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Q8RXN9

- BGL05_ARATH

UniProt

Q8RXN9 - BGL05_ARATH

Protein

Putative beta-glucosidase 5

Gene

BGLU5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Protein uncertaini
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 2 (08 Feb 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei43 – 431SubstrateBy similarity
    Binding sitei140 – 1401SubstrateBy similarity
    Binding sitei185 – 1851SubstrateBy similarity
    Active sitei186 – 1861Proton donorBy similarity
    Binding sitei328 – 3281SubstrateBy similarity
    Active sitei395 – 3951NucleophileBy similarity
    Binding sitei434 – 4341SubstrateBy similarity

    GO - Molecular functioni

    1. beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT1G60260-MONOMER.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative beta-glucosidase 5 (EC:3.2.1.21)
    Short name:
    AtBGLU5
    Gene namesi
    Name:BGLU5
    Ordered Locus Names:At1g60260
    ORF Names:T13D8.15
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G60260.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 500480Putative beta-glucosidase 5PRO_0000389567Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi205 ↔ 212By similarity
    Glycosylationi216 – 2161N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi361 – 3611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi424 – 4241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi495 – 4951N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ8RXN9.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8RXN9.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8RXN9.
    SMRiQ8RXN9. Positions 21-482.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni441 – 4422Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000088630.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8RXN9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MEQFFALFTI FLSFAFPGRC SDVFSRSDFP EGFLFGAGTS AYQWEGAAAE    50
    DGRKPSVWDT LCYSRNIGNG DVTCDGYHKY KEDVKLMVDT NLDAFRFSIS 100
    WSRLIPNGRG SVNQKGLQFY KNLISELITH GIEPHVTLYH YDHPQYLEDE 150
    YGGWVNNMMI KDFTAYVDVC FREFGNYVKF WTTINEANVF TIGGYNDGDT 200
    PPGRCSLPGK NCLLGNSSTE TYIVGHNLLL AHASASRLYK QKYKDKQGGS 250
    IGFGLYLMGL TPSTSSKDDA IATQRAKDFY FGWFLGPLIF GDYPDTMKRT 300
    IGSRLPVFSE EESEQVKGSS DFIGINHYFA ASVTNIKFKP SISGNPDFYS 350
    DMGAYVTYLG NFSVIEYPVA PWTMEAVLEY IKQSYDNPPV YILENGTPMT 400
    QHKDTHRVEY MNAYIGGVLK SIRNGSDTRG YFVWSFMDLF ELIGRYDYGY 450
    GLYSVNFSDP HRKRSPRLSA HWYSDFLKGK TSFLDSKGIK ELQSNFSSSS 500
    Length:500
    Mass (Da):56,645
    Last modified:February 8, 2011 - v2
    Checksum:i2F6ECBC2A0607D5B
    GO
    Isoform 2 (identifier: Q8RXN9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         284-500: Missing.

    Note: May be due to introns retention. No experimental confirmation available.

    Show »
    Length:283
    Mass (Da):31,908
    Checksum:i8EC851972BEF1D35
    GO

    Sequence cautioni

    The sequence AAC24061.1 differs from that shown. Reason: Erroneous termination at position 328. Translated as Tyr.
    The sequence AAC24061.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence AEE33671.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti184 – 1841I → V in AAL87256. (PubMed:14593172)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei284 – 500217Missing in isoform 2. 1 PublicationVSP_040524Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004473 Genomic DNA. Translation: AAC24061.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE33671.1. Sequence problems.
    AY080772 mRNA. Translation: AAL87256.1.
    PIRiT02278.
    RefSeqiNP_176232.2. NM_104716.2.
    UniGeneiAt.36686.

    Genome annotation databases

    EnsemblPlantsiAT1G60260.1; AT1G60260.1; AT1G60260.
    GeneIDi3767578.
    KEGGiath:AT1G60260.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004473 Genomic DNA. Translation: AAC24061.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE33671.1 . Sequence problems.
    AY080772 mRNA. Translation: AAL87256.1 .
    PIRi T02278.
    RefSeqi NP_176232.2. NM_104716.2.
    UniGenei At.36686.

    3D structure databases

    ProteinModelPortali Q8RXN9.
    SMRi Q8RXN9. Positions 21-482.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PRIDEi Q8RXN9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G60260.1 ; AT1G60260.1 ; AT1G60260 .
    GeneIDi 3767578.
    KEGGi ath:AT1G60260.

    Organism-specific databases

    TAIRi AT1G60260.

    Phylogenomic databases

    HOGENOMi HOG000088630.

    Enzyme and pathway databases

    BioCyci ARA:AT1G60260-MONOMER.

    Gene expression databases

    Genevestigatori Q8RXN9.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    4. Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiBGL05_ARATH
    AccessioniPrimary (citable) accession number: Q8RXN9
    Secondary accession number(s): F4IER4, O80749
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: February 8, 2011
    Last modified: October 1, 2014
    This is version 62 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3