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Q8RXN9 (BGL05_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative beta-glucosidase 5

Short name=AtBGLU5
EC=3.2.1.21
Gene names
Name:BGLU5
Ordered Locus Names:At1g60260
ORF Names:T13D8.15
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceUncertain

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Caution

Could be the product of a pseudogene.

Sequence caution

The sequence AAC24061.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAC24061.1 differs from that shown. Reason: Erroneous termination at position 328. Translated as Tyr.

The sequence AEE33671.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8RXN9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8RXN9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     284-500: Missing.
Note: May be due to introns retention. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 500480Putative beta-glucosidase 5
PRO_0000389567

Regions

Region441 – 4422Substrate binding By similarity

Sites

Active site1861Proton donor By similarity
Active site3951Nucleophile By similarity
Binding site431Substrate By similarity
Binding site1401Substrate By similarity
Binding site1851Substrate By similarity
Binding site3281Substrate By similarity
Binding site4341Substrate By similarity

Amino acid modifications

Glycosylation2161N-linked (GlcNAc...) Potential
Glycosylation3611N-linked (GlcNAc...) Potential
Glycosylation4241N-linked (GlcNAc...) Potential
Glycosylation4561N-linked (GlcNAc...) Potential
Glycosylation4951N-linked (GlcNAc...) Potential
Disulfide bond205 ↔ 212 By similarity

Natural variations

Alternative sequence284 – 500217Missing in isoform 2.
VSP_040524

Experimental info

Sequence conflict1841I → V in AAL87256. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 8, 2011. Version 2.
Checksum: 2F6ECBC2A0607D5B

FASTA50056,645
        10         20         30         40         50         60 
MEQFFALFTI FLSFAFPGRC SDVFSRSDFP EGFLFGAGTS AYQWEGAAAE DGRKPSVWDT 

        70         80         90        100        110        120 
LCYSRNIGNG DVTCDGYHKY KEDVKLMVDT NLDAFRFSIS WSRLIPNGRG SVNQKGLQFY 

       130        140        150        160        170        180 
KNLISELITH GIEPHVTLYH YDHPQYLEDE YGGWVNNMMI KDFTAYVDVC FREFGNYVKF 

       190        200        210        220        230        240 
WTTINEANVF TIGGYNDGDT PPGRCSLPGK NCLLGNSSTE TYIVGHNLLL AHASASRLYK 

       250        260        270        280        290        300 
QKYKDKQGGS IGFGLYLMGL TPSTSSKDDA IATQRAKDFY FGWFLGPLIF GDYPDTMKRT 

       310        320        330        340        350        360 
IGSRLPVFSE EESEQVKGSS DFIGINHYFA ASVTNIKFKP SISGNPDFYS DMGAYVTYLG 

       370        380        390        400        410        420 
NFSVIEYPVA PWTMEAVLEY IKQSYDNPPV YILENGTPMT QHKDTHRVEY MNAYIGGVLK 

       430        440        450        460        470        480 
SIRNGSDTRG YFVWSFMDLF ELIGRYDYGY GLYSVNFSDP HRKRSPRLSA HWYSDFLKGK 

       490        500 
TSFLDSKGIK ELQSNFSSSS 

« Hide

Isoform 2 [UniParc].

Checksum: 8EC851972BEF1D35
Show »

FASTA28331,908

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[4]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1."
Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A.
Plant Mol. Biol. 55:343-367(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC004473 Genomic DNA. Translation: AAC24061.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33671.1. Sequence problems.
AY080772 mRNA. Translation: AAL87256.1.
PIRT02278.
RefSeqNP_176232.2. NM_104716.2.
UniGeneAt.36686.

3D structure databases

ProteinModelPortalQ8RXN9.
SMRQ8RXN9. Positions 21-482.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEQ8RXN9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G60260.1; AT1G60260.1; AT1G60260.
GeneID3767578.
KEGGath:AT1G60260.

Organism-specific databases

TAIRAT1G60260.

Phylogenomic databases

HOGENOMHOG000088630.

Enzyme and pathway databases

BioCycARA:AT1G60260-MONOMER.

Gene expression databases

GenevestigatorQ8RXN9.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGL05_ARATH
AccessionPrimary (citable) accession number: Q8RXN9
Secondary accession number(s): F4IER4, O80749
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: February 8, 2011
Last modified: June 11, 2014
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names