Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative beta-glucosidase 5

Gene

BGLU5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein uncertaini

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431SubstrateBy similarity
Binding sitei140 – 1401SubstrateBy similarity
Binding sitei185 – 1851SubstrateBy similarity
Active sitei186 – 1861Proton donorBy similarity
Binding sitei328 – 3281SubstrateBy similarity
Active sitei395 – 3951NucleophileBy similarity
Binding sitei434 – 4341SubstrateBy similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT1G60260-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative beta-glucosidase 5 (EC:3.2.1.21)
Short name:
AtBGLU5
Gene namesi
Name:BGLU5
Ordered Locus Names:At1g60260
ORF Names:T13D8.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G60260.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 500480Putative beta-glucosidase 5PRO_0000389567Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi205 ↔ 212By similarity
Glycosylationi216 – 2161N-linked (GlcNAc...)Sequence Analysis
Glycosylationi361 – 3611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi424 – 4241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi495 – 4951N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ8RXN9.

Expressioni

Gene expression databases

GenevestigatoriQ8RXN9.

Structurei

3D structure databases

ProteinModelPortaliQ8RXN9.
SMRiQ8RXN9. Positions 25-477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni441 – 4422Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000088630.
InParanoidiQ8RXN9.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8RXN9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEQFFALFTI FLSFAFPGRC SDVFSRSDFP EGFLFGAGTS AYQWEGAAAE
60 70 80 90 100
DGRKPSVWDT LCYSRNIGNG DVTCDGYHKY KEDVKLMVDT NLDAFRFSIS
110 120 130 140 150
WSRLIPNGRG SVNQKGLQFY KNLISELITH GIEPHVTLYH YDHPQYLEDE
160 170 180 190 200
YGGWVNNMMI KDFTAYVDVC FREFGNYVKF WTTINEANVF TIGGYNDGDT
210 220 230 240 250
PPGRCSLPGK NCLLGNSSTE TYIVGHNLLL AHASASRLYK QKYKDKQGGS
260 270 280 290 300
IGFGLYLMGL TPSTSSKDDA IATQRAKDFY FGWFLGPLIF GDYPDTMKRT
310 320 330 340 350
IGSRLPVFSE EESEQVKGSS DFIGINHYFA ASVTNIKFKP SISGNPDFYS
360 370 380 390 400
DMGAYVTYLG NFSVIEYPVA PWTMEAVLEY IKQSYDNPPV YILENGTPMT
410 420 430 440 450
QHKDTHRVEY MNAYIGGVLK SIRNGSDTRG YFVWSFMDLF ELIGRYDYGY
460 470 480 490 500
GLYSVNFSDP HRKRSPRLSA HWYSDFLKGK TSFLDSKGIK ELQSNFSSSS
Length:500
Mass (Da):56,645
Last modified:February 7, 2011 - v2
Checksum:i2F6ECBC2A0607D5B
GO
Isoform 2 (identifier: Q8RXN9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     284-500: Missing.

Note: May be due to introns retention. No experimental confirmation available.

Show »
Length:283
Mass (Da):31,908
Checksum:i8EC851972BEF1D35
GO

Sequence cautioni

The sequence AAC24061.1 differs from that shown. Reason: Erroneous termination at position 328. Translated as Tyr.Curated
The sequence AAC24061.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AEE33671.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti184 – 1841I → V in AAL87256 (PubMed:14593172).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei284 – 500217Missing in isoform 2. 1 PublicationVSP_040524Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004473 Genomic DNA. Translation: AAC24061.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33671.1. Sequence problems.
AY080772 mRNA. Translation: AAL87256.1.
PIRiT02278.
RefSeqiNP_176232.2. NM_104716.2.
UniGeneiAt.36686.

Genome annotation databases

EnsemblPlantsiAT1G60260.1; AT1G60260.1; AT1G60260.
GeneIDi3767578.
KEGGiath:AT1G60260.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004473 Genomic DNA. Translation: AAC24061.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33671.1. Sequence problems.
AY080772 mRNA. Translation: AAL87256.1.
PIRiT02278.
RefSeqiNP_176232.2. NM_104716.2.
UniGeneiAt.36686.

3D structure databases

ProteinModelPortaliQ8RXN9.
SMRiQ8RXN9. Positions 25-477.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEiQ8RXN9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G60260.1; AT1G60260.1; AT1G60260.
GeneIDi3767578.
KEGGiath:AT1G60260.

Organism-specific databases

TAIRiAT1G60260.

Phylogenomic databases

HOGENOMiHOG000088630.
InParanoidiQ8RXN9.

Enzyme and pathway databases

BioCyciARA:AT1G60260-MONOMER.

Gene expression databases

GenevestigatoriQ8RXN9.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  4. Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGL05_ARATH
AccessioniPrimary (citable) accession number: Q8RXN9
Secondary accession number(s): F4IER4, O80749
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2009
Last sequence update: February 7, 2011
Last modified: January 6, 2015
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Could be the product of a pseudogene.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.