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Q8RXN3 (PPT1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate/phosphate translocator 1, chloroplastic
Short name=AtPPT1
Alternative name(s):
Protein CAB UNDEREXPRESSED 1
Gene names
Name:PPT1
Synonyms:CUE1
Ordered Locus Names:At5g33320
ORF Names:F19N2.40
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length408 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphoenolpyruvate/phosphate translocator that transports phosphoenolpyruvate (PEP), 2-phosphoglycerate, 3-phosphoglycerate and dihydroxyacetone phosphate. Imports PEP to the chloroplast stroma as one substrate of the shikimate pathway, from which aromatic amino acids and a variety of secondary products derive. Required for correct leaf mesophyll cell development and expression of chlorophyll a/b binding protein 3 (CAB3). Ref.6 Ref.7 Ref.8 Ref.9

Subcellular location

Plastid › chloroplast membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in root columella, lateral root cap and root vasculature tissue. In leaves, highly expressed in xylem parenchyma cells. In flowers, expressed in sepals, petals, filaments of the stamens, anthers and stigma. Ref.8

Disruption phenotype

Reticulate leaf phenotype. Reduced number of leaf palisade mesophyll cells and increased bundle sheath cells. Small chloroplasts in mesophyll cells. Reduced photosynthetic electron transport rate. Under-expression of CAB3 protein. Reduced levels of aromatic amino acids and phenolic compounds derived from shikimate pathway. Ref.7 Ref.8

Sequence similarities

Belongs to the TPT transporter family. PPT (TC 2.A.7.9) subfamily. [View classification]

Contains 1 EamA domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3030Chloroplast Potential
Chain31 – 408378Phosphoenolpyruvate/phosphate translocator 1, chloroplastic
PRO_0000406099

Regions

Transmembrane105 – 12521Helical; Potential
Transmembrane137 – 15721Helical; Potential
Transmembrane165 – 18521Helical; Potential
Transmembrane198 – 21821Helical; Potential
Transmembrane222 – 24221Helical; Potential
Transmembrane283 – 30321Helical; Potential
Transmembrane324 – 34623Helical; Potential
Transmembrane377 – 39620Helical; Potential
Domain124 – 241118EamA

Experimental info

Mutagenesis2511G → E in cue1-7; strong reticulate leaf phenotype. Ref.6
Mutagenesis2651R → C in cue1-5; weak reticulate leaf phenotype. Ref.6
Mutagenesis3881A → V in cue1-3; weak reticulate leaf phenotype. Ref.6
Sequence conflict1531I → T in AAF63704. Ref.1
Sequence conflict1911L → I in AAB40646. Ref.1
Sequence conflict3221K → Q in AAM63308. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q8RXN3 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 4C912B12D77CE5F6

FASTA40844,224
        10         20         30         40         50         60 
MQSSAVFSLS PSLPLLKPRR LSLRHHPITT AASSSDLNVS PNVVSIPSLS RRSWRLASSD 

        70         80         90        100        110        120 
SPLRAWSGVP SPISHSLDTN RFRTAATAVP ESAEEGDNSG KLTKVLELGL LFAMWYLFNI 

       130        140        150        160        170        180 
YFNIYNKQVL KALHAPMTVT LVQFAVGSVL ITIMWVLNLY KRPKISGAQL AAILPLAVVH 

       190        200        210        220        230        240 
TLGNLFTNMS LGKVSVSFTH TIKAMEPFFS VLLSAMFLGE KPTPWVLGAI VPIVGGVALA 

       250        260        270        280        290        300 
SISEVSFNWA GFSSAMASNL TNQSRNVLSK KVMVKKDDSL DNITLFSIIT LMSLVLMAPV 

       310        320        330        340        350        360 
TFFTEGIKFT PSYIQSAGVN VKQIYTKSLI AALCFHAYQQ VSYMILARVS PVTHSVGNCV 

       370        380        390        400 
KRVVVIVSSV IFFKTPVSPV NAFGTGIALA GVFLYSRVKG IKPKPKTA

« Hide

References

« Hide 'large scale' references
[1]"The phosphate/phosphoenolpyruvate translocator gene from Arabidopsis."
Deterding S., Fluegge U.-I., Fischer K.
Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"The phosphoenolpyruvate/phosphate translocator is required for phenolic metabolism, palisade cell development, and plastid-dependent nuclear gene expression."
Streatfield S.J., Weber A., Kinsman E.A., Haeusler R.E., Li J., Post-Beittenmiller D., Kaiser W.M., Pyke K.A., Fluegge U.I., Chory J.
Plant Cell 11:1609-1622(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF GLY-251; ARG-265 AND ALA-388.
[7]"The phenotype of the Arabidopsis cue1 mutant is not simply caused by a general restriction of the shikimate pathway."
Voll L., Haeusler R.E., Hecker R., Weber A., Weissenboeck G., Fiene G., Waffenschmidt S., Fluegge U.I.
Plant J. 36:301-317(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[8]"Characterization of two functional phosphoenolpyruvate/phosphate translocator (PPT) genes in Arabidopsis--AtPPT1 may be involved in the provision of signals for correct mesophyll development."
Knappe S., Loettgert T., Schneider A., Voll L., Fluegge U.I., Fischer K.
Plant J. 36:411-420(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[9]"A cell-free translation and proteoliposome reconstitution system for functional analysis of plant solute transporters."
Nozawa A., Nanamiya H., Miyata T., Linka N., Endo Y., Weber A.P., Tozawa Y.
Plant Cell Physiol. 48:1815-1820(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U66321 mRNA. Translation: AAB40646.1.
AF209210 Genomic DNA. Translation: AAF63704.1.
AC051625 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93894.1.
AY080788 mRNA. Translation: AAL87271.1.
AY133809 mRNA. Translation: AAM91743.1.
AY086100 mRNA. Translation: AAM63308.1.
IPIIPI00534885.
RefSeqNP_198317.1. NM_122856.2.
UniGeneAt.14730.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING3702.AT5G33320.1-P.

Proteomic databases

PaxDbQ8RXN3.
PRIDEQ8RXN3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G33320.1; AT5G33320.1; AT5G33320.
GeneID833308.
KEGGath:AT5G33320.

Organism-specific databases

TAIRAt5g33320.

Phylogenomic databases

eggNOGNOG312636.
HOGENOMHOG000241297.
InParanoidQ8RXN3.
KOK15283.
OMAFNAVIFM.
PhylomeDBQ8RXN3.
ProtClustDBCLSN2720506.

Gene expression databases

GenevestigatorQ8RXN3.

Family and domain databases

InterProIPR000620. DMT.
IPR004853. DUF250.
IPR004696. Tpt_PEP_transl.
[Graphical view]
PfamPF00892. EamA. 1 hit.
PF03151. TPT. 1 hit.
[Graphical view]
TIGRFAMsTIGR00817. tpt. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPT1_ARATH
AccessionPrimary (citable) accession number: Q8RXN3
Secondary accession number(s): P92991, Q8LDB3, Q9M669
Entry history
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: June 1, 2002
Last modified: May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents