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Protein

Putative beta-glucosidase 5

Gene

BGLU5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Protein uncertaini

Functioni

Caution

Could be the product of a pseudogene.Curated

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43SubstrateBy similarity1
Binding sitei140SubstrateBy similarity1
Binding sitei185SubstrateBy similarity1
Active sitei186Proton donorBy similarity1
Binding sitei328SubstrateBy similarity1
Active sitei395NucleophileBy similarity1
Binding sitei434SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT1G60260-MONOMER
ReactomeiR-ATH-189085 Digestion of dietary carbohydrate

Protein family/group databases

CAZyiGH1 Glycoside Hydrolase Family 1

Names & Taxonomyi

Protein namesi
Recommended name:
Putative beta-glucosidase 5 (EC:3.2.1.21)
Short name:
AtBGLU5
Gene namesi
Name:BGLU5
Ordered Locus Names:At1g60260
ORF Names:T13D8.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G60260

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000038956721 – 500Putative beta-glucosidase 5Add BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi205 ↔ 212By similarity
Glycosylationi216N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi361N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi424N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi456N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi495N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8RXN9
PRIDEiQ8RXN9

Expressioni

Gene expression databases

ExpressionAtlasiQ8RXN9 differential
GenevisibleiQ8RXN9 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G60260.1

Structurei

3D structure databases

ProteinModelPortaliQ8RXN9
SMRiQ8RXN9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni441 – 442Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0626 Eukaryota
COG2723 LUCA
HOGENOMiHOG000088630
InParanoidiQ8RXN9
KOiK01188
OrthoDBiEOG0936071L

Family and domain databases

InterProiView protein in InterPro
IPR001360 Glyco_hydro_1
IPR033132 Glyco_hydro_1_N_CS
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR10353 PTHR10353, 1 hit
PfamiView protein in Pfam
PF00232 Glyco_hydro_1, 1 hit
PRINTSiPR00131 GLHYDRLASE1
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00653 GLYCOSYL_HYDROL_F1_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8RXN9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEQFFALFTI FLSFAFPGRC SDVFSRSDFP EGFLFGAGTS AYQWEGAAAE
60 70 80 90 100
DGRKPSVWDT LCYSRNIGNG DVTCDGYHKY KEDVKLMVDT NLDAFRFSIS
110 120 130 140 150
WSRLIPNGRG SVNQKGLQFY KNLISELITH GIEPHVTLYH YDHPQYLEDE
160 170 180 190 200
YGGWVNNMMI KDFTAYVDVC FREFGNYVKF WTTINEANVF TIGGYNDGDT
210 220 230 240 250
PPGRCSLPGK NCLLGNSSTE TYIVGHNLLL AHASASRLYK QKYKDKQGGS
260 270 280 290 300
IGFGLYLMGL TPSTSSKDDA IATQRAKDFY FGWFLGPLIF GDYPDTMKRT
310 320 330 340 350
IGSRLPVFSE EESEQVKGSS DFIGINHYFA ASVTNIKFKP SISGNPDFYS
360 370 380 390 400
DMGAYVTYLG NFSVIEYPVA PWTMEAVLEY IKQSYDNPPV YILENGTPMT
410 420 430 440 450
QHKDTHRVEY MNAYIGGVLK SIRNGSDTRG YFVWSFMDLF ELIGRYDYGY
460 470 480 490 500
GLYSVNFSDP HRKRSPRLSA HWYSDFLKGK TSFLDSKGIK ELQSNFSSSS
Length:500
Mass (Da):56,645
Last modified:February 8, 2011 - v2
Checksum:i2F6ECBC2A0607D5B
GO
Isoform 2 (identifier: Q8RXN9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     284-500: Missing.

Note: May be due to introns retention. No experimental confirmation available.
Show »
Length:283
Mass (Da):31,908
Checksum:i8EC851972BEF1D35
GO

Sequence cautioni

The sequence AAC24061 differs from that shown. Reason: Erroneous termination at position 328. Translated as Tyr.Curated
The sequence AAC24061 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184I → V in AAL87256 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040524284 – 500Missing in isoform 2. 1 PublicationAdd BLAST217

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004473 Genomic DNA Translation: AAC24061.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE33671.2
AY080772 mRNA Translation: AAL87256.1
PIRiT02278
RefSeqiNP_001319275.1, NM_001333890.1
UniGeneiAt.36686

Genome annotation databases

GeneIDi3767578
KEGGiath:AT1G60260

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiBGL05_ARATH
AccessioniPrimary (citable) accession number: Q8RXN9
Secondary accession number(s): F4IER4, O80749
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: February 8, 2011
Last modified: May 23, 2018
This is version 83 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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