APVGSVIVGS QSFIEKAKTV RKTLGGGMRQ IGVLCAAALV 250
    ALQENLPKLQ HDHKKAKLLA EGLNQMKGIR VNVAAVETNM IFMDMEDGSR 300
    LTAEKLRKNL EENGILLIRG NSSRIRIVIH HQITTSDVHY TLSCFQQAML 350
    TMQEPSRT 358
    Length:358
    Mass (Da):38,942
    Last modified:June 1, 2002 - v1
    Checksum:iE38254B11A967514
    GO
    Isoform 2 (identifier: Q8RXU4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         270-283: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:344
    Mass (Da):37,431
    Checksum:i3541C1DA6F33C8CA
    GO

    Sequence cautioni

    The sequence AAF99780.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti321 – 3211N → K in AAM63785. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei270 – 28314Missing in isoform 2. 1 PublicationVSP_054082Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC003981 Genomic DNA. Translation: AAF99780.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28320.1.
    CP002684 Genomic DNA. Translation: AEE28321.1.
    CP002684 Genomic DNA. Translation: AEE28322.1.
    CP002684 Genomic DNA. Translation: AEE28323.1.
    CP002684 Genomic DNA. Translation: AEE28324.1.
    AY080670 mRNA. Translation: AAL86346.1.
    AY117227 mRNA. Translation: AAM51302.1.
    AK317034 mRNA. Translation: BAH19728.1.
    AK230078 mRNA. Translation: BAF01898.1.
    AY086734 mRNA. Translation: AAM63785.1.
    PIRiT00716.
    RefSeqiNP_001031001.2. NM_001035924.2. [Q8RXU4-1]
    NP_001077492.1. NM_001084023.1. [Q8RXU4-2]
    NP_563822.1. NM_100736.2. [Q8RXU4-1]
    NP_849614.1. NM_179283.2. [Q8RXU4-1]
    NP_849615.1. NM_179284.3. [Q8RXU4-1]
    UniGeneiAt.57057.

    Genome annotation databases

    EnsemblPlantsiAT1G08630.1; AT1G08630.1; AT1G08630. [Q8RXU4-1]
    AT1G08630.2; AT1G08630.2; AT1G08630. [Q8RXU4-1]
    AT1G08630.3; AT1G08630.3; AT1G08630. [Q8RXU4-1]
    AT1G08630.4; AT1G08630.4; AT1G08630. [Q8RXU4-1]
    AT1G08630.5; AT1G08630.5; AT1G08630. [Q8RXU4-2]
    GeneIDi837385.
    KEGGiath:AT1G08630.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC003981 Genomic DNA. Translation: AAF99780.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28320.1 .
    CP002684 Genomic DNA. Translation: AEE28321.1 .
    CP002684 Genomic DNA. Translation: AEE28322.1 .
    CP002684 Genomic DNA. Translation: AEE28323.1 .
    CP002684 Genomic DNA. Translation: AEE28324.1 .
    AY080670 mRNA. Translation: AAL86346.1 .
    AY117227 mRNA. Translation: AAM51302.1 .
    AK317034 mRNA. Translation: BAH19728.1 .
    AK230078 mRNA. Translation: BAF01898.1 .
    AY086734 mRNA. Translation: AAM63785.1 .
    PIRi T00716.
    RefSeqi NP_001031001.2. NM_001035924.2. [Q8RXU4-1 ]
    NP_001077492.1. NM_001084023.1. [Q8RXU4-2 ]
    NP_563822.1. NM_100736.2. [Q8RXU4-1 ]
    NP_849614.1. NM_179283.2. [Q8RXU4-1 ]
    NP_849615.1. NM_179284.3. [Q8RXU4-1 ]
    UniGenei At.57057.

    3D structure databases

    ProteinModelPortali Q8RXU4.
    SMRi Q8RXU4. Positions 6-347.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G08630.1 ; AT1G08630.1 ; AT1G08630 . [Q8RXU4-1 ]
    AT1G08630.2 ; AT1G08630.2 ; AT1G08630 . [Q8RXU4-1 ]
    AT1G08630.3 ; AT1G08630.3 ; AT1G08630 . [Q8RXU4-1 ]
    AT1G08630.4 ; AT1G08630.4 ; AT1G08630 . [Q8RXU4-1 ]
    AT1G08630.5 ; AT1G08630.5 ; AT1G08630 . [Q8RXU4-2 ]
    GeneIDi 837385.
    KEGGi ath:AT1G08630.

    Organism-specific databases

    TAIRi AT1G08630.

    Phylogenomic databases

    InParanoidi Q8RXU4.
    KOi K01620.
    OMAi EVGDEQI.
    PhylomeDBi Q8RXU4.

    Enzyme and pathway databases

    UniPathwayi UPA00044 ; UER00429 .
    BioCyci ARA:GQT-1115-MONOMER.
    ARA:GQT-1116-MONOMER.
    ARA:GQT-1117-MONOMER.
    ARA:GQT-1118-MONOMER.
    BRENDAi 2.1.2.1. 302.

    Gene expression databases

    ArrayExpressi Q8RXU4.
    Genevestigatori Q8RXU4.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR001597. ArAA_b-elim_lyase/Thr_aldolase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR023603. Threonine_aldolase.
    [Graphical view ]
    Pfami PF01212. Beta_elim_lyase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017617. Thr_aldolase. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    6. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    7. "Application of a high-throughput HPLC-MS/MS assay to Arabidopsis mutant screening; evidence that threonine aldolase plays a role in seed nutritional quality."
      Jander G., Norris S.R., Joshi V., Fraga M., Rugg A., Yu S., Li L., Last R.L.
      Plant J. 39:465-475(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF GLY-114.
    8. "Two Arabidopsis threonine aldolases are nonredundant and compete with threonine deaminase for a common substrate pool."
      Joshi V., Laubengayer K.M., Schauer N., Fernie A.R., Jander G.
      Plant Cell 18:3564-3575(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-114.

    Entry informationi

    Entry nameiTHA1_ARATH
    AccessioniPrimary (citable) accession number: Q8RXU4
    Secondary accession number(s): B9DG61
    , Q2V4P5, Q8LC88, Q9FRS2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3